RESUMO
Background: Early recurrence (ER) after radical resection of hepatocellular carcinoma (HCC) affects the prognosis of patients. Gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) can improve the detection rate of small HCC. This study innovatively introduces a new quantitative index combined with qualitative index to compare the differences in clinical and imaging characteristics between ER and non-ER groups and evaluate the feasibility of Gd-EOB-DTPA-enhanced MRI in predicting ER. Methods: A total of 68 patients with HCC confirmed by operation and pathology in the Shandong Cancer Hospital and Institute were included retrospectively. All participants were examined by Gd-EOB-DTPA-enhanced MRI within 3 weeks before surgery. Regular follow-up was performed every 2 months within 1 year after operation. Among them, 18 cases with new lesions were in ER group, and 50 cases without new lesions were in non-ER group. The clinical and imaging data of the 2 groups were collected, and the differences of clinical data and preoperative MRI signs between the ER group and non-ER group were compared. The predictive factors of ER after HCC were analyzed by multivariate logistic regression. Results: The quantitative parameter lesion-to-liver contrast enhancement ratio (LLCER) can predict the pathological grade of HCC (P=0.023). The results of univariate analysis between the ER group and non-ER group showed that there were significant differences in pathological grade (P=0.008), lesion morphology (P=0.011), peritumoral low signal intensity in hepatobiliary phase (HBP) (P<0.001), satellite nodules (P<0.001), and LLCER (P<0.001) between the 2 groups. Multivariate logistic regression analysis showed that HBP peritumoral low signal intensity [odds ratio (OR) =7.214, 95% confidence interval (CI): 1.230-42.312, P=0.029], satellite nodules (OR =9.198, 95% CI: 1.402-60.339, P=0.021), and parameter LLCER value (OR =0.906, 95% CI: 0.826-0.995, P=0.039) were independent predictors of ER of HCC after resection. Conclusions: Preoperative Gd-EOB-DTPA enhanced MRI has important predictive value for early recurrence after radical resection of hepatocellular carcinoma.
RESUMO
Ovarian cancer is one of the most common malignancies in women and characterized by a rapid progression to metastasis, which restricts effective treatment options. Caffeic acid phenethyl ester (CAPE), a natural honeybee product, exhibits a variety of biological activities, anti-tumor is included. Our study aims to explore whether CAPE could inhibit the progression of ovarian cancer and the underlying molecular mechanism. The establishment of ovarian cancer model was set up in mice through caudal vein injection of SKOV-3 cells. Results indicated that CAPE treatment remarkably decreased the viability, migration and invasion of SKOV-3 cells. Besides that, the apoptosis of SKOV-3 cells was significantly promoted by CAPE treatment. Moreover, the growth of ovarian cancer was tremendously inhibited by CAPE in vivo and its action was accompanied by the obstructed Ki67 and PCNA expression. Furthermore, nuclear factor kappa b (NF-?B) pathway was dramatically suppressed by CAPE through the inhibition of I?B phosphorylation, nuclear translocation of p65 and NF-?B p65 DNA binding activity. By contrast, cells transfected with p65 siRNA exhibited decreased cell viability, migration and invasion along with increased cell apoptosis in SKOV-3 cells. However, CAPE treatment could enhance these alters induced by p65 siRNA in KOV-3 cells. Taken together, these findings suggested that CAPE could restrain the progression of ovarian cancer via inactivating NF-?B signaling, and may provide novel therapeutic regimens for ovarian cancer.