RESUMO
A flexible panel consisting of 38 informative microsatellite markers for Salmo trutta is described. These markers were selected from a pool of over 150 candidate loci that can be readily amplified in four multiplex PCR groups but other permutations are also possible. The basic properties of each markers were assessed in six population samples from both the Burrishoole catchment, in the west of Ireland, and Lough Neagh, in Northern Ireland. A method to assess the relative utility of individual markers for the detection of population genetic structuring is also described. Given its flexibility, technical reliability and high degree of informativeness, the use of this panel of markers is advocated as a standard for S. trutta genetic studies.
Assuntos
Repetições de Microssatélites , Truta/genética , Animais , Variação Genética , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Irlanda , Reação em Cadeia da Polimerase/métodos , Truta/classificaçãoRESUMO
People universally recognize facial expressions of happiness, sadness, fear, anger, disgust, and perhaps, surprise, suggesting a perceptual mechanism tuned to the facial configuration displaying each emotion. Sets of drawings were generated by computer, each consisting of a series of faces differing by constant physical amounts, running from one emotional expression to another (or from one emotional expression to a neutral face). Subjects discriminated pairs of faces, then, in a separate task, categorized the emotion displayed by each. Faces within a category were discriminated more poorly than faces in different categories that differed by an equal physical amount. Thus emotional expressions, like colors and speech sounds, are perceived categorically, not as a direct reflection of their continuous physical properties.