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1.
J Clin Immunol ; 41(4): 756-768, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33464451

RESUMO

Human nude SCID is a rare autosomal recessive inborn error of immunity (IEI) characterized by congenital athymia, alopecia, and nail dystrophy. Few cases have been reported to date. However, the recent introduction of newborn screening for IEIs and high-throughput sequencing has led to the identification of novel and atypical cases. Moreover, immunological alterations have been recently described in patients carrying heterozygous mutations. The aim of this paper is to describe the extended phenotype associated with FOXN1 homozygous, compound heterozygous, or heterozygous mutations. We collected clinical and laboratory information of a cohort of 11 homozygous, 2 compound heterozygous, and 5 heterozygous patients with recurrent severe infections. All, except one heterozygous patient, had signs of CID or SCID. Nail dystrophy and alopecia, that represent the hallmarks of the syndrome, were not always present, while almost 50% of the patients developed Omenn syndrome. One patient with hypomorphic compound heterozygous mutations had a late-onset atypical phenotype. A SCID-like phenotype was observed in 4 heterozygous patients coming from the same family. A spectrum of clinical manifestations may be associated with different mutations. The severity of the clinical phenotype likely depends on the amount of residual activity of the gene product, as previously observed for other SCID-related genes. The severity of the manifestations in this heterozygous family may suggest a mechanism of negative dominance of the specific mutation or the presence of additional mutations in noncoding regions.


Assuntos
Fatores de Transcrição Forkhead/genética , Heterozigoto , Homozigoto , Mutação , Fenótipo , Imunodeficiência Combinada Severa/diagnóstico , Imunodeficiência Combinada Severa/etiologia , Linhagem Celular , Pré-Escolar , Análise Mutacional de DNA , Gerenciamento Clínico , Feminino , Fatores de Transcrição Forkhead/química , Estudos de Associação Genética , Loci Gênicos , Predisposição Genética para Doença , Transplante de Células-Tronco Hematopoéticas , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Modelos Moleculares , Conformação Molecular , Linhagem , Imunodeficiência Combinada Severa/terapia , Relação Estrutura-Atividade , Resultado do Tratamento
2.
Environ Monit Assess ; 191(3): 128, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30721416

RESUMO

Climate and rich volcanic soils of Fogo Island (Cape Verde) are optimal conditions for grape agriculture. This study aims a first evaluation of the bioaccumulation of essential and non-essential elements in different parts of vines of the same variety (Vitis vinifera L.), grown on recent pyroclasts and lahar deposits. Chemical composition was obtained by instrumental neutron activation analysis. A general decrease of the chemical contents occurs in the following order: barks, leaves, and grapes. Lower chemical contents were found in the grapes cultivated on the lahar deposit, except for barium. Potassium and bromine are the most accumulated. A tendency for lower transfer coefficients and enrichment factors (EF) of the chemical elements studied occur in vines grown in lahar. Significant EF of the majority of the elements studied were observed, particularly in leaves and grapes. Among the rare earth elements (REE), the heavy ones are significantly enriched in grapes. Slight positive Eu anomalies occur, which can be explained as inherited from the soil, and by a preferential uptake of Eu2+, replacing Ca2+. Among potential harmful chemical elements, significant EF (> 10) for Cr, As, Sb, W, and U in the two vines occur. Although its low concentration, the results obtained point to U bioavailability. The bioaccumulation of some chemical elements in vines from Fogo Island may be due to several factors of geogenic/natural origin, namely soil composition, airborne fine particles, and the climatic conditions of aridity with a potential availability when raindrops fall.


Assuntos
Monitoramento Ambiental/métodos , Frutas/química , Casca de Planta/química , Folhas de Planta/química , Poluentes do Solo/análise , Solo/química , Vitis/química , Agricultura , Bário/análise , Bromo/análise , Cabo Verde , Ilhas , Metais Terras Raras/análise , Análise de Ativação de Nêutrons , Potássio/análise
4.
J Paediatr Child Health ; 58(9): 1697, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-34652036
5.
Blood ; 117(2): 688-96, 2011 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-20978268

RESUMO

FOXN1 deficiency is a primary immunodeficiency characterized by athymia, alopecia totalis, and nail dystrophy. Two infants with FOXN1 deficiency were transplanted with cultured postnatal thymus tissue. Subject 1 presented with disseminated Bacillus Calmette-Guérin infection and oligoclonal T cells with no naive markers. Subject 2 had respiratory failure, human herpes virus 6 infection, cytopenias, and no circulating T cells. The subjects were given thymus transplants at 14 and 9 months of life, respectively. Subject 1 received immunosuppression before and for 10 months after transplantation. With follow up of 4.9 and 2.9 years, subjects 1 and 2 are well without infectious complications. The pretransplantation mycobacterial disease in subject 1 and cytopenias in subject 2 resolved. Subject 2 developed autoimmune thyroid disease 1.6 years after transplantation. Both subjects developed functional immunity. Subjects 1 and 2 have 1053/mm(3) and 1232/mm(3) CD3(+) cells, 647/mm(3) and 868/mm(3) CD4(+) T cells, 213/mm(3) and 425/mm(3) naive CD4(+) T cells, and 10 200 and 5700 T-cell receptor rearrangement excision circles per 100 000 CD3(+) cells, respectively. They have normal CD4 T-cell receptor ß variable repertoires. Both subjects developed antigen-specific proliferative responses and have discontinued immunoglobulin replacement. In summary, thymus transplantation led to T-cell reconstitution and function in these FOXN1 deficient infants.


Assuntos
Fatores de Transcrição Forkhead/deficiência , Imunodeficiência Combinada Severa/imunologia , Imunodeficiência Combinada Severa/fisiopatologia , Imunodeficiência Combinada Severa/cirurgia , Timo/transplante , Separação Celular , Feminino , Citometria de Fluxo , Humanos , Imunofenotipagem , Lactente , Masculino
6.
PLoS One ; 7(5): e37042, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22590644

RESUMO

Forkhead box N1 (FOXN1) is a transcription factor crucial for thymic epithelium development and prevention of its involution. Investigation of a patient with a rare homozygous FOXN1 mutation (R255X), leading to alopecia universalis and thymus aplasia, unexpectedly revealed non-maternal circulating T-cells, and, strikingly, large numbers of aberrant double-negative αß T-cells (CD4negCD8neg, DN) and regulatory-like T-cells. These data raise the possibility that a thymic rudiment persisted, allowing T-cell development, albeit with disturbances in positive/negative selection, as suggested by DN and FoxP3+ cell expansions. Although regulatory-like T-cell numbers normalized following HLA-mismatched thymic transplantation, the αßDN subset persisted 5 years post-transplantation. Involution of thymus allograft likely occurred 3 years post-transplantation based on sj/ßTREC ratio, which estimates intrathymic precursor T-cell divisions and, consequently, thymic explant output. Nevertheless, functional immune-competence was sustained, providing new insights for the design of immunological reconstitution strategies based on thymic transplantation, with potential applications in other clinical settings.


Assuntos
Alopecia/genética , Alopecia/terapia , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Mutação de Sentido Incorreto , Timócitos/metabolismo , Timo/transplante , Alopecia/metabolismo , Feminino , Humanos , Lactente , Timo/metabolismo , Transplante Homólogo
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