Involvement of GLCCI1 in mouse spermatogenesis.

Spermatid production is a complex regulatory process in which coordination between hormonal control and apoptosis plays a pivotal role in maintaining a balanced number of sperm cells. Apoptosis in spermatogenesis is controlled by pro-apoptotic and anti-apoptotic molecules. Hormones involved in the apoptotic process during spermatogenesis include gonadotrophins, sex hormones, and glucocorticoid (GC). GC acts broadly as an apoptosis inducer by binding to its receptor (glucocorticoid receptor: GR) during organ development processes, such as spermatogenesis. However, the downstream pathway induced in GC-GR signaling and the apoptotic process during spermatogenesis remains poorly understood. We reported previously that GC induces full-length glucocorticoid-induced transcript 1 (GLCCI1-long), which functions as an anti-apoptotic mediator in thymic T cell development. Here, we demonstrate that mature murine testis expresses a novel isoform of GLCCI1 protein (GLCCI1-short) in addition to GLCCI1-long. We demonstrate that GLCCI1-long is expressed in spermatocytes along with GR. In contrast, GLCCI1-short is primarily expressed in spermatids where GR is absent; instead, the estrogen receptor is expressed. GLCCI1-short also binds to LC8, which is a known mediator of the anti-apoptotic effect of GLCCI1-long. A luciferase reporter assay revealed that ß-estradiol treatment synergistically increased Glcci1-short promotor-driven luciferase activity in Erα-overexpressing cells. Together with the evidence that the conversion of testosterone to estrogen is preceded by aromatase expression in spermatids, we hypothesize that estrogen induces GLCCI1-short, which, in turn, may function as a novel anti-apoptotic mediator in mature murine testis.

CH4 Synthesis from CO2 and H2O of an Electron Source over Rh-Ru Cocatalysts Loaded on NaTaO3:Sr Photocatalysts.

CO2 reduction as an artificial photosynthetic system is a promising technology to produce green energies and chemicals because it uses light energy to convert H2O and CO2 into valuable products such as CO, HCOOH, CH3OH, CH4, and preferably higher hydrocarbons. In photocatalytic reduction, water should be used as hydrogen and electron sources for CO2 reduction. Moreover, CH4 formation is an attractive and challenging topic because of the eight-electron-reducing product of CO2. Herein, we report the development of a new Rh-Ru cocatalyst decorated on an alkaline earth-doped NaTaO3 surface for the photocatalytic CO2 reduction to form CH4 using water as an electron donor. CH4 was obtained by a photocatalytic "uphill" reaction of CO2 reduction using Rh-Ru cocatalyst-loaded NaTaO3:Sr, water, and CO2 in an aqueous suspension system. About 10% of a selectivity (electronic efficiency) was obtained for CH4 formation under ambient conditions accompanied with O2 evolution of the oxidation product of H2O.

Carbon Nitride Loaded with an Ultrafine, Monodisperse, Metallic Platinum-Cluster Cocatalyst for the Photocatalytic Hydrogen-Evolution Reaction.

For the realization of a next-generation energy society, further improvement in the activity of water-splitting photocatalysts is essential. Platinum (Pt) is predicted to be the most effective cocatalyst for hydrogen evolution from water. However, when the number of active sites is increased by decreasing the particle size, the Pt cocatalyst is easily oxidized and thereby loses its activity. In this study, a method to load ultrafine, monodisperse, metallic Pt nanoclusters (NCs) on graphitic carbon nitride is developed, which is a promising visible-light-driven photocatalyst. In this photocatalyst, a part of the surface of the Pt NCs is protected by sulfur atoms, preventing oxidation. Consequently, the hydrogen-evolution activity per loading weight of Pt cocatalyst is significantly improved, 53 times, compared with that of a Pt-cocatalyst loaded photocatalyst by the conventional method. The developed method is also effective to enhance the overall water-splitting activity of other advanced photocatalysts such as SrTiO3 and BaLa4 Ti4 O15 .

CO2 Reduction Using Water as an Electron Donor over Heterogeneous Photocatalysts Aiming at Artificial Photosynthesis.

Photocatalytic and photoelectrochemical CO2 reduction of artificial photosynthesis is a promising chemical process to solve resource, energy, and environmental problems. An advantage of artificial photosynthesis is that solar energy is converted to chemical products using abundant water as electron and proton sources. It can be operated under ambient temperature and pressure. Especially, photocatalytic CO2 reduction employing a powdered material would be a low-cost and scalable system for practical use because of simplicity of the total system and simple mass-production of a photocatalyst material.In this Account, single particulate photocatalysts, Z-scheme photocatalysts, and photoelectrodes are introduced for artificial photosynthetic CO2 reduction. It is indispensable to use water as an electron donor (i.e., reasonable O2 evolution) but not to use a sacrificial reagent of a strong electron donor, for achievement of the artificial photosynthetic CO2 reduction accompanied by ΔG > 0. Confirmations of O2 evolution, a ratio of reacted e- to h+ estimated from obtained products, a turnover number, and a carbon source of a CO2 reduction product are discussed as the key points for evaluation of photocatalytic and photoelectrochemical CO2 reduction.Various metal oxide photocatalysts with wide band gaps have been developed for water splitting under UV light irradiation. However, these bare metal oxide photocatalysts without a cocatalyst do not show high photocatalytic CO2 reduction activity in an aqueous solution. The issue comes from lack of a reaction site for CO2 reduction and competitive reaction between water and CO2 reduction. This raises a key issue to find a cocatalyst and optimize reaction conditions defining this research field. Loading a Ag cocatalyst as a CO2 reduction site and NaHCO3 addition for a smooth supply of hydrated CO2 molecules as reactant are beneficial for efficient photocatalytic CO2 reduction. Ag/BaLa4Ti4O15 and Ag/NaTaO3:Ba reduce CO2 to CO as a main reduction reaction using water as an electron donor even in just water and an aqueous NaHCO3 solution. A Rh-Ru cocatalyst on NaTaO3:Sr gives CH4 with 10% selectivity (Faradaic efficiency) based on the number of reacted electrons in the photocatalytic CO2 reduction accompanied by O2 evolution by water oxidation.Visible-light-responsive photocatalyst systems are indispensable for efficient sunlight utilization. Z-scheme systems using CuGaS2, (CuGa)1-xZn2xS2, CuGa1-xInxS2, and SrTiO3:Rh as CO2-reducing photocatalyst, BiVO4 as O2-evolving photocatalyst, and reduced graphene oxide (RGO) and Co-complex as electron mediator or without an electron mediator are active for CO2 reduction using water as an electron donor under visible light irradiation. These metal sulfide photocatalysts have the potential to take part in Z-scheme systems for artificial photosynthetic CO2 reduction, even though their ability to extract electrons from water is insufficient.A photoelectrochemical system using a photocathode is also attractive for CO2 reduction under visible light irradiation. For example, p-type CuGaS2, (CuGa)1-xZn2xS2, Cu1-xAgxGaS2, and SrTiO3:Rh function as photocathodes for CO2 reduction under visible light irradiation. Moreover, introducing a conducting polymer as a hole transporter and surface modification with Ag and ZnS improve photoelectrochemical performance.

Examination of photocatalytic Z-scheme system for overall water splitting with its electronic structure.

Although the solar-to-hydrogen (STH) conversion efficiency of a photocatalytic Z-scheme system for overall water-splitting with a solid-state electron mediator composed of a hydrogen evolution cocatalyst (HEC) nanoparticles/hydrogen evolution photocatalyst (HEP) particle layer with an Rh,La-codoped SrTiO3/conductor with an Au/oxygen evolution photocatalyst (OEP) particle layer with Mo-doped BiVO4/oxygen evolution cocatalyst (OEC) nanoparticles reached the highest value (1.1%) in 2016, it was still insufficient for practical application, resulting in a proposal in a previous paper to develop HEP and OEP particles with longer wavelength absorption edges. While progress has been rather slow since then, the Z-scheme system has been analyzed in this paper from a new point of view, i.e., the electronic structure of the system on the basis of solid-state physics, in order to seek for new ideas to enhance its STH conversion efficiency. In addition to the proposal in the previous paper, new ideas in this paper include the formation of a built-in potential to enhance electron (positive hole) transfer from the HEP (OEP) to the HEC (OEC) by putting positive (negative) charges on the HEC (OEC) nanoparticles, enhancement of the reduction (oxidation) of water by an electron (a positive hole) transferred from the HEP (OEP) to the HEC (OEC) by using the quantum-size effect of HEC and OEC nanoparticles, enhancement of the transfer of a photo-created positive hole (electron) from the HEP (OEP) to the conductor by controlling the Schottky barrier between them, and enhancement of the movement of electronic charge carriers together with depression of their recombination in highly doped HEP and OEP particles by the use of ionic relaxation processes in the particles.

Understanding the reaction mechanism and kinetics of photocatalytic oxygen evolution on CoOx-loaded bismuth vanadate.

Photocatalytic water splitting for green hydrogen production is hindered by the sluggish kinetics of oxygen evolution reaction (OER). Loading a co-catalyst is essential for accelerating the kinetics, but the detailed reaction mechanism and role of the co-catalyst are still obscure. Here, we focus on cobalt oxide (CoOx) loaded on bismuth vanadate (BiVO4) to investigate the impact of CoOx on the OER mechanism. We employ photoelectrochemical impedance spectroscopy and simultaneous measurements of photoinduced absorption and photocurrent. The reduction of V5+ in BiVO4 promotes the formation of a surface state on CoOx that plays a crucial role in the OER. The third-order reaction rate with respect to photohole charge density indicates that reaction intermediate species accumulate in the surface state through a three-electron oxidation process prior to the rate-determining step. Increasing the excitation light intensity onto the CoOx-loaded anode improves the photoconversion efficiency significantly, suggesting that the OER reaction at dual sites in an amorphous CoOx(OH)y layer dominates over single sites. Therefore, CoOx is directly involved in the OER by providing effective reaction sites, stabilizing reaction intermediates, and improving the charge transfer rate. These insights help advance our understanding of co-catalyst-assisted OER to achieve efficient water splitting.

A possible function of Nik-related kinase in the labyrinth layer of delayed delivery mouse placentas.

In mice and humans, Nik-related protein kinase (Nrk) is an X-linked gene that encodes a serine/threonine kinase belonging to GCK group 4. Nrk knockout (Nrk KO) mice exhibit delayed delivery, possibly due to defective communication between the Nrk KO conceptus and its mother. However, the mechanism of delayed labor remains largely unknown. Here, we found that in pregnant mothers with the Nrk KO conceptus, the serum progesterone (P4) and placental lactogen (PL-2) concentrations in late pregnancy were higher than those in the wild type. Moreover, we demonstrated that Nrk is expressed in trophoblast giant cells (TGCs) and syncytiotrophoblast-2 (SynT-2) in the labyrinth layer of the mouse placenta. In the human placenta, NRK is also expressed in Syn-T in villi. Both human Syn-T and mouse TGCs of the labyrinth layer are present within fetal tissues that are in direct contact with the maternal blood. The labyrinth layer of the Nrk KO conceptus was gigantic, with enlarged cytoplasm and Golgi bodies in the TGCs. To investigate the function of Nrk in the labyrinth layer, a differentially expressed gene (DEG) analysis was performed. The DEG analysis revealed that labor-promoting factors, such as prostaglandins, were decreased, and pregnancy-maintaining factors, such as the prolactin family and P4 receptor, were increased. These findings suggest that the Nrk KO mice exhibit delayed delivery owing to high P4 concentrations caused by the hypersecretion of pregnancy-maintaining factors, such as PL-2, from the placenta.

A Novel Mouse Model of Idiopathic Nephrotic Syndrome Induced by Immunization with the Podocyte Protein Crb2.

BACKGROUND: The cause of podocyte injury in idiopathic nephrotic syndrome (INS) remains unknown. Although recent evidence points to the role of B cells and autoimmunity, the lack of animal models mediated by autoimmunity limits further research. We aimed to establish a mouse model mimicking human INS by immunizing mice with Crb2, a transmembrane protein expressed at the podocyte foot process. METHODS: C3H/HeN mice were immunized with the recombinant extracellular domain of mouse Crb2. Serum anti-Crb2 antibody, urine protein-to-creatinine ratio, and kidney histology were studied. For signaling studies, a Crb2-expressing mouse podocyte line was incubated with anti-Crb2 antibody. RESULTS: Serum anti-Crb2 autoantibodies and significant proteinuria were detected 4 weeks after the first immunization. The proteinuria reached nephrotic range at 9-13 weeks and persisted up to 29 weeks. Initial kidney histology resembled minimal change disease in humans, and immunofluorescence staining showed delicate punctate IgG staining in the glomerulus, which colocalized with Crb2 at the podocyte foot process. A subset of mice developed features resembling FSGS after 18 weeks. In glomeruli of immunized mice and in Crb2-expressing podocytes incubated with anti-Crb2 antibody, phosphorylation of ezrin, which connects Crb2 to the cytoskeleton, increased, accompanied by altered Crb2 localization and actin distribution. CONCLUSION: The results highlight the causative role of anti-Crb2 autoantibody in podocyte injury in mice. Crb2 immunization could be a useful model to study the immunologic pathogenesis of human INS, and may support the role of autoimmunity against podocyte proteins in INS.

Photocatalytic CO2 Reduction Using Water as an Electron Donor under Visible Light Irradiation by Z-Scheme and Photoelectrochemical Systems over (CuGa)0.5ZnS2 in the Presence of Basic Additives.

We demonstrated photocatalytic CO2 reduction using water as an electron donor under visible light irradiation by a Z-scheme photocatalyst and a photoelectrochemical cell using bare (CuGa)0.5ZnS2 prepared by a flux method as a CO2-reducing photocatalyst. The Z-scheme system employing the bare (CuGa)0.5ZnS2 photocatalyst and RGO-(CoOx/BiVO4) as an O2-evolving photocatalyst produced CO of a CO2 reduction product accompanied by H2 and O2 in a simple suspension system without any additives under visible light irradiation and 1 atm of CO2. When a basic salt (i.e., NaHCO3, NaOH, etc.) was added into the reactant solution (H2O + CO2), the CO formation rate and the CO selectivity increased. The same effect of the basic salt was observed for sacrificial CO2 reduction using SO32- as an electron donor over the bare (CuGa)0.5ZnS2 photocatalyst. The selectivity for the CO formation of the Z-schematic CO2 reduction reached 10-20% in the presence of the basic salt even in an aqueous solution and without loading any cocatalysts on the (CuGa)0.5ZnS2 metal sulfide photocatalyst. It is notable that CO was obtained accompanied by reasonable O2 evolution, indicating that water was an electron donor for the CO2 reduction. Moreover, the present Z-scheme system also showed activity for solar CO2 reduction using water as an electron donor. The bare (CuGa)0.5ZnS2 powder loaded on an FTO glass was also used as a photocathode for CO2 reduction under visible light irradiation. CO and H2 were obtained on the photocathode with 20% and 80% Faradaic efficiencies at 0.1 V vs RHE, respectively.

Genetic Ablation of Nrf2 Exacerbates Neuroinflammation in Ocular Autoimmunity.

Experimental autoimmune uveoretinitis (EAU) is an animal model of non-infectious uveitis and is developed by immunization with retinal antigen, interphotoreceptor retinoid-binding protein (IRBP). Nuclear factor erythroid 2- (NF-E2-) related factor 2 (Nrf2) is responsible for regulating antioxidant and inflammatory responses. In this study, we investigated the role of Nrf2 on the development of EAU. Clinical and pathological examination demonstrated that retinal inflammation was exacerbated in Nrf2 knockout (Nrf2 KO) mice compared to wild type (WT) mice, and the expression of inflammatory cytokines (IFN-γ, IL-6, and IL-17) in the retina was significantly elevated in Nrf2 KO mice. GFAP positive cells (astrocytes) and Iba-1 positive cells (microglia cells) in the retina were more numerous in Nrf2 KO mice compared to WT mice. Furthermore, we examined the suppressive effect of the Nrf2 activator CDDO-Im (2-cyano-3,12 dioxooleana-1,9 dien-28-oyl imidazoline) on the development of EAU. The treatment with CDDO-Im significantly reduced the clinical and pathological score of EAU compared to those of vehicle-treated mice. These findings suggest that Nrf2 plays a regulatory role in the pathogenesis of autoimmune uveoretinitis and the activation of the Nrf2 system may have therapeutic potential for protecting vision from autoimmune neuroinflammation.

Photocatalytic Overall Water Splitting Under Visible Light Enabled by a Particulate Conjugated Polymer Loaded with Palladium and Iridium.

Polymer photocatalysts have received growing attention in recent years for photocatalytic hydrogen production from water. Most studies report hydrogen production with sacrificial electron donors, which is unsuitable for large-scale hydrogen energy production. Here we show that the palladium/iridium oxide-loaded homopolymer of dibenzo[b,d]thiophene sulfone (P10) facilitates overall water splitting to produce stoichiometric amounts of H2 and O2 for an extended period (>60 hours) after the system stabilized. These results demonstrate that conjugated polymers can act as single component photocatalytic systems for overall water splitting when loaded with suitable co-catalysts, albeit currently with low activities. Transient spectroscopy shows that the IrO2 co-catalyst plays an important role in the generation of the charge separated state required for water splitting, with evidence for fast hole transfer to the co-catalyst.

GLCCI1 is a novel protector against glucocorticoid-induced apoptosis in T cells.

Glucocorticoids (GCs) potently induce T-cell apoptosis in a GC receptor (GR)-dependent manner and are used to control lymphocyte function in clinical practice. However, its downstream pathways remain controversial. Here, we showed that GC-induced transcript 1 (GLCCI1) is a novel downstream molecule of the GC-GR cascade that acts as an antiapoptotic mediator in thymic T cells. GLCCI1 was highly phosphorylated and colocalized with microtubules in GLCCI1-transfected human embryonic kidney QBI293A cells. GR-dependent up-regulation of GLCCI1 was associated with GC-induced proapoptotic events in a cultured thymocyte cell line. However, GLCCI1 knockdown in a thymocyte cell line led to apoptosis. Consistently, transgenic mice overexpressing human GLCCI1 displayed enlarged thymi that consisted of larger numbers of thymocytes. Further molecular characterization showed that GLCCI1 bound to both dynein light chain LC8-type 1 (LC8) and its functional kinase, p21-protein activated kinase 1 (PAK1), thereby inhibiting the kinase activity of PAK1 toward LC8 phosphorylation, a crucial event in apoptotic signaling. GLCCI1 induction facilitated LC8 dimer formation and reduced Bim expression. Thus, GLCCI1 is a candidate factor involved in apoptosis regulation of thymic T cells.-Kiuchi, Z., Nishibori, Y., Kutsuna, S., Kotani, M., Hada, I., Kimura, T., Fukutomi, T., Fukuhara, D., Ito-Nitta, N., Kudo, A., Takata, T., Ishigaki, Y., Tomosugi, N., Tanaka, H., Matsushima, S., Ogasawara, S., Hirayama, Y., Takematsu, H., Yan, K. GLCCI1 is a novel protector against glucocorticoid-induced apoptosis in T cells.

Mouse GTSF1 is an essential factor for secondary piRNA biogenesis.

The piRNA pathway is a piRNA-guided retrotransposon silencing system which includes processing of retrotransposon transcripts by PIWI-piRNAs in secondary piRNA biogenesis. Although several proteins participate in the piRNA pathway, the ones crucial for the cleavage of target RNAs by PIWI-piRNAs have not been identified. Here, we show that GTSF1, an essential factor for retrotransposon silencing in male germ cells in mice, associates with both MILI and MIWI2, mouse PIWI proteins that function in prospermatogonia. GTSF1 deficiency leads to a severe defect in the production of secondary piRNAs, which are generated from target RNAs of PIWI-piRNAs. Furthermore, in Gtsf1 mutants, a known target RNA of PIWI-piRNAs is left unsliced at the cleavage site, and the generation of secondary piRNAs from this transcript is defective. Our findings indicate that GTSF1 is a crucial factor for the slicing of target RNAs by PIWI-piRNAs and thus affects secondary piRNA biogenesis in prospermatogonia.

Template Synthesis of Well-Defined Rutile Nanoparticles by Solid-State Reaction at Room Temperature.

Well-defined nanoparticles of rutile (with the size of 5 nm) were successfully prepared by the unusual solid-state transformation of an amorphous precursor in well-defined nanospace of a mesoporous silica template (SBA-15) at room temperature. An aqueous colloidal suspension of the rutile nanoparticles was successfully obtained by dissolution of SBA-15 and subsequent pH adjustment. The isolated rutile nanoparticles were used for H2 evolution from an aqueous methanol solution by UV irradiation.

Activation of Water-Splitting Photocatalysts by Loading with Ultrafine Rh-Cr Mixed-Oxide Cocatalyst Nanoparticles.

The activity of many water-splitting photocatalysts could be improved by the use of RhIII -CrIII mixed oxide (Rh2-x Crx O3 ) particles as cocatalysts. Although further improvement of water-splitting activity could be achieved if the size of the Rh2-x Crx O3 particles was decreased further, it is difficult to load ultrafine (<2 nm) Rh2-x Crx O3 particles onto a photocatalyst by using conventional loading methods. In this study, a new loading method was successfully established and was used to load Rh2-x Crx O3 particles with a size of approximately 1.3 nm and a narrow size distribution onto a BaLa4 Ti4 O15 photocatalyst. The obtained photocatalyst exhibited an apparent quantum yield of 16 %, which is the highest achieved for BaLa4 Ti4 O15 to date. Thus, the developed loading technique of Rh2-x Crx O3 particles is extremely effective at improving the activity of the water-splitting photocatalyst BaLa4 Ti4 O15 . This method is expected to be extended to other advanced water-splitting photocatalysts to achieve higher quantum yields.

O-GlcNAcylation and phosphorylation of ß-actin Ser199 in diabetic nephropathy.

The function of actin is regulated by various posttranslational modifications. We have previously shown that in the kidneys of nonobese type 2 diabetes model Goto-Kakizaki rats, increased O-GlcNAcylation of ß-actin protein is observed. It has also been reported that both O-GlcNAcylation and phosphorylation occur on Ser199 of ß-actin. However, their roles are not known. To elucidate their roles in diabetic nephropathy, we examined the rat kidney for changes in O-GlcNAcylation of Ser199 (gS199)-actin and in the phosphorylation of Ser199 (pS199)-actin. Both gS199- and pS199-actin molecules had an apparent molecular weight of 40 kDa and were localized as nonfilamentous actin in both the cytoplasm and nucleus. Compared with the normal kidney, the immunostaining intensity of gS199-actin increased in podocytes of the glomeruli and in proximal tubules of the diabetic kidney, whereas that of pS199-actin did not change in podocytes but decreased in proximal tubules. We confirmed that the same results could be observed in the glomeruli of the human diabetic kidney. In podocytes of glomeruli cultured in the presence of the O-GlcNAcase inhibitor Thiamet G, increased O-GlcNAcylation was accompanied by a concomitant decrease in the amount of filamentous actin and in morphological changes. Our present results demonstrate that dysregulation of O-GlcNAcylation and phosphorylation of Ser199 occurred in diabetes, which may contribute partially to the causes of the morphological changes in the glomeruli and tubules. gS199- and pS199-actin will thus be useful for the pathological evaluation of diabetic nephropathy.

Z-scheme photocatalyst systems employing Rh- and Ir-doped metal oxide materials for water splitting under visible light irradiation.

Various types of Z-scheme systems for water splitting under visible light irradiation were successfully developed by employing Rh- and Ir-doped metal oxide powdered materials with relatively narrow energy gaps (EG): BaTa2O6:Ir,La (EG: 1.9-2.0 eV), NaTaO3:Ir,La (EG: 2.1-2.3 eV), SrTiO3:Ir (EG: 1.6-1.8 eV), NaNbO3:Rh,Ba (EG: 2.5 eV) and TiO2:Rh,Sb (EG: 2.1 eV), with conventional SrTiO3:Rh (an H2-evolving photocatalyst) or BiVO4 (an O2-evolving photocatalyst), and suitable electron mediators. The Z-scheme systems were classified into three groups depending on the combination of H2- and O2-evolving photocatalysts and electron mediator. The Z-scheme systems combining BaTa2O6:Ir,La with BiVO4, and NaTaO3:Ir,La with BiVO4 were active when a [Co(bpy)3]3+/2+ redox couple was used rather than an Fe3+/2+ one. The combination of SrTiO3:Ir with SrTiO3:Rh gave an activity when the [Co(bpy)3]3+/2+ and Fe3+/2+ redox couple ionic mediators were used. The Z-scheme systems combining NaNbO3:Rh,Ba and TiO2:Rh,Sb with SrTiO3:Rh showed activities by using the [Co(bpy)3]3+/2+ and Fe3+/2+ redox couples and also via interparticle electron transfer by just contact with/without reduced graphene oxide (RGO). These suitable combinations can be explained based on the impurity levels of doped Rh3+ and Ir3+ toward the redox potentials of the ionic mediators for the Z-scheme systems employing ionic mediators, and p-/n-type and onset potentials of the photocurrent in the photoelectrochemical properties of those photocatalyst materials for the Z-scheme systems working via interparticle electron transfer.

Particulate Photocatalyst Sheets Based on Carbon Conductor Layer for Efficient Z-Scheme Pure-Water Splitting at Ambient Pressure.

Development of sunlight-driven water splitting systems with high efficiency, scalability, and cost-competitiveness is a central issue for mass production of solar hydrogen as a renewable and storable energy carrier. Photocatalyst sheets comprising a particulate hydrogen evolution photocatalyst (HEP) and an oxygen evolution photocatalyst (OEP) embedded in a conductive thin film can realize efficient and scalable solar hydrogen production using Z-scheme water splitting. However, the use of expensive precious metal thin films that also promote reverse reactions is a major obstacle to developing a cost-effective process at ambient pressure. In this study, we present a standalone particulate photocatalyst sheet based on an earth-abundant, relatively inert, and conductive carbon film for efficient Z-scheme water splitting at ambient pressure. A SrTiO3:La,Rh/C/BiVO4:Mo sheet is shown to achieve unassisted pure-water (pH 6.8) splitting with a solar-to-hydrogen energy conversion efficiency (STH) of 1.2% at 331 K and 10 kPa, while retaining 80% of this efficiency at 91 kPa. The STH value of 1.0% is the highest among Z-scheme pure water splitting operating at ambient pressure. The working mechanism of the photocatalyst sheet is discussed on the basis of band diagram simulation. In addition, the photocatalyst sheet split pure water more efficiently than conventional powder suspension systems and photoelectrochemical parallel cells because H+ and OH- concentration overpotentials and an IR drop between the HEP and OEP were effectively suppressed. The proposed carbon-based photocatalyst sheet, which can be used at ambient pressure, is an important alternative to (photo)electrochemical systems for practical solar hydrogen production.

Wolf-Hirschhorn syndrome candidate 1-like 1 epigenetically regulates nephrin gene expression.

Altered expression of nephrin underlies the pathophysiology of proteinuria in both congenital and acquired nephrotic syndrome. However, the epigenetic mechanisms of nephrin gene regulation remain elusive. Here, we show that Wolf-Hirschhorn syndrome candidate 1-like 1 long form (WHSC1L1-L) is a novel epigenetic modifier of nephrin gene regulation. WHSC1L1-L was associated with histone H3K4 and H3K36 in human embryonic kidney cells. WHSC1L1-L gene was expressed in the podocytes, and functional protein product was detected in these cells. WHSC1L1-L was found to bind nephrin but not other podocyte-specific gene promoters, leading to its inhibition/suppression, abrogating the stimulatory effect of WT1 and NF-κB. Gene knockdown of WHSC1L1-L in primary cultured podocytes accelerated the transcription of nephrin but not CD2AP. An in vivo zebrafish study involving the injection of Whsc1l1 mRNA into embryos demonstrated an apparent reduction of nephrin mRNA but not podocin and CD2AP mRNA. Immunohistochemistry showed that both WHSC1L1-L and nephrin emerged at the S-shaped body stage in glomeruli. Immunofluorescence and confocal microscopy displayed WHSC1L1 to colocalize with trimethylated H3K4 in the glomerular podocytes. Chromatin immunoprecipitation assay revealed the reduction of the association of trimethylated H3K4 at the nephrin promoter regions. Finally, nephrin mRNA was upregulated in the glomerulus at the early proteinuric stage of mouse nephrosis, which was associated with the reduction of WHSC1L1. In conclusion, our results demonstrate that WHSC1L1-L acts as a histone methyltransferase in podocytes and regulates nephrin gene expression, which may in turn contribute to the integrity of the slit diaphragm of the glomerular filtration barrier.