Spherical wave illumination scanning digital holographic profilometry.

In this work, we proposed what we believe to be a novel scanning solution for the assessment of high-NA samples, referred to as spherical-wave illumination scanning digital holographic profilometry (SWS-DHP). This approach introduces a 2F optimization methodology, based on the measurement of the focal length of the object to determine the spherical component of the scanning. Furthermore, re-optimization of 2F, whether it needs to be operated depends on the measured object's NA to inspect more information. Meanwhile, utilizing phase space analysis shows SWS superiority in information transfer for high-NA samples compared to plane-wave illumination scanning. In addition, this method introduces a shape reconstruction algorithm with volumetric aberration compensation based on the propagation of the aberrated object and illumination waves to obtain high-quality measurements. Finally, the imaging merits of SWS-DHP were proved through simulations and were experimentally verified for the object of NA up to 0.87.

Digital holographic content manipulation for wide-angle holographic near-eye displays.

In recent years, the development of holographic near-eye displays (HNED) has surpassed the progress of digital hologram recording systems, especially in terms of wide-angle viewing capabilities. Thus, there is capture-display parameters incompatibility, which makes it impossible to reconstruct recorded objects in wide-angle display. This paper presents a complete imaging chain extending the available content for wide-angle HNED of pupil and non-pupil configuration with narrow-angle digital holograms of real objects. To this end, a new framework based on the phase-space approach is proposed that includes a set of affine transformations required to account for all differences in capture-display cases. The developed method allows free manipulation of the geometry of reconstructed objects, including axial and lateral positioning and size scaling. At the same time, it has a low computational effort. The presented work is supported with non-paraxial formulas developed using the phase-space approach, enabling accurate tracing of the holographic signal, its reconstruction, and measuring appearing deformations. The applicability of the proposed hologram manipulation method is proven with experimental results of digital hologram reconstruction in wide-angle HNED.

High diffraction efficiency complex amplitude modulation technique by using antiwave functions.

Complex amplitude modulation (CAM) is a single-step technique that codes the amplitude and phase of a diffracted optical field into a real function. Loading this function onto a phase-only spatial light modulator enables the reconstruction of 3D images. However, the obtained images have poor brightness because of the low diffraction efficiency. In this work, we show a novel solution, to the best of our knowledge, that overcomes this limitation. This is done by employing antiwave complex exponential functions, which modify the spectrum of the diffracted field. Proper configuration of these functions allows transferring energy across the diffraction spectrum to the informative diffraction order, which significantly improves the brightness of the reconstructed image. Simulations and experiments prove the efficiency of the proposed method.

Eyebox expansion with accurate hologram generation for wide-angle holographic near-eye display.

Small eyebox in wide-angle holographic near-eye display is a severe limitation for 3D visual immersion of the device. In this paper, an opto-numerical solution for extending the eyebox size in these types of devices is presented. The hardware part of our solution expands the eyebox by inserting a grating of frequency fg within a non-pupil forming display configuration. The grating multiplies eyebox, increasing the possible eye motion. The numerical part of our solution is an algorithm that enables proper coding of wide-angle holographic information for projecting correct object reconstruction at arbitrary eye position within the extended eyebox. The algorithm is developed through the employment of the phase-space representation, which facilitates the analysis of the holographic information and the impact of the diffraction grating in the wide-angle display system. It is shown that accurate encoding of the wavefront information components for the eyebox replicas is possible. In this way, the problem of missing or incorrect views in wide angle near-eye display with multiplied eyeboxes is efficiently solved. Moreover, this study investigates the space-frequency relation between the object and the eyebox and how the hologram information is shared between eyebox replicas. The functionality of our solution is tested experimentally in an augmented reality holographic near-eye display that has maximum field of view of 25.89°. Obtained optical reconstructions demonstrate that correct object view is obtained for arbitrary eye position within extended eyebox.

JPEG Pleno holography presents the numerical reconstruction software for holograms: an excursion in holographic views.

Digital reconstructions of numerical holograms enable data visualization and serve a multitude of purposes ranging from microscopy to holographic displays. Over the years, many pipelines have been developed for specific hologram types. Within the standardization effort of JPEG Pleno holography, an open-source MATLAB toolbox was developed that reflects the best current consensus. It can process Fresnel, angular spectrum, and Fourier-Fresnel holograms with one or more color channels; it also allows for diffraction-limited numerical reconstructions. The latter provides a way to reconstruct holograms at their intrinsic physical instead of an arbitrarily chosen numerical resolution. The Numerical Reconstruction Software for Holograms v10 supports all large public data sets featured by UBI, BCOM, ETRI, and ETRO, in their native and vertical off-axis binary forms. Through the release of this software, we hope to improve the reproducibility of research, thus enabling consistent comparison of data between research groups and the quality of specific numerical reconstructions.

Structured illumination phase and fluorescence microscopy for bioimaging.

This study presents a dual-modality microscopic imaging approach that combines quantitative phase microscopy and fluorescence microscopy based on structured illumination (SI) to provide structural and functional information for the same sample. As the first imaging modality, structured illumination digital holographic microscopy (SI-DHM) is implemented along the transmission beam path. SI-DHM acts as a label-free, noninvasive approach and provides high-contrast and quantitative phase images utilizing the refractive index contrast of the inner structures of samples against the background. As the second imaging modality, structured illumination (fluorescence) microscopy (SIM) is constructed along the reflection beam path. SIM utilizes fluorescent labeling and provides super-resolution images for specific functional structures of samples. We first experimentally demonstrated phase imaging of SI-DHM on rice leaves and fluorescence (SIM) imaging on mouse kidney sections. Then, we demonstrated dual-modality imaging of biological samples, using DHM to acquire the overall cell morphology and SIM to obtain specific functional structures. These results prove that the proposed technique is of great importance in biomedical studies, such as providing insight into cell physiology by visualizing and quantifying subcellular structures.

Off-axis propagation algorithm for partial reconstruction of wide-angle computer-generated holograms.

A method for reconstruction of partial off-axis areas of arbitrary size for wide-angle viewing computer generated holograms is presented. Proposed method employs paraxial spherical phase factors and modified propagation kernels. This significantly reduces the numerical space-bandwidth product needed for off-axis wave field calculations, which makes it an efficient propagation method. As a result, propagated wavefields of high-off axis and large size output windows can be obtained. To that end, a phase-space analysis for obtaining the proper condition for implementing spatial-frequency zero-padding for accurate wavefield propagation is carried out. Hence, suppression of aliased components and high spatial resolution is possible. Nevertheless, proposed algorithm faces a computer memory bottleneck when reconstructing very large off-axis areas due to too extensive zero-padding needed. To solve this problem, a memory optimized tiling implementation is introduced. Utility of the developed propagation tools are proven by partial reconstructions from a high-resolution hologram. The size of the reconstructions areas ranges from 100 × 100 mm2 up to 550 × 550 mm2.

LED near-eye holographic display with a large non-paraxial hologram generation.

In this paper, two solutions are proposed to improve the quality of a large image that is reconstructed in front of the observer in a near-eye holographic display. One of the proposed techniques, to the best of our knowledge, is the first wide-angle solution that successfully uses a non-coherent LED source. It is shown that the resulting image when employing these types of sources has less speckle noise but a resolution comparable to that obtained with coherent light. These results are explained by the developed theory, which also shows that the coherence effect is angle varying. Furthermore, for the used pupil forming display architecture, it is necessary to compute a large virtual nonparaxial hologram. We demonstrate that for this hologram there exists a small support region that has a frequency range capable of encoding information generated by a single point of the object. This small support region is beneficial since it enables to propose a wide-angle rigorous CGH computational method, which allows processing very dense cloud of points that represents three-dimensional objects. This is our second proposed key development. To determine the corresponding support region, the concept of local wavefront spatial curvature is introduced, which is proportional to the tangent line to the local spatial frequency of the spherical wavefront. The proposed analytical solution shows that the size of this area strongly depends on the transverse and longitudinal coordinate of the corresponding object point.

Large-field structured illumination microscopy based on 2D grating and a spatial light modulator.

Structured illumination microscopy (SIM) has been widely used in biological research due to its merits of fast imaging speed, minimal invasiveness, super-resolution, and optical sectioning imaging capability. However, the conventional SIM that uses a spatial light modulator (SLM) for fringe projection often has a limited imaging field of view. Herein, we report a large-field SIM technique that combines a 2D grating for fringe pattern projection and an SLM for selecting fringe orientation and performing phase shifting digitally. The proposed SIM technique breaks the bottleneck of fringe number limited by the digital projection devices, while maintaining the advantage of high-speed (digital) phase shifting of conventional SIM. The method avoids the pixilation and dispersion effects of the SLMs. Finally, a 1.8-fold resolution enhancement in a large field of 690 × 517 µm2 under a 20×/NA0.75 objective is experimentally demonstrated. The proposed technique can be widely applied to biology, chemistry, and industry.

Single-shot digital multiplexed holography for the measurement of deep shapes.

This work develops a single-shot holographic profilometer that enables shape characterization of discontinuous deep surfaces. This is achieved by combining hologram frequency multiplexing and an illumination technique of complex amplitude in multi-incidence angle profilometer. Object illumination is carried out from seven directions simultaneously, where the radial angular coordinates of illumination plane waves obey the geometric series. It is shown that: (i) the illumination pattern provides the required frequency separation of all object wavefronts in transverse frequency space, which is necessary for hologram demultiplexing, and (ii) numerical generation of longitudinal scanning function (LSF) is possible, which has large measurement range, high axial resolution, and small side lobes. Low side lobes of LSF and the developed multiplexed field dependent aberration compensation method are essential to minimize the negative influence of speckle noise of single-shot capture on the measurement result. The utility of the proposed method is demonstrated with experimental measurement of heights of two step-like objects.

Fourier horizontal parallax only computer and digital holography of large size.

Registration and reconstruction of high-quality digital holograms with a large view angle are intensive computer tasks since they require the space-bandwidth product (SBP) of the order of tens of gigapixels or more. This massive use of SBP severely affects the storing and manipulation of digital holograms. In order to reduce the computer burden, this work focuses on the generation and reconstruction of very large horizontal parallax only digital holograms (HPO-DHs). It is shown that these types of holograms can preserve high quality and large view angle in x direction while keeping a low use of SBP. This work first proposes a numerical technique that allows calculating very large HPO-DHs with large pixel size by merging the Fourier holography and phase added stereogram algorithm. The generated Fourier HPO-DHs enable accurate storing of holographic data from 3D objects. To decode the information contained in these Fourier HPO-DHs (FHPO-DHs), a novel angular spectrum (AS) technique that provides an efficient use of the SBP for reconstruction is proposed. Our reconstruction technique, which is called compact space bandwidth AS (CSW-AS), makes use of cylindrical parabolic waves that solve sampling issues of FHPO-DHs and AS. Moreover, the CSW-AS allows for implementing zero-padding for accurate wavefield reconstructions. Hence, suppression of aliased components and high spatial resolution is possible. Notably, the imaging chain of Fourier HPO-DH enables efficient calculation, reconstruction and storing of HPO holograms of large size. Finally, the accuracy and utility of the developed technique is proved by both numerical and optical reconstructions.

Photorealistic computer generated holography with global illumination and path tracing.

Computer generated holography (CGH) algorithms come in many forms, with different trade-offs in terms of visual quality and calculation speed. However, no CGH algorithm to date can accurately account for all 3D visual cues simultaneously, such as occlusion, shadows, continuous parallax, and precise focal cues, without view discretization. The aim is to create photorealistic CGH content, not only for display purposes but also to create reference data for comparing and testing CGH and compression algorithms. We propose a novel algorithm combining the precision of point-based CGH with the accurate shading and flexibility of ray-tracing algorithms. We demonstrate this by creating a scene with global illumination, soft shadows, and precise occlusion cues, implemented with OptiX and CUDA.

Wide angle holographic video projection display.

Holographic projection displays provide high diffraction efficiency. However, they have a limited projection angle. This work proposes a holographic projection display with a wide angle, which gives an image of size 306mm×161mm at 700 mm and reduced speckle noise. The solution uses single Fourier lens imaging with a frequency filter and hologram generation utilizing complex coding and nonparaxial diffraction. The experiment was performed with a 4K phase-only spatial light modulator (SLM) to prove the high efficiency of the developed numerical tools. Optical reconstruction shows high resolution and high image quality achieved from a single frame. Hence, displaying video at a full frame rate of the SLM is possible.

Fast and accurate phase-unwrapping algorithm based on the transport of intensity equation: reply.

In [Appl. Opt.56, 7079 (2017)APOPAI0003-693510.1364/AO.56.007079], a phase-unwrapping algorithm has been reported that is based on the transport of intensity (TIE) equation. Section 3 of that manuscript presented one way to derive an analytical expression for the axial intensity derivative using the paraxial angular spectrum (AS) method. In the recent comment by Yoneda et al. [Appl. Opt.60, 7500 (2021)APOPAI0003-693510.1364/AO.417146], the authors misunderstand the utility of the angular spectrum method and conclude that the corresponding derivation is ambiguous. In this response, we take the opportunity to correct a typo that clarifies the calculation of the axial derivative of the intensity.

Multi-Incidence Holographic Profilometry for Large Gradient Surfaces with Sub-Micron Focusing Accuracy.

Surface reconstruction for micro-samples with large discontinuities using digital holography is a challenge. To overcome this problem, multi-incidence digital holographic profilometry (MIDHP) has been proposed. MIDHP relies on the numerical generation of the longitudinal scanning function (LSF) for reconstructing the topography of the sample with large depth and high axial resolution. Nevertheless, the method is unable to reconstruct surfaces with large gradients due to the need of: (i) high precision focusing that manual adjustment cannot fulfill and (ii) preserving the functionality of the LSF that requires capturing and processing many digital holograms. In this work, we propose a novel MIDHP method to solve these limitations. First, an autofocusing algorithm based on the comparison of shapes obtained by the LSF and the thin tilted element approximation is proposed. It is proven that this autofocusing algorithm is capable to deliver in-focus plane localization with submicron resolution. Second, we propose that wavefield summation for the generation of the LSF is carried out in Fourier space. It is shown that this scheme enables a significant reduction of arithmetic operations and can minimize the number of Fourier transforms needed. Hence, a fast generation of the LSF is possible without compromising its accuracy. The functionality of MIDHP for measuring surfaces with large gradients is supported by numerical and experimental results.

Multi-incidence digital holographic profilometry with high axial resolution and enlarged measurement range.

In this work, multi-incident digital holographic profilometry for microscale measurements is presented. This technique assembles the set of object fields from captured holograms for generation of the longitudinal scanning function (LSF). Numerical propagation is used for refocusing, and thus, the LSF can be determined at any given plane along the optical axis. The LSF takes maximum value for in focus object points, which are used to obtain full-field height distribution of the sample. This principle is the base of proposed measurement technique. Three capturing holograms strategies, which give control over the shape of the LSF, unambiguous measurement range, axial resolution, and noise immunity, are discussed. The conclusions of this work are supported by numerical and experimental results.

Suitability analysis of holographic vs light field and 2D displays for subjective quality assessment of Fourier holograms.

Measuring the impact of compression on the reconstruction quality of holograms remains a challenge. A public subjectively-annotated holographic data set that allows for testing the performance of compression techniques and quality metrics is presented, in addition to a subjective visual quality assessment methodology. Moreover, the performance of the quality assessment procedures is compared for holographic, regular 2D and light field displays. For these experiments, a double-stimulus, multi-perspective, multi-depth testing methodology was designed and implemented. Analysis of the quality scores indicated that in the absence of a suitable holographic display and under the presented test conditions, non-holographic displays can be deployed to display numerically reconstructed holograms for visual quality assessment tasks.

Fast multiple-scattering holographic tomography based on the wave propagation method.

We develop a time-efficient computation scheme for a holographic tomography reconstruction technique that accounts for multiple scattering by applying the forward model based on the wave propagation method (WPM). The computational efficiency is achieved by employing adjoint equations for calculation of the gradient of the data fidelity term in the gradient descent optimization procedure. In the paper we provide a general computation scheme that is suitable for various forward models that can be represented in the form of an iterative equation. Next, we provide the complete solution for the time-efficient reconstruction utilizing WPM. In the considered reconstruction case, the proposed algorithm enables the 114-fold speed-up of computations with respect to the original tomographic method.

Accurate reconstruction of horizontal parallax-only holograms by angular spectrum and efficient zero-padding.

Accurate reconstruction of digital holograms that are large in the x direction and small in the y direction, known as horizontal parallax only digital hologram (HPO-DH), must be carried out by non-paraxial propagation approaches such as the classical angular spectrum (AS) method. However, the required space-bandwidth product (SBP) for reconstruction of HPO-DHs requires billions of pixels, which is computationally intensive. Moreover, application of zero-padding for removing aliasing components would generate an unbearable computational burden. In this work, a novel AS technique that reconstructs non-paraxial HPO-DHs with low SBP is proposed. The proposed technique first employs the multi-Fourier transform plane propagation method, which avoids the increase of size in the vertical direction of the HPO-DH to be processed. The second ingredient for field calculation is coherent superposition of vertical tiles formed from the multi-Fourier transform calculations. The described methodology enables reconstruction of HPO-DHs with the AS method and reduced SBP. Efficient managing of the SBP allows implementing zero-padding strategies in the x direction. It is shown that the padding strategies can be implemented in the frequency, space, and space-frequency domains. Hence, suppression of aliased components and increase of the spatial resolution is possible at the same time. Finally, the accuracy and utility of the developed technique is proved by both numerical simulations and experiments.

Color LED DMD holographic display with high resolution across large depth.

Holographic displays employing digital micromirror devices (DMDs) reconstruct 3D images at high diffraction orders. For LED displays, this geometry introduces large dispersion at the DMD surface, reducing image resolution and depth. This work proposes a color DMD LED holographic display with dispersion compensation utilizing an additional diffraction grating in an illumination module. The solution allows to obtain image depth up to 100 mm, which is comparable to the one achieved by a liquid crystal spatial light modulator, where the limiting factor is spatial coherence of the source. Experimental comparison of the results obtained with the laser and LED source gives evidence of effective speckle noise reduction, even from a single frame.