RESUMO
Transmission of human herpesvirus 8 (HHV-8) may occur through various routes including breastfeeding and sexual intercourse. We attempted to detect HHV-8 infection in nine HIV-positive couples discordant for Kaposi's sarcoma who maintained a monogamous sexual relationship for at least one year. By quantitative real-time polymerase chain reaction and HHV-8 genotyping we provide strong evidence for the sexual transmission of HHV-8 in this unique cohort.
Assuntos
Soropositividade para HIV/virologia , Herpesvirus Humano 8/isolamento & purificação , Sarcoma de Kaposi/virologia , Adulto , Terapia Antirretroviral de Alta Atividade , Estudos de Coortes , DNA Viral/análise , Feminino , Genótipo , Soropositividade para HIV/complicações , Soropositividade para HIV/tratamento farmacológico , Humanos , Leucócitos Mononucleares/virologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase/métodos , Sarcoma de Kaposi/complicações , Homologia de Sequência de Aminoácidos , Comportamento SexualRESUMO
Kaposi's sarcoma-associated herpesvirus (KSHV) is detected consistently in Kaposi's sarcoma (KS). Because of its dramatic sequence variation, the K1 gene has been used to classify KSHV. We found a diverse array of KSHV subtypes A1, A2, A3, A5, B1, B2, and C3 in 23 Cuban KS samples containing several novel sporadic insertions/deletions in subtypes A and C. The molecular epidemiology of the KSHV subtypes seems to reflect the unique mixed ethnic background of the Cuban population.