Genetic analysis of floral organ size in broccoli × cabbage via a mixed inheritance model of a major gene plus polygene.

Broccoli and cabbage are important vegetable crops that produce hybrid seeds after insect pollination; the size of floral organs is crucial for this process. To investigate the genetic characteristics of floral organ sizes (corolla width, petal length and width, and lengths of stamen, anther, style, and stigma) and to improve the flower size and breeding efficiency of broccoli, we used multi-generation analysis of a major gene plus polygene model. Six populations obtained from a broccoli inbred line 93219 (small floral organs) and cabbage inbred line 195 (large floral organs) were used for the analysis. Corolla and petal width and stamen and anther length were controlled by the additive-dominance-epistasis polygene model. The heritability of these traits in BC1, BC2, and F2 generations was high (72.80-93.76%). Petal and stigma length were governed by the two major genes of additive-dominance-epistasis effects plus additive-dominance polygene model; the major gene heritability in the F2 generation were 79.17 and 65.77%, respectively. Style length was controlled by one major gene of additive-dominance effects plus additive-dominance-epistasis polygene model; the major gene heritability in BC1, BC2, and F2 were 40.60, 10.35, and 38.44%, respectively; the polygene heritability varied from 41.85 to 68.44%. Our results provide important genetic information for breeding, which could guide improvement of flower-related traits and lay the foundation for quantitative trait loci mapping of the flower-size traits in Brassica.

Retraction Note: LINC01296 promotes the proliferation and invasion by regulating microRNA-760 expression and predicts poor prognosis of hepatocellular carcinoma.

The article "LINC01296 promotes the proliferation and invasion by regulating microRNA-760 expression and predicts poor prognosis of hepatocellular carcinoma", by Z.-C. Wang, S. Yang, M.-Q. Chen, S.-S. Wu, H.-H. Lv, W.-X. Jin, published in Eur Rev Med Pharmacol Sci 2019; 23 (22): 9848-9856-DOI: 10.26355/eurrev_201911_19548- PMID: 31799652 has been retracted by the author for the following reasons: After the publication of this article, the authors reviewed the process of the experiment and found there were mistakes in the methodology search. Not all the carcinoma specimens used in the experiment were from hepatocellular carcinoma. The prognosis and clinical features the rate of lymph node metastasis and the treatment and prognosis between these types of cancers are different. For example, intrahepatic cholangiocarcinoma tends to involve a high frequency of lymph node metastasis, and the prognosis is worse than hepatocellular carcinoma; hepatocellular carcinoma seldom involves lymph node metastasis but often involves intrahepatic metastasis; colorectal cancer liver metastasis is far more sensitive to chemotherapy and has a relatively long survival, and neuroendocrine carcinoma is sometimes sensitive to hormone therapy and generally has better survival than hepatocellular carcinoma. As a result, the clinical results and gene test results mentioned in this article were incorrect. After that the authors found that there was a mistake, they subsequently carried out supplementary experiments and found that they could not confirm that there was an increase in the expression of the LICO01296 gene in hepatocellular carcinoma, so they could not link some clinical results with the results of the basic research results mentioned in this article. Therefore, from the perspective of academic rigor, they requested to withdraw the article. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/19548.

LINC01296 promotes the proliferation and invasion by regulating microRNA-760 expression and predicts poor prognosis of hepatocellular carcinoma.

OBJECTIVE: The aim of this study was to investigate the role of LINC01296 in the progression of liver cancer (LCa) and to explore its possible molecular mechanisms. PATIENTS AND METHODS: TCGA database was used for information mining to verify the expression of LINC01296 in liver tumor tissues and normal tissues. The levels of LINC01296 in 40 pairs of LCa and adjacent tissues, as well as normal liver cell lines and liver cancer cell lines, were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The Chi-square test was performed to analyze the clinical characteristics of tumor samples and LINC01296 expression. Meanwhile, the Chi-square test was used to explore the relationship between different clinical indicators and the expression of LINC01296. The liver cancer cell lines were screened and transfected with siRNA-LINC01296 and microRNA-760 inhibitor, as well as corresponding negative controls, respectively. Cell counting kit-8 (CCK-8) and Transwell assays were used to determine the effects of LINC01296 on the proliferative and invasive capacities of cells. Furthermore, the regulatory association between LINC01296 and microRNA-760 was verified by the Dual-Luciferase reporter assay. RESULTS: LINC01296 expression was remarkably higher in LCa tissues than that of normal liver tissues. Meanwhile, LINC01296 expression was associated with poor prognosis of LCa. Patients with high LINC01296 expression were more likely to have lymph node metastasis. In vitro experiments showed that the knockdown of LINC01296 significantly inhibited the proliferation and migration of HCC cells. Meanwhile, microRNA-760 was remarkably lowly expressed in LCa tissues and cells. Subsequent experiments indicated that LINC01296 was regulated by miR760 in LCa tissues, and high expression of linc0129 could limit microRNA-760 expression. Furthermore, the inhibition of microRNA-760 in HCC cells reversed the effect of LINC01296 knockdown on cell proliferation and invasion. CONCLUSIONS: LINC01296 could promote the proliferative ability and invasiveness of hepatoma cells by inhibiting the expression of microRNA-760. Moreover, its expression was closely related to lymph node metastasis and poor prognosis of LCa.