RESUMO
Sera from 102 inpatients from the Volgograd region (64) and Krasnodar region (38) were tested for antibodies to West Nile (WN) virus in hemagglutination inhibition (HI) test and for IgM and IgG antibodies in enzyme immunoassay (EIA). Diseases etiologically associated with WN virus were diagnosed in 81 patients: in 50 out of 64 in the Volgograd region and in 31 out of 38 in the Krasnodar region, which makes 79.4%. Specificity of antibodies to WN virus was confirmed in HI and EIA with WN antigens, related flaviviruses (Japanese encephalitis and yellow fever), and Sindbis alfavirus. A considerable number and the incidence of WN infection suggest that an epidemic caused by WN virus occurred in the Krasnodar and Volgograd regions in summer 1999.
Assuntos
Meningite Viral/epidemiologia , Meningoencefalite/epidemiologia , Vírus do Nilo Ocidental/patogenicidade , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Especificidade de Anticorpos , Surtos de Doenças , Humanos , Técnicas Imunoenzimáticas , Incidência , Meningite Viral/imunologia , Meningite Viral/virologia , Meningoencefalite/imunologia , Meningoencefalite/virologia , Federação Russa/epidemiologia , Vírus do Nilo Ocidental/imunologiaRESUMO
Two strains of West Nile virus LEIV 27889 Vig and Ast 986 were isolated from the brain of a dead subject and from the blood of a patient, respectively, during an outbreak of serous meningitis and meningoencephalitis in July-September, 1999, in the Volgograd region, Krasnodar territory, and Astrakhan region. These strains reacted with convalescent sera in hemagglutination inhibition test, which proves their etiological role in this outbreak.
Assuntos
Surtos de Doenças , Febre do Nilo Ocidental/epidemiologia , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/isolamento & purificação , Antígenos Virais/imunologia , Genoma Viral , Testes de Hemaglutinação , Humanos , Federação Russa/epidemiologia , Vírus do Nilo Ocidental/genética , Vírus do Nilo Ocidental/imunologiaRESUMO
Mosquito sampling was carried out in Volgograd city and its vicinity in August 2001 and 2002. In total 16,000 individuals belonging to 6 genera and 12 species were collected. Nine species were anthropophilic. Culex modestus and Aedes vexans dominated in all outdoor samples collected in Volgograd city. In addition to these species, Coquillettidia richiardii and Ae. caspius were abundant in the vicinity of Volgograd. Autogenous Cx. popiens dominated among six species sampled indoors in Volgograd city. The possible role of different mosquito species in West Nile virus circulation in Volgograd city is discussed.
Assuntos
Culicidae , Febre do Nilo Ocidental/prevenção & controle , Animais , Culicidae/genética , Culicidae/virologia , Surtos de Doenças , Ecossistema , Humanos , Fatores de Risco , Federação Russa/epidemiologia , Especificidade da Espécie , População Urbana , Febre do Nilo Ocidental/epidemiologiaRESUMO
From July to September 1999, a widespread outbreak of meningoencephalitis associated with West Nile virus (Flavivirus, Flaviviridae) occurred in southern Russia, with hundreds of cases and dozens of deaths. Two strains of West Nile virus isolated from patient serum and brain-tissue samples reacted in hemagglutination-inhibition and neutralization tests with patients' convalescent-phase sera and immune ascites fluid from other strains of West Nile virus.