Impacts of continuous quality improvement on wound pain in the puncture site of arteriovenous fistula in haemodialysis patient.

This study analyses the effects of a continuous quality improvement nursing model on wound pain at the arteriovenous fistula (AVF) puncture site in patients undergoing haemodialysis. Forty haemodialysis patients from the First Affiliated Hospital of Chongqing Medical University, from September 2020 to December 2022, were selected as study subjects. They were randomly divided into an observation group and a control group. The control group received conventional nursing care, while the observation group was treated with a continuous quality improvement nursing model. The study compared the impact of these nursing approaches on pain intensity post-AVF puncture, wound visual analogue scale scores, self-rating anxiety scale, self-rating depression scale, quality of life scores and patient satisfaction with nursing care. In the observation group, the proportion of patients experiencing moderate to severe pain during AVF puncture was lower than that in the control group, whereas the proportion of patients with no pain or mild pain was higher (P = 0.008). After nursing, the observation group exhibited significantly lower wound visual analogue scale scores, self-rating anxiety scale scores, and self-rating depression scale scores compared to the control group (P < 0.001), with a significantly higher quality of life score (P < 0.05). The nursing satisfaction rate was 95.00% in the observation group, significantly higher than the 65.00% in the control group (P = 0.018). The continuous quality improvement nursing model significantly reduces wound pain at the AVF puncture site in haemodialysis patients, alleviates negative emotions, enhances the quality of life, and achieves high patient satisfaction. It is thus a highly recommendable approach in nursing practice.

Insights into Nitrogen-doped Carbon for Oxygen Reduction: The Role of Graphitic and Pyridinic Nitrogen Species.

Nitrogen-doped carbons (N/Cs) manifest good catalytic performance for oxygen reduction reaction (ORR) for fuel cell systems. However, to date, controversies remain on the role of active sites in N/Cs. In the present study, ORR test was conducted on three N/Cs in O2 -saturated 0.1 M KOH aqueous solution, where apparent linear correlation between graphitic N contents and ORR activity was observed. Theoretical calculations demonstrated that graphitic N doping is energetically more favorable than that of pyridinic N doping for ORR and the pyridinic N leads to more preferential with 2 e- ORR pathway. These results reveal that graphitic N plays a key role in N/Cs mediated ORR activity. This work lays a solid foundation on identifying the active sites in heteroatom-doped carbons and can be exploited for rational design and engineering of effective carbon-based ORR catalysts.

Solid-like Slippery Surface with Excellent Comprehensive Performance.

Slippery surfaces with outstanding slippery performances have shown application prospects in various fields, including anti-icing, antifouling, droplet transportation, and fog collection. However, practical application of the existing slippery surfaces is limited by lubricating oil loss, low water-slippery ability, low surface robustness, complex processes, and high costs. To overcome these limitations, we propose a facile, low-cost method to create a solid-like slippery Al surface (SSS-Al) by mixing hydrophobic nano-ceramic coating, silicone oil, and nano-SiO2, which shows excellent comprehensive performance. The SSS-Al shows exceptional water-slippery ability with a sliding angle of 5° and antifouling ability. Durability and chemical stability tests confirm the high surface durability and chemical stability of SSS-Al. Furthermore, SSS-Al exhibits anti-icing performance, fog collection ability, and electrochemical corrosion resistance, as well as demonstrates remarkable application prospects in important fields such as aerospace and shipbuilding.

γ-Glutamylcysteine rescues mice from TNBS-driven inflammatory bowel disease through regulating macrophages polarization.

OBJECTIVE: To explore the molecular mechanism of γ-glutamylcysteine (γ-GC) in response to inflammation in vivo and in vitro on regulating the polarization of macrophages. METHODS: The expressions of gene or protein were assessed by qPCR and Western blot assays, respectively. Cell viability was investigated by CCK-8 assay. Eight-week-old male BALB/c mice were established to examine the therapeutic effects of γ-GC in vivo. The release of TNF-α and IL-4 was determined by ELISA assay. Macrophages polarization was identified by flow cytometry assay. RESULTS: Our data showed that γ-GC treatment significantly improved the survival, weight loss, and colon tissue damage of IBD mice. Furthermore, we established M1- and M2-polarized macrophages, respectively, and our findings provided evidence that γ-GC switched M1/M2-polarized macrophages through activating AMPK/SIRT1 axis and inhibiting inflammation-related signaling pathway. CONCLUSION: Collectively, both in vivo and in vitro experiments suggested that γ-GC has the potential to become a promising novel therapeutic dipeptide for the treatment of IBD, which provide new ideas for the treatment of inflammatory diseases in the future.

Rheological behavior of xanthan gum suspensions with Fe-based nanoparticles: the effect of nanoparticles and the mechanism.

The rheological behavior of a xanthan gum (XG) suspension with Fe-based nanoparticles (Fe-NPs), e.g., nanoparticles of zerovalent iron (nZVI) and Fe3O4 (nFe3O4), needs to be understood for better injection of Fe-NPs for groundwater remediation. In this study, the rheological behavior of a XG suspension of nZVI and nFe3O4 was investigated at different particle concentrations. The Ostwald, Sisko, Williamson, and Cross models were employed to fit the rheological behavior of the suspensions for quantitatively describing the effect of the particles. The results showed that the viscosity of the XG solutions decreased with increasing particle concentrations and they maintained shear thinning properties. The Cross model was the best among the four models to describe the shear thinning behavior of the XG solution in the presence of the particles. According to Cross model analysis, increasing particle concentrations increased the degree of shear thinning behavior, as indicated by the increase of the power index (n). Also, the relaxation time (λ) decreased with increasing particle concentrations, which indicated an increase of molecule movement of XG. Compared with nFe3O4, nZVI resulted in a larger decrease in viscosity and a larger increase in the degree of shear thinning behavior. There was a good linear relation between n and λ for the suspensions (R2 = 0.85), which indicated that increasing molecule movement of XG was an important mechanism for the particles to intensify the shear thinning rheological behavior of the XG suspension of Fe-NPs. This study added insight into the knowledge of the rheological properties of the XG suspension of Fe-NPs, which is of importance for the field injection effort.

Pollution Characteristics and Associated Risk Assessment of Heavy Metals in Farmland Soils From a Typical County of Hubei Province, Central China.

Knowledge from the negative impacts of the counties' anthropogenic activities on soil pollution was of great significance in China, and valuable information was urgently needed for the control and remediation of soil pollution. The current pollution levels of heavy metals (Cu, Pb, Cd, Zn, Ni, and Cr) in farmland soils were investigated in Yangxin County, Hubei Province, central China. The comprehensive results of quantitative comparison and evaluation in this study showed that Cu (144.9 ± 298.6 mg kg-1), Cd (2.9 ± 1.6 mg kg-1), and Ni (137.0 ± 111.0 mg kg-1) posed higher pollution risks to public and ecosystem health, which were higher than the corresponding soil background values. The combined results of geostatistics, spatial and statistical analysis indicated that studied heavy metals were mainly attributed to agricultural, traffic and industrial induced pollution. Overall, urgent attention should be paid to the risk reduction and management of soil Cu, Cd, and Ni pollution in the study area.

Cardamonin, a natural chalcone, reduces 5-fluorouracil resistance of gastric cancer cells through targeting Wnt/ß-catenin signal pathway.

Objectives Cardamonin (CD), an active chalconoid, has been extensively studied in a wide variety of human tumors. However, the effects and underlying mechanism of cardamonin on 5-fluorouracil (5-FU)-resistant gastric cancer (GC) remain largely unclear. This study aimed to investigate the antitumor effects of cardamonin on 5-FU-resistant GC cells and explore the molecular mechanisms underlying its therapeutic potential. Methods The antitumor activities of cardamonin, 5-FU and their combination against BGC-823 and BGC-823/5-FU cells were determined using cytotoxicity assay, flow cytometry-based cell cycle analysis and Annexin V apoptosis assay. The effect of cardamonin on P-glycoprotein activity was assessed by Rh123 uptake assay. Real-time PCR, Western blotting and Co-immunoprecipitation analysis were carried out to assess the inhibition of Wnt/ß-catenin signaling pathway. A xenograft mouse model was established using BALB/c nude mice to examine the combinatorial effects of cardamonin and 5-FU on tumor growth. Results Our data provided the first demonstration that cardamonin significantly enhanced the chemosensitivity of 5-FU in GC cells via suppression of Wnt/ß-catenin signaling pathway. Additionally, the combination of cardamonin and 5-FU might result in the apoptosis and cell cycle arrest of BGC-823/5-FU cells, accompanied by the downregulated expression levels of P-glycoprotein, ß-catenin and TCF4. More importantly, our results demonstrated that cardamonin specifically disrupted the formation of ß-catenin/TCF4 complex, leading to TCF4-mediated transcriptional activation in 5-FU-resistant GC cells. Besides, through a xenograft mouse model, co-administration of cardamonin and 5-FU significantly retarded tumor growth in vivo, thus, confirming our in vitro findings. Conclusions Overall, this study revealed that cotreatment of cardamonin and 5-FU could strongly potentiate the antitumor activity of 5-FU, and put forth cardamonin as a rational therapeutic strategy for drug-resistant GC treatment.

Polycyclic aromatic hydrocarbons in railway stations dust of the mega traffic hub city, central China: Human health risk and relationship with black carbon.

Twenty dust samples collected from Wuchang and Wuhan Railway Stations, the biggest transport stations in the mega traffic hub city in Central China, were analyzed for polycyclic aromatic hydrocarbons (PAHs) to investigate the concentration, sources apportionment, and relationship with black carbon (BC) and assess the health risk. The results suggested that the concentrations of PAHs, BC and TOC in Wuhan Railway Station (WHRS) (PAHs = 5940 ± 1920 ng g-1, BC = 53.2 ± 23.1 mg g-1 and TOC = 80.7 ± 44.4) were twice higher than those in Wuchang Railway Station (WCRS) (PAHs = 2580 ± 1630 ng g-1, BC = 20.4 ± 14.3 mg g-1 and TOC = 33.9 ± 20.1 mg g-1). Moreover, the 3 - and 4 - rings PAHs were major PAHs in railway station dust. The composition pattern of PAHs in these railway station dusts had a common characteristic with HMW-PAHs contribution. The results of source identification revealed that different local development features and energy consumption of trains would influence the sources of PAHs and BC. PAHs and BC were most likely related to industrial activities in WHRS. Coal and biomass combustion may influence the PAHs components and BC distribution in WCRS. Moreover, BC had played an important role in retaining PAHs in urban railway stations. Especially in WHRS, BC would more likely to absorb the high molecular weight PAHs, such as 4 -ring (p<0.05), 5 -ring (p<0.05) and 6 -ring (p<0.05) PAHs; while BC just played limited roles in the binding of volatile and semi-volatile organic pollutants, such as 2 -ring and 3 -ring PAHs. With the coexistence of BC and PAHs, passengers would face significant potential health risks by exposure to toxic dust in railway stations, especially for children. The cancer risk in WHRS was almost twice higher than that in WCRS, and it would tend to be stable by a semi-confined structure in the platform area.

Behavior, remediation effect and toxicity of nanomaterials in water environments.

In recent years, nanotechnology has been developing continuously. Due to their advantageous huge specific surface areas, microinterface characteristics, remediation ability and potential environmental risks, nanomaterials have become a hot topic in the field of environmental research. With the mass production and use of nanomaterials, they will inevitably be discharged or leaked into the water environment. In this paper, we will describe some typical nanomaterials, such as nanoscale zero valent iron (nZVI), graphene nanomaterials (GNMs), TiO2 nanoparticles (NPs), ZnO NPs, Fe3O4 NPs, carbon nanotubes (CNTs), Ag NPs, and other nanomaterials in water environments, focusing on the positive and negative effects of some nanomaterials in water environments. The remediation function and the impact of nanomaterials in water environments, including behavior of nanomaterials and their toxicity to aquatic organisms will be discussed. This will be of great significance for our subsequent research on nanomaterials.

[Effects of activation of mitochondrial aldehyde dehydrogenase 2 on inflammasome production in high glucose induced A549 cells].

OBJECTIVE: To investigate the effects of activation of mitochondrial aldehyde dehydrogenase 2(ALDH2) on high glucose-induced inflammasome production in alveolar epithelial A549 cells. METHODS: The alveolar epithelial A549 cells were cultured with 25 mmol/L high glucose complete medium and divided into 4 groups: Control group, ALDH2 agonist 20 µmol/L Alda-1 group, ALDH2 antagonist 60 µmol/L Daidzin group, 20 µmol/L Alda-1 + 60 µmol/L Daidzin group. After the cells treated for 24 h, the cell proliferation activity was measured by thiazolyl blue tetrazolium bromide(MTT) colorimetric assaymethod, and the cellular reactive oxygen species(ROS) level were detected by dihydroethidium(DHE) fluorescent staining method, the cell migration ability was performed by cell scratching experiments, the protein expressions of ALDH2 and the core components of inflammasome, nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC) and cysteinyl aspartate specific protease-1(caspase-1) were detected by western blot. RESULTS: Compared with the control group, after Alda-1 activated ALDH2 specifically, the cell proliferation activity did not change significantly, but the oxidative stress level and cell migration rate were significantly decreased(P<0.05). ALDH2 protein expression was significantly increased(P<0.05), the protein expressions of NLRP3, ASC and caspase-1 were significantly decreased(P<0.05). After Daidzin blocked ALDH2 specifically, there were no significant changes in cell proliferation, oxidative stress, cell migration rate, ALDH2 and ASC protein expressions, while NLRP3 protein expression was significantly increased(P<0.05), and caspase-1 protein expression was significantly decreased(P<0.05). Compared with Alda-1 group, there was no significant changes in cell proliferation and oxidative stress in Alda-1+Daidzin group, cell migration rate was significantly increased(P<0.05), ALDH2 protein expression was decreased(P<0.05), and the protein expressions of NLRP3, ASC and caspase-1 were significantly increased(P<0.05). CONCLUSION: Increasing ALDH2 expression in alveolar epithelial A549 cells may attenuate high glucose-induced cellular inflammatory reaction, possibly through reducing cellular ROS level and reducing inflammasome expression.

Load-bearing columns inspired fabrication of ductile and mechanically enhanced BSA hydrogels.

Currently, protein-based hydrogels are widely applied in soft materials, tissue engineering and implantable scaffolds owing to their excellent biocompatibility, and degradability. However, most protein-based hydrogels are soft brittle. In this study, a ductile and mechanically enhanced bovine serum albumin (BSA) hydrogel is fabricated by soaking the a 1-(3-dimethylaminopropyl)-3ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) induced BSA hydrogel in (NH4)2SO4 solution. An EDC/NHS coupling reaction induce protein coupling reactions that cause the BSA skeleton to resemble architectural load-bearing walls, protecting the integrity of the hydrogel and preventing collapse. The effects of the BSA and (NH4)2SO4 concentrations on the hydrogel mechanics are evaluated, and the possible strengthening mechanism is discussed. Besides, the highly kosmotropic ions greatly enhance the hydrophobic interaction within BSA gels and dehydration effect and their mechanical properties were significantly enhanced. The various mechanical properties of hydrogels can be regulated over a large window by soaking hydrogels into various ions. And most of them can be washed away, maintaining high biocompatibility of the protein. Importantly, the protein hydrogels prepared by this strategy could also be modified as strain sensors. In a word, this work demonstrates a new, universal method to provide multi-functional, biocompatible, strength enhanced and regulable mechanical pure protein hydrogel, combining the Hofmeister effect with -NH2/-COOH association groups.

Drug-loaded zein nanofibers prepared using a modified coaxial electrospinning process.

This study investigated the preparation of drug-loaded fibers using a modified coaxial electrospinning process, in which only unspinnable solvent was used as sheath fluid. With zein/ibuprofen (IBU) co-dissolving solution and N, N-dimethylformamide as core and sheath fluids, respectively, the drug-loaded zein fibers could be generated continuously and smoothly without any clogging of the spinneret. Field emission scanning electron microscopy and transmission electron microscopy observations demonstrated that the fibers had ribbon morphology with a smooth surface. Their average diameters were 0.94±0.34 and 0.67±0.21 µm when the sheath-to-core flow rate ratios were taken as 0.11 and 0.25, respectively. X-ray diffraction and differential scanning calorimetry verified that IBU was in an amorphous state in all fiber composites. Fourier transform infrared spectra showed that zein had good compatibility with IBU owing to hydrogen bonding. In vitro dissolution tests showed that all the fibers could provide sustained drug release files via a typical Fickian diffusion mechanism. The modified coaxial electrospinning process reported here can expand the capability of electrospinning in generating fibers and provides a new manner for developing novel drug delivery systems.

Functional extreme learning machine for regression and classification.

Although Extreme Learning Machine (ELM) can learn thousands of times faster than traditional slow gradient algorithms for training neural networks, ELM fitting accuracy is limited. This paper develops Functional Extreme Learning Machine (FELM), which is a novel regression and classifier. It takes functional neurons as the basic computing units and uses functional equation-solving theory to guide the modeling process of functional extreme learning machines. The functional neuron function of FELM is not fixed, and its learning process refers to the process of estimating or adjusting the coefficients. It follows the spirit of extreme learning and solves the generalized inverse of the hidden layer neuron output matrix through the principle of minimum error, without iterating to obtain the optimal hidden layer coefficients. To verify the performance of the proposed FELM, it is compared with ELM, OP-ELM, SVM and LSSVM on several synthetic datasets, XOR problem, benchmark regression and classification datasets. The experimental results show that although the proposed FELM has the same learning speed as ELM, its generalization performance and stability are better than ELM.

Functional extreme learning machine.

Introduction: Extreme learning machine (ELM) is a training algorithm for single hidden layer feedforward neural network (SLFN), which converges much faster than traditional methods and yields promising performance. However, the ELM also has some shortcomings, such as structure selection, overfitting and low generalization performance. Methods: This article a new functional neuron (FN) model is proposed, we takes functional neurons as the basic unit, and uses functional equation solving theory to guide the modeling process of FELM, a new functional extreme learning machine (FELM) model theory is proposed. Results: The FELM implements learning by adjusting the coefficients of the basis function in neurons. At the same time, a simple, iterative-free and high-precision fast parameter learning algorithm is proposed. Discussion: The standard data sets UCI and StatLib are selected for regression problems, and compared with the ELM, support vector machine (SVM) and other algorithms, the experimental results show that the FELM achieves better performance.

Analysis of heavy metals in the conversion of lake sediment and restaurant waste by black soldier fly (Hermetia illucens).

The risk posed by heavy metals makes it difficult to dispose of sediment contaminants from dredging lakes in China. Black soldier fly (Hermetia illucens) can convert organic waste, such as restaurant waste and lake sediment, to high-value-added protein feed and fertilizer. Experimental groups were formed in this study to explore the conversion of heavy metals present in the mixture of restaurant waste and lake sediment by black soldier fly larvae (BSFL). The results demonstrated that BSFL could survive in pure sediment with an 84.76% survival rate. Relative to the substrate, BSFL could accumulate 70-90% zinc (Zn), chromium (Cr), copper (Cu), and 20-40% cadmium (Cd) and lead (Pb). The experimental group 2:3, with 40% lake sediment and 60% restaurant waste, was the best group after conversion for 15 days, which showed a 95.24% survival rate of BFSL, 82.20 mg average weight of BFSL, 8.92 mm average length of BFSL, with varying content of heavy metals such as Cu (43.22 mg/kg), Zn (193.31 mg/kg), Cd (1.58 mg/kg), Cr (25.30 mg/kg) Cr, and Pb (38.59 mg/kg) in BSFL. Furthermore, the conversion residue conforms to the relevant standards of organic fertilizer in China and can be used as organic fertilizer. Overall, the present study shows that black soldier flies can improve the resource utilization of lake sediment, especially by reducing the effect of heavy metals.

Tetrastigma polysaccharide reprogramming of tumor-associated macrophages via PPARγ signaling pathway to play antitumor activity in breast cancer.

ETHNOPHARMACOLOGICAL RELEVANCE: Tetrastigma Hemsleyanum Diels et Gilg (SYQ) is a typical She ethnomedicine that has been used in anti-tumor treatment in Chinese folklore. The polysaccharide of SYQ (SYQ-PA) has been reported to have antioxidant and anti-inflammatory effects, but the effect and mechanism on antitumor is still unclear. AIM OF THE STUDY: To investigate the activity and mechanism of SYQ-PA against breast cancer in vitro and in vivo. MATERIALS AND METHODS: In this study, different stages of MMTV-PYMT mice, which at 4-week-old and 8-week-old representative the transition from hyperplasia to late carcinoma, were used to investigate the potential effect of SYQ-PA of breast cancer development in vivo. The mechanism was explored with IL4/13-induced peritoneal macrophages model. Flow cytometry assay was employed to analysis the change of tumor microenvironment and the macrophages typing. The inhibition of the condition medium from macrophages on breast cancer cells was detected with xCELLigence system detection. The inflammation factors were tested with cytometric bead array. Co-culture system was used to detect the cell migration and invasion. In addition, the underlying mechanism was investigated using RNAseq analysis, Q-PCR and Western blot, and the PPARγ inhibitor was used to verify the mechanism. RESULTS: SYQ-PA significantly attenuated the process of breast primary tumor growth and reduced the infiltration of TAMs accompanied promoting the polarization of M1 phenotype in MMTV-PyMT mice. Then in vitro studies showed that SYQ-PA promoted macrophages polarization form IL4/13 induced M2 toward to the anti-tumor M1 phenotypes, and the conditioned medium (CM) from the induced macrophages inhibited the proliferation of breast cancer cells. At the same time, SYQ-PA treated macrophages inhibited the migration and invasion of 4T1 in the co-culture system. Further results indicated that SYQ-PA suppressed the release of anti-inflammatory factors and promoted the production of inflammatory cytokines which may induce M1 macrophage polarization and inhibit breast cancer cell proliferation. Subsequently, the underlying mechanism analysis based on RNAseq and molecular assays indicated that SYQ-PA inhibited PPARγ expression and regulated downstream NF-κB in macrophages. After treated with PPARγ inhibitor, T0070907, the effect of SYQ-PA was decreased, or even disappeared. As the downstream, the expression of ß-catenin was also inhibited obviously, those above all contribute the process of SYQ-PA induced M1 macrophages polarization. CONCLUSIONS: Collectively, SYQ-PA was observed inhibited breast cancer, at least in part, via PPARγ activation- and ß-catenin-mediated M2 macrophages polarization. These data expound the antitumor effect and mechanism of SYQ-PA, and provide a possible that SYQ-PA can be used as an adjuvant drug for macrophage tumor immunotherapy in breast cancer.

Enhancement of Energy Storage Performance of PMMA/PVDF Composites by Changing the Crystalline Phase through Heat Treatment.

In today's contemporary civilization, there is a growing need for clean energy focused on preserving the environment; thus, dielectric capacitors are crucial equipment in energy conversion. On the other hand, the energy storage performance of commercial BOPP (Biaxially Oriented Polypropylene) dielectric capacitors is relatively poor; hence, enhancing their performance has drawn the attention of an increasing number of researchers. This study used heat treatment to boost the performance of the composite made from PMAA and PVDF, combined in various ratios with good compatibility. The impacts of varying percentages of PMMA-doped PMMA/PVDF mixes and heat treatment at varying temperatures were systematically explored for their influence on the attributes of the blends. After some time, the blended composite's breakdown strength improves from 389 kV/mm to 729.42 kV/mm at a processing temperature of 120 °C. Consequently, the energy storage density is 21.12 J/cm3, and the discharge efficiency is 64.8%. The performance has been significantly enhanced compared to PVDF in its purest state. This work offers a helpful technique for designing polymers that perform well as energy storage materials.

Research on the application of the blended BOPPPS based on an online and offline mixed teaching model in the course of fermentation engineering in applied universities.

This study aimed to investigate the teaching effect of the blended BOPPPS based on an online and offline mixed teaching model ("B + BOPPPS") in the course of fermentation engineering in applied universities. The participants were 142 undergraduates majoring from the course of fermentation engineering in Food Science and Engineering in 2019 and 2020 in Huanghuai University, Zhumadian city, Henan province, China. The students in the control group (68 students) were taught in 2019, and the students in the experimental group (74 students) were taught in 2020. The traditional teaching method and "B + BOPPPS" were implemented, respectively. The teaching effect was evaluated using the questionnaire survey of course satisfaction and theoretical knowledge test. The results showed that the scores of the theoretical knowledge test in the experimental group adopting "B + BOPPPS" were significantly higher than those in the control group, and the difference was statistically significant (p < 0.01). The students had a good evaluation of the "B + BOPPPS" in many aspects, which included achieving learning goals, providing in-depth understanding of knowledge points, stimulating interest in learning, training in the ability to analyze and think about problems, and so on. The results suggested that "B + BOPPPS" could stimulate students' interest in learning and improve their subjective initiative. They could also improve students' ability to master and apply knowledge, which was conducive to improving the theoretical teaching quality of the course of fermentation engineering.

Panax notoginseng saponins normalises tumour blood vessels by inhibiting EphA2 gene expression to modulate the tumour microenvironment of breast cancer.

BACKGROUND: Panax notoginseng saponins (PNS), the main active component of Panax notoginseng, can promote vascular microcirculation. PNS exhibits antitumor effects in various cancers. However, the molecular basis of the relationship between PNS and tumor blood vessels remains unclear. PURPOSE: To study the relationship between PNS inhibiting the growth and metastasis of breast cancer and promoting the normalization of blood vessels. METHODS: We performed laser speckle imaging of tumor microvessels and observed the effects of PNS on tumor growth and metastasis of MMTV-PyMT (FVB) spontaneous breast cancer in a transgenic mouse model. Immunohistochemical staining of Ki67 and CD31 was performed for tumors, scanning electron microscopy was used to observe tumor vascular morphology, and flow cytometry was used to detect tumor tissue immune microenvironment (TME). RNA-seq analysis was performed using the main vessels of the tumor tissues of the mice. HUVECs were cultured in tumor supernatant in vitro to simulate tumor microenvironment and verify the sequencing differential key genes. RESULTS: After treatment with PNS, we observed that tumor growth was suppressed, the blood perfusion of the systemic tumor microvessels in the mice increased, and the number of lung metastases decreased. Moreover, the vascular density of the primary tumor increased, and the vascular epidermis was smoother and flatter. Moreover, the number of tumor-associated macrophages in the tumor microenvironment was reduced, and the expression levels of IL-6, IL-10, and TNF-α were reduced in the tumor tissues. PNS downregulated the expression of multiple genes associated with tumor angiogenesis, migration, and adhesion. In vitro tubule formation experiments revealed that PNS promoted the formation and connection of tumor blood vessels and normalized the vessel morphology primarily by inhibiting EphA2 expression. In addition, PNS inhibited the expression of tumor vascular marker proteins and vascular migration adhesion-related proteins in vivo. CONCLUSION: In this study, we found that PNS promoted the generation and connection of tumor vascular endothelial cells, revealing the key role of EphA2 in endothelial cell adhesion and tumor blood vessel morphology. PNS can inhibit the proliferation and metastasis of breast cancer by inhibiting EphA2, improving the immune microenvironment of breast cancer and promoting the normalization of tumor blood vessels.

Fabricating Oxidized Cellulose Sponge for Hemorrhage Control and Wound Healing.

Uncontrolled hemorrhage and infection are the main reasons for many trauma-related deaths in both clinic and battlefield. However, most hemostatic materials have various defects and side effects, such as low hemostatic efficiency, poor biocompatibility, weak degradation ability, and lack of antimicrobial properties. Herein, an oxidized cellulose (OC) sponge with antibacterial properties and biosafety was fabricated for hemorrhage control and wound healing. The as-prepared OC sponges were prone to water triggered expansion and superabsorbent capacity, which could facilitate blood component concentration effectively. Importantly, they had significant biodegradability with little irritation to the skin. This hemostat could also reduce the plasma clotting time to 53.54% in vitro and demonstrated less blood loss than commercially available hemostatic agents (GS) in a mouse model of bleeding from liver defects. Furthermore, the biocompatibility antimicrobial properties and possible hemostatic mechanism of the OC sponge were also systematically evaluated. Importantly, the potential wound healing applications have also been demonstrated. Therefore, the materials have broad clinical application prospects.