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1.
Avian Dis ; 59(4): 548-53, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26629631

RESUMO

Single swabs (cultured individually) are currently used in the Food and Drug Administration (FDA) official method for sampling the environment of commercial laying hens for the detection of Salmonella enterica ssp. serovar Enteritidis (Salmonella Enteritidis). The FDA has also granted provisional acceptance of the National Poultry Improvement Plan's (NPIP) Salmonella isolation and identification methodology for samples taken from table-egg layer flock environments. The NPIP method, as with the FDA method, requires single-swab culturing for the environmental sampling of laying houses for Salmonella Enteritidis. The FDA culture protocol requires a multistep culture enrichment broth, and it is more labor intensive than the NPIP culture protocol, which requires a single enrichment broth. The main objective of this study was to compare the FDA single-swab culturing protocol with that of the NPIP culturing protocol but using a four-swab pool scheme. Single and multi-laboratory testing of replicate manure drag swab sets (n  =  525 and 672, respectively) collected from a Salmonella Enteritidis-free commercial poultry flock was performed by artificially contaminating swabs with either Salmonella Enteritidis phage type 4, 8, or 13a at one of two inoculation levels: low, x¯  = 2.5 CFU (range 2.5-2.7), or medium, x¯  = 10.0 CFU (range 7.5-12). For each replicate, a single swab (inoculated), sets of two swabs (one inoculated and one uninoculated), and sets of four swabs (one inoculated and three uninoculated), testing was conducted using the FDA or NPIP culture method. For swabs inoculated with phage type 8, the NPIP method was more efficient (P < 0.05) for all swab sets at both inoculation levels than the reference method. The single swabs in the NPIP method were significantly (P < 0.05) better than four-pool swabs in detecting Salmonella Enteritidis at the lower inoculation level. In the collaborative study (n  =  13 labs) using Salmonella Enteritidis phage type 13a inoculated swabs, there was no significant difference (P > 0.05) between the FDA method (single swabs) and the pooled NPIP method (four-pool swabs). The study concludes that the pooled NPIP method is not significantly different from the FDA method for the detection of Salmonella Enteritidis in drag swabs in commercial poultry laying houses. Consequently based on the FDA's Salmonella Enteritidis rule for equivalency of different methods, the pooled NPIP method should be considered equivalent. Furthermore, the pooled NPIP method was more efficient and cost effective.


Assuntos
Criação de Animais Domésticos/métodos , Técnicas Bacteriológicas/veterinária , Galinhas , Doenças das Aves Domésticas/diagnóstico , Salmonelose Animal/diagnóstico , Salmonella enteritidis/isolamento & purificação , Manejo de Espécimes/veterinária , Animais , Técnicas Bacteriológicas/instrumentação , Esterco/microbiologia , Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/microbiologia , Sensibilidade e Especificidade , Manejo de Espécimes/instrumentação
2.
J Vet Diagn Invest ; 32(6): 942-947, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33140708

RESUMO

Two horses that consumed well water with high fluoride content exhibited clinical signs of chronic dental and skeletal fluoride toxicosis and were later euthanized and autopsied. Both horses had degenerative disease of multiple joints and multiple dental defects. Elevated fluoride concentrations were found in bone and tooth samples of both horses, well water, and feed. Microscopically, abnormalities were noted in bone and tooth samples, and consisted mostly of foci of cement necrosis and hypercementosis. Horses exhibiting bilateral, highly symmetrical dental and/or skeletal lesions, with chronic lameness, should be evaluated for the possible presence of fluoride toxicosis.


Assuntos
Osso e Ossos/efeitos dos fármacos , Água Potável/química , Fluoretos/toxicidade , Fluorose Dentária/veterinária , Doenças dos Cavalos/induzido quimicamente , Animais , Osso e Ossos/química , Fluoretos/análise , Fluorose Dentária/etiologia , Cavalos
3.
Vet Microbiol ; 136(3-4): 209-16, 2009 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-19059738

RESUMO

Over a three-year period, 2004-2007, greater than 12,000 alpacas in the United States were screened by real-time RT-PCR to identify alpacas persistently infected (PI) with bovine viral diarrhea virus (BVDV). A total of 46 BVD viruses were isolated from PI alpacas or diagnostic samples from alpacas. Forty-three US alpaca BVDV isolates and 3 Canadian isolates were analyzed by comparison of nucleotide sequences of two viral genomic regions, the 5'-UTR and the N(pro) gene to determine their genetic relatedness. All 46 alpaca BVDV isolates from 8 different states of the US and Canada were genotype 1b with > or =99% nt identity in the 290-base 5'-UTR region with the exception of one Canadian isolate. In contrast, 21 bovine BVDV isolates collected during the same period were grouped into the typical 3 genotypes, 1a, 1b, and 2, respectively. Forty five alpaca BVDV isolates formed a distinctive cluster separated from closely related bovine genotype 1b isolates by phylogenetic analysis of the 5'-UTR region. Comparison of the 504-base N(pro) gene sequences of 32 alpaca isolates also assigned them all to type 1b in a similar fashion as observed with the 5'-UTR region. The results suggest that unique genotypes of bovine BVDV 1b may be maintained in the alpaca population even though camelids are susceptible to infection by other genotypes. Further studies are needed to address why alpacas were predominantly infected with genotype 1b BVDV isolates and how bovine BVD viruses evolved to infect alpacas.


Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Camelídeos Americanos/virologia , Portador Sadio/veterinária , Vírus da Diarreia Viral Bovina/genética , Regiões 5' não Traduzidas , Animais , Sequência de Bases , Doença das Mucosas por Vírus da Diarreia Viral Bovina/epidemiologia , Portador Sadio/epidemiologia , Portador Sadio/virologia , Bovinos , Vírus da Diarreia Viral Bovina/isolamento & purificação , Genótipo , Dados de Sequência Molecular , América do Norte/epidemiologia , Filogenia , Prevalência , RNA Viral/química , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Alinhamento de Sequência , Proteínas Virais/química , Proteínas Virais/genética
4.
J Food Prot ; 70(1): 47-52, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17265859

RESUMO

A joint multiagency project was initiated in response to a Salmonella outbreak in San Diego County, California, in 2004. Samples of cheese were collected during four 1-day operations at the San Ysidro port of entry, along the United States-Mexico border. Surveyed participants were persons crossing the border as pedestrians or in vehicles who had a minimum of 2.27 kg of cheese, which may suggest a potential diversion to illegal marketing. In addition, data were collected about the cheese to identify risk factors for cheese contamination. Two hundred four cheese samples were submitted to the California Animal Health and Food Safety Laboratory System-San Bernardino Branch and analyzed for potential food pathogens. Ninety-four percent (190 of 203) of the samples tested positive for alkaline phosphatase. Salmonella was detected from 13% (27 of 204) of the samples comprising 11 serogroups and 28 serotypes. Pulsed-field gel electrophoresis DNA fingerprinting analysis, performed following standardized methods, determined that an isolate obtained from this study had an indistinguishable pattern from a recent Salmonella enterica serovar Typhimurium var. Copenhagen epidemic in the San Diego County that was linked to 14 illnesses. Listeria spp. were detected from 4% (8 of 204) of the samples, and of these, half were identified as L. monocytogenes. Escherichia coli O157:H7 was not detected from any of the samples. Mycobacterium bovis was detected from one panela-style cheese sample. Nine additional samples yielded Mycobacterium spp.


Assuntos
Queijo/microbiologia , Comércio , Contaminação de Alimentos/análise , Listeria monocytogenes/isolamento & purificação , Mycobacterium bovis/isolamento & purificação , Salmonella/isolamento & purificação , Fosfatase Alcalina/metabolismo , California/epidemiologia , Surtos de Doenças , Microbiologia de Alimentos , Humanos , Estados Unidos
5.
J Vet Diagn Invest ; 19(3): 317-22, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17459867

RESUMO

This article describes amebic infections in 4 horses: granulomatous amebic encephalitis caused by Balamuthia mandrillaris and Acanthamoeba culbertsoni and systemic infections caused by Acanthamoeba sp. The former infection occurred in 1 of 4 horses spontaneously without any underlying conditions; the latter amebic infection was perhaps "opportunistic" considering the visceral involvement by this protozoan in association with Aspergillus sp. and/or Escherichia coli and Pseudomonas sp. The clinicopathologic findings and demonstration of the amebic organisms using immunohistochemical techniques, culture, polymerase chain reactions, and electron microscopy are presented.


Assuntos
Acanthamoeba/isolamento & purificação , Amebíase/veterinária , Encefalopatias/veterinária , Doenças dos Cavalos/parasitologia , Pneumopatias Parasitárias/veterinária , Acanthamoeba/genética , Amebíase/parasitologia , Animais , Encefalopatias/parasitologia , DNA de Protozoário/química , DNA de Protozoário/genética , Evolução Fatal , Feminino , Cavalos , Imuno-Histoquímica/veterinária , Pneumopatias Parasitárias/parasitologia , Masculino , Reação em Cadeia da Polimerase/veterinária
6.
J Vet Diagn Invest ; 18(3): 296-9, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16789722

RESUMO

The use of sentinel chickens in establishing the negative status of commercial poultry flocks depopulated due to exotic Newcastle disease (END) is considered to be an economically beneficial process. However, the costs and benefits of using sentinel chickens in noncommercial operations are in question. The objective of this study was to use sentinel chickens to evaluate whether adequate cleaning and disinfection coupled with an appropriate time period without susceptible poultry species on the premises would eliminate END virus from a noncommercial poultry operation and preclude the need for placement of sentinels in previously infected operations before declaring them free of virus. Noncommercial poultry operations were selected from the 2002 to 2003 END outbreak database. Operations included in the study had one or more isolations of END virus (ENDV) from cloacal or oropharyngeal swabs of birds on the premises. A total of 546 birds were placed on 53 premises. All sentinel birds sampled after placements were negative by virus detection methods and serologic tests. Results of this study indicate that time and the application of appropriate cleaning and disinfection procedures will adequately mitigate the risk of viable virus persisting in noncommercial poultry operations. In the future, this information may eliminate the need for sentinel bird placement to ensure virus free status of premises before repopulation, thereby decreasing the costs of END eradication.


Assuntos
Galinhas , Surtos de Doenças/veterinária , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/crescimento & desenvolvimento , Doenças das Aves Domésticas/virologia , Vigilância de Evento Sentinela/veterinária , Animais , Anticorpos Antivirais/sangue , California/epidemiologia , Embrião de Galinha , Surtos de Doenças/prevenção & controle , Desinfecção/normas , Testes de Hemaglutinação/veterinária , Doença de Newcastle/economia , Doença de Newcastle/epidemiologia , Doença de Newcastle/prevenção & controle , Vírus da Doença de Newcastle/genética , Doenças das Aves Domésticas/economia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/prevenção & controle , RNA Viral/química , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Organismos Livres de Patógenos Específicos
7.
Am J Vet Res ; 67(5): 858-68, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16649922

RESUMO

OBJECTIVE: To determine the distribution for limbs and bones in horses with fractures of the proximal sesamoid bones and relationships with findings on palmarodorsal radiographic images. SAMPLE POPULATION: Proximal sesamoid bones obtained from both forelimbs of cadavers of 328 racing Thoroughbreds. PROCEDURE: Osteophytes; large vascular channels; and fracture location, orientation, configuration, and margin distinctness were categorized by use of high-detail contact palmarodorsal radiographs. Distributions of findings were determined. Relationships between radiographic findings and fracture characteristics were examined by use of chi2 and logistic regression techniques. RESULTS: Fractures were detected in 136 (41.5%) horses. Biaxial fractures were evident in 109 (80%) horses with a fracture. Osteophytes and large vascular channels were evident in 266 (81%) and 325 (99%) horses, respectively. Medial bones typically had complete transverse or split transverse simple fractures, indistinct fracture margins, > 1 vascular channel that was > 1 mm in width, and osteophytes in abaxial wing and basilar middle or basilar abaxial locations. Lateral bones typically had an oblique fracture and distinct fracture margins. Odds of proximal sesamoid bone fracture were approximately 2 to 5 times higher in bones without radiographic evidence of osteophytes or large vascular channels, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Biaxial fractures of proximal sesamoid bones were common in cadavers of racing Thoroughbreds. Differences between medial and lateral bones for characteristics associated with fracture may relate to differences in fracture pathogeneses for these bones. Osteophytes and vascular channels were common findings; however, fractures were less likely to occur in bones with these features.


Assuntos
Membro Anterior/diagnóstico por imagem , Membro Anterior/lesões , Fraturas Ósseas/veterinária , Doenças dos Cavalos/diagnóstico por imagem , Ossos Sesamoides/diagnóstico por imagem , Ossos Sesamoides/lesões , Distribuição por Idade , Animais , Cadáver , Feminino , Fraturas Ósseas/diagnóstico por imagem , Cavalos , Masculino , Radiografia , Distribuição por Sexo
8.
J Vet Diagn Invest ; 17(3): 252-5, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15945382

RESUMO

A 2-year-old bay Thoroughbred colt was found dead overnight in its stall without a known history of any illness, existing disease, or toxicant exposure. No information on the clinical signs before this animal's death was reported. A full necropsy was performed the next morning and revealed a mild to moderate degree of endocardial hemorrhages in both ventricles. Microscopic examination of the heart showed an acute mild multifocal necrosis of papillary muscles and ventricles. The stomach content contained approximately 2% Taxus alkaloids as determined by gas chromatography/mass spectrometry. In the past, diagnosis of Taxus poisoning has been mainly based on history of exposure and the presence of plant parts in the gastrointestinal tract. Pathological lesions associated with Taxus poisoning have not been published for horses. Therefore, this is the first report of cardiac lesions in a horse after lethal exposure to Taxus. On the basis of these findings, it is suggested that Taxus exposure needs to be considered in the differential diagnosis of horses that die suddenly or have cardiac lesions suggestive of Taxus exposure, even if intact plant parts are not identified in the stomach by the naked eye.


Assuntos
Doenças dos Cavalos/diagnóstico , Intoxicação por Plantas/veterinária , Taxus/intoxicação , Alcaloides/análise , Animais , Cromatografia Gasosa-Espectrometria de Massas , Doenças dos Cavalos/etiologia , Doenças dos Cavalos/patologia , Cavalos , Masculino , Miocárdio/patologia , Intoxicação por Plantas/diagnóstico , Intoxicação por Plantas/etiologia , Intoxicação por Plantas/patologia
9.
J Vet Diagn Invest ; 17(2): 198-200, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15825506

RESUMO

The 2002--2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.


Assuntos
Doença de Newcastle/diagnóstico , Vírus da Doença de Newcastle/isolamento & purificação , Animais , California/epidemiologia , Surtos de Doenças/veterinária , Doença de Newcastle/epidemiologia , Vírus da Doença de Newcastle/genética , Aves Domésticas , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
10.
J Vet Diagn Invest ; 17(6): 605-9, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16475525

RESUMO

Primary amoebic meningoencephalitis is a fulminant infection of the human central nervous system caused by Naegleria fowleri, a free-living amoeba that thrives in artificially or naturally heated water. The infection usually is acquired while bathing or swimming in such waters. The portal of entry is the olfactory neuroepithelium. This report describes fatal meningoencephalitis caused by N. fowleri in Holstein cattle that consumed untreated surface water in an area of California where summer temperatures at times exceed 42 degrees C. In the summers of 1998 and 1999, severe multifocal necrosuppurative hemorrhagic meningoencephalitis was observed in brain samples from nine 10-20-month-old heifers with clinical histories of acute central nervous system disease. Olfactory lobes and cerebella were most severely affected. Lesions were also evident in periventricular and submeningeal neuropil as well as olfactory nerves. Naegleria fowleri was demonstrated by immunohistochemistry in brain and olfactory nerve lesions and was isolated from one brain. Even though cultures of drinking water did not yield N. fowleri, drinking water was the likely source of the amoeba. The disease in cattle closely resembles primary amoebic meningoencephalitis in humans. Naegleria meningoencephalitis should be included among differential diagnoses of central nervous system disease in cattle during the summer season in areas with high ambient temperatures.


Assuntos
Amebíase/parasitologia , Amebíase/veterinária , Doenças dos Bovinos/parasitologia , Meningoencefalite/parasitologia , Meningoencefalite/veterinária , Naegleria fowleri/isolamento & purificação , Estações do Ano , Animais , Encéfalo/parasitologia , Encéfalo/patologia , Bovinos , Surtos de Doenças/veterinária
11.
Avian Dis ; 49(2): 189-94, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16094821

RESUMO

Between August 20, 2001, and September 17, 2002, 1429 samples including drag swabs, egg belt or egg rollout swabs, fan-blade swabs, rodent organ and intestinal pools, beetle (Alphitobius diaperinus) pools, housefly (Musca domestica) pools, chicken organ and intestinal pools, and egg pools were obtained for Salmonella culture from two flocks from two different commercial layer ranches. The two ranches were purposefully selected for the study based on their previous status of Salmonella Enteritidis isolation using environmental drag swabs in cooperation with practicing veterinarians. Salmonella sp. was isolated from 337 out of 979 (34.42%) non-egg samples. No Salmonella was isolated from 450 egg pools collected from either ranch. S. enteritidis was isolated from samples obtained from ranch 1 from manure drag swabs, 4/284 (1.4%); rodent organs, 1/24 (4.2%); and housefly pool cultures 1/21 (4.8%). Salmonella Enteritidis was isolated from ranch 2 from mouse organ and intestinal pool samples, 1/24 (4.2%). Salmonella group B was isolated from all sample types except the insects. There was a statistically significant difference in isolation rates among seven serogroups of Salmonella: groups B, C1, C2, D, E, K, and untypeable (Pearson chi-square 18.96, P = 0.002). Overall, statistically significant differences were observed with respect to Salmonella isolation among the types of samples taken (Pearson chi-square 118.54, P < 0.0001). Intensive monitoring for Salmonella Enteritidis can be used to optimize a Salmonella reduction program for an individual poultry biosecurity unit.


Assuntos
Galinhas , Microbiologia Ambiental , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/diagnóstico , Salmonella enteritidis/isolamento & purificação , Animais , California/epidemiologia , Ovos/virologia , Fezes/virologia , Abrigo para Animais , Insetos/virologia , Doenças das Aves Domésticas/epidemiologia , Salmonelose Animal/epidemiologia , Especificidade da Espécie , Vísceras/virologia
12.
J Vet Diagn Invest ; 27(1): 112-6, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25525145

RESUMO

Five horses originating from 4 different California race tracks were submitted to the California Animal Health and Food Safety Laboratory for necropsy and diagnostic workup. The 5 horses had a history of sudden collapse and death during exercise. In all of them, massive hemoperitoneum and hemorrhages in other cavities or organs were observed. The liver from these 5 animals and from 27 horses that had been euthanized due to catastrophic leg injuries (controls) were subjected to a rodenticide anticoagulant screen. Traces of brodifacoum, diphacinone, or bromadiolone were detected in the 5 horses with massive bleeding (5/5), and no traces of rodenticides were detected in control horses (0/27). Other frequent causes of massive hemorrhages in horses were ruled out in 4 of the cases; one of the horses had a pelvic fracture. Although only traces of anticoagulants were found in the livers of these horses and the role of these substances in the massive bleeding remains uncertain, it is speculated that exercise-related increases in blood pressure may have reduced the threshold for toxicity of these anticoagulants.


Assuntos
Anticoagulantes/toxicidade , Hemorragia/veterinária , Doenças dos Cavalos/induzido quimicamente , Condicionamento Físico Animal , Rodenticidas/toxicidade , 4-Hidroxicumarinas/toxicidade , Animais , California , Hemoperitônio/induzido quimicamente , Hemoperitônio/veterinária , Hemorragia/induzido quimicamente , Cavalos , Fígado/química , Masculino , Fenindiona/análogos & derivados , Fenindiona/toxicidade
13.
Int J Parasitol ; 33(10): 1115-25, 2003 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-13129534

RESUMO

Phylogenetic relationships among six isolates of Halicephalobus gingivalis (Stefanski, 1954), a species with pathogenic potential in horses and humans, were evaluated using DNA sequences from the nuclear large-subunit ribosomal RNA (LSU rDNA) gene. Sequences from nematodes obtained from in vitro cultures (soil or clinical sources), or isolated from infected horse tissues, were compared. Gene sequences from a fatal equine clinical case from southern California and a free-living isolate recovered from southern California soil showed no fixed differences. Sequences from isolates representing two fatal equine cases from North America, one from Ontario, Canada and another from Tennessee also showed no fixed differences. In contrast, two equine cases from Tennessee had 18 fixed differences for this LSU region, the greatest observed among isolates from horses. Phylogenetic analysis of six Halicephalobus sequences and four outgroup taxa by maximum parsimony yielded one tree with five well-supported clades. This phylogeny did not group isolates of Halicephalobus strictly by region of geographic isolation or source of sample, and depicted one clinical and one soil isolate as sister taxa. These results confirm that free-living environmental isolates are potential sources of infection for horses. The phylogeny also reveals that diverse isolates can cause infections in horses within a relatively limited geographic region, and conversely that genetically similar sister taxa can be recovered from geographically distant localities. PCR primers that selectively amplify Halicephalobus DNA were designed and tested based on comparison of closely related nematodes as inferred from phylogenetic analysis.


Assuntos
Doenças dos Cavalos/diagnóstico , Infecções Oportunistas/veterinária , Infecções por Rhabditida/veterinária , Rabditídios/classificação , Solo/parasitologia , Animais , Sequência de Bases , DNA de Helmintos/genética , DNA Ribossômico/genética , Doenças dos Cavalos/parasitologia , Cavalos , Dados de Sequência Molecular , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/parasitologia , Filogenia , Reação em Cadeia da Polimerase/métodos , Rabditídios/genética , Infecções por Rhabditida/diagnóstico , Infecções por Rhabditida/parasitologia , Alinhamento de Sequência
14.
Avian Dis ; 48(3): 669-74, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15529993

RESUMO

During the first weeks of 2003, after exotic Newcastle disease (END) was confirmed in commercial layer flocks in Southern California, it became apparent that the virus survival information in the literature varied widely and was difficult to extrapolate to current local conditions. The END Task Force used the information available in the literature and the recommendations of research scientists to establish protocols for safely handling manure from infected and depopulated premises. In an attempt to gain more applicable knowledge in the management of contaminated poultry manure in the course of the END outbreak, this virus survival study was designed and implemented. Environmental drag swabs were tested for END virus from two of the early-infected commercial ranches that consisted of several houses following immediate removal of the infected flocks. A total of 293 samples, composed of 168 manure drag swab pools, 72 dropping board swab pools, and 38 compost swab pools from 3 houses (ranch 1), and 180 manure belt scraper swab pools from ranch 2 were analyzed for ND virus isolation and characterization for 21 consecutive days postdepopulation. Thirteen manure drag swab pools (from houses 1 and 3) and two manure dropping board swab pools (from house 3) collected from ranch 1 were positive for END virus at 0, 1, 2, 3, 4, 7, 10, 12, and 16 days postdepopulation. No END virus was isolated after the 16th day following depopulation from any of the samples. All samples from ranch 2 were negative during the entire observation period.


Assuntos
Galinhas/virologia , Fezes/microbiologia , Abrigo para Animais , Vírus da Doença de Newcastle/isolamento & purificação , Animais , California , Vírus da Doença de Newcastle/crescimento & desenvolvimento , Análise de Sobrevida , Temperatura , Fatores de Tempo
15.
Avian Dis ; 47(3 Suppl): 1214-8, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14575145

RESUMO

Between February 2000 and February 2002, the California Animal Health and Food Safety Laboratory System diagnosed 26 cases of low-pathogenic H6N2 avian influenza from 12 commercial egg-laying farms. The most common gross and histologic lesions observed in infected chickens were fibrinous yolk peritonitis, salpingitis, oophoritis, and nephritis. Edema of the mesentery of the oviduct and pale, swollen kidneys were also observed. Mortality in infected flocks ranged from 0.25% to 3%, and egg production dropped 7% to 40%.


Assuntos
Galinhas/virologia , Vírus da Influenza A/classificação , Vírus da Influenza A/isolamento & purificação , Influenza Aviária/epidemiologia , Oviposição/fisiologia , Animais , California/epidemiologia , Surtos de Doenças/veterinária , Feminino , Influenza Aviária/patologia , Peritonite/veterinária , Peritonite/virologia
16.
Avian Dis ; 48(3): 550-61, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15529977

RESUMO

This cross-sectional, double-blind study reports the prevalence of Salmonella enterica serotype enteritidis (SE) on California egg layer premises using single vs. pooled manure drag swabs and presents a description of egg production and management systems in the state and an initial analysis of risk factors for SE. The study included 91% of all known eligible egg premises in California, representing the majority of eggs produced in the state. The overall prevalence of SE on California egg layer premises was 10.5%, while 1.1% of all rows sampled were positive for SE. The percentage of positive rows for SE on any premises never exceeded 25% of the 16 swabs collected per premises. A description of egg production and management on California egg layer premises is presented. Statistically significant associations for SE were not evident and were limited because of sample size and the low prevalence of SE on California egg layer premises. Several biological and management factors, such as flock health, stage of production, manure management, ventilation, and watering systems, show trend associations with premises positive for SE and require further investigation. Manure drag swabs serve as a useful tool to validate the core components of an egg quality assurance program for SE based on process control principles.


Assuntos
Criação de Animais Domésticos/normas , Galinhas , Ovos , Fezes/microbiologia , Abrigo para Animais , Salmonella enteritidis/isolamento & purificação , Animais , California , Estudos Transversais , Método Duplo-Cego , Microbiologia Ambiental , Estudos Prospectivos , Fatores de Risco
17.
Avian Dis ; 48(3): 590-4, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15529981

RESUMO

Between the summer of 1998 and the winter of 2000, Salmonella analysis was performed on 2128 single and 532 pooled manure drag swabs obtained from 133 California commercial egg laying farms. The isolation of Salmonella from all rows and from all flocks using single or pooled swabs was 80% and 92%, respectively. Hence, there was no statistical difference between single vs. pooled swabs in terms of identifying Salmonella on a row or flock basis. A total of 14 serogroups comprising 44 serotypes were isolated from 123 of 133 farms. When the top 10 serotypes were considered, there was no significant difference in the range of serotypes isolated by the two culturing methods. The overall S. enteritidis prevalence for California flocks was 10.5% (14/133). The overall row prevalence for S. enteritidis for all the farms was 1.1% (24/2128), and the overall pool prevalence was 2.4% (13/532). Sixty percent (12/20) of the S. enteritidis isolates from the positive farms were phage type 4, and 40% (8/20) represented five other phage types (1, 6B, 7, 8, and 28).


Assuntos
Galinhas , Ovos , Fezes/microbiologia , Abrigo para Animais , Salmonella enteritidis/isolamento & purificação , Manejo de Espécimes/métodos , Animais , California , Microbiologia Ambiental , Sorotipagem
18.
J Am Vet Med Assoc ; 221(7): 1007-13, 2002 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-12369679

RESUMO

OBJECTIVE: To determine sensitivity and specificity of western blot testing (WBT) of CSF and serum for diagnosis of equine protozoal myeloencephalitis (EPM) in horses with and without neurologic abnormalities. DESIGN: Prospective investigation. ANIMALS: 65 horses with and 169 horses without neurologic abnormalities. PROCEDURE: CSF and serum from horses submitted for necropsy were tested for Sarcocystis neurona-specific antibody with a WBT. Results of postmortem examination were used as the gold standard against which results of the WBT were compared. RESULTS: Sensitivity of WBT of CSF was 87% for horses with and 88% for horses without neurologic abnormalities. Specificity of WBT of CSF was 44% for horses with and 60% for horses without neurologic abnormalities. Regardless of whether horses did or did not have neurologic abnormalities, sensitivity and specificity of WBT of serum were not significantly different from values for WBT of CSF. Ninety-four horses without EPM had histologic evidence of slight CNS inflammation. CONCLUSIONS AND CLINICAL RELEVANCE: The low specificity of WBT of CSF indicated that it is inappropriate to diagnose EPM on the basis of a positive test result alone because of the possibility of false-positive test results. The high sensitivity, however, means that a negative result is useful in ruling out EPM. There was no advantage in testing CSF versus serum in horses without neurologic abnormalities. Slight CNS inflammation was common in horses with and without S neurona-specific antibodies in the CSF and should not be considered an indication of CNS infection with S neurona.


Assuntos
Western Blotting/veterinária , Encefalomielite/veterinária , Doenças dos Cavalos/diagnóstico , Sarcocystis/imunologia , Sarcocistose/veterinária , Animais , Western Blotting/métodos , Encefalomielite/sangue , Encefalomielite/líquido cefalorraquidiano , Encefalomielite/diagnóstico , Reações Falso-Positivas , Feminino , Doenças dos Cavalos/sangue , Doenças dos Cavalos/líquido cefalorraquidiano , Cavalos , Masculino , Estudos Prospectivos , Sarcocistose/sangue , Sarcocistose/líquido cefalorraquidiano , Sarcocistose/diagnóstico , Sensibilidade e Especificidade
19.
J Vet Diagn Invest ; 26(1): 96-103, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24532694

RESUMO

Sixteen years of adult cattle submissions to the California Animal Health and Food Safety Laboratory System were examined and data captured from cases with anaerobic cultures of intestinal content. Analysis was performed to determine if there were statistical differences between case submission types (nonbloody intestinal content [129 cases], bloody intestinal content [134 cases], and jejunal hematoma [JH; 51 cases]) for the presence of Clostridium perfringens (314 cases), C. perfringens toxinotypes (35 cases), and C. perfringens toxins (51 cases) in the content. Across submission types, significant differences were found in the isolation of C. perfringens between different specimen types (live cow, dead cow, or tissue from a field necropsy) with field samples being the most likely to have C. perfringens detected and live animals the least likely (P = 0.001). In cases of JH, detection of C. perfringens by enzyme-linked immunosorbent assay was more likely when a live or dead animal was submitted (P = 0.023) or when a live animal was submitted (P = 0.019) compared with submission of field necropsy tissues. These differences were not observed when cultures were performed to detect C. perfringens in cases of JH. There were no statistical differences between submission types with regard to any other variables evaluated. Detailed histologic examination of 21 cases of JH suggested disturbance of normal vascular or lymphatic function as the underlying problem in this entity.


Assuntos
Doenças dos Bovinos/microbiologia , Infecções por Clostridium/veterinária , Clostridium perfringens/isolamento & purificação , Hematoma/veterinária , Doenças do Jejuno/veterinária , Animais , Toxinas Bacterianas/análise , California/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/patologia , Infecções por Clostridium/epidemiologia , Infecções por Clostridium/microbiologia , Infecções por Clostridium/patologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Conteúdo Gastrointestinal/química , Conteúdo Gastrointestinal/microbiologia , Hematoma/epidemiologia , Hematoma/microbiologia , Hematoma/patologia , Histocitoquímica/veterinária , Doenças do Jejuno/epidemiologia , Doenças do Jejuno/microbiologia , Doenças do Jejuno/patologia , Estudos Retrospectivos
20.
J Vet Diagn Invest ; 26(1): 173-7, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24532697

RESUMO

Between 1999 and 2012, 11 cases of congenital portosystemic shunts (cPSS) resulting in hepatic encephalopathy were diagnosed in goat kids necropsied at the California Animal Health and Food Safety Laboratory System and at the Department of Pathology, Immunology & Microbiology, School of Veterinary Medicine, University of California-Davis. Affected animals included 6 females and 5 males of various breeds including Boer (5/11), Nigerian Dwarf (1/11), Saanen (1/11), Toggenburg (1/11), and mixed-breed (3/11) aged between 1.5 months and 11 months, submitted live (2/11) or dead (9/11) for necropsy. The most frequent clinical signs in these goats were ataxia, blindness, tremors, head bobbing, head pressing, seizures, circling, weakness, and ill thrift. Bile acids were measured in 2 animals, and were elevated in both cases (134 and 209 µmol/l, reference interval = 0-50 µmol/l). Necropsy findings were poor to fair body condition. Grossly, the livers of 4 animals were subjectively small. Microscopic lesions included portal spaces with increased numbers of arteriolar profiles and hypoplastic or absent portal veins, diffuse atrophy of the hepatic parenchyma with the presence of small hepatocytes and, in some cases, multifocal hepatocellular macrovesicular vacuolation. In the brain and spinal cord of all animals, there was bilateral and symmetric spongy degeneration affecting the cerebrum, mesencephalon, cerebellum, brainstem, and cervical spinal cord. In all cases, the brain lesions were consistent with hepatic encephalopathy. Congenital portosystemic shunts should be considered in the differential diagnosis of young goats with a history of ill thrift, and nonspecific neurological signs.


Assuntos
Doenças das Cabras/congênito , Encefalopatia Hepática/veterinária , Veia Porta/patologia , Animais , California , Evolução Fatal , Feminino , Doenças das Cabras/patologia , Cabras , Encefalopatia Hepática/congênito , Encefalopatia Hepática/patologia , Histocitoquímica/veterinária , Masculino
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