An improved method of local coloring in vertical-slot fishways to enhance the fish migration effect of grass carp.

Vertical-slot fishway (VSF) has been used in many water conservancy projects to restore the river connectivity. A high-quality fishway project should facilitate fish to discovering the exit and passing through, avoiding to long stay in the fishway and delay the migration. Current research on fishway engineering has not yielded an expected passing ratio of fish migration, and it is therefore of great significance to further study the assisting effect of VSF in fish migration. To begin with, we preliminarily determined the attractive and repelling colors of grass carps based on their swimming behavior in a static water pool configured with local colors. Combined with the migration route of the grass carp in a VSF pool without local coloring, four local coloring cases were designed. Based on the camera results of the four experimental local coloring cases, a comparative analysis was conducted with the blank control group frame by frame. This was followed by the statistics of the number of successfully migrated grass carps and their total completion time. On that basis, the assisting effect of VSF in fish migration under the four cases was evaluated in terms of the reduction rate of migration route length, the reduction rate of completion time, and the improvement rate of passing ratio. The research outcomes indicated that green and blue act as attractive colors while yellow and red serve as repelling colors for grass carp. Adding colors to the training wall and dividing wall in the VSF pool, the migration route of grass carp was appropriately adjusted, alongside a shortened completion time and an improved passing ratio. Of the four local coloring cases, the recommended case showed a significant effect on migration route, with more concentrated moving trajectories and shortened route length. Typically, the migration route length decreased by 26%, and the frequency of fish long staying at the junction between the training wall and dividing wall was markedly reduced, as well as the frequency of fish swimming along the water flow from upstream to downstream. The completion time was shortened by 26%, and the passing ratio was enhanced by 44%. The approach of combining local coloring with fish behavior and fishway hydraulics in the pool surpassed the method that optimizes the fishway design only from the fishway hydraulics. The improved method greatly shortened the migration route length, reduced the completion time, and significantly improved the passing ratio of fish passage objects in the VSF. The present research mainly focuses on using model experiments to evaluate the local coloring cases. In the future studies, we will configure local colors to the sidewalls of on-site fishways using environmentally friendly paint or colored organic glass panels. With the monitoring results of the completion time and passing ratio of fish passage objects, the recommended case can be further verified and optimized, thereby providing a more reasonable and feasible local coloring case for assisting fish migration in the VSF project.

Safety and efficacy of glibenclamide combined with rtPA in acute cerebral ischemia with occlusion/stenosis of anterior circulation (SE-GRACE): study protocol for a randomized controlled trial.

BACKGROUND: Thrombolysis with recombinant tissue plasminogen activator (rtPA) improves outcome for patients with acute ischemic stroke (AIS), but many of them still have substantial disability. Glibenclamide (US adopted name, glyburide), a long-acting sulfonylurea, shows promising result in treating AIS from both preclinical and clinical studies. This study investigates the safety and efficacy of glibenclamide combined with rtPA in treating AIS patients. METHODS: This is a prospective, randomized, double-blind, placebo-controlled, multicenter trial with an estimated sample size of 306 cases, starting in January 2018. Patients aged 18 to 74 years, presented with a symptomatic anterior circulation occlusion with a deficit on the NIHSS of 4 to 25 points and treated with intravenous rtPA within the first 4.5 h of their clinical onsets, are eligible for participation in this study. The target time from the onset of symptoms to receive the study drug is of 10 h. Subjects are randomized 1: 1 to receive glibenclamide or placebo with a loading dose of 1.25 mg, followed by 0.625 mg every 8 h for total 5 days. The primary efficacy endpoint is 90-day good outcome, measured as modified Rankin Scale of 0 to 2. Safety outcomes are all-cause 30-day mortality and early neurological deterioration, with a focus on cardiac- and glucose-related serious adverse events. DISCUSSION: This study will provide valuable information about the safety and efficacy of oral glibenclamide for AIS patients treated with rtPA. This would bring benefits to a large number of patients if the agent is proved to be effective. TRIAL REGISTRATION: The trial was registered on September 14th 2017 at www.clinicaltrials.gov having identifier NCT03284463. Registration was performed before recruitment was initiated.

The effect of nonpharmacologic therapy on global cognitive functions in patients with Alzheimer's disease: an updated meta-analysis of randomized controlled trials.

Background: The effectiveness of non-pharmacologic therapy (NPT) in treating the global cognition dysfunction associated with Alzheimer's disease (AD) has not been clearly demonstrated. Therefore, we performed a meta-analysis to address this issue.Methods: The Cochrane Central Register of Controlled Trials, PUBMED, EMBASE and other databases were searched, and outcomes measured by the Mini-Mental State Examination (MMSE) or the Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-cog) were analysed.Results: Seven types of NPT were included, 25 randomized controlled trials (RCTs) were selected and 3238 participants were included in the meta-analysis. There were significant differences between the NPT and control groups in the MMSE and ADAS-cog scores.Conclusions: Although more extensive trials need to be performed, NPT has been observed to be beneficial in AD patients.

Effect of flow rate of side-type orifice intake on withdrawn water temperature.

Side-type orifice intake is a type of selective withdrawal facility used in managing reservoirs to mitigate the negative effects of low-temperature water. Based on the temperature data of a thermal stratified reservoir in China, an experiment was conducted in flume to study the influence of intake flow rate on withdrawn water temperature with different temperature distributions. Results indicated that withdrawn water temperature changed with different flow rates. The temperature change was determined by the water temperature gradients above and below the intake, whereas the change trend of temperature depended on the difference between the water temperature gradient above and below the intake. We likewise proposed a new equation with which the withdrawn water temperature of a thermal stratified reservoir using a side-type orifice could be calculated. These findings could be directly applied to the design and operation of side-type orifice intake in thermal stratified reservoirs.

Occurrence, distribution, and ecological risk assessment of artificial sweeteners in surface and ground waters of the middle and lower reaches of the Yellow River (Henan section, China).

As a new class of water contaminants, artificial sweeteners (ASs) have attracted much attention due to their environmental persistence and potential adverse effects to human and the environment. This study systematically investigated the occurrence and distribution of four commonly used ASs in the effluent of wastewater treatment plants (WWTPs), surface water and groundwater in the middle and lower reaches of the Yellow River (Henan section). Sucralose (SUC) was dominant in WWTP effluents and had the highest mass loading. Acesulfame (ACE), cyclamate (CYC), saccharin (SAC), and SUC were consistently detected in surface water at concentrations ranging from 1.364 ng/L (CYC) to 7786 ng/L (ACE). Spatial analysis showed that the pollution level of ASs in the trunk stream was lower than that in most tributaries. The total concentrations of ASs detected in surface water ranged between 308.7 and 10,498 ng/L, while in groundwater, the total concentration of ASs detected was between ND-4863 ng/L. ACE and SUC are the main pollutants in surface water and groundwater within this survey area. The risk assessment showed that the risks of the four target ASs to aquatic organisms were negligible (risk quotient (RQ) values < 0.1), and the maximum risk quotient of the mixtures (MRQ) values of all rivers were all much less than 0.1.

Determination of tryptophan and its indole metabolites in follicular fluid of women with diminished ovarian reserve.

Tryptophan (TRP) and its indole metabolites exhibit numerous biological effects, especially their antioxidant properties. This study used untargeted metabolomics in conjunction with targeted metabolomics to investigate the differential expression of tryptophan and its indole metabolites in follicular fluid (FF) of diminished ovarian reserve (DOR) and normal ovarian reserve (NOR) populations. This study included patients with DOR (n = 50) and females with NOR (n = 35) who received in vitro fertilization and embryo transfer. Untargeted metabolomics suggests that diminished ovarian reserve affects the metabolic profile of FF, TRP and indole metabolites were significantly down-regulated in the DOR group. Targeted metabolomics quantification revealed that the levels of TRP, IPA and IAA in the FF of the DOR group were significantly lower than those of the NOR group (P < 0.01). The concentration of TRP in FF is positively correlated with the available embryo rate in NOR females. These results provide data support to explore the pathogenesis of DOR and to look for new biomarkers and ovarian protectors. Additionally, alterations in TRP and its indole metabolites in FF may indirectly reflect the interaction between intestinal flora and the follicular microenvironment.

Experimental study on the passage behavior of juvenile Schizothorax prenanti by configuring local colors in the vertical slot fishways.

The vertical slot fishway is one of the most widely used fishways in the world. To solve the problems of long passage time and low passage rate of fish passing objects in the conventional vertical slot fishway, this study proposed a method of configuring local colors in the internal sidewall of the fishway pond to improve the vertical slot fishway based on the approach-avoidance effect of fishes on different colors. Taking Schizothorax prenanti as the research object, comparative fish passing experiments were carried out to analyze the effects of configuring local colors on the passage behavior. It has been found that green and yellow are the approach and repellent colors for Schizothorax prenanti, respectively. By configuring local colors of the guide wall and partition wall at the upstream and downstream of the pond, the passage track of Schizothorax prenanti was significantly changed, which in turn affected their passage time and passage rate. The configuration of local green to the guide wall and partition wall on the upstream negative side, and local yellow to the sidewall of the guide wall on the upstream negative side, the guide wall on the downstream positive side, and the partition wall on the downstream positive side can shorten the overall passage time of Schizothorax prenanti by 9 % and increase the passage rate by 23 %. Compared with the scheme that completely depends on the flow conditions to change the structure of the pond, the method of configuring local colors in the pond is an improved method combining fish ethology and hydraulics. The scheme of local color configuration significantly shortens the passage time and passage rate of fish passing objects in the vertical slot fishway, and can be applied to the fishway design and the improvement of operation effect, which has a certain practical application value.

A Novel Strategy for Unveiling Spatial Distribution Pattern of Gallotannins in Paeonia rockii and Paeonia ostii Based on LC-QTRAP-MS.

Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC-MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops Paeonia rockii and P. ostii. According to the fragmentation behavior of the representative GT (1,2,3,4,6-penta-O-galloyl-ß-D-glucose, PGG), the diagnostic neutral loss (NL) of 170 Da was chosen for the non-targeted screening of GT precursors. Simultaneously, the tandem mass spectrometry spectrum (MS/MS) information was acquired through an enhanced product ion (EPI) scan. Nine major GTs were identified in tree peony. To quantify the targeted GTs in different tissues of tree peony, we established a multiple reaction monitoring (MRM)-enhanced product ion (EPI)-based pseudo-targeted approach under the information-dependent acquisition (IDA) mode. The quantitative results show that the GT compounds were ubiquitous in tree peony plants with diverse structures. The typical GT PGG was mainly distributed in roots, leaves, and petals. This strategy can also be utilized for metabolite characterization and quantification in other substrates.

Schizothorax prenanti swimming behavior in response to different flow patterns in vertical slot fishways with different slot positions.

Fish swimming behavior is a critical parameter for the design of vertical slot fishways. Although Schizothorax prenanti is a significant reproductive potamodromous migratory fish species in the upper reaches of the rivers in Southwest China, its swimming behavior has not been extensively researched. Therefore, in this study, a comparative experiment was conducted in vertical slot fishways to analyze the behavior of Schizothorax prenanti in response to different flow patterns, with respect to different slot positions. The experimental model was designed with a length scale of 1:4, and a method for selecting the appropriate fish size in the scaled physical model was proposed. Based on these experiments, it was found that the typical upstream trajectories of Schizothorax prenanti are traceable to the sidewall of the pool, which are characterized by low velocity and turbulent kinetic energy (TKE). The hydraulic variables exhibited an asymmetric distribution within the vertical slot, and the fish were found to pass through the area with the lowest velocity and TKE. A flow pattern with a guide wall length-to-pool width ratio of P/B = 0.25, in which Schizothorax prenanti can immediately find the sidewall, is suitable for fish migration. Therefore, this is the recommended value for the construction of effective vertical-slot fishway structures.

Emodin in atherosclerosis prevention: Pharmacological actions and therapeutic potential.

Atherosclerotic plaque formation, destabilization and eventual rupture leads to the acute cardiovascular events including myocardial infarction and stroke. Emodin (PubChem CID#3220), (1,3,8-trihydroxy-6-methylanthracene-9,10-dione) is a pharmacologically bioactive constituent isolated from the traditional Chinese medicinal herb Radix rhizoma Rhei. This molecule has anti-oxidant, anti-inflammatory, anti-proliferative, anti-apoptotic and lipid-modulating effects. Experimental studies have demonstrated that emodin attenuates and stabilizes atherosclerotic plaques. In this mini-review, we provide a summary of the pharmacological actions of emodin in regulating vascular function and atherosclerosis, highlighting the therapeutic potential of this phytochemical in patients with cardiovascular disease.