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1.
J Evid Based Dent Pract ; 21(1): 101532, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-34051959

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Effects of rapid maxillary expansion on temporomandibular joints: A systematic review. Torres D, Lopes J, Magno MB, Cople Maia L, Normando D, Leão PB. Angle Orthod. 2020 Feb 10. https://doi.org/10.2319/080619-517.1. Epub ahead of print. PMID: 32039632. SOURCE OF FUNDING: None. The authors have no actual or potential conflicts of interest. TYPE OF STUDY/DESIGN: Systematic review.


Assuntos
Técnica de Expansão Palatina , Articulação Temporomandibular , Osso e Ossos , Cabeça , Humanos
2.
Hum Factors ; 61(4): 614-626, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30601683

RESUMO

OBJECTIVE: To understand the influence of trust on use choice in human-robot interaction via experimental investigation. BACKGROUND: The general assumption that trusting a robot leads to using that robot has been previously identified, often by asking participants to choose between manually completing a task or using an automated aid. Our work further evaluates the relationship between trust and use choice and examines factors impacting choice. METHOD: An experiment was conducted wherein participants rated a robot on a trust scale, then made decisions about whether to use that robotic agent or a human agent to complete a task. Participants provided explicit reasoning for their choices. RESULTS: While we found statistical support for the "trust leads to use" relationship, qualitative results indicate other factors are important as well. CONCLUSION: Results indicated that while trust leads to use, use is also heavily influenced by the specific task at hand. Users more often chose a robot for a dangerous task where loss of life is likely, citing safety as their primary concern. Conversely, users chose humans for the mundane warehouse task, mainly citing financial reasons, specifically fear of job and income loss for the human worker. APPLICATION: Understanding the factors driving use choice is key to appropriate interaction in the field of human-robot teaming.


Assuntos
Tomada de Decisões , Sistemas Homem-Máquina , Robótica , Confiança , Adolescente , Feminino , Humanos , Masculino , Inquéritos e Questionários , Gravação em Vídeo , Adulto Jovem
3.
J Stud Alcohol Drugs ; 2024 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-38662513

RESUMO

OBJECTIVE: Strong positive links exist between mood, alcohol craving, and sweet taste preference. Research suggests a substitution effect of alcohol with sweets. During alcohol abstinence, individuals have increased craving for alcohol and sweets, in association with anxiety and depression symptoms. Thus, the present study examined the moderating role of sweet taste preference on links between anxiety and depression symptoms and alcohol craving. METHOD: Participants were individuals (N = 91) with alcohol use disorder (AUD) enrolled in a partial hospitalization program. Participants completed baseline assessments of anxiety and depression symptoms, alcohol use and craving, and sweet taste preference. RESULTS: We found significant correlations between symptoms of depression, anxiety, alcohol craving, and sweet taste preference. Sweet taste preference moderated links between both depression and anxiety symptoms with alcohol craving. CONCLUSIONS: For those low and moderate in sweet taste preference, internalizing symptoms appeared positively linked with alcohol craving. For those high in sweet taste preference, alcohol craving remained elevated regardless of anxiety symptoms, but appeared to decrease with heightened depressive symptoms. Should future research replicate this finding using controlled research designs that demonstrate temporality and causality, tailored early AUD interventions may be justified based on individuals' levels of sweet taste preference.

4.
Contemp Clin Trials ; 137: 107411, 2024 02.
Artigo em Inglês | MEDLINE | ID: mdl-38103784

RESUMO

BACKGROUND: Chronic pain affects up to half of individuals taking opioid agonist therapy (OAT; i.e., methadone and buprenorphine) for opioid use disorder (OUD), and yoga-based interventions may be useful for decreasing pain-related disability. Whereas more yoga practice (i.e., higher "dosage") may improve pain-related outcomes, it can be challenging for people with chronic pain taking OAT to attend class regularly and sustain a regular personal yoga practice. Therefore, we plan to optimize a yoga-based intervention (YBI) package in order to support class attendance and personal practice, thus maximizing the yoga dose received. STUDY DESIGN: Using the Multiphase Optimization Strategy (MOST) framework, we will conduct a factorial experiment to examine four intervention components that may be added to a weekly yoga class as part of a YBI. Components include: 1) personal practice videos featuring study yoga teachers, 2) two private sessions with a yoga teacher, 3) daily text messages to inspire personal practice, and 4) monetary incentives for class attendance. The primary outcome will be minutes per week engaged in yoga (including class attendance and personal practice). We plan to enroll 192 adults with chronic pain who are taking OAT for OUD in this 2x2x2x2 factorial experiment. CONCLUSION: Results of the study will guide development of an optimized yoga-based intervention package that maximizes dosage of yoga received. The final treatment package can be tested in a multisite efficacy trial of yoga to reduce pain interference in daily functioning in people with chronic pain who are taking OAT. TRIAL REGISTRATION: Pre-registration of the study was completed on ClinicalTrials.gov (identifier: NCT04641221).


Assuntos
Buprenorfina , Dor Crônica , Transtornos Relacionados ao Uso de Opioides , Yoga , Adulto , Humanos , Analgésicos Opioides/uso terapêutico , Buprenorfina/uso terapêutico , Dor Crônica/tratamento farmacológico , Transtornos Relacionados ao Uso de Opioides/tratamento farmacológico , Manejo da Dor
5.
mBio ; 14(5): e0141523, 2023 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-37646528

RESUMO

IMPORTANCE: Understanding how natural selection has historically shaped the traits of microbial populations comprising host microbiomes would help predict how the functions of these microbes may continue to evolve over space and time. Numerous host-associated microbes have been found to adapt to their host, sometimes becoming obligate symbionts, whereas free-living microbes are best known to adapt to their surrounding environment. Our study assessed the selective pressures of both the host environment and the surrounding external environment in shaping the functional potential of host-associated bacteria. Despite residing within the resource-rich microbiome of their hosts, we demonstrate that host-associated heterotrophic bacteria show evidence of trait selection that matches the nutrient availability of their broader surrounding environment. These findings illustrate the complex mix of selective pressures that likely shape the present-day function of bacteria found inhabiting host microbiomes. Our study lends insight into the shifts in function that may occur as environments fluctuate over time.


Assuntos
Microbiota , Bactérias/genética
6.
bioRxiv ; 2023 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-36865095

RESUMO

We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were unknown. By studying phages that encode the major phage nucleus protein chimallin, including previously sequenced yet uncharacterized phages, we discovered that chimallin-encoding phages share a set of 72 highly conserved genes encoded within seven distinct gene blocks. Of these, 21 core genes are unique to this group, and all but one of these unique genes encode proteins of unknown function. We propose that phages with this core genome comprise a novel viral family we term Chimalliviridae. Fluorescence microscopy and cryo-electron tomography studies of Erwinia phage vB_EamM_RAY confirm that many of the key steps of nucleus-based replication encoded in the core genome are conserved among diverse chimalliviruses, and reveal that non-core components can confer intriguing variations on this replication mechanism. For instance, unlike previously studied nucleus-forming phages, RAY doesn't degrade the host genome, and its PhuZ homolog appears to form a five-stranded filament with a lumen. This work expands our understanding of phage nucleus and PhuZ spindle diversity and function, providing a roadmap for identifying key mechanisms underlying nucleus-based phage replication.

7.
Cell Rep ; 42(5): 112432, 2023 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-37120812

RESUMO

We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were still to be determined. Here, we show that phages encoding the major phage nucleus protein chimallin share 72 conserved genes encoded within seven gene blocks. Of these, 21 core genes are unique to nucleus-forming phage, and all but one of these genes encode proteins of unknown function. We propose that these phages comprise a novel viral family we term Chimalliviridae. Fluorescence microscopy and cryoelectron tomography studies of Erwinia phage vB_EamM_RAY confirm that many of the key steps of nucleus-based replication are conserved among diverse chimalliviruses and reveal variations on this replication mechanism. This work expands our understanding of phage nucleus and PhuZ spindle diversity and function, providing a roadmap for identifying key mechanisms underlying nucleus-based phage replication.


Assuntos
Bacteriófagos , Erwinia , Bacteriófagos/genética , Bacteriófagos/metabolismo , Erwinia/genética , Erwinia/metabolismo , Filogenia , Genoma Viral , DNA Viral/genética , DNA Viral/metabolismo
8.
J Parasitol ; 108(5): 423-434, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-36197730

RESUMO

The acanthocephalan Neoechinorhynchus emydis has a complex life cycle and infects turtle, ostracod, and snail hosts. However, little information is available on the seasonal distribution or the effects of N. emydis on freshwater snail hosts. To address this, we examined the seasonal distribution and melanization of acanthocephalans in Planorbella cf. Planorbella trivolvis snails from a single location in north-central Oklahoma. Seasonally, prevalence of N. emydis was 0% during the winter, increased to 50% during the summer, and declined to 17% in the fall. Mean abundance exhibited more variation but generally followed a similar pattern as prevalence. More important, all acanthocephalans located within the head/foot region of snail hosts contained melaninlike pigment surrounding each worm, suggesting that snails were mounting an immunological reaction to infections with N. emydis. Snail shell diameter was greatest during the fall and decreased during the winter, indicating that larger or older snails were dying during the winter. However, because field-collected snails were commonly infected with trematodes, and snail size varied significantly with season, it was unclear whether the observed seasonal dynamics of acanthocephalan infections were a result of snail mortality resulting from snail age, parasitic infections, or a combination of factors. To control for these factors, we exposed laboratory-reared Planorbella cf. P. trivolvis snails to naturally infected ostracods in field cages for 5-wk intervals during the winter, spring, and summer. Data from snail-cage infections were consistent with the seasonal field survey such that N. emydis infections were highest in the summer (20%) and lowest (0%) in the winter, suggesting that snails were not ingesting infected ostracods during the winter. However, fewer of our laboratory-reared snails survived in field cages during winter than during spring and summer, suggesting that snails may die more often during harsh winter conditions. Finally, we conducted a laboratory survival experiment by testing the life span and egg production of field-collected snails of various sizes that were naturally infected with acanthocephalans or trematodes or both. Our snail-survival experiment indicated that snail size but not infection status with acanthocephalans or trematodes affected snail survival, with larger snails surviving a shorter amount of time than smaller snails. In addition, snails infected with trematodes laid significantly fewer eggs compared with uninfected snails or snails infected with acanthocephalans. However, we found no significant difference in the number of eggs laid by acanthocephalan-infected and uninfected snails. Although other abiotic factors still need evaluation, we suggest that the occurrence of acanthocephalans in snails throughout the year may be partially influenced by the abundance of infected ostracods that snails may be ingesting and snail population fluctuations during the year.


Assuntos
Acantocéfalos , Trematódeos , Animais , Crustáceos , Água Doce , Oklahoma/epidemiologia , Estações do Ano , Caramujos
9.
J Wildl Dis ; 57(1): 211-214, 2021 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-33635979

RESUMO

Little information exists on the occurrence of Trichinella spp. in cougars (Puma concolor) throughout North and South America. However, species distribution models (SDM) can be used to predict suitable habitat for elusive species with limited occurrence data. In 2018, we used existing occurrence data to estimate the suitable habitat and ecological drivers of Trichinella species in cougars from the southwestern region of Colorado, US from winter months. Environmental layers were constructed and an SDM was then created for the southwestern region of Colorado. The final model (area under the curve=0.73) found areas of suitable habitat for Trichinella spp. to be associated with lower elevation, lower temperature, and higher proportions of evergreen needleleaf forests and grasslands. These results might indicate potential transmission hotspots for Trichinella spp. in the southwestern region of Colorado for where cougars are consuming infected hosts and where cougars can transmit to other hosts, including humans. However, due to limited occurrence data, more suitable habitat likely exists.


Assuntos
Puma/parasitologia , Trichinella/isolamento & purificação , Triquinelose/veterinária , Animais , Colorado , Triquinelose/epidemiologia , Triquinelose/parasitologia
10.
J Parasitol ; 107(5): 739-761, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34546335

RESUMO

The role of invertebrates in some acanthocephalan life cycles is unclear because juvenile acanthocephalans are difficult to identify to species using morphology. Most reports suggest acanthocephalans from turtle definitive hosts use ostracods as intermediate hosts and snails as paratenic hosts. However, laboratory studies of the life cycle suggest that ostracods and snails are both required hosts in the life cycle. To elucidate the role of ostracods and snails in acanthocephalan life cycles better, we collected 558 freshwater snails of 2 species, including Planorbella cf. Planorbella trivolvis and Physa acuta, from 23 wetlands in Oklahoma, U.S.A., and examined them for acanthocephalan infections. Additionally, we examined 37,208 ostracods of 4 species, Physocypria sp. (morphotype 1), Cypridopsis sp., Stenocypris sp., and Physocypria sp. (morphotype 2) for juvenile acanthocephalans from 2 wetlands in Oklahoma. Juvenile acanthocephalans were morphologically characterized, and the complete internal transcribed spacer (ITS) region of nuclear rDNA was sequenced from acanthocephalans infecting 11 ostracod and 13 snail hosts. We also sampled 10 red-eared slider turtles, Trachemys scripta elegans, and 1 common map turtle, Graptemys geographica, collected from Oklahoma, Arkansas, and Texas and recovered 1,854 adult acanthocephalans of 4 species. The ITS of 17 adult acanthocephalans of 4 species from turtle hosts were sequenced and compared to juvenile acanthocephalan sequences from ostracod and snail hosts from this study and GenBank to determine conspecificity. Of the 23 locations sampled for snails, 7 (30%) were positive for juvenile acanthocephalans in the genus Neoechinorhynchus. The overall prevalence and mean intensity of acanthocephalans in Planorbella cf. P. trivolvis and P. acuta were 20% and 2 (1-6) and 2% and 1 (1), respectively. In contrast, only 1 of 4 species of ostracods, Physocypria sp. (morphotype 1), was infected with larval/juvenile Neoechinorhynchus spp. with an overall prevalence of 0.1% and a mean intensity of 1 (1-2). Although 4 species of acanthocephalans infected turtle definitive hosts, including Neoechinorhynchus chrysemydis, Neoechinorhynchus emydis, Neoechinorhynchus emyditoides, and Neoechinorhynchus pseudemydis, all the ITS sequences from cystacanths infecting snail hosts were conspecific with N. emydis. In contrast, the ITS sequences from larval/juvenile acanthocephalans from ostracods were conspecific with 2 species of acanthocephalans from turtles (N. emydis and N. pseudemydis) and 1 species of acanthocephalan from fish (Neoechinorhynchus cylindratus). These results indicate that N. emydis infects freshwater snails, whereas other species of Neoechinorhynchus appear not to infect snail hosts. We document new ostracod and snail hosts for Neoechinorhynchus species, including the first report of an ostracod host for N. pseudemydis, and we provide novel molecular barcodes that can be used to determine larva, juvenile, and adult conspecificity of Neoechinorhynchus species.


Assuntos
Acantocéfalos/genética , Crustáceos/parasitologia , Caramujos/parasitologia , Tartarugas/parasitologia , Acantocéfalos/anatomia & histologia , Acantocéfalos/isolamento & purificação , Acantocéfalos/patogenicidade , Animais , DNA de Helmintos/química , DNA de Helmintos/isolamento & purificação , Feminino , Água Doce , Masculino , Estações do Ano , Análise Espacial
11.
Vet Parasitol Reg Stud Reports ; 25: 100609, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34474802

RESUMO

Trichinella spp. infect wild carnivores throughout the world. We determined the prevalence and mean infection intensity of Trichinella spp. in bobcats (Lynx rufus) from 41 counties in Oklahoma (USA). Tongues from 306 bobcats were examined using artificial tissue digestion. The prevalence (95% confidence interval) of Trichinella spp. was 5.9% (3.7%-9.2%) in which 18 of the 301 bobcats were infected. Bobcats infected with Trichinella spp. were detected in 10 of the 41 (24.4%; 13.7%-39.5%) counties sampled. Although variable, a statistically significant difference was not detected in the prevalence of Trichinella spp. among counties where bobcats were collected. The mean (standard deviation) and median (range) infection intensity of Trichinella sp. larvae were 30.9 (39.8) and 9.6 (0.6-119.9) larvae per gram of tissue examined. Genotyping results demonstrated that 17 bobcats were infected with T. murrelliand one bobcat was infected with T. pseudospiralis. This is the first report of T. pseudospiralis in bobcats and in Oklahoma. These data suggest the bobcat, as an obligate carnivore, is likely an important host in maintaining T. murrelli sylvatic cycles in Oklahoma.


Assuntos
Carnívoros , Lynx , Trichinella , Triquinelose , Animais , Oklahoma/epidemiologia , Trichinella/genética , Triquinelose/epidemiologia , Triquinelose/veterinária
12.
Exp Gerontol ; 96: 155-161, 2017 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-28668481

RESUMO

Reduced reproduction and dietary restriction each extend lifespan in many animal models, but possible contributions of nutrient oxidation and allocation are largely unknown. Ovariectomy and eating 70% of ad libitum-feeding each extend lifespan in lubber grasshoppers. When feeding levels between the two groups are matched, ovariectomy increases fat and protein storage while dietary restriction reduces fat storage. Because of these disparities in nutrient investment, metabolism may differ between these two life-extending treatments. Therefore, we examined the allocation and organismal oxidation of one representative of each macronutrient class: leucine, oleic acid, and glucose. Ovariectomy and dietary restriction each increased oxidation of dietary leucine. Dietary leucine may play a special role in aging because amino acids stimulate cellular growth. Speeding oxidation of leucine may attenuate cellular growth. Allocation of leucine to muscle was the clearest difference between ovariectomy and dietary restriction in this study. Ovariectomy reduced allocation of leucine to femur muscle, whereas dietary restriction increased allocation of leucine to femur muscle. This allocation likely corresponds to muscle maintenance for locomotion, suggesting dietary restriction increases support for locomotion, perhaps to search for food. Last, ovariectomy decreased oxidation of dietary oleic acid and glucose, perhaps to save them for storage, but the site of storage is unclear. This study suggests that the oxidation of branched-chain amino acids may be an underappreciated mechanism underlying lifespan extension.


Assuntos
Restrição Calórica , Leucina/metabolismo , Longevidade/fisiologia , Análise de Variância , Animais , Glicemia/metabolismo , Gafanhotos , Músculo Esquelético/fisiologia , Ácido Oleico/metabolismo , Ovariectomia , Oxirredução , Respiração , Serina-Treonina Quinases TOR/metabolismo
13.
J Thorac Cardiovasc Surg ; 151(6): 1718-1728.e5, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26979916

RESUMO

OBJECTIVE: Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) commonly dilate asymmetrically compared with patients with tricuspid aortic valve (TAV). This discrepancy in aneurysm geometry led us to hypothesize that microarchitectural differences underlie the observed asymmetric dilatation pattern. The purpose of this study was to characterize the microarchitectural distinctions of the extracellular matrix of the 2 phenotypes with a focus on the proportion of radially oriented elastin and collagen fibers in different circumferential aortic regions. METHODS: Aortic tissue rings were obtained just distal to the sinotubular junction from patients with BAV or TAV undergoing elective aneurysm repair. They were sectioned into three circumferentially based regions according to adjacent aortic sinus segment (left coronary sinus [L], right coronary sinus [R], or noncoronary sinus [N]). Multiphoton microscopy was used to quantify and characterize the number of radially oriented elastin and collagen fibers. RESULTS: There were fewer radially oriented fibers in medial region N and medial-intimal region R of BAV-ATAAs when compared with TAV-ATAAs (medial region N, amplitude of angular undulation of elastin = 10.67° ± 1.35° vs 15.58° ± 1.91°; P = .041; medial-intimal region R, amplitude of angular undulation of elastin = 9.83° ± 0.83° vs 14.72° ± 1.64°; P = .015). Conversely, fibers became more radially oriented in the medial-intimal region L of BAV-ATAA when compared with TAV-ATAA (amplitude of angular undulation of collagen = 18.67° ± 0.95° vs 14.56° ± 1.37°; P = .041). CONCLUSIONS: The differential pattern of fiber orientation noted between L and N-R regions help explain the unique pattern of greater curvature dilatation of BAV-ATAA. The distinctions noted in matrix microarchitecture may form the basis of differing aneurysm geometries and aortic wall integrities in ATAAs arising in these different valve morphologies.


Assuntos
Aorta Torácica/patologia , Aneurisma da Aorta Torácica/etiologia , Valva Aórtica/anormalidades , Matriz Extracelular/ultraestrutura , Doenças das Valvas Cardíacas/complicações , Adulto , Idoso , Idoso de 80 Anos ou mais , Aorta Torácica/fisiopatologia , Aneurisma da Aorta Torácica/diagnóstico , Aneurisma da Aorta Torácica/fisiopatologia , Doença da Válvula Aórtica Bicúspide , Elasticidade , Feminino , Doenças das Valvas Cardíacas/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade
14.
J Biomech ; 47(5): 935-943, 2014 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-24524988

RESUMO

The aorta possesses a micro-architecture that imparts and supports a high degree of compliance and mechanical strength. Alteration of the quantity and/or arrangement of the main load-bearing components of this micro-architecture--the elastin and collagen fibers--leads to mechanical, and hence functional, changes associated with aortic disease and aging. Therefore, in the future, the ability to rigorously characterize the wall fiber micro-architecture could provide insight into the complicated mechanisms of aortic wall remodeling in aging and disease. Elastin and collagen fibers can be observed using state-of-the-art multi-photon microscopy. Image-analysis algorithms have been effective at characterizing fibrous constructs using various microscopy modalities. The objective of this study was to develop a custom MATLAB-language automated image-based analysis tool to describe multiple parameters of elastin and collagen micro-architecture in human soft fibrous tissue samples using multi-photon microscopy images. Human aortic tissue samples were used to develop the code. The tool smooths, cleans and equalizes fiber intensities in the image before segmenting the fibers into a binary image. The binary image is cleaned and thinned to a fiber skeleton representation of the image. The developed software analyzes the fiber skeleton to obtain intersections, fiber orientation, concentration, porosity, diameter distribution, segment length and tortuosity. In the future, the developed custom image-based analysis tool can be used to describe the micro-architecture of aortic wall samples in a variety of conditions. While this work targeted the aorta, the software has the potential to describe the architecture of other fibrous materials, tube-like networks and connective tissues.


Assuntos
Aorta/anatomia & histologia , Aorta/química , Elastina/análise , Colágenos Fibrilares/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Algoritmos , Tecido Conjuntivo/anatomia & histologia , Tecido Conjuntivo/química , Matriz Extracelular/química , Feminino , Humanos , Masculino , Microscopia , Pessoa de Meia-Idade , Software , Suporte de Carga
15.
J Biomech ; 46(16): 2787-94, 2013 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-24075403

RESUMO

It was recently demonstrated by our group that the delamination strength of ascending thoracic aortic aneurysms (ATAA) was lower than that of control (CTRL, non-aneurysmal) ascending thoracic aorta (ATA), and the reduced strength was more pronounced among bicuspid (BAV) vs. tricuspid aortic valve (TAV) patients, suggesting a different risk of aortic dissection for BAV patients. We hypothesized that aortic valve morphologic phenotype predicts fiber micro-architectural anomalies in ATA. To test the hypothesis, we characterized the micro-architecture in the longitudinal-radial (Z-RAD) and circumferential-radial (Θ-RAD) planes of human ATA tissue that was artificially dissected medially. The outer and inner-media of CTRL-ATA, BAV-ATAA and TAV-ATAA were imaged using multi-photon microscopy in the Z-RAD and Θ-RAD planes to observe collagen and elastin. Micrographs were processed using an image-based tool to quantify several micro-architectural characteristics. In the outer-media of BAV-ATAA, elastin was more undulated and less aligned about the Θ-axis when compared with CTRL-ATA, which is consistent with increased tensile stretch at inflection point of Θ-strips of adventitial-medial half of BAV-ATAA (1.28) when compared with CTRL-ATA (1.13). With increasing age, collagen became more undulated about the Z-axis within the outer-media of TAV-ATAA, and elastin became more oriented in the Z-axis and collagen less radially-oriented within the inner-media of TAV-ATAA. This discrepancy in the micro-architecture with fibers in the inner layers being more stretched and with disrupted radially-oriented components than fibers in the outer layers may be associated with the development, progression and vascular remodeling in aneurysms arising in TAV patients.


Assuntos
Aneurisma da Aorta Torácica/patologia , Dissecção Aórtica/patologia , Valva Aórtica/patologia , Colágeno/análise , Elastina/análise , Túnica Íntima/patologia , Túnica Média/patologia , Análise de Variância , Dissecção Aórtica/fisiopatologia , Aneurisma da Aorta Torácica/fisiopatologia , Ruptura Aórtica/patologia , Ruptura Aórtica/fisiopatologia , Valva Aórtica/fisiopatologia , Fenômenos Biomecânicos , Feminino , Humanos , Masculino , Microscopia de Fluorescência por Excitação Multifotônica , Pessoa de Meia-Idade , Fenol , Túnica Íntima/química , Túnica Média/química
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