RESUMO
The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. oryzae BCC7051. The obtained reads were de novo assembled into 25 scaffolds spanning of 38,550,958 bps with predicted 11,456 protein-coding genes. By synteny mapping, a large rearrangement was found in two scaffolds of A. oryzae BCC7051 as compared to the reference RIB40 strain. The genetic relationship between BCC7051 and other strains of A. oryzae in terms of aflatoxin production was investigated, indicating that the A. oryzae BCC7051 was categorized into group 2 nonaflatoxin-producing strain. Moreover, a comparative analysis of the structural genes focusing on the involvement in lipid metabolism among oleaginous yeast and fungi revealed the presence of multiple isoforms of metabolic enzymes responsible for fatty acid synthesis in BCC7051. The alternative routes of acetyl-CoA generation as oleaginous features and malate/citrate/pyruvate shuttle were also identified in this A. oryzae strain. The genome sequence generated in this work is a dedicated resource for expanding genome-wide study of microbial lipids at systems level, and developing the fungal-based platform for production of diversified lipids with commercial relevance.
Assuntos
Aspergillus oryzae/genética , Aspergillus oryzae/metabolismo , Genoma Fúngico , Lipídeos/biossíntese , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Malatos/metabolismo , SinteniaRESUMO
Understanding the evolution of the free-living, cyanobacterial, diazotroph Trichodesmium is of great importance because of its critical role in oceanic biogeochemistry and primary production. Unlike the other >150 available genomes of free-living cyanobacteria, only 63.8% of the Trichodesmium erythraeum (strain IMS101) genome is predicted to encode protein, which is 20-25% less than the average for other cyanobacteria and nonpathogenic, free-living bacteria. We use distinctive isolates and metagenomic data to show that low coding density observed in IMS101 is a common feature of the Trichodesmium genus, both in culture and in situ. Transcriptome analysis indicates that 86% of the noncoding space is expressed, although the function of these transcripts is unclear. The density of noncoding, possible regulatory elements predicted in Trichodesmium, when normalized per intergenic kilobase, was comparable and twofold higher than that found in the gene-dense genomes of the sympatric cyanobacterial genera Synechococcus and Prochlorococcus, respectively. Conserved Trichodesmium noncoding RNA secondary structures were predicted between most culture and metagenomic sequences, lending support to the structural conservation. Conservation of these intergenic regions in spatiotemporally separated Trichodesmium populations suggests possible genus-wide selection for their maintenance. These large intergenic spacers may have developed during intervals of strong genetic drift caused by periodic blooms of a subset of genotypes, which may have reduced effective population size. Our data suggest that transposition of selfish DNA, low effective population size, and high-fidelity replication allowed the unusual "inflation" of noncoding sequence observed in Trichodesmium despite its oligotrophic lifestyle.
Assuntos
Cianobactérias/genética , Cianobactérias/fisiologia , DNA Bacteriano/química , Proteínas de Bactérias/química , Carbono/química , Biologia Computacional , DNA Bacteriano/genética , DNA Intergênico/genética , Ecossistema , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Genoma , Genômica , Dados de Sequência Molecular , Nitrogênio/química , Fixação de Nitrogênio/genética , Conformação de Ácido Nucleico , Oceanos e Mares , Prochlorococcus/genética , RNA/química , RNA/genética , Transdução de Sinais , Synechococcus/genética , Transposases/metabolismoRESUMO
UNLABELLED: Bacterial endophytes that colonize Populus trees contribute to nutrient acquisition, prime immunity responses, and directly or indirectly increase both above- and below-ground biomasses. Endophytes are embedded within plant material, so physical separation and isolation are difficult tasks. Application of culture-independent methods, such as metagenome or bacterial transcriptome sequencing, has been limited due to the predominance of DNA from the plant biomass. Here, we describe a modified differential and density gradient centrifugation-based protocol for the separation of endophytic bacteria from Populus roots. This protocol achieved substantial reduction in contaminating plant DNA, allowed enrichment of endophytic bacteria away from the plant material, and enabled single-cell genomics analysis. Four single-cell genomes were selected for whole-genome amplification based on their rarity in the microbiome (potentially uncultured taxa) as well as their inferred abilities to form associations with plants. Bioinformatics analyses, including assembly, contamination removal, and completeness estimation, were performed to obtain single-amplified genomes (SAGs) of organisms from the phyla Armatimonadetes, Verrucomicrobia, and Planctomycetes, which were unrepresented in our previous cultivation efforts. Comparative genomic analysis revealed unique characteristics of each SAG that could facilitate future cultivation efforts for these bacteria. IMPORTANCE: Plant roots harbor a diverse collection of microbes that live within host tissues. To gain a comprehensive understanding of microbial adaptations to this endophytic lifestyle from strains that cannot be cultivated, it is necessary to separate bacterial cells from the predominance of plant tissue. This study provides a valuable approach for the separation and isolation of endophytic bacteria from plant root tissue. Isolated live bacteria provide material for microbiome sequencing, single-cell genomics, and analyses of genomes of uncultured bacteria to provide genomics information that will facilitate future cultivation attempts.
Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Endófitos/classificação , Endófitos/isolamento & purificação , Raízes de Plantas/microbiologia , Populus/microbiologia , Bactérias/genética , Centrifugação com Gradiente de Concentração/métodos , Biologia Computacional , Endófitos/genética , Metagenômica , Análise de Sequência de DNA , Análise de Célula Única/métodosRESUMO
MOTIVATION: To assess the potential of different types of sequence data combined with de novo and hybrid assembly approaches to improve existing draft genome sequences. RESULTS: Illumina, 454 and PacBio sequencing technologies were used to generate de novo and hybrid genome assemblies for four different bacteria, which were assessed for quality using summary statistics (e.g. number of contigs, N50) and in silico evaluation tools. Differences in predictions of multiple copies of rDNA operons for each respective bacterium were evaluated by PCR and Sanger sequencing, and then the validated results were applied as an additional criterion to rank assemblies. In general, assemblies using longer PacBio reads were better able to resolve repetitive regions. In this study, the combination of Illumina and PacBio sequence data assembled through the ALLPATHS-LG algorithm gave the best summary statistics and most accurate rDNA operon number predictions. This study will aid others looking to improve existing draft genome assemblies. AVAILABILITY AND IMPLEMENTATION: All assembly tools except CLC Genomics Workbench are freely available under GNU General Public License. CONTACT: brownsd@ornl.gov SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Assuntos
Biologia Computacional/métodos , Genômica/métodos , Análise de Sequência de DNA/métodos , Algoritmos , Sequência de Bases , Mapeamento de Sequências Contíguas , DNA Bacteriano/análise , DNA Ribossômico/química , Reprodutibilidade dos TestesRESUMO
Clostridium thermocellum is a thermophilic, obligately anaerobic, gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing. Ethanol intolerance is an important metric in terms of process economics, and tolerance has often been described as a complex and likely multigenic trait for which complex gene interactions come into play. Here, we resequence the genome of an ethanol-tolerant mutant, show that the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), hypothesize based on structural analysis that cofactor specificity may be affected, and confirm this hypothesis using enzyme assays. Biochemical assays confirm a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity, which likely affects electron flow in the mutant. The simplicity of the genetic basis for the ethanol-tolerant phenotype observed here informs rational engineering of mutant microbial strains for cellulosic ethanol production.
Assuntos
Álcool Desidrogenase/genética , Clostridium thermocellum/genética , Tolerância a Medicamentos/genética , Etanol/metabolismo , Mutação , Aldeído Oxirredutases , Clostridium thermocellum/enzimologia , Clostridium thermocellum/fisiologia , NAD , NADPRESUMO
The application of systems biology tools holds promise for rational industrial microbial strain development. Here, we characterize a Zymomonas mobilis mutant (AcR) demonstrating sodium acetate tolerance that has potential importance in biofuel development. The genome changes associated with AcR are determined using microarray comparative genome sequencing (CGS) and 454-pyrosequencing. Sanger sequencing analysis is employed to validate genomic differences and to investigate CGS and 454-pyrosequencing limitations. Transcriptomics, genetic data and growth studies indicate that over-expression of the sodium-proton antiporter gene nhaA confers the elevated AcR sodium acetate tolerance phenotype. nhaA over-expression mostly confers enhanced sodium (Na(+)) tolerance and not acetate (Ac(-)) tolerance, unless both ions are present in sufficient quantities. NaAc is more inhibitory than potassium and ammonium acetate for Z. mobilis and the combination of elevated Na(+) and Ac(-) ions exerts a synergistic inhibitory effect for strain ZM4. A structural model for the NhaA sodium-proton antiporter is constructed to provide mechanistic insights. We demonstrate that Saccharomyces cerevisiae sodium-proton antiporter genes also contribute to sodium acetate, potassium acetate, and ammonium acetate tolerances. The present combination of classical and systems biology tools is a paradigm for accelerated industrial strain improvement and combines benefits of few a priori assumptions with detailed, rapid, mechanistic studies.
Assuntos
Loci Gênicos , Saccharomyces cerevisiae/genética , Acetato de Sódio/metabolismo , Zymomonas/genética , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Regulação Fúngica da Expressão Gênica , Engenharia Genética , Genoma Bacteriano , Modelos Moleculares , Mutação , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/metabolismo , Trocadores de Sódio-Hidrogênio/química , Trocadores de Sódio-Hidrogênio/genética , Trocadores de Sódio-Hidrogênio/metabolismo , Zymomonas/química , Zymomonas/metabolismoRESUMO
Metagenomics has provided access to genomes of as yet uncultivated microorganisms in natural environments, yet there are gaps in our knowledge-particularly for Archaea-that occur at relatively low abundance and in extreme environments. Ultrasmall cells (<500 nm in diameter) from lineages without cultivated representatives that branch near the crenarchaeal/euryarchaeal divide have been detected in a variety of acidic ecosystems. We reconstructed composite, near-complete approximately 1-Mb genomes for three lineages, referred to as ARMAN (archaeal Richmond Mine acidophilic nanoorganisms), from environmental samples and a biofilm filtrate. Genes of two lineages are among the smallest yet described, enabling a 10% higher coding density than found genomes of the same size, and there are noncontiguous genes. No biological function could be inferred for up to 45% of genes and no more than 63% of the predicted proteins could be assigned to a revised set of archaeal clusters of orthologous groups. Some core metabolic genes are more common in Crenarchaeota than Euryarchaeota, up to 21% of genes have the highest sequence identity to bacterial genes, and 12 belong to clusters of orthologous groups that were previously exclusive to bacteria. A small subset of 3D cryo-electron tomographic reconstructions clearly show penetration of the ARMAN cell wall and cytoplasmic membranes by protuberances extended from cells of the archaeal order Thermoplasmatales. Interspecies interactions, the presence of a unique internal tubular organelle [Comolli, et al. (2009) ISME J 3:159-167], and many genes previously only affiliated with Crenarchaea or Bacteria indicate extensive unique physiology in organisms that branched close to the time that Cren- and Euryarchaeotal lineages diverged.
Assuntos
Archaea/citologia , Archaea/genética , Archaea/metabolismo , Archaea/ultraestrutura , Proteínas Arqueais/classificação , Proteínas Arqueais/genética , Biofilmes , Ciclo Celular , Replicação do DNA , Genoma Arqueal/genética , Genoma Bacteriano/genética , Dados de Sequência Molecular , Biossíntese de Proteínas , Proteômica , Especificidade da Espécie , Transcrição GênicaRESUMO
Uncultivated Bacteria and Archaea account for the vast majority of species on Earth, but obtaining their genomes directly from the environment, using shotgun sequencing, has only become possible recently. To realize the hope of capturing Earth's microbial genetic complement and to facilitate the investigation of the functional roles of specific lineages in a given ecosystem, technologies that accelerate the recovery of high-quality genomes are necessary. We present a series of analysis steps and data products for the extraction of high-quality metagenome-assembled genomes (MAGs) from microbiomes using the U.S. Department of Energy Systems Biology Knowledgebase (KBase) platform ( http://www.kbase.us/ ). Overall, these steps take about a day to obtain extracted genomes when starting from smaller environmental shotgun read libraries, or up to about a week from larger libraries. In KBase, the process is end-to-end, allowing a user to go from the initial sequencing reads all the way through to MAGs, which can then be analyzed with other KBase capabilities such as phylogenetic placement, functional assignment, metabolic modeling, pangenome functional profiling, RNA-Seq and others. While portions of such capabilities are available individually from other resources, the combination of the intuitive usability, data interoperability and integration of tools in a freely available computational resource makes KBase a powerful platform for obtaining MAGs from microbiomes. While this workflow offers tools for each of the key steps in the genome extraction process, it also provides a scaffold that can be easily extended with additional MAG recovery and analysis tools, via the KBase software development kit (SDK).
Assuntos
Metagenoma , Microbiota , Filogenia , Genoma Bacteriano , Microbiota/genética , Bactérias/genética , MetagenômicaRESUMO
To aid in the investigation of the Populus deltoides microbiome, we generated draft genome sequences for 21 Pseudomonas strains and 19 other diverse bacteria isolated from Populus deltoides roots. Genome sequences for isolates similar to Acidovorax, Bradyrhizobium, Brevibacillus, Caulobacter, Chryseobacterium, Flavobacterium, Herbaspirillum, Novosphingobium, Pantoea, Phyllobacterium, Polaromonas, Rhizobium, Sphingobium, and Variovorax were generated.
Assuntos
Bactérias/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Bactérias/isolamento & purificação , Endófitos/genética , Endófitos/isolamento & purificação , Metagenoma , Dados de Sequência Molecular , Raízes de Plantas/microbiologia , Populus/microbiologia , Rizosfera , Microbiologia do SoloRESUMO
Rahnella aquatilis CIP 78.65 is a gammaproteobacterium isolated from a drinking water source in Lille, France. Here we report the complete genome sequence of Rahnella aquatilis CIP 78.65, the type strain of R. aquatilis.
Assuntos
Água Potável/microbiologia , Genoma Bacteriano , Rahnella/genética , Sequência de Bases , França , Dados de Sequência Molecular , Rahnella/classificação , Rahnella/isolamento & purificaçãoRESUMO
Rahnella sp. strain Y9602 is a gammaproteobacterium isolated from contaminated subsurface soils that is capable of promoting uranium phosphate mineralization as a result of constitutive phosphatase activity. Here we report the first complete genome sequence of an isolate belonging to the genus Rahnella.
Assuntos
Genoma Bacteriano , Metais/química , Radioisótopos/química , Rahnella/genética , Microbiologia do Solo , Sequência de Bases , Dados de Sequência Molecular , Rahnella/classificação , Poluentes do Solo/químicaRESUMO
Microbacterium laevaniformans strain OR221 was isolated from subsurface sediments obtained from the Field Research Center (FRC) in Oak Ridge, TN. It was characterized as a bacterium tolerant to heavy metals, such as uranium, nickel, cobalt, and cadmium, as well as nitrate and low pH. We present its draft genome sequence.
Assuntos
Actinomycetales/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Actinomycetales/efeitos dos fármacos , Actinomycetales/isolamento & purificação , Tolerância a Medicamentos , Microbiologia Ambiental , Concentração de Íons de Hidrogênio , Metais Pesados/toxicidade , Dados de Sequência Molecular , Nitratos/toxicidade , Análise de Sequência de DNA , TennesseeRESUMO
Clostridium thermocellum wild-type strain YS is an anaerobic, thermophilic, cellulolytic bacterium capable of directly converting cellulosic substrates into ethanol. Strain YS and a derived cellulose adhesion-defective mutant strain, AD2, played pivotal roles in describing the original cellulosome concept. We present their draft genome sequences.
Assuntos
Clostridium thermocellum/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Aderência Bacteriana , Celulose/metabolismo , Clostridium thermocellum/metabolismo , Clostridium thermocellum/fisiologia , Etanol/metabolismo , Dados de Sequência Molecular , Mutação , Análise de Sequência de DNARESUMO
Rhizobium sp. strain PDO1-076 is a plant-associated bacterium isolated from Populus deltoides, and its draft genome sequence is reported.
Assuntos
Genoma Bacteriano , Populus/microbiologia , Rhizobium/genética , Rhizobium/isolamento & purificação , Cromossomos Bacterianos , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência MolecularRESUMO
We announce the availability of a high-quality draft of the genome sequence of Amycolatopsis sp. strain 39116, one of few bacterial species that are known to consume the lignin component of plant biomass. This genome sequence will further ongoing efforts to use microorganisms for the conversion of plant biomass into fuels and high-value chemicals.
Assuntos
Actinobacteria/classificação , Actinobacteria/genética , Biomassa , Genoma Bacteriano , Plantas , Actinobacteria/metabolismo , Biodegradação Ambiental , Cromossomos Bacterianos , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência MolecularRESUMO
Microbial communities in the termite hindgut are essential for degrading plant material. We present the high-quality draft genome sequence of the Opitutaceae bacterium strain TAV1, the first member of the phylum Verrucomicrobia to be isolated from wood-feeding termites. The genomic analysis reveals genes coding for lignocellulosic degradation and nitrogen fixation.
Assuntos
Genoma Bacteriano , Isópteros/microbiologia , Simbiose/fisiologia , Verrucomicrobia/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Dados de Sequência MolecularRESUMO
Pelosinus fermentans 16S rRNA gene sequences have been reported from diverse geographical sites since the recent isolation of the type strain. We present the genome sequence of the P. fermentans type strain R7 (DSM 17108) and genome sequences for two new strains with different abilities to reduce iron, chromate, and uranium.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Veillonellaceae/genética , Cromo/metabolismo , Microbiologia Ambiental , Poluentes Ambientais/metabolismo , Ferro/metabolismo , Dados de Sequência Molecular , Oxirredução , Urânio/metabolismo , Veillonellaceae/isolamento & purificação , Veillonellaceae/metabolismoRESUMO
Extremely thermophilic bacteria of the genus Caldicellulosiruptor utilize carbohydrate components of plant cell walls, including cellulose and hemicellulose, facilitated by a diverse set of glycoside hydrolases (GHs). From a biofuel perspective, this capability is crucial for deconstruction of plant biomass into fermentable sugars. While all species from the genus grow on xylan and acid-pretreated switchgrass, growth on crystalline cellulose is variable. The basis for this variability was examined using microbiological, genomic, and proteomic analyses of eight globally diverse Caldicellulosiruptor species. The open Caldicellulosiruptor pangenome (4,009 open reading frames [ORFs]) encodes 106 GHs, representing 43 GH families, but only 26 GHs from 17 families are included in the core (noncellulosic) genome (1,543 ORFs). Differentiating the strongly cellulolytic Caldicellulosiruptor species from the others is a specific genomic locus that encodes multidomain cellulases from GH families 9 and 48, which are associated with cellulose-binding modules. This locus also encodes a novel adhesin associated with type IV pili, which was identified in the exoproteome bound to crystalline cellulose. Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemicellulose.
Assuntos
Biomassa , Metabolismo dos Carboidratos , Celulose/metabolismo , Bactérias Gram-Positivas/genética , Redes e Vias Metabólicas/genética , Plantas/química , Adesinas Bacterianas/análise , Adesinas Bacterianas/genética , Celulases/análise , Celulases/genética , Variação Genética , Genoma Bacteriano , Bactérias Gram-Positivas/enzimologia , Proteoma/análiseRESUMO
Desulfurococcus fermentans is the first known cellulolytic archaeon. This hyperthermophilic and strictly anaerobic crenarchaeon produces hydrogen from fermentation of various carbohydrates and peptides without inhibition by accumulating hydrogen. The complete genome sequence reported here suggested that D. fermentans employs membrane-bound hydrogenases and novel glycohydrolases for hydrogen production from cellulose.
Assuntos
DNA Arqueal/química , DNA Arqueal/genética , Desulfurococcaceae/genética , Genoma Arqueal , Análise de Sequência de DNA , Anaerobiose , Metabolismo dos Carboidratos , Celulose/metabolismo , Desulfurococcaceae/isolamento & purificação , Desulfurococcaceae/fisiologia , Fermentação , Água Doce/microbiologia , Fontes Termais/microbiologia , Hidrogênio/metabolismo , Dados de Sequência Molecular , Federação RussaRESUMO
The complete and assembled genome sequences were determined for six strains of the alphaproteobacterial genus Methylobacterium, chosen for their key adaptations to different plant-associated niches and environmental constraints.