RESUMO
Mathematical models taking temperature variations into account are useful in predicting microbial growth in foods, like meat products, for which Lactobacillus plantarum is a mesophilic and one of the main spoiling bacterium. The current study assessed the ability of the main primary models and their non-isothermal versions to predict L. plantarum growth under constant and variable temperature. Experimental data of microbial growth were obtained in MRS medium under isothermal conditions (4, 8, 12, 16, 20, and 30°C) which were used to obtain the secondary models. The experimental data under non-isothermal conditions (periodically oscillating temperature between the plateaus 4-12, 5-15, and 20-30°C) were used to validate the non-isothermal models. The bias factors indicated that all assessed models provided safe predictions of the microorganism growth at the non-isothermal conditions. Overall, despite the very good performance of the primary models (isothermal), none of the models was able to predict with accuracy the L. plantarum growth under temperature variations, mainly when the temperature range was close to refrigeration temperature. Incorporating the complex microbial adaptation mechanisms into the predictive models is a challenge to be overcome.
Assuntos
Fenômenos Fisiológicos Bacterianos , Lactobacillus plantarum/crescimento & desenvolvimento , Modelos BiológicosRESUMO
The aim of this study was to produce thermoplastic starch (TPS) films and to enhance their properties by reinforcing them with hydrophilic and hydrophobic nanoclays. TPS films were prepared by extrusion and thermopressing, and their crystallinity, water vapor permeability (WVP), and mechanical properties were studied. The hydrophilic nanoclay lowered the material WVP due to the formation of an intercalated composite. The hydrophobic nanoclays increased the rigidity of the films but did not alter the tensile strength. The blending of nanoclays with thermoplastic starch modifies the mechanical properties and WVP, and these changes are strongly associated with the dispersion of nanoclay in the polymer matrix. The dispersion, in turn, depends on the compatibility of the matrix and the nanoclay in terms of the hygroscopicity and the concentration in which the nanoclay is used. The addition of nanoclays to starch-based films is a promising way to enhance them for industrial manufacture.
RESUMO
"Canned, frozen, processed, ultra-processed, functional" etc. Two hundred years after the beginning of the food industry, industrialized food has evolved with many labels. Every person in the world eats and has different experiences with food that are connected to culture and social relationships which permeate our daily lives in many kinds of situations. Food evokes feelings, beliefs, desires, and moral values. For many people, food not only satisfies hunger and sustains life, but it also brings a delicious pleasure that is with their history, culture, and ancestry. Today's food industry pushes products through its marketing, which promotes a plethora of claims that have now trended proportionally with neophobic dimensions. In reality, the general public lacks objective knowledge about the complex science of modern food technology because of its low transparency, and this has resulted in the appearance of misleading ideas that can prejudice the correct analysis of food values. Given this, education about food is an urgent need. Notably, food scientists, technologists, and engineers must look at eaters through the prism of consumers who are human beings in all their rich social/anthropological diversity. The objective of this article is to explore the elemental anthropologic aspects of foods and how they can affect consumer's trust in the food industry's role.
RESUMO
Protein from plant sources is claimed alternatives to animal sources in the human diet. Suitable protein sources need high protein digestibility and amino acid bioavailability. In terms of protein functionality and food applications, they also need high-quality attributes, such as solubility, gelling, water- and oil-holding capacities, emulsifying, and foaming. Thermal processing can improve the nutritional quality of plants with some disadvantages, like reducing the assimilation of micronutrients (vitamins and minerals). Emerging technologies-such as ultrasound, high-pressure, ohmic heating, microwave, pulsed electric field, cold plasma, and enzymatic processes-can overcome those disadvantages. Recent studies demonstrate their enormous potential to improve protein techno-functional properties, protein quality, and decrease protein allergenicity. However, the literature lacks a broader evaluation, including protein digestibility, industrial-scale optimization, and exploring applications to these alternative protein sources.
RESUMO
Native potato starch has a semi-crystalline structure associated with a low glycemic index. Microwave vacuum drying (MWVD) produces crispy snacks under mild temperatures, reducing starch structural changes. However, blanching pretreatment gelatinizes starch, reducing crystallinity. A promising alternative is drying raw or blanched-then-cooled potatoes by MWVD. Cooling the blanched potato before MWVD aims to promote the partial return of the crystalline structure. Thus, this study evaluated how different pretreatments affect potato chips' starch structure and physical properties. Three samples were dried by MWVD: (i) raw (MWVD-RW), (ii) blanched (MWVD-BL), and (iii) blanched followed by cooling (4 °C for 48 h) (MWVD-BLC) potatoes. MWVD-RW samples presented a higher starch crystallinity (16.9%), which disappeared in MWVD-BL samples and partially returned in MWVD-BLC (8.7%). MWVD-BL and MWVD-BLC samples presented lower bulk (<0.338 g cm−3) density and higher porosity (>74%) and crispness. On the other hand, MWVD-BLC samples presented intermediate characteristics for color, true density, and porous distribution compared to others. All samples showed high porosity (>69%) and crispy texture. Therefore, based on the quality assessment, the MWVD-RW and MWVD-BLC produced healthy and crispy oil-free chips with a potentially lower glycemic index.
RESUMO
Color change of fruit-based products during storage is an important quality parameter to determine their shelf life. In this study, a combination of relative humidity (RH) and illumination was evaluated on the stability of strawberry leathers. Samples were conditioned at 25 °C, in chambers with RH of 22.5% and 52.3% and under two levels of illumination (no illumination and with a light-emitting diode (LED) illumination at 1010 lx). Samples were analyzed during storage by instrumental color measurements, total anthocyanin content, and consumers' acceptance/rejection of the product color. Current-status survival analysis was performed to estimate the sensory-based shelf-life of the strawberry leather. The chromatic parameters (a* and ΔE* values) and anthocyanin content changed with increasing storage time and RH, fitting a first-order fractional conversion model. Samples conditioned at the higher RH showed a higher reduction of a* values and anthocyanins losses when stored under LED illumination than those without illumination. The increase of RH resulted in a faster increase of the consumer rejection probability and a shorter shelf life of the strawberry leather. For 50% of consumers' rejection, the sensory shelf life of the strawberry leather equilibrated at 22.5% RH was estimated as at least 54 days, while it was reduced to approximately 2 days at 52.3% RH. The red chromatic parameter (a* value) strongly correlated to the percentage of consumer rejection in all storage conditions, suggesting that this analytical parameter can be useful as a predictor of strawberry leather's shelf life. Therefore, the results of this study show the applicability of an approach that integrates instrumental and sensory data to acquire faster information on color changes during the storage of strawberry leather and product shelf-life prediction.
RESUMO
In predictive microbiology, the model parameters have been estimated using the sequential two-step modeling (TSM) approach, in which primary models are fitted to the microbial growth data, and then secondary models are fitted to the primary model parameters to represent their dependence with the environmental variables (e.g., temperature). The Optimal Experimental Design (OED) approach allows reducing the experimental workload and costs, and the improvement of model identifiability because primary and secondary models are fitted simultaneously from non-isothermal data. Lactobacillus viridescens was selected to this study because it is a lactic acid bacterium of great interest to meat products preservation. The objectives of this study were to estimate the growth parameters of L. viridescens in culture medium from TSM and OED approaches and to evaluate both the number of experimental data and the time needed in each approach and the confidence intervals of the model parameters. Experimental data for estimating the model parameters with TSM approach were obtained at six temperatures (total experimental time of 3540h and 196 experimental data of microbial growth). Data for OED approach were obtained from four optimal non-isothermal profiles (total experimental time of 588h and 60 experimental data of microbial growth), two profiles with increasing temperatures (IT) and two with decreasing temperatures (DT). The Baranyi and Roberts primary model and the square root secondary model were used to describe the microbial growth, in which the parameters b and Tmin (±95% confidence interval) were estimated from the experimental data. The parameters obtained from TSM approach were b=0.0290 (±0.0020) [1/(h0.5°C)] and Tmin=-1.33 (±1.26) [°C], with R2=0.986 and RMSE=0.581, and the parameters obtained with the OED approach were b=0.0316 (±0.0013) [1/(h0.5°C)] and Tmin=-0.24 (±0.55) [°C], with R2=0.990 and RMSE=0.436. The parameters obtained from OED approach presented smaller confidence intervals and best statistical indexes than those from TSM approach. Besides, less experimental data and time were needed to estimate the model parameters with OED than TSM. Furthermore, the OED model parameters were validated with non-isothermal experimental data with great accuracy. In this way, OED approach is feasible and is a very useful tool to improve the prediction of microbial growth under non-isothermal condition.
Assuntos
Microbiologia de Alimentos/métodos , Conservação de Alimentos , Armazenamento de Alimentos , Lactobacillus/crescimento & desenvolvimento , Produtos da Carne/microbiologia , Modelos Biológicos , Contagem de Colônia Microbiana , Cinética , Modelos Teóricos , Projetos de Pesquisa , TemperaturaRESUMO
Lactic acid bacteria (LAB) are responsible for spoiling vacuum-packed meat products, such as ham. Since the temperature is the main factor affecting the microbial dynamic, the use of mathematical models describing the microbial behavior into a non-isothermal environment can be very useful for predicting food shelf life. In this study, the growth of Lactobacillus viridescens was measured in vacuum-packed sliced ham under non-isothermal conditions, and the predictive ability of primary (Baranyi and Roberts, 1994) and secondary (Square Root) models were assessed using parameters estimated in MRS culture medium under isothermal conditions (between 4 and 30°C). Fresh ham piece was sterilized, sliced, inoculated, vacuum-packed, and stored in a temperature-controlled incubator at five different non-isothermal conditions (between 4 and 25°C) and one isothermal condition (8°C). The mathematical models obtained in MRS medium were assessed by comparing predicted values with L. viridescens growth data in vacuum-packed ham. Its predictive ability was assessed through statistical indexes, with good results (bias factor between 0.95 and 1.03; accuracy factor between 1.04 and 1.07, and RMSE between 0.76 and 1.33), especially in increasing temperature, which predictions were safe. The model parameters obtained from isothermal growth data in MRS medium enabled to estimate the shelf life of a commercial ham under non-isothermal conditions in the temperature range analyzed.
Assuntos
Microbiologia de Alimentos/métodos , Embalagem de Alimentos/métodos , Conservação de Alimentos/métodos , Armazenamento de Alimentos , Lactobacillus/crescimento & desenvolvimento , Produtos da Carne/microbiologia , Modelos Biológicos , Animais , Contagem de Colônia Microbiana , Suínos , TemperaturaRESUMO
The aim of this study was to establish primary and secondary models to describe the growth kinetics of Byssochlamys fulva on solidified apple juice at different temperatures. B. fulva was inoculated on solidified apple juice at 10, 15, 20, 25 and 30 °C. Linear-with-breakpoint, Baranyi and Roberts, and Huang primary models (without upper asymptote) were fitted to the data, and they showed good ability to describe the growth kinetics. B. fulva showed longer adaptation time on apple juice than on culture medium, but growth rates were similar as reported in the literature. The dependence of µmax and λ parameters on temperature was described with Square Root and Arrhenius-Davey secondary models, respectively. These models were important to establish process/storage conditions and apple juice shelf life.
RESUMO
Dehydration and rehydration processes of Pleurotus ostreatus fruiting bodies were investigated in this work. Mushroom samples were dehydrated at 40, 50 and 60 ºC, using drying air with relative humidity of 75 percent. The rehydration was investigated at different temperatures of immersion water (25, 55 and 85 ºC) and different immersion times (30, 75 and 120 minutes). The best rehydration occurred for the samples dried at 40 ºC. The rehydration could be done in water at room temperature, during 30 minutes. Water sorption isotherms of samples were determined at 30, 40 and 50 ºC. Both GAB and BET models satisfactorily represented the experimental data of moisture sorption of dried mushrooms.
Processos de desidratação e de rehidratação de cogumelos da espécie Pleurotus ostreatus foram avaliados neste trabalho. Os cogumelos foram desidratados a 40, 50 e 60 ºC, com umidade relativa do ar de 75 por cento. O processo de rehidratação foi avaliado para diferentes temperaturas de água de imersão (25, 55 e 85 ºC) e diferentes tempos de imersão (30, 75 e 120 minutos). A melhor temperatura de secagem foi 40 ºC, levando em consideração a melhor rehidratação dos cogumelos desidratados nesta temperatura. A rehidratação pode ser feita em água a temperatura ambiente, por 30 minutos. Isotermas de sorção de umidade de amostras foram determinadas a 30, 40 e 50 ºC.Tanto o modelo de GAB quanto o de BET representaram satisfatoriamente os dados experimentais de isoterma de sorção de umidade.
RESUMO
Cassava starch, dolomitic limestone and eucalypt cellulose fibres were used to prepare foam trays that could be used to pack foodstuffs. The influence of the cellulose fibre concentration in the composite formulation was investigated using 5, 10, 15, 20, 30 and 40 percent of fibres. The results indicated that an increase in cellulose fibre concentration promoted a decrease in density and tensile strength of the foam samples. The tensile strength at break for foam trays containing 5 percent of cellulose fibres was 3.03MPa, whilst the commercial trays of expanded polystyrene used to pack foods in supermarkets presented a tensile strength of 1.49 MPa. The elongation at break of the foam trays obtained in this work varied slightly with increase in cellulose fibre concentration, the values being about 20 percent lower than the elongation at break observed for commercial foam trays of expanded polystyrene. Thus, the materials developed in this work represented a possible alternative to the use of EPS foam trays for packing dry foods. The trays' properties need to be improved for their use with moist foods.
Amido de mandioca, calcário dolomítico e fibras celulósicas de eucalipto foram usados para preparar bandejas biodegradáveis que podem ser usadas como embalagens para alimentos. A influência da concentração de fibras na formulação dos compostos foi investigada, usando 5, 10, 15, 20, 30 e 40 por cento de fibras. Os resultados indicaram que o aumento na concentração de fibras celulósicas promove uma diminuição na densidade e na resistência a tração das amostras. A tensão na ruptura para as bandejas contendo 5 por cento de fibras celulósicas foi de 3,03 MPa, enquanto as bandejas comerciais de poliestireno expandido (EPS) usadas para embalagens alimentícias em supermercados apresentaram uma tensão de 1.49 MPa. A elongação na ruptura das bandejas obtidas neste trabalho variou ligeiramente com o aumento da concentração de fibra celulósica, os valores são aproximadamente 20 por cento mais baixos do que a elongação na ruptura observada para bandejas comerciais de poliestireno expandido. Os resultados indicaram que as propriedades das bandejas devem ser melhoradas na utilização de embalagem de alimentos úmidos. Assim os materiais desenvolvidos neste trabalho representam uma possível alternativa para o uso de bandejas de EPS para embalar alimentos secos. As propriedades das bandejas necessitam ser melhoradas para sua utilização com alimentos úmidos.
RESUMO
The effect of sucrose solution temperature on the mechanical properties, water loss (WL), solids gain (SG) and weight reduction (WR) of apples (Fuji var.) treated by vacuum impregnation was studied. Temperatures were varied from 10 to 50 ºC, using a sucrose solution of 50 ºBrix. The mechanical properties were studied throughout a stress relaxation test. The results showed that the SG varied between 10.57 and 14.29 % and the WL varied between 10.55 and 14.48 %. The treated fruit soluble solids increased with the temperature probably due to the lower viscosity of the solution. The maximum stress was highest at 10 ºC, decreasing at higher temperatures, probably due the softening of the structure.
A impregnação a vácuo (VI) de alimentos é realizada pela aplicação de vácuo em um tanque contendo o produto imerso em uma solução, seguida da recuperação da pressão atmosférica. Neste trabalho, estudou-se o efeito da temperatura da solução de sacarose nas propriedades mecânicas das amostras e na perda de água (WL), ganho de sólidos (SG) e redução de peso (WR). A faixa de temperaturas estudada foi de 10 a 50 ºC, usando uma solução de sacarose com 50 ºBrix. As propriedades mecânicas das amostras foram estudadas através de ensaios mecânicos de deformação-relaxação. O SG variou entre 10.57 e 14.29 %, enquanto WL variou entre 10.55 e 14.48 %. O teor de sólidos das frutas tratadas aumentou com a temperatura, provavelmente devido à diminuição da viscosidade da solução. A tensão máxima foi maior a 10 ºC, diminuindo com a temperatura, devido ao amolecimento da estrutura.
RESUMO
The present work focuse on the impact of O2, CO2 and ethylene concentrations on ripening rate control of bananas as a contribution for the development of domestic equipments that could allow the user to drive the fruit shelf live. It represented the adjustment of metabolic activity rates in order to manage the maturity process. Ripening variables such as ethylene and CO2 concentrations and temperature were adjusted to accelerate or slow down the process, while the maturity degree was monitored through the physical and chemical parameters and sensorial analysis. Therefore, the objective of this work was to evaluate the influence of these parameters to manage the banana ripening. The optimum temperature was at 25 ºC of storage. The presence of oxygen, CO2 withdraws and ethylene injection were relevant for the ripening process. The "ready-to-eat" quality was achieved in 6 days in confined system. The use of ethylene as trigger was adequate to accelerate the ripening process with advantages in fruit color.
O presente trabalho foca no impacto da concentração de O2, CO2 e etileno no controle da taxa de amadurecimento de bananas, como contribuição para o desenvolvimento de equipamentos domésticos que permitam o controle pelo próprio usuário da vida de prateleira de frutas. Isto representa o ajuste das atividades metabólicas para garantir o controle do amadurecimento. Variáveis como concentração de etileno e CO2 e temperatura foram ajustadas para acelerar ou reduzir o processo, enquanto que o grau de maturação foi monitorado através de parâmetros físico-químicos e sensoriais. Desta forma, o objetivo deste trabalho foi avaliar a influência destes parâmetros para controlar o amadurecimento de banana. A temperatura ótima de amadurecimento foi 25ºC. A presença de O2, a retirada de CO2 e a injeção de etileno foram relevantes no processo. A qualidade "pronto-para-consumo" foi obtida em 6 dias em sistema confinado. O uso de etileno como gatilho é adequado para acelerar o amadurecimento, com vantagens para a cor do produto.