Puerarin enhances TFEB-mediated autophagy and attenuates ROS-induced pyroptosis after ischemic injury of random-pattern skin flaps.

BACKGROUND AND AIM: Necrosis of random-pattern flaps restricts their application in clinical practice. Puerarin has come into focus due to its promising therapeutic effects in ischemic diseases. Here, we employed Puerarin and investigated its role and potential mechanisms in flap survival. EXPERIMENTAL PROCEDURE: The effect of Puerarin on the viability of human umbilical vein endothelial cells (HUVECs) was assessed by CCK-8, EdU staining, migration, and scratch assays. Survival area measurement and laser Doppler blood flow (LDBF) were utilized to assess the viability of ischemic injury flaps. Levels of molecules related to oxidative stress, pyroptosis, autophagy, transcription factor EB (TFEB), and the AMPK-TRPML1-Calcineurin signaling pathway were detected using western blotting, immunofluorescence, dihydroethidium (DHE) staining, RT-qPCR and Elisa. KEY RESULTS: The findings demonstrated that Puerarin enhanced the survivability of ischemic flaps. Autophagy, oxidative stress, and pyroptosis were implicated in the ability of Puerarin in improving flap survival. Increased autophagic flux and augmented tolerance to oxidative stress contribute to Puerarin's suppression of pyroptosis. Additionally, Puerarin modulated the activity of TFEB through the AMPK-TRPML1-Calcineurin signaling pathway, thereby enhancing autophagic flux. CONCLUSIONS AND IMPLICATIONS: Puerarin promoted flap survival from ischemic injury through upregulation of TFEB-mediated autophagy and inhibition of oxidative stress. Our findings offered valuable support for the clinical application of Puerarin in the treatment of ischemic diseases, including random-pattern flaps.

Experimental and Numerical Investigation of Axial Compression Behaviour of FRP-Confined Concrete-Core-Encased Rebar.

The axial compression behaviour of fibre-reinforced polymer (FRP)-confined concrete-core-encased rebar (FCCC-R) was investigated by performing monotonic axial compression tests on seven groups of FCCC-R specimens and three groups of pure rebar specimens. The research parameters considered were the FRP winding angle (0°, ±45°, and 90°), number of layers (2, 4, and 6 layers), and slenderness ratio of specimens (15.45, 20, and 22.73). The test results showed that FCCC-R's axial compression behaviour improved significantly compared with pure rebar. The axial load-displacement curves of the FCCC-R specimens had a second ascending branch, and their carrying capacity and ductility were enhanced substantially. The best buckling behaviour was observed for the FRP winding angle of 90°. The capacity and ductility of the specimens were positively related to the number of FRP-wrapped layers and inversely related to the slenderness ratio of the specimens. A finite element model of FCCC-R was constructed and agreed well with the test results. The finite element model was used for parametric analysis to reveal the effect of the area ratio, FRP confinement length, internal bar eccentricity, and mortar strength on the axial compression behaviour of FCCC-R. The numerical results showed that the area ratio had the most significant impact on the axial compression behaviour of FCCC-R. The confinement length of the FRP pipe and internal bar eccentricity had similar effects on the axial compression behaviour of FCCC-R. Both of them had a significant impact on the second ascending branch, with the post-peak behaviour exhibiting minimal differences. The influence of mortar strength on the axial compression behaviour of FCCC-R was observed to be minimal.

Research on Hysteretic Behavior of FRP-Confined Concrete Core-Encased Rebar.

FRP-confined concrete core-encased rebar (FCCC-R) is a novel composite structure that has recently been proposed to effectively delay the buckling of ordinary rebar and enhance its mechanical properties by utilizing high-strength mortar or concrete and an FRP strip to confine the core. The purpose of this study was to study the hysteretic behavior of FCCC-R specimens under cyclic loading. Different cyclic loading systems were applied to the specimens and the resulting test data were analyzed and compared, in addition to revealing the mechanism of elongation and mechanical properties of the specimens under the different loading systems. Furthermore, finite-element simulation was performed for different FCCC-Rs using the ABAQUS software. The finite-element model was also used for the expansion parameter studies to analyze the effects of different influencing factors, including the different winding layers, winding angles of the GFRP strips, and the rebar-position eccentricity, on the hysteretic properties of FCCC-R. The test result indicates that FCCC-R exhibits superior hysteretic properties in terms of maximum compressive bearing capacity, maximum strain value, fracture stress, and envelope area of the hysteresis loop when compared to ordinary rebar. The hysteretic performance of FCCC-R increases as the slenderness ratio is increased from 10.9 to 24.5 and the constraint diameter is increased from 30 mm to 50 mm, respectively. Under the two cyclic loading systems, the elongation of the FCCC-R specimens is greater than that of ordinary rebar specimens with the same slenderness ratio. For different slenderness ratios, the range of maximum elongation improvement is about 10% to 25%, though there is still a large discrepancy compared to the elongation of ordinary rebar under monotonic tension. Despite the maximum compressive bearing capacity of FCCC-R is improved under cyclic loading, the internal rebars are more prone to buckling. The results of the finite-element simulation are in good agreement with the experimental results. According to the study of expansion parameters, it is found that the hysteretic properties of FCCC-R increase as the number of winding layers (one, three, and five layers) and winding angles (30°, 45°, and 60°) in the GFRP strips increase, while they decrease as the rebar-position eccentricity (0.15, 0.22, and 0.30) increases.

Mechanical, water resistance and environmental benefits of magnesium oxychloride cement incorporating rice husk ash.

Magnesium oxychloride cement (MOC) has received extensive attention as an eco-friendly cement, but its poor water resistance limits its engineering applications. In this study, MOC mixture (MOCM) was modified with 10-50 % rice husk ash (RHA) (wt% of MgO), and the development of their fresh properties, mechanical strength and microstructure was investigated. The results show that the incorporation of RHA to MOCM increases the setting time of the mixture and reduces its flowability. Due to the fine particle size and high reactivity of RHA, the incorporation of an appropriate amount of RHA to MOCM improves the matrix compactness, thereby enhancing the compressive strength of the samples. Although the microstructure of MOCM deteriorates and the strength decreases after immersion in water, the strength retention coefficient of MOCM with 50 % RHA increases by 24.57 % compared with that of plain MOCM. The incorporation of RHA not only reduces the relative content of magnesium oxide in MOCM, but also generates Mg-Cl-Si-H gel, which is beneficial to improve the water resistance of MOCM. Meanwhile, with the increase of RHA content, the carbon emission of MOCM also decreases. Compared with other modification methods, RHA-modified MOCM performs better in terms of water resistance, environmental benefits and strength enhancement.