RESUMO
BACKGROUND: Nanotechnology has been widely applied in agricultural science. During the process of reducing metal toxicity and accumulation in rice, nanomaterials exhibit size effects. However, there is limited knowledge regarding these size effects. We aim to explore the impact of fertilizer with various sizes of ZnO nanoparticles (ZnO-NPs) on rice growth and cadmium (Cd) accumulation and to elucidate the potential mechanism of Cd reduction in rice. Foliar applications of different concentrations (0.5 and 2 mmol L-1) and different sizes (30 and 300 nm ZnO-NPs) of zinc (Zn) fertilizer (Zn(NO3)2) were performed to investigate the effects on rice growth, Cd accumulation and subcellular distribution, and the expression of Zn-Cd transport genes. RESULTS: The results suggested that all the foliar sprayings can significantly reduce the Cd concentrations in rice grains by 41-61% with the highest reduction in the application of ZnO-NPs with large size and low concentration. This is related to the enhancement of Cd fixation in leaf cell walls and downregulation of Cd transport genes (OsZIP7, OsHMA2, OsHMA3) in stem nodes. Foliar ZnO-NPs applications can increase the Zn concentration in grains by 9-21%. Foliar applications of Zn(NO3)2 and small-sized ZnO-NPs promoted plant growth and rice yield, while the application of large-sized ZnO-NPs significantly reduced rice growth and yield. CONCLUSION: The study suggests that the rice yield and Cd reduction are dependent on the size and concentration of foliar spraying and the use of large-sized ZnO-NPs is the most effective strategy when considering both yield and Cd reduction comprehensively. © 2024 Society of Chemical Industry.
RESUMO
The majority of bioactive substances in the human diet come from polyphenols. Here, we use spectroscopy, molecular docking, molecular dynamics simulations, and in vitro digestion to look at the relationship between caffeine (CAF) and bovine lactoferrin (BLF). The correlation analysis of the CAF-BLF fluorescence quenching process revealed that the reaction was spontaneous and that the CAF-BLF fluorescence quenching process may have been static. The predominant intrinsic binding forces were hydrogen bonds and van der Waals forces, which were also supported by molecular docking and molecular dynamics simulations. Through Fourier infrared and circular dichroism spectroscopy experiments, it was found that CAF changed the secondary structure of BLF and might bind to the hydrophobic amino acids of BLF. Compared with BLF, CAF-BLF showed inhibitory effects on digestion in simulated in vitro digestion. It will be helpful to better understand the interaction between CAF and BLF and provide the basis for the development of innovative dairy products.
Assuntos
Cafeína , Lactoferrina , Cafeína/química , Cafeína/metabolismo , Lactoferrina/química , Lactoferrina/metabolismo , Simulação de Acoplamento Molecular , Bovinos , Técnicas In Vitro , Digestão , Animais , Humanos , Espectroscopia de Infravermelho com Transformada de Fourier , Dicroísmo Circular , Simulação de Dinâmica Molecular , LaticíniosRESUMO
In this study, we explored the effects of combining goat milk and oligosaccharides on the large intestine environment of mice. A combination of goat milk with each of 3 oligosaccharides-stachyose, fructo-oligosaccharide (FOS), and a prebiotics mix-were independently fed to mice. We investigated composition changes in the microbiota of the large intestine using 16S rRNA gene sequencing; measured short-chain fatty acid content using gas chromatography-mass spectrometry; and performed a Spearman correlation analysis between microorganisms and short-chain fatty acids. Our results showed that microbial diversity in the large intestine decreased significantly in the FOS group. In terms of α diversity, microbial richness significantly declined in all 3 treatment groups; in terms of ß diversity, the intestinal microbial structures clearly changed in the FOS group. The abundance of Bifidobacterium and Lactobacillus increased markedly in the FOS group compared with the other groups. Functional predictions showed that FOS reduced intestinal bacterial infections and improved the endocrine and immune systems. Spearman correlation analysis showed that propionic, isobutyric, and valeric acids were all positively correlated with certain microbiota. Our findings suggest that FOS-enriched goat milk is beneficial for improving the large intestine environment in the host.
Assuntos
Microbiota , Leite , Animais , Ácidos Graxos Voláteis , Fezes , Cabras , Intestino Grosso , Camundongos , Oligossacarídeos , Prebióticos , RNA Ribossômico 16S/genéticaRESUMO
Nanoplastic pollution poses a significant global concern for public health due to the potential toxicity it induces in the human body through food and water intake. Consequently, the urgent task of removing nanoplastics, especially from water resources, is paramount for enhancing food safety, and developing eco-friendly materials capable of efficiently removing nanoplastics is crucial. In this context, we propose the use of biodegradable anionic seaweed cellulose nanofibers (TEMPO-mediated seaweed cellulose nanofibers, TCNFs) and cationic seaweed cellulose nanofibers (quaternized seaweed cellulose nanofibers, QCNFs) for nanoplastic removal in both single- and copollutant systems. In our experiments under simulated practical conditions, we revealed that TCNFs and QCNFs achieved an average removal efficiency of 98.71% against nanoplastic particles. Moreover, TCNFs and QCNFs exhibited higher adsorption capacities compared to those of existing materials, potentially offering a cost-effective advantage. Toxicity assessments conducted with mammalian cells further confirmed the biosafety of TCNFs and QCNFs. This study contributes to the scientific and theoretical understanding of using edible seaweed as well as offers promising solutions for food safety control in an efficient, cost-effective, and eco-friendly manner.
RESUMO
The Internet contains a wealth of public opinion on food safety, including views on food adulteration, food-borne diseases, agricultural pollution, irregular food distribution, and food production issues. To systematically collect and analyze public opinion on food safety in Greater China, we developed IFoodCloud, which automatically collects data from more than 3,100 public sources. Meanwhile, we constructed sentiment classification models using multiple lexicon-based and machine learning-based algorithms integrated with IFoodCloud that provide an unprecedented rapid means of understanding the public sentiment toward specific food safety incidents. Our best model's F1 score achieved 0.9737, demonstrating its great predictive ability and robustness. Using IFoodCloud, we analyzed public sentiment on food safety in Greater China and the changing trend of public opinion at the early stage of the 2019 Coronavirus Disease pandemic, demonstrating the potential of big data and machine learning for promoting risk communication and decision-making.
RESUMO
The present study investigated effects of the fermented goat milk (FGM) produced with a combination of Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. bulgaricus (SL) and with another one of SL as well as Bifidobacterium animalis subsp. lactis (SLB) on the internal environment in the large intestine of mice. Both the richness and the diversity of microbiota was the most abundant in SL group. SL, SLB and the control groups were clustered into three independent groups. At the phylum level, Firmicutes was the most abundant in all the groups, and Deferribacteres in SLB group was more abundant than in SL group. SL and SLB increased the proportions of 6 and 5 genera, respectively. Firmicutes was positively correlated with all the significantly different species but negative to Actinobacteria at the phylum level. SL and SLB both significantly decreased the levels of butyric and valeric acids. Either SL or SLB can significantly improve the structures of microbiota, and both of them significantly decreased the levels of butyric and valeric acids in the large intestine of mice. Certain microbiota negatively related to the levels of butyric and valeric acids. FGM produced with SL or SLB can effectively alter the microbialflora and the levels of SCFA in the large intestine of mice.
Assuntos
Bifidobacterium animalis , Produtos Fermentados do Leite , Lactobacillus delbrueckii , Microbiota , Animais , Produtos Fermentados do Leite/microbiologia , Ácidos Graxos Voláteis , Cabras , Intestino Grosso , Ácido Láctico , Camundongos , Streptococcus thermophilusRESUMO
Perovskite-type proton-conducting materials, such as BaCe0.7Zr0.1Y0.1Yb0.1O3-δ (BCZYYb), are very attractive for the next-generation equipment of electrochemical energy conversion and storage owing to their excellent conductivity in the intermediate-temperature range (300-750 °C), as well as good thermo-chemical stability, coking resistance, and sulfur tolerance. However, the lack of a reliable and cost-effective synthesis method for such multi-component co-doping oxides limits their large-scale application. In this study, for the first time, we successfully synthesize BCZYYb electrolyte nanopowders by using a rapid, scalable flame-based gas-phase synthesis method with two different barium precursors Ba(NO3)2 and Ba(CH3COO)2, named as BCZYYb (N) and BCZYYb (CA). The as-synthesized nanoparticles exhibit good crystallinity of the pure orthorhombic perovskite BCZYYb phase. BCZYYb (CA) shows more uniform doping with the element ratio of 1:0.74:0.12:0.08:0.1, which is very close to the theoretical value. The shrinkage and surface SEM (scanning electron microscope) results indicate that the flame-made powders have superior sinterability compared to the sol-gel-made powders because of the smaller primary particle size (â¼20 nm). Electrochemical impedance spectroscopy tests show that BCZYYb (CA) sintered at 1450 °C has the highest protonic conductivity of 1.31 × 10-2 S cm-1 in wet H2 when operating at 600 °C and still maintains a high-level conductivity of 1.19 × 10-2 S cm-1 even when the sintering temperature is reduced to 1350 °C, which is mainly attributed to uniform doping and good sinterability. The activation energy for the conductivity of BCZYYb (CA) is also significantly lower than that of conventional electrolytes, which suggests much better conductivity in the intermediate (â¼600 °C) and even lower operating temperature. The excellent conductivity performance combined with the high-throughput production capability makes the swirling spray flame a promising synthesis method for promoting the BCZYYb electrolytes from lab to industrial-scale solid oxide fuel cells.
RESUMO
Goat milk and oligosaccharides play important roles in gastrointestinal health. A combination of goat milk with three oligosaccharides, stachyose (STS), fructo-oligosaccharide (FOS), and a prebiotics mixture (FGS), was fed to mice. Changes and functions of the microbiota, short chain fatty acid (SCFA) concentrations, and immune gene expression in the small intestines were determined. The FOS treatment increased the beneficial bacteria Lactobacillus and Bifidobacterium, the FGS treatment helped stabilize the microbial community, and the STS treatment significantly enhanced microbial diversity and the growth of Bacteroidetes. The oligosaccharide treatments regulated the gene expression levels of the immune factors tumor necrosis factor alpha (Tnfα), granzyme B (Gzmb), perforin (Prf), and aryl hydrocarbon receptor (Ahr). Stachyose significantly increased the concentrations of acetate and propionate compared with other treatments. These findings demonstrate that STS is the preferred carbon source for microbiota, slightly modulates SCFA production, and results in low immunogenicity in the small intestines of mice.