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1.
Transl Psychiatry ; 7(2): e1034, 2017 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-28195573

RESUMO

Bipolar disorder affects about 1% of the world's population, and its estimated heritability is about 75%. Only few whole genome or whole-exome sequencing studies in bipolar disorder have been reported, and no rare coding variants have yet been robustly identified. The use of isolated populations might help finding variants with a recent origin, more likely to have drifted to higher frequency by chance. Following this approach, we investigated 28 bipolar cases and 214 controls from the Faroe Islands by whole exome sequencing, and the results were followed-up in a British sample of 2025 cases and 1358 controls. Seventeen variants in 16 genes in the single-variant analysis, and 3 genes in the gene-based statistics surpassed exome-wide significance in the discovery phase. The discovery findings were supported by enrichment analysis of common variants from genome-wide association studies (GWAS) data and interrogation of protein-protein interaction networks. The replication in the British sample confirmed the association with NOS1 (missense variant rs79487279) and NCL (gene-based test). A number of variants from the discovery set were not present in the replication sample, including a novel PITPNM2 missense variant, which is located in a highly significant schizophrenia GWAS locus. Likewise, PIK3C2A identified in the gene-based analysis is located in a combined bipolar and schizophrenia GWAS locus. Our results show support both for existing findings in the literature, as well as for new risk genes, and identify rare variants that might provide additional information on the underlying biology of bipolar disorder.


Assuntos
Transtorno Bipolar/genética , Óxido Nítrico Sintase Tipo I/genética , Fosfoproteínas/genética , Proteínas de Ligação a RNA/genética , Proteínas de Ligação ao Cálcio/genética , Estudos de Casos e Controles , Dinamarca , Redes Reguladoras de Genes , Predisposição Genética para Doença , Humanos , Proteínas de Membrana/genética , Proteínas de Membrana Transportadoras , Mutação de Sentido Incorreto , Fosfatidilinositol 3-Quinases/genética , Polimorfismo Genético , Análise de Sequência de DNA , Reino Unido , Nucleolina
2.
Free Radic Res ; 40(12): 1303-23, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17090420

RESUMO

Many epidemiological data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and allelic variants of genes have been described. A breakthrough strategy to get insight into the genetics of longevity is the study of centenarians, the best example of successful ageing. We review the main results regarding nuclear genes as well as the mitochondrial genome, focusing on the investigations performed on Italian centenarians, compared to those from other countries. These studies produced interesting results on many putative "longevity genes". Nevertheless, many discrepancies are reported, likely due to the population-specific interactions between gene pools and environment. New approaches, including large-scale studies using high-throughput techniques, are urgently needed to overcome the limits of traditional association studies performed on a limited number of polymorphisms in order to make substantial progress to disentangle the genetics of a trait as complex as human longevity.


Assuntos
Envelhecimento/genética , Genes , Longevidade/genética , Idoso de 80 Anos ou mais , Animais , Apolipoproteína E4/genética , Apolipoproteínas/genética , Arildialquilfosfatase/genética , Clusterina/genética , Citocinas/genética , DNA Mitocondrial/genética , Humanos , Inflamação/genética , Fator de Crescimento Insulin-Like I/genética , Poli(ADP-Ribose) Polimerases/genética , Polimorfismo Genético , Complexo de Endopeptidases do Proteassoma/fisiologia , Superóxido Dismutase/genética , Proteína Supressora de Tumor p53/genética
3.
Leukemia ; 26(5): 902-9, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22076464

RESUMO

Acute lymphoblastic leukemia (ALL) is a malignant disease of the white blood cells. The etiology of ALL is believed to be multifactorial and likely to involve an interplay of environmental and genetic variables. We performed a genome-wide association study of 355 750 single-nucleotide polymorphisms (SNPs) in 474 controls and 419 childhood ALL cases characterized by a t(12;21)(p13;q22) - the most common chromosomal translocation observed in childhood ALL - which leads to an ETV6-RUNX1 gene fusion. The eight most strongly associated SNPs were followed-up in 951 ETV6-RUNX1-positive cases and 3061 controls from Germany/Austria and Italy, respectively. We identified a novel, genome-wide significant risk locus at 3q28 (TP63, rs17505102, P(CMH)=8.94 × 10(-9), OR=0.65). The separate analysis of the combined German/Austrian sample only, revealed additional genome-wide significant associations at 11q11 (OR8U8, rs1945213, P=9.14 × 10(-11), OR=0.69) and 8p21.3 (near INTS10, rs920590, P=6.12 × 10(-9), OR=1.36). These associations and another association at 11p11.2 (PTPRJ, rs3942852, P=4.95 × 10(-7), OR=0.72) remained significant in the German/Austrian replication panel after correction for multiple testing. Our findings demonstrate that germline genetic variation can specifically contribute to the risk of ETV6-RUNX1-positive childhood ALL. The identification of TP63 and PTPRJ as susceptibility genes emphasize the role of the TP53 gene family and the importance of proteins regulating cellular processes in connection with tumorigenesis.


Assuntos
Subunidade alfa 2 de Fator de Ligação ao Core/genética , Predisposição Genética para Doença , Mutação em Linhagem Germinativa , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Proteínas Proto-Oncogênicas c-ets/genética , Proteínas Repressoras/genética , Estudos de Casos e Controles , Criança , Cromossomos Humanos Par 3 , Estudo de Associação Genômica Ampla , Humanos , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Variante 6 da Proteína do Fator de Translocação ETS
4.
Curr Pharm Des ; 16(7): 802-13, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20388091

RESUMO

Human aging and longevity are complex and multi-factorial traits that result from a combination of environmental, genetic, epigenetic and stochastic factors, each contributing to the overall phenotype. The multi-factorial process of aging acts at different levels of complexity, from molecule to cell, from organ to organ systems and finally to organism, giving rise to the dynamic "aging mosaic". At present, an increasing amount of experimental data on genetics, genomics, proteomics and other -omics are available thanks to new high-throughput technologies but a comprehensive model for the study of human aging and longevity is still lacking. Systems biology represents a strategy to integrate and quantify the existing knowledge from different sources into predictive models, to be later tested and then implemented with new experimental data for validation and refinement in a recursive process. The ultimate goal is to compact the new acquired knowledge into a single picture, ideally able to characterize the phenotype at systemic/organism level. In this review we will briefly discuss the aging phenotype in a systems biology perspective, showing four specific examples at different levels of complexity, from a systemic process (inflammation) to a cascade-process pathways (coagulation) and from cellular organelle (proteasome) to single gene-network (PON-1), which could also represent targets for anti-aging strategies.


Assuntos
Envelhecimento/fisiologia , Longevidade/fisiologia , Biologia de Sistemas , Fatores Etários , Desenho de Fármacos , Humanos , Modelos Biológicos
5.
Expert Opin Biol Ther ; 8(9): 1393-405, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18694357

RESUMO

BACKGROUND: The aging phenotype in humans is very heterogeneous and can be described as a complex mosaic resulting from the interaction of a variety of environmental, stochastic and genetic-epigenetic variables. Therefore, each old person must be considered as a singleton, and consequently the definition of 'aging phenotype' is very difficult. OBJECTIVE: We discuss the phenotype of centenarians, the best example of successful aging, as well as other models exploited to study human aging and longevity, such as families enriched in long-living subjects, twins and cohorts of unrelated subjects. METHODS: A critical review of literature available until March 2008. CONCLUSIONS: No single model can be considered the gold standard for the study of aging and longevity, instead the combination of results obtained from different models must be considered in order to better understand these complex phenomena. We propose that a systems biology concept such as that of 'bow-tie' architecture, useful for managing information flow, could help in this demanding task.


Assuntos
Envelhecimento , Longevidade , Modelos Biológicos , Idoso , Idoso de 80 Anos ou mais , Humanos , Fenótipo
6.
Aging (Milano) ; 13(3): 163-70, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11442299

RESUMO

The role of interleukin 1 (IL-1) and interleukin 6 (IL-6) in the pathogenesis of Alzheimer's disease (AD) is reviewed within the framework of "inflamm-aging", i.e., the characteristic chronic pro-inflammatory status which develops in old age, and neuroinflammation, i.e., the peculiar inflammatory process which is present in the brain of AD patients. In particular, the data suggesting that several IL-1 and IL-6 gene polymorphisms can contribute to the risk of developing AD are reviewed. The possibility as well as the difficulty in identifying a pro-inflammatory phenotype, and its importance for the prevention, diagnosis and therapy of AD and other age-related pathologies are discussed.


Assuntos
Doença de Alzheimer/genética , Idoso , Envelhecimento/genética , Envelhecimento/imunologia , Doença de Alzheimer/etiologia , Doença de Alzheimer/imunologia , Citocinas/biossíntese , Humanos , Mediadores da Inflamação/metabolismo , Interleucina-1/biossíntese , Interleucina-1/genética , Interleucina-6/biossíntese , Interleucina-6/genética , Pessoa de Meia-Idade , Neurite (Inflamação)/genética , Fenótipo
7.
Aging (Milano) ; 12(2): 77-84, 2000 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10902049

RESUMO

Gender accounts for important differences in the incidence and prevalence of a variety of age-related diseases. Considering people of far advanced age, demographic data document a clear-cut prevalence of females compared to males, suggesting that sex-specific mortality rates follow different trajectories during aging. In the present investigation, we report data from a nationwide study on Italian centenarians (a total of 1162 subjects), and from two studies on centenarians living in two distinct zones of Italy, i.e., the island of Sardinia (a total of 222 subjects) and the Mantova province (Northern Italy) (a total of 43 subjects). The female/male ratio was about 2:1 in Sardinia, 4:1 in the whole of Italy, and about 7:1 in the Mantova province. Thus, a complex interaction of environmental, historical and genetic factors, differently characterizing the various parts of Italy, likely plays an important role in determining the gender-specific probability of achieving longevity. Gender differences in the health status of centenarians are also reported, and an innovative score method to classify long-lived people in different health categories, according to clinical and functional parameters, is proposed. Our data indicate that not only is this selected group of people, as a whole, highly heterogeneous, but also that a marked gender difference exists, since male centenarians are less heterogeneous and more healthy than female centenarians. Immunological factors regarding the age-related increase in pro-inflammatory status, and the frequency of HLA ancestral haplotypes also show gender differences that likely contribute to the different strategies that men and women seem to follow to achieve longevity. Concerning the different impact of genetic factors on the probability of reaching the extreme limits of the human life-span, emerging evidence (regarding mtDNA haplogroups, Thyrosine Hydroxilase, and IL-6 genes) suggests that female longevity is less dependent on genetics than male longevity, and that female centenarians likely exploited a healthier life-style and more favorable environmental conditions, owing to gender-specific cultural and anthropological characteristics of the Italian society in the last 100 years.


Assuntos
Longevidade , Caracteres Sexuais , Idoso , Idoso de 80 Anos ou mais , Feminino , Nível de Saúde , Humanos , Sistema Imunitário/fisiologia , Longevidade/genética , Masculino , Estresse Fisiológico/fisiopatologia
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