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We propose a frequency-modulated continuous wave (FMCW) radar estimation algorithm with high resolution and low complexity. The fast Fourier transform (FFT)-based algorithms and multiple signal classification (MUSIC) algorithms are used as algorithms for estimating target parameters in the FMCW radar systems. FFT-based and MUSIC algorithms have tradeoff characteristics between resolution performance and complexity. While FFT-based algorithms have the advantage of very low complexity, they have the disadvantage of a low-resolution performance; that is, estimating multiple targets with similar parameters as a single target. On the other hand, subspace-based algorithms have the advantage of a high-resolution performance, but have a problem of very high complexity. In this paper, we propose an algorithm with reduced complexity, while achieving the high-resolution performance of the subspace-based algorithm by utilizing the advantages of the two algorithms; namely, the low-complexity advantage of FFT-based algorithms and the high-resolution performance of the MUSIC algorithms. The proposed algorithm first reduces the amount of data used as input to the subspace-based algorithm by using the estimation results obtained by FFT. Secondly, it significantly reduces the range of search regions considered for pseudo-spectrum calculations in the subspace-based algorithm. The simulation and experiment results show that the proposed algorithm achieves a similar performance compared with the conventional and low complexity MUSIC algorithms, despite its considerably lower complexity.
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This paper proposes a high-efficiency super-resolution frequency-modulated continuous-wave (FMCW) radar algorithm based on estimation by fast Fourier transform (FFT). In FMCW radar systems, the maximum number of samples is generally determined by the maximum detectable distance. However, targets are often closer than the maximum detectable distance. In this case, even if the number of samples is reduced, the ranges of targets can be estimated without degrading the performance. Based on this property, the proposed algorithm adaptively selects the number of samples used as input to the super-resolution algorithm depends on the coarsely estimated ranges of targets using the FFT. The proposed algorithm employs the reduced samples by the estimated distance by FFT as input to the super resolution algorithm instead of the maximum number of samples set by the maximum detectable distance. By doing so, the proposed algorithm achieves the similar performance of the conventional multiple signal classification algorithm (MUSIC), which is a representative of the super resolution algorithms while the performance does not degrade. Simulation results demonstrate the feasibility and performance improvement provided by the proposed algorithm; that is, the proposed algorithm achieves average complexity reduction of 88% compared to the conventional MUSIC algorithm while achieving its similar performance. Moreover, the improvement provided by the proposed algorithm was verified in practical conditions, as evidenced by our experimental results.
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MAF1 is a global suppressor of RNA polymerase III-dependent transcription, and is conserved from yeast to human. Growing evidence supports the involvement of MAF1 in the immune response of mammals, but its biological functions in fish are unknown. We isolated and characterized Maf1 from the olive flounder Paralichthys olivaceus (PoMaf1). The coding region of PoMaf1 comprised 738 bp encoding a 245-amino-acid protein. The deduced PoMAF1 amino acid sequence shared features with those of MAF1 orthologues from vertebrates. PoMaf1 mRNA was detected in all tissues examined, and the levels were highest in eye and muscle tissue. The PoMaf1 mRNA level increased during early development. In addition, the PoMaf1 transcript level decreased during viral hemorrhagic septicemia virus (VHSV) infection of flounder hirame natural embryo (HINAE) cells. To investigate the role of PoMaf1 in VHSV infection, single-cell-derived PoMaf1 knockout HINAE cells were generated using the clustered regularly interspaced short palindromic repeats/CRISPR-associated-9 (CRISPR/Cas9) system, and cell clones with complete disruption of PoMaf1 were selected. PoMaf1 disruption increased the VHSV glycoprotein (G) mRNA levels during VHSV infection of HINAE cells, implicating PoMAF1 in the immune response to VSHV infection. To our knowledge, this is the first study to characterize fish Maf1, which may play a role in the response to viral infection.
Assuntos
Doenças dos Peixes/genética , Proteínas de Peixes/genética , Linguado/genética , Septicemia Hemorrágica/veterinária , Novirhabdovirus/fisiologia , Proteínas Repressoras/genética , Animais , Sistemas CRISPR-Cas , Linhagem Celular , Doenças dos Peixes/imunologia , Proteínas de Peixes/imunologia , Linguado/imunologia , Linguado/fisiologia , Septicemia Hemorrágica/genética , Septicemia Hemorrágica/imunologia , Interações Hospedeiro-Patógeno , Novirhabdovirus/imunologia , Filogenia , Proteínas Repressoras/imunologia , Transcrição GênicaRESUMO
This paper proposes a low complexity multiple-signal-classifier (MUSIC)-based direction-of-arrival (DOA) detection algorithm for frequency-modulated continuous-wave (FMCW) vital radars. In order to reduce redundant complexity, the proposed algorithm employs characteristics of distance between adjacent arrays having trade-offs between field of view (FOV) and resolution performance. First, the proposed algorithm performs coarse DOA estimation using fast Fourier transform. On the basis of the coarse DOA estimation, the number of channels as input of the MUSIC algorithm are selected. If the estimated DOA is smaller than 30°, it implies that there is an FOV margin. Therefore, the proposed algorithm employs only half of the channels, that is, it is the same as doubling the spacing between arrays. By doing so, the proposed algorithm achieves more than 40% complexity reduction compared to the conventional MUSIC algorithm while achieving similar performance. By experiments, it is shown that the proposed algorithm despite the low complexity is enable to distinguish the adjacent DOA in a practical environment.
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Torreya nucifera is an evergreen tree in the family Taxaceae, the seeds, leaves, and stems of which have long been used as edible products and herbal medicines in Korea. Previous studies of biological activity have shown that T. nucifera has antioxidant and anti-inflammatory effects. However, the effect of T. nucifera leaves on melanogenesis are yet to be studied. In this investigation, we used B16F10 melanoma cells to test the efficacy of T. nucifera leaf hot water extract (TLWE). α-melanocyte stimulating hormone (α-MSH) stimulated B16F10 melanoma cells were treated with various concentrations of TLWE (50, 100, and 200 µg/mL). The results showed that TLWE reduced the melanin content and cellular tyrosinase activity in a concentration-dependent manner. It also inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38) and c-Jun N-terminal kinase (JNK) in the mitogen-activated protein kinase (MAPK) signaling pathway. The compounds catechin and ρ-coumaric acid, which are known to have a whitening effect on skin, were detected by HPLC analysis. These results suggest that TLWE has an anti-melanogenic effect. In addition, the safety of TLWE was tested. The results of the skin irritation test showed that TLWE is harmless to the human skin, even at higher concentrations than those used in the experiment. Therefore, we suggest that the water extract of T. nucifera leaves has potential for use as a skin-whitening agent.
Assuntos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Melaninas/antagonistas & inibidores , Extratos Vegetais/farmacologia , Taxaceae/química , Adulto , Animais , AMP Cíclico/metabolismo , Relação Dose-Resposta a Droga , Feminino , Temperatura Alta , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Masculino , Melaninas/metabolismo , Melanoma Experimental/metabolismo , Camundongos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/toxicidade , Folhas de Planta , Transdução de Sinais/efeitos dos fármacos , Testes de Irritação da Pele , alfa-MSH , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismoRESUMO
Background: Melanin in the skin is the defense against the harmful UV radiation, which is considered as one of the major risk factors for skin cancer. The compound 7,8-dimethoxycoumarin (DMC, C11H10O4), a natural coumarin molecule present in several medicinal plants, possesses antioxidant and anti-inflammatory activities. However, the mechanism underlying its effects on melanogenesis in melanocytes is unclear. Therefore, we investigated the effect of DMC on melanogenesis activation in B16F10 melanoma cells. Methods: We examined the cytotoxic range of DMC on B16F10 melanoma cells and increased effects of melanogenesis, and intracellular tyrosinase activity. In addition, regulation mechanisms were assessed by Western blot analysis. Results: The results showed that DMC significantly increased melanin content and tyrosinase activity in the cells without being cytotoxic. Furthermore, DMC stimulated the expression of tyrosinase, TRP-1, TRP-2, and MITF thereby activating melanin production and Akt phosphorylation was increased in the Akt signaling pathway. on the contrary, interfering with the phosphorylation of ERK in the MAPKs pathway. Conclusions: These results suggest that DMC may serve as a candidate for potential melanin-producing activator and anti-gray hair applications.
Assuntos
Cumarínicos/farmacologia , Melaninas/biossíntese , Fator de Transcrição Associado à Microftalmia/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Animais , Linhagem Celular Tumoral , Sobrevivência Celular , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Oxirredutases Intramoleculares/metabolismo , Melanócitos/efeitos dos fármacos , Melanócitos/metabolismo , Melanoma Experimental , Glicoproteínas de Membrana/metabolismo , Camundongos , Monofenol Mono-Oxigenase/metabolismo , Oxirredutases/metabolismoRESUMO
This paper proposes a low-complexity frequency-modulated continuous wave (FMCW) surveillance radar algorithm using random dual chirps in order to overcome the blind-speed problem and reduce the computational complexity. In surveillance radar algorithm, the most widely used moving target indicator (MTI) algorithm is proposed to effectively remove clutter. However, the MTI algorithm has a so-called 'blind-speed problem' that cannot detect a target of a specific velocity. In this paper, we try to solve the blind-speed problem of MTI algorithm by randomly selecting two beat signals selected for MTI for each frame. To further reduce the redundant complexity, the proposed algorithm first performs one-dimensional fast Fourier transform (FFT) for range detection and performs multidimensional FFT only when it is determined that a target exists at each frame. The simulation results show that despite low complexity, the proposed algorithm detects moving targets well by avoiding the problem of blind speed. Furthermore, the effectiveness of the proposed algorithm was verified by performing an experiment using the FMCW radar system in a real environment.
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A low-complexity joint range and Doppler frequency-modulated continuous wave (FMCW) radar algorithm based on the number of targets is proposed in this paper. This paper introduces two low-complexity FMCW radar algorithms, that is, region of interest (ROI)-based and partial discrete Fourier transform (DFT)-based algorithms. We find the low-complexity condition of each algorithm by analyzing the complexity of these algorithms. From this analysis, it is found that the number of targets is an important factor in determining complexity. Based on this result, the proposed algorithm selects a low-complexity algorithm between two algorithms depending the estimated number of targets and thus achieves lower complexity compared two low-complexity algorithms introduced. The experimental results using real FMCW radar systems show that the proposed algorithm works well in a real environment. Moreover, central process unit time and count of float pointing are shown as a measure of complexity.
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Horse oil products have been used in skin care for a long time in traditional medicine, but the biological effects of horse oil on the skin remain unclear. This study was conducted to evaluate the protective effect of horse oil on ultraviolet B (UVB)-induced oxidative stress in human HaCaT keratinocytes. Horse oil significantly reduced UVB-induced intracellular reactive oxygen species and intracellular oxidative damage to lipids, proteins, and DNA. Horse oil absorbed light in the UVB range of the electromagnetic spectrum and suppressed the generation of cyclobutane pyrimidine dimers, a photoproduct of UVB irradiation. Western blotting showed that horse oil increased the UVB-induced Bcl-2/Bax ratio, inhibited mitochondria-mediated apoptosis and matrix metalloproteinase expression, and altered mitogen-activated protein kinase signaling-related proteins. These effects were conferred by increased phosphorylation of extracellular signal-regulated kinase 1/2 and decreased phosphorylation of p38 and c-Jun N-terminal kinase 1/2. Additionally, horse oil reduced UVB-induced binding of activator protein 1 to the matrix metalloproteinase-1 promoter site. These results indicate that horse oil protects human HaCaT keratinocytes from UVB-induced oxidative stress by absorbing UVB radiation and removing reactive oxygen species, thereby protecting cells from structural damage and preventing cell death and aging. In conclusion, horse oil is a potential skin protectant against skin damage involving oxidative stress.
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Queratinócitos/patologia , Queratinócitos/efeitos da radiação , Óleos/farmacologia , Estresse Oxidativo/efeitos da radiação , Raios Ultravioleta , Absorção de Radiação , Animais , Apoptose/efeitos da radiação , Linhagem Celular , Ativação Enzimática/efeitos da radiação , Cavalos , Humanos , Queratinócitos/enzimologia , Sistema de Sinalização das MAP Quinases/efeitos da radiação , Metaloproteinases da Matriz/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
We determined the molecular mechanism underlying the environmental (photoperiodic) regulation of sexual maturation in fish, we examined the expression of sexual maturation-related hormones and vertebrate ancient long opsin (VAL-opsin) in goldfish (Carassius auratus) exposed to different light spectra (red and green light-emitting diodes). We further evaluated the effect of exogenous gonadotropin hormone (GTH) on the expression of VAL-opsin under different light conditions. Our results demonstrated that the expression of GTHs was higher in the fish exposed to green light, and VAL-opsin levels were increased in the fish receiving GTH injection. Therefore, we have uncovered a molecular mechanism underlying the environmental (light)-induced trigger for sexual maturation: VAL-opsin is activated by green light and GTH, which promotes the expression of sexual maturation genes.
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Proteínas de Peixes/metabolismo , Carpa Dourada/crescimento & desenvolvimento , Carpa Dourada/metabolismo , Gonadotropinas Hipofisárias/metabolismo , Opsinas/metabolismo , Maturidade Sexual/efeitos da radiação , Animais , Proteínas de Peixes/genética , Expressão Gênica/efeitos da radiação , Carpa Dourada/genética , Gonadotropinas Hipofisárias/genética , Luz , Opsinas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Maturidade Sexual/genética , Maturidade Sexual/fisiologiaRESUMO
We tested the mitigating effects of melatonin injections or irradiation from green-wavelength light-emitting diodes (LEDs) on goldfish (Carassius auratus) exposed to thermal stress (high water temperature, 30 °C). The effects of the two treatments were assessed by measuring the expression and activity levels of the antioxidant enzymes, superoxide dismutase and catalase, plasma hydrogen peroxide, lipid hydroperoxide, and lysozyme. In addition, a comet assay was conducted to confirm that high water temperature damaged nuclear DNA. The expression and activity of the antioxidant enzymes, plasma hydrogen peroxide, and lipid hydroperoxide were significantly higher after exposure to high temperature and were significantly lower in fish that received melatonin or LED light than in those that received no mitigating treatment. Plasma lysozyme was significantly lower after exposure to high temperature and was significantly higher after exposure to melatonin or LED light. The comet assay revealed that thermal stress caused a great deal of damage to nuclear DNA; however, treatment with melatonin or green-wavelength LED light prevented a significant portion of this damage from occurring. These results indicate that, although high temperatures induce oxidative stress and reduce immune system strength in goldfish, both melatonin and green-wavelength LED light inhibit oxidative stress and boost the immune system. LED treatment increased the antioxidant and immune system activity more significantly than did melatonin treatment.
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Carpa Dourada/imunologia , Temperatura Alta , Luz , Melatonina/farmacologia , Animais , Catalase/genética , Catalase/metabolismo , Ativação Enzimática/efeitos dos fármacos , Ativação Enzimática/efeitos da radiação , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Regulação Enzimológica da Expressão Gênica/efeitos da radiação , Carpa Dourada/genética , Peróxido de Hidrogênio/sangue , Peróxidos Lipídicos/sangue , Estresse Oxidativo , Oxirredutases/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Distribuição Aleatória , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/efeitos da radiação , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismoRESUMO
We investigated how different light spectra affect thermal stress in olive flounder (Paralichthys olivaceus), using light emitting diodes (LEDs; blue, 450 nm; green, 530 nm; red, 630 nm) at two intensities (0.3 and 0.5 W/m(2)) at relatively high water temperatures (25 and 30 °C, compared to a control condition of 20 °C). We measured the expression and activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), and the levels of plasma hydrogen peroxide (H2O2) and lipid peroxidation (LPO). Furthermore, the levels and mRNA expression of caspase-3 were measured, and terminal transferase dUTP nick end labeling (TUNEL) assays of liver and comet assays were performed. The expression and activity of antioxidant enzymes, as well as plasma H2O2 and LPO levels were significantly higher after exposure to high temperatures, and significantly lower after exposure to green and blue light. Caspase-3 levels and mRNA expression showed a similar pattern. The TUNEL assay showed that apoptosis markedly increased at higher water temperatures, compared with the 20 °C control. In contrast, green light irradiation decreased apoptosis rate. Furthermore, the comet assays showed that nuclear DNA damage was caused by thermal stress, and that green light irradiation played a role in partially preventing this damage. Overall, these results suggest that light with green and blue wavelengths can reduce both high temperature-induced oxidative stress and apoptosis, and that particularly green light is efficient for this. Therefore, green light can play a role in protecting in olive flounder from thermal stress damage.
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Apoptose/efeitos da radiação , Linguados/fisiologia , Temperatura Alta , Imunidade Inata , Luz , Estresse Oxidativo/efeitos da radiação , Animais , Água/análiseRESUMO
Photoperiod is considered the most important factor that entrains animal rhythms, including the reproductive cycle. The present study tested differences in sex maturation and sex steroid hormones of yellowtail damselfish (Chrysiptera parasema) exposed to a white fluorescent bulb (12L:12D and 14L:10D) or long-afterglow phosphorescent pigment (LumiNova sheet) for 4 months. At the end of the experiment, in the phosphorescent group, mRNA expressions of gonadotropin hormones [(GTHs, including gonadotropin (GTH) α and luteinizing hormone (LH) ß)], estrogen receptor (ER), and vitellogenin were significantly higher than in the photoperiod groups (12L:12D and 14L:10D), and these results are consistent with those of Western blotting for protein expression. Furthermore, in the phosphorescent group, plasma FSH, LH, and estradiol-17ß (E2) levels were significantly higher than in the photoperiod groups. However, plasma melatonin levels were significantly lower than in the photoperiod groups. Because LumiNova sheets continue to emit green light (520 nm) for approximately 2h after sunset, the extended light conditions probably contributed to reproductive ability in the experimental fish. In conclusion, long-afterglow phosphorescent pigment can be used for energy-efficient aquaculture to regulate the reproduction of fish, although its effect needs to be evaluated in other species.
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Substâncias Luminescentes/farmacologia , Ovário/fisiologia , Perciformes/fisiologia , Animais , Estradiol/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Regulação da Expressão Gênica no Desenvolvimento , Gonadotropinas/genética , Hormônio Luteinizante/sangue , Hormônio Luteinizante/genética , Masculino , Melatonina/metabolismo , Ovário/efeitos dos fármacos , Fotoperíodo , Receptores de Estrogênio/genética , Reprodução/efeitos dos fármacos , Reprodução/fisiologia , Maturidade Sexual , Vitelogeninas/genéticaRESUMO
A low-loss 4-ch active optical interconnect (AOI) enabling passive alignment was proposed and built resorting to a transmitter (Tx) incorporating a red 680-nm VCSEL, which is linked to a receiver (Rx) module via a partially chlorinated ribbon POF. The POF was observed to exhibit an extremely low loss of ~0.24 dB/m at λ = 680 nm, in comparison to ~1.29 dB/m at λ = 850 nm, and a large numerical aperture of ~0.42. Both the Tx and Rx, which taps into a beam router based on collimated beam optics involving a pair of spherical lenses, were meant to be substantially alignment tolerant and compact. The achieved tolerance for the constructed modules was beyond 40 µm in terms of the positioning of VCSEL and photodetector. The proposed AOI was completed by linking the Tx with the Rx via a 3-m long ribbon POF, incurring a transmission loss of as small as 3.2 dB. The AOI was practically assessed in terms of a high-speed data transmission over a wide range of temperatures and then exploited to convey full HD video signals.
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Peroxiredoxins (Prxs), also known as natural killer cell enhancing factors in fish, role as antioxidant proteins and participate in a variety of biological processes, including H2O2-mediated cell signaling, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 1 cDNA from the Korean rose bitterling Rhodeus uyekii, and designated it RuPrx 1. The RuPrx 1 cDNA encodes a 197-amino-acid polypeptide that belongs to the class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced RuPrx 1 protein shows strong homology (77.38-92.89 %) with Prx 1 proteins from other species, including fish, amphibians, and mammals, and it is most closely related to rainbow smelt Prx 1. RuPrx 1 mRNA was ubiquitously detected in all tested tissues and its expression was comparatively high in the brain, intestine, kidney, liver, ovary, stomach, and testis. Expression of RuPrx 1 mRNA in liver peaked 3 h post-infection with Aeromonas hydrophila and decreased 24 h post-infection while the expression in intestine decreased 24 h post-infection. These results suggest that RuPrx 1 is conserved through evolution and may play roles similar to its mammalian counterparts.
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Cyprinidae/genética , DNA Complementar , Expressão Gênica , Peroxirredoxinas/genética , Aeromonas hydrophila , Sequência de Aminoácidos , Animais , Sequência de Bases , Cyprinidae/classificação , DNA Complementar/química , Doenças dos Peixes/genética , Doenças dos Peixes/microbiologia , Perfilação da Expressão Gênica , Dados de Sequência Molecular , Especificidade de Órgãos/genética , Peroxirredoxinas/química , Filogenia , RNA Mensageiro/genética , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
We found a rare muscular variation in the superficial region of the popliteal fossa in a 61-year-old Korean male cadaver whose cause of death was laryngeal carcinoma during routine dissection course for medical students. The muscle ran transversely between the medial head of the gastrocnemius muscle and the tendon of the long head of biceps femoris muscle, covering the neurovascular structures in the popliteal fossa. The muscle received its nerve supply from the tibial nerve. Based on its innervation, we speculated that the anomalous muscle might be a very specific type of variation related to the gastrocnemius tertius rather than another superficial muscle in the popliteal fossa.
Assuntos
Perna (Membro) , Músculo Esquelético/anormalidades , Cadáver , Dissecação , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Esquelético/irrigação sanguínea , Músculo Esquelético/inervação , República da CoreiaRESUMO
We identified and characterized the primary structure of the Korean oily bitterling Acheilognathus koreensis fast skeletal myosin light chain 2 (Akmlc2f), gene. Encoded by seven exons spanning 3955 bp, the deduced 168-amino acid AkMLC2f polypeptide contained an EF-hand calcium-binding motif and showed strong homology (80%-98%) with the MLC2 proteins of Ictalurus punctatus and other species, including mammals. Akmlc2f mRNA was highly enriched in skeletal muscles, and was detectable in other tissues. The upstream regions of Akmlc2f included a TATA box, one copy of a putative MEF-2 binding site and several putative C/EBPß binding sites. The functional activity of the promoter region of Akmlc2f was examined using luciferase and red fluorescent protein reporters. The Akmlc2f promoter-driven reporter expressions were detected and increased by the C/EBPß transcription factor in HEK293T cells. The activity of the promoter of Akmlc2f was also confirmed in the developing zebrafish embryo. Although the detailed mechanism underlying the expression of Akmlc2f remains unknown, these results suggest the muscle-specific expression of Akmlc2f transcript and the functional activation of Akmlc2f promoter by C/EBPß.
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Miosinas Cardíacas/genética , Cyprinidae/genética , Músculo Esquelético/metabolismo , Cadeias Leves de Miosina/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação/genética , Proteína beta Intensificadora de Ligação a CCAAT/metabolismo , Cálcio/metabolismo , Linhagem Celular , DNA/análise , Expressão Gênica , Células HEK293 , Humanos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , RNA Mensageiro/análise , República da Coreia , Alinhamento de Sequência , TATA Box/genética , Peixe-Zebra/embriologia , Peixe-Zebra/genéticaRESUMO
The study examined the comparative assessment of destination image according to the perception of COVID-19 and travel risk among international students. The online survey was administered to 786 international students enrolled in universities in Korea. Cluster analysis was performed, and three distinct clusters were identified based on risk perception. Destination image attributes were generated into four underlying dimensions: social environment, tourist environment, destination environment, and imagery, using the exploratory factor analysis. A revised Importance-Performance Analysis (IPA) method was utilized to assess the destination image of Korea and used to compare the expected performance of the attributes on each cluster. In addition, a revised IPA grid of each cluster was presented to unveil the satisfaction attributes of the destination image and suggest communication strategies. This study provides practical insights to destination marketers and organizations to design marketing strategies for international students. Further practical and theoretical implications are discussed.
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Two paralogous isoform cDNAs of warm-temperature-acclimation-associated 65-kDa protein (Wap65-1 and Wap65-2) were isolated from the cypriniform species, mud loach (Misgurnus mizolepis), and characterized. The deduced amino acid sequences of the two mud loach Wap65 isoforms (mlWap65-1 and mlWap65-2) share moderate levels of sequence homology with their corresponding orthologues from teleosts and with human hemopexin, a possible mammalian homologue. Both isoforms display conserved features, including essential motifs and/or residues that are important for the protein structure of hemopexin. In overall, mlWap65-2 is more homologous to human hemopexin than is mlWap65-1. Both mud loach Wap65 transcripts are predominantly expressed in liver, although the transcripts are ubiquitously detectable in most tissues with variable basal expression. Both mlWap65 isoforms are differentially regulated during embryonic development, and the changes in transcript levels during embryogenesis are greater for mlWap65-2 than for mlWap65-1. The transcription of the mlWap65 genes is differentially modulated by various stimuli, including thermal changes, immune challenge (lipopolysaccharide injection or bacterial infection), and heavy metal exposure (cadmium, copper, or nickel). The isoform mlWap65-1 is more responsive to warm temperature treatments than mlWap65-2, whereas mlWap65-2 is much more strongly stimulated by immune and heavy metal challenges than is mlWap65-1. Taken together, the results of this study suggest that mud loach Wap65 isoforms are potentially involved in multiple cellular pathways and that the two mud loach Wap65 isoforms undergo functional partitioning or subfunctionalization.
Assuntos
Cipriniformes/genética , Proteínas de Peixes/metabolismo , Hemopexina/análogos & derivados , Hemopexina/metabolismo , Fígado/metabolismo , Aclimatação , Sequência de Aminoácidos , Animais , Cipriniformes/metabolismo , DNA Complementar/química , DNA Complementar/genética , DNA Complementar/metabolismo , Proteínas de Peixes/química , Proteínas de Peixes/genética , Hemopexina/química , Hemopexina/genética , Temperatura Alta , Dados de Sequência Molecular , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Reação em Cadeia da Polimerase em Tempo Real/veterináriaRESUMO
Galectin-9 is a b-galactoside-binding lectin that regulates many cellular functions, ranging from cell adhesion to pathogen recognition. We isolated and characterized the cDNA of tandem-repeat galectin-9 (RuGlec9) from the Korean rose bitterling (Rhodeus uyekii), an endemic Korean fish belonging to the Acheilognathinae subfamily of the Cyprinidae family. RuGlec9 cDNA is 1486 bp long and encodes a polypeptide of 323 amino acids containing two carbohydrate-recognition domains connected by a linker peptide. The deduced amino acid sequence of RuGlec9 shows 45-84% amino acid sequence identity to other galectin-9 sequences, including those from mammals and fish. RuGlec9 appeared in a large cluster with other galectin-9 sequences from fish and is more closely related to galectin-9 from Danio rerio than to those of other fish and mammals. RuGlec9 mRNA was expressed highly in the testis, spleen, intestine, stomach, and liver, and moderately in the brain, kidney, ovary, and gills of normal Korean rose bitterling. RuGlec9 mRNA expression in the spleen was increased by lipopolysaccharide. These results suggest that RuGlec9 plays a role in innate immunity in Korean rose bitterling.