Going with the flow: analysis of population structure reveals high gene flow shaping invasion pattern and inducing range expansion of Mikania micrantha in Asia.

BACKGROUND AND AIMS: Mikania micrantha, a climbing perennial weed of the family Asteraceae, is native to Latin America and is highly invasive in the tropical belt of Asia, Oceania and Australia. This study was framed to investigate the population structure of M. micrantha at a large spatial scale in Asia and to identify how introduction history, evolutionary forces and landscape features influenced the genetic pattern of the species in this region. METHODS: We assessed the genetic diversity and structure of 1052 individuals from 46 populations for 12 microsatellite loci. The spatial pattern of genetic variation was investigated by estimating the relationship between genetic distance and geographical, climatic and landscape resistances hypothesized to influence gene flow between populations. KEY RESULTS: We found high genetic diversity of M. micrantha in this region, as compared with the genetic diversity parameters of other invasive species. Spatial and non-spatial clustering algorithms identified the presence of multiple genetic clusters and admixture between populations. Most of the populations showed heterozygote deficiency, primarily due to inbreeding, and the founder populations showed evidence of a genetic bottleneck. Persistent gene flow throughout the invasive range caused low genetic differentiation among populations and provided beneficial genetic variation to the marginal populations in a heterogeneous environment. Environmental suitability was found to buffer the detrimental effects of inbreeding at the leading edge of range expansion. Both linear and non-linear regression models demonstrated a weak relationship between genetic distance and geographical distance, as well as bioclimatic variables and environmental resistance surfaces. CONCLUSIONS: These findings provide evidence that extensive gene flow and admixture between populations have influenced the current genetic pattern of M. micrantha in this region. High gene flow across the invaded landscape may facilitate adaptation, establishment and long-term persistence of the population, thereby indicating the range expansion ability of the species.

Fundamental understanding of nitrogen in biomass electrocatalysts for oxygen reduction and zinc-air batteries.

Exploring high-efficiency catalysts for oxygen reduction reactions (ORRs) is essential for the development of large-scale applications of fuel cell and metal-air batteries technology. The as-prepared Fe-NC-800 via polymerization-pyrolysis strategy exhibited superior ORR activity with onset potential of 1.030 V vs. reversible hydrogen electrode (RHE) and half-wave potential of 0.908 V vs. RHE, which is higher than that of the Pt/C catalyst and most of other Fe-based catalysts. The different d-band center values can be attributed to the influence of different N-doped carbon, leading to the adjustment in the ORR activity. In addition, Fe-NC-800-based Zn-air battery showed better electrochemical performance with a high discharge specific capacity of 806 mA h g-1 and a high-power density of 220 mW cm-2 than that of the Pt/C-based battery. Therefore, the biomass Fe-NC-800 catalyst may become a promising substitute for Pt/C catalysts in energy storage and conversion devices.