Strigolactone analog GR24 triggers changes in PIN2 polarity, vesicle trafficking and actin filament architecture.

Strigolactones (SLs) are plant hormones that regulate shoot and root development in a MAX2-dependent manner. The mechanism underlying SLs' effects on roots is unclear. We used root hair elongation to measure root response to SLs. We examined the effects of GR24 (a synthetic, biologically active SL analog) on localization of the auxin efflux transporter PIN2, endosomal trafficking, and F-actin architecture and dynamics in the plasma membrane (PM) of epidermal cells of the primary root elongation zone in wildtype (WT) Arabidopsis and the SL-insensitive mutant max2. We also recorded the response to GR24 of trafficking (tir3), actin (der1) and PIN2 (eir1) mutants. GR24 increased polar localization of PIN2 in the PM of epidermal cells and accumulation of PIN2-containing brefeldin A (BFA) bodies, increased ARA7-labeled endosomal trafficking, reduced F-actin bundling and enhanced actin dynamics, all in a MAX2-dependent manner. Most of the der1 and tir3 mutant lines also displayed reduced sensitivity to GR24 with respect to root hair elongation. We suggest that SLs increase PIN2 polar localization, PIN2 endocytosis, endosomal trafficking, actin debundling and actin dynamics in a MAX2-dependent fashion. This enhancement might underlie the WT root's response to SLs, and suggests noncell autonomous activity of SLs in roots.

Assessment of a limited-access parotidectomy technique's complications and scar characteristics - A cohort study.

OBJECTIVE: Parotidectomy is the treatment of choice for benign tumors of the parotid gland, with the modified Blair incision most commonly utilized. This retrospective analysis aimed to determine the incidence of complications and assess the relationship between the mass and scar characteristics, in patients who had undergone parotidectomy. MATERIALS AND METHODS: The scar characteristics of patients who had undergone parotidectomy for benign neoplasms at our medical center between 2013 and 2019 were evaluated. RESULTS: Overall, 49 patients met the inclusion criteria, of whom 33 agreed to participate in the study. The mean patient age was 52.8 years; 57.5% were males. No correlation was identified between the parotid mass size or location and scar type or length. The most common complications in this study were similar to those reported in the literature. CONCLUSION: The results of this study suggest that the smaller, tailored modified Blair incision for parotidectomy does not increase complication rates. Future studies with larger cohorts should be conducted to further assess the potential benefits of tailored incision size during modified Blair parotidectomies.