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1.
Oncogene ; 13(8): 1687-92, 1996 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-8895514

RESUMO

IPW (Imprinted gene in the Prader-Willi syndrome region) is a recently identified paternally expressed gene. Previous work has demonstrated IPW expression in the human fetus and adult, with monoallelic expression in adult lymphoblasts and fibroblasts, and in fetal tissues. To further examine the expression of IPW, a series of experiments were carried out using RT-PCR to measure IPW expression in placentae and various fetal and tumor tissues. Biallelic expression of IPW was found in testicular germ cell tumor and bladder cancer cells, suggesting loss of imprinting in the latter case. Both H19 and Insulin-like growth Factor 2 (IGF2), two additional imprinted genes, also showed biallelic expression in those same tumors that demonstrated IPW biallelic expression. Of note, the naturally occurring parthenogenetic-derived mature teratoma unexpectedly expressed large amounts of IPW. Lastly, the pluripotent embryonal cancer cell line Tera-2 expressed IPW at the same level before and after differentiation induced by retinoic acid, suggesting that this gene functions in a 'housekeeping' capacity throughout cell growth. This was in contradistinction to H19 and IGF2, both of which showed significant transcriptional upregulation after Tera-2 cell differentiation.


Assuntos
Alelos , Impressão Genômica , Síndrome de Prader-Willi/genética , Adulto , Carcinoma Embrionário/genética , Carcinoma Embrionário/patologia , Feminino , Feto/metabolismo , Expressão Gênica , Humanos , Masculino , Neoplasias Embrionárias de Células Germinativas/genética , Neoplasias Embrionárias de Células Germinativas/patologia , Gravidez , Teratoma/genética , Teratoma/patologia , Neoplasias Testiculares/genética , Neoplasias Testiculares/patologia , Células Tumorais Cultivadas , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
2.
Oncogene ; 11(5): 863-70, 1995 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-7545806

RESUMO

Certain embryonal tumors demonstrate a loss of heterozygosity at the parentally imprinted region of chromosome 11p15.5. It has been hypothesized that this implicates a tumor suppressor gene at this locus. The human H19 gene maps to 11p15.5, is expressed in fetal tissues including the placenta and is paternally imprinted. Here we show that the abundance of H19 transcripts in cells of two choriocarcinoma derived cell lines (JAr and JEG-3) differs greatly. While JAr cells express high levels of H19 RNA, the expression of H19 in JEG-3 cells is much lower than that of normal trophoblasts. Cells of these two cell lines were subcutaneously injected into nude mice with subsequent tumor formation. A fivefold increase in the H19 RNA level was measured in tumors derived from JEG-3 cell lines as compared to these cells before injection. However this increase in H19 RNA did not alter the clonogenicity in soft agar nor the growth rate of the cells derived from these tumors as compared to the original JEG-3 cells. Nevertheless, the cells retaining the elevated level of H19 transcripts were more tumorigenic than the original cells. We propose that there is a selection of cells expressing high levels of H19 from the total JEG-3 cell population during the microevolution of tumor formation. These observations, together with our previous publications on H19 expression in human cancers, do not support the notion of a tumor suppressor role for the H19 gene.


Assuntos
Coriocarcinoma/genética , Genes Supressores de Tumor , Proteínas Musculares/genética , Neoplasias Ovarianas/genética , RNA não Traduzido , Animais , Sequência de Bases , Gonadotropina Coriônica/genética , Gonadotropina Coriônica Humana Subunidade beta , Feminino , Expressão Gênica , Humanos , Camundongos , Camundongos Nus , Dados de Sequência Molecular , Fragmentos de Peptídeos/genética , Gravidez , Proto-Oncogenes , RNA/análise , RNA Longo não Codificante , Neoplasias Cutâneas/genética , Transplante Heterólogo , Células Tumorais Cultivadas
3.
Urology ; 45(2): 335-8, 1995 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-7855987

RESUMO

OBJECTIVES: Genomic imprinting is a newly discovered mechanism in genetics that is involved in tumorigenesis. H19 is an imprinted gene in the human, expressed from the maternal allele. It is extensively transcribed in fetal life but is not translated and functions as an RNA molecule. It has been suggested as a candidate tumor suppressor gene. We studied the expression of H19 in human cancer arising from tissues expressing H19 in fetal life, one of which is bladder mucosa. METHODS: In situ hybridization for H19 mRNA on paraffin sections of bladder carcinoma in different histologic grades. RESULTS: Low-grade (grade 1 of 3), noninvasive (Ta) papillary transitional cell bladder carcinoma did not express H19, but prominent expression was disclosed in higher grades, invasive transitional cell carcinomas (T1-T3/4). Expression was also evident in in situ bladder carcinoma (Tis), which tends to progress rapidly to invasive cancer. CONCLUSIONS: We suggest that H19 can be used as a tumor marker in human bladder carcinoma, where its expression indicates a more malignant potential.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma de Células de Transição/genética , Regulação Neoplásica da Expressão Gênica/genética , Genes/genética , Impressão Genômica , Neoplasias da Bexiga Urinária/genética , Humanos , RNA Mensageiro/biossíntese
4.
Fertil Steril ; 62(5): 903-10, 1994 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-7523201

RESUMO

OBJECTIVES: To outline the possible role of the imprinted genes in early human embryogenesis and implantation. DATA IDENTIFICATION: Literature review. STUDY SELECTION: Studies examining the issues of genomic imprinting, implantation, gestational trophoblastic diseases, placental gene expression, and trophoblast invasion. RESULTS: Certain genes have been shown to be expressed either in the embryo or in the uterine decidua before implantation. Some of these have been shown to be parentally imprinted, that is, expressed either from their paternal or maternal origin. The paternally expressed genes are linked to placental proliferation and invasiveness. CONCLUSIONS: Clinical and basic data from different disciplines indicate that genomic imprinting may be crucial to the process of implantation.


Assuntos
Implantação do Embrião/genética , Impressão Genômica , Animais , Gonadotropina Coriônica/genética , Gonadotropina Coriônica Humana Subunidade beta , Mecanismo Genético de Compensação de Dose , Feminino , Humanos , Fator de Crescimento Insulin-Like II/genética , Masculino , Fragmentos de Peptídeos/genética
5.
Mol Reprod Dev ; 38(3): 239-46, 1994 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-7917273

RESUMO

The H19 gene is a parenterally imprinted maternally expressed gene which has a pivotal role in embryogenesis and fetal development. It is tightly linked to the IGF-II gene on chromosome 11p15.5 which is reciprocally imprinted. We studied the expression of the human H19 by in situ hybridization in an embryo 35 days post coitus (dpc) and in a fetus from the second trimester of pregnancy. The expression pattern of H19 in the human fetal tissues was similar to its expression in the mouse, and paralleled, with some exceptions, the expression of IGF-II in human fetuses. Abundant expression was found in organs comprising the fetoplacental unit: the placenta, the fetal adrenal, and liver. The expression in the fetal adrenal cortex was most prominent in the definitive cortex and somewhat weaker in the fetal zone. Considerable expression of H19 was found in the fetal liver as early as 35 dpc and in the second trimester. Hematopoietic cells in fetal liver did not express the gene. Moderate expression of H19 was detected in the epithelium of the small intestines, in endometrial stroma and Fallopian tube. In the kidney conspicuous labeling of the metanephric blastema was noted, which was markedly reduced with differentiation to tubules. This pattern of expression is identical to that of IGF-II in the fetal kidney and is relevant to the evolution of Wilms' tumor. No expression of H19 was found in the neural tube of the first trimester embryo or in the developing fetal brain in the second trimester, nor were transcripts detected in the choroid plexus.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Desenvolvimento Embrionário e Fetal/genética , Expressão Gênica , Glândulas Suprarrenais/metabolismo , Animais , Cromossomos Humanos Par 11 , Membranas Extraembrionárias/metabolismo , Feminino , Ligação Genética , Humanos , Hibridização In Situ , Fator de Crescimento Insulin-Like II/genética , Rim/metabolismo , Fígado/metabolismo , Masculino , Camundongos , Placenta/metabolismo , Gravidez , Sondas RNA , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Distribuição Tecidual , Sistema Urogenital/metabolismo
6.
Gynecol Oncol ; 53(2): 212-9, 1994 May.
Artigo em Inglês | MEDLINE | ID: mdl-8188082

RESUMO

Genomic imprinting--the uniparental-dependent transmittance of a genetic trait--has been accepted in recent years as a major mechanism in mammalian genetics. We studied the expression of the H19 gene, a parentally imprinted (maternally expressed) gene, by in situ hybridization in human placenta and trophoblastic disease. Expression was found to be abundant, in a decreasing order, in the intermediate trophoblast (villous and interstitial), the cytotrophoblast, and the syncytiotrophoblast. The villous stroma was also prominently labeled. Partial hydatidiform mole showed a similar pattern of expression as normal placenta. As expected, complete hydatidiform mole, whose genome consists of two haploid sets of paternal origin, was not labeled in the villous stroma and surrounding trophoblastic layer. However, some of the large mononuclear cells in the proliferating groups sprouting from the villous surface were strongly labeled. Prominent expression of H19 was found in placental site trophoblastic tumor and gestational choriocarcinoma. The phenomenon of emergence of expression of alleles subject to repression according to their gamete of origin is termed relaxation of imprinting, and is considered to be relevant to tumorigenesis. We suggest that the expression of the maternally expressed H19 gene in the androgenetic tissue of complete hydatidiform mole represents relaxation of imprinting and may be associated with its malignant potential.


Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias Trofoblásticas/genética , Neoplasias Uterinas/genética , Coriocarcinoma/genética , Feminino , Humanos , Mola Hidatiforme/genética , Hibridização In Situ , Gravidez , Tumor Trofoblástico de Localização Placentária/genética
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