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1.
Anal Bioanal Chem ; 415(23): 5735-5743, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37453938

RESUMO

Wavelength interrogation surface plasmon resonance imaging (WSPRi) sensing has unique advantages in high-throughput imaging detection. The refractive index resolution (RIR) of WSPRi is limited to the order of 10-6 RIU. This paper demonstrates a novel WSPRi sensing system with a wavelength scanning device of an acousto-optic tunable filter (AOTF) and a low-cost speckle-free SPR excitation source of a halogen lamp. We developed a sensitive quasi-phase extraction method for data processing. The new technique achieved an RIR of 8.84×10-7 RIU, which is the first WSPRi system that has an RIR in the order of 10-7 RIU. Moreover, we performed a real-time recording of the formation of the coffee ring effect during brine evaporation and enhanced the biosensor performance of SPR for the first time. We believe the higher RIR and accuracy of the system will benefit more potential applications toward exploring the biomolecules' behaviors in biological and biochemistry studies.


Assuntos
Técnicas Biossensoriais , Ressonância de Plasmônio de Superfície , Ressonância de Plasmônio de Superfície/métodos , Técnicas Biossensoriais/métodos , Óptica e Fotônica , Refratometria , Diagnóstico por Imagem
2.
Biosensors (Basel) ; 14(4)2024 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-38667188

RESUMO

SPR biosensors have been extensively used for investigating protein-protein interactions. However, in conventional surface plasmon resonance (SPR) biosensors, detection is limited by the Brownian-motion-governed diffusion process of sample molecules in the sensor chip, which makes it challenging to detect biomolecule interactions at ultra-low concentrations. Here, we propose a highly sensitive SPR imaging biosensor which exploits the coffee ring effect (CRE) for in situ enrichment of molecules on the sensing surface. In addition, we designed a wavelength modulation system utilizing two LEDs to reduce the system cost and enhance the detection speed. Furthermore, a detection limit of 213 fM is achieved, which amounts to an approximately 365 times improvement compared to traditional SPR biosensors. With further development, we believe that this SPR imaging system with high sensitivity, less sample consumption, and faster detection speed can be readily applied to ultra-low-concentration molecular detection and interaction analysis.


Assuntos
Técnicas Biossensoriais , Ressonância de Plasmônio de Superfície , Limite de Detecção
3.
Biosensors (Basel) ; 13(10)2023 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-37887139

RESUMO

Intensity interrogation-based surface plasmon resonance imaging (ISPRi) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. In this study, we report the successful development of a novel high-sensitivity ISPRi biosensor and its application for apoptosis detection in cancer cells. By optimizing the excitation wavelength and excitation angle, we achieved a refractive index resolution (RIR) of 5.20 × 10-6 RIU. Importantly, the biosensor has been tested and validated for high-throughput and label-free detection of activated caspase-3 with its specific inhibitor Z-DEVD-FMK in apoptotic cells. Therefore, this study describes a novel molecular imaging system to monitor apoptosis in cancers for disease diagnosis and/or evaluation of therapeutic efficacy of anti-cancer drugs.


Assuntos
Técnicas Biossensoriais , Neoplasias , Humanos , Ressonância de Plasmônio de Superfície/métodos , Técnicas Biossensoriais/métodos , Neoplasias/diagnóstico , Refratometria , Apoptose
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