Analysis of Land Cover Changes in Afromontane Vegetation of Image Forest Reserve, Southern Highlands of Tanzania.

An analysis of land cover changes (LCCs) was done in Image Forest Reserve (IFR) from August to October 2019. Free satellite images for 1990, 2004, and 2018 were downloaded from Landsat 5 (TM) and Landsat 8 (OLI) available through the USGS portal. Ground surveys were conducted using systematically set plots of 20 m × 40 m to identify the existing land cover types and human illegal activities. Geographical coordinates for each of these plots were recorded using handheld GPS. We witnessed the changes of land cover types in the three decades. Forest had contracted, while shrubland and grassland and woodland had expanded within IFR. Between 1990 and 2004, woodland, bareland and rocky outcrops, shrubland, and grassland had consistently decreased though at a different rate of change, while forest has increased between the same assessment periods. The period of 2004-2018 has shown a consistent increase at different rates in woodland, bareland and rocky outcrops, shrubland, and grassland at a different rate of change, while forest has decreased between the same assessment periods. Further study is needed, bylaws and laws should be implemented, participatory forest management should be encouraged, beekeeping and ecotourism should be introduced, provision of regular education to the community by the Tanzania Forest Service (TFS) should be encouraged, and awareness creation should be made.

Key changes in gene expression identified for different stages of C4 evolution in Alloteropsis semialata.

C4 photosynthesis is a complex trait that boosts productivity in tropical conditions. Compared with C3 species, the C4 state seems to require numerous novelties, but species comparisons can be confounded by long divergence times. Here, we exploit the photosynthetic diversity that exists within a single species, the grass Alloteropsis semialata, to detect changes in gene expression associated with different photosynthetic phenotypes. Phylogenetically informed comparative transcriptomics show that intermediates with a weak C4 cycle are separated from the C3 phenotype by increases in the expression of 58 genes (0.22% of genes expressed in the leaves), including those encoding just three core C4 enzymes: aspartate aminotransferase, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate carboxylase. The subsequent transition to full C4 physiology was accompanied by increases in another 15 genes (0.06%), including only the core C4 enzyme pyruvate orthophosphate dikinase. These changes probably created a rudimentary C4 physiology, and isolated populations subsequently improved this emerging C4 physiology, resulting in a patchwork of expression for some C4 accessory genes. Our work shows how C4 assembly in A. semialata happened in incremental steps, each requiring few alterations over the previous step. These create short bridges across adaptive landscapes that probably facilitated the recurrent origins of C4 photosynthesis through a gradual process of evolution.

Madagascar's grasses and grasslands: anthropogenic or natural?

Grasses, by their high productivity even under very low pCO2, their ability to survive repeated burning and to tolerate long dry seasons, have transformed the terrestrial biomes in the Neogene and Quaternary. The expansion of grasslands at the cost of biodiverse forest biomes in Madagascar is often postulated as a consequence of the Holocene settlement of the island by humans. However, we show that the Malagasy grass flora has many indications of being ancient with a long local evolutionary history, much predating the Holocene arrival of humans. First, the level of endemism in the Madagascar grass flora is well above the global average for large islands. Second, a survey of many of the more diverse areas indicates that there is a very high spatial and ecological turnover in the grass flora, indicating a high degree of niche specialization. We also find some evidence that there are both recently disturbed and natural stable grasslands: phylogenetic community assembly indicates that recently severely disturbed grasslands are phylogenetically clustered, whereas more undisturbed grasslands tend to be phylogenetically more evenly distributed. From this evidence, it is likely that grass communities existed in Madagascar long before human arrival and so were determined by climate, natural grazing and other natural factors. Humans introduced zebu cattle farming and increased fire frequency, and may have triggered an expansion of the grasslands. Grasses probably played the same role in the modification of the Malagasy environments as elsewhere in the tropics.