RESUMO
Two isolates from Canada and the USA (UAMH 7122 and UAMH 7211, respectively) previously identified as Stachybotrys eucylindrosporus were studied by morphology and six-locus phylogeny (cmdA, ITS, LSU, rpb2, tef1α and tub2). UAMH 7122 and UAMH 7211 are morphologically related but phylogenetically distinct from Striatibotrys eucylindrosporus (≡Stachybotrys eucylindrosporus) and Str. rhabdosporus. Hence, UAMH 7122 and UAMH 7211 are described as a new species, Striatibotrys neoeucylindrosporus sp. nov. with UAMH 7211 as the holotype. The characters of this species include some phialides proliferating by holoblastic extension of phialides and conidia clavate, subcylindrical or cylindrical ellipsoid, or dumbbell-shaped, dark brown to olivaceous grey when mature, longitudinally striate, 10.3-12.3×3-3.8 µm. A key to the species of Striatibotrys is provided.
Assuntos
Filogenia , Stachybotrys/classificação , Canadá , DNA Fúngico/genética , Técnicas de Tipagem Micológica , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Esporos Fúngicos , Stachybotrys/isolamento & purificação , Estados UnidosRESUMO
Boxwood blight, a fungal disease of ornamental plants (Buxus spp.), is caused by two sister species, Calonectria pseudonaviculata (Cps) and C. henricotiae (Che). Compared to Cps, Che is documented to display reduced sensitivity to fungicides, including the azole class of antifungals, which block synthesis of a key fungal membrane component, ergosterol. A previous study reported an ergosterol biosynthesis gene in Cps, CYP51A, to be a pseudogene, and RNA-Seq data confirm that a functional CYP51A is expressed only in Che. The lack of additional ergosterol biosynthesis genes showing significant differential expression suggests that the functional CYP51A in Che could contribute to reduced azole sensitivity when compared to Cps. RNA-Seq and bioinformatic analyses found that following azole treatment, 55 genes in Cps, belonging to diverse pathways, displayed a significant decrease in expression. Putative xenobiotic detoxification genes overexpressed in tetraconazole-treated Che encoded predicted monooxygenase and oxidoreductase enzymes. In summary, expression of a functional CYP51A gene and overexpression of predicted xenobiotic detoxification genes appear likely to contribute to differential fungicide sensitivity in these two sister taxa.
Assuntos
Azóis/farmacologia , Buxus/efeitos dos fármacos , Buxus/genética , Buxus/microbiologia , Sistema Enzimático do Citocromo P-450/genética , Proteínas Fúngicas/genética , Fungicidas Industriais/farmacologia , Biologia Computacional/métodos , Farmacorresistência Fúngica , Ergosterol/metabolismo , Perfilação da Expressão Gênica , Genoma Fúngico , Genômica/métodos , Hypocreales/efeitos dos fármacos , Inativação Metabólica/genética , Testes de Sensibilidade Microbiana , Doenças das Plantas/microbiologia , TranscriptomaRESUMO
Functional toxicology has enabled the identification of genes involved in conferring tolerance and sensitivity to engineered nanomaterial (ENM) exposure in the model plant Arabidopsis thaliana (L.) Heynh. Several genes were found to be involved in metabolic functions, stress response, transport, protein synthesis, and DNA repair. Consequently, analysis of physiological parameters, metal content (through ICP-MS quantification), and gene expression (by RT-qPCR) of A. thaliana orthologue genes were performed across different plant species of agronomic interest to highlight putative biomarkers of exposure and effect related to ENMs. This approach led to the identification of molecular markers in Solanum lycopersicum L. and Cucurbita pepo L. (tomato and zucchini) that might not only indicate exposure to ENMs (CuO, CeO2, and La2O3) but also provide mechanistic insight into response to these materials. Through Gene Ontology (GO) analysis, the target genes were mapped in complex interatomic networks representing molecular pathways, cellular components, and biological processes involved in ENM response. The transcriptional response of 38 (out of 204) candidate genes studied varied according to particle type, size, and plant species. Importantly, some of the genes studied showed potential as biomarkers of ENM exposure and effect and may be useful for risk assessment in foods and in the environment.
Assuntos
Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Solanum lycopersicum , NanoestruturasRESUMO
The population structure of Armillaria gallica, an important pathogen of Quercus spp., was investigated from mixed oak forests in central Massachusetts, encompassing a sampling area over 500 km(2). From 16 plots at four sites a total of 153 isolates (34-40 isolates per site) was analyzed with amplified fragment length polymorphisms (AFLPs). Analyses of 204 polymorphic loci detected 38 AFLP genotypes from a sample area of 4.51 hectares (ha). Genets ranged in distribution from five to 33 genets per hectare (GPH), with a mean of eight GPH and the average A. gallica genet occupying 0.13 ha. Allele frequencies produced an unbiased expected heterozygosity (H(E)) value of 0.112 (SE = 0.006) and a Nei's expected heterozygosity (H(J)) value of 0.190 (SE = 0.009), indicating low genetic diversity within the population. Analysis of molecular variation (Φ(PT) = 0.301; P < 0.001) indicates high genetic differentiation, with 70% of the molecular variation explained at the site-level within A. gallica subpopulations. However, results of the Mantel test, used to assess the isolation-by-distance hypothesis, were inconclusive in determining whether the subpopulations were truly isolated by distance. A neighbor-joining tree constructed from a genetic distance matrix grouped genotypes from the same site (subpopulation) together, but from three of four sites genotypes were randomly clustered at the plot level. The results suggest that basidiospore dispersal is an important means of new genet formation at linear distances up to 2000 m.
Assuntos
Armillaria/genética , Micorrizas/genética , Quercus/microbiologia , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Frequência do Gene , Marcadores Genéticos , Variação Genética , Genética Populacional , Genótipo , Massachusetts , Polimorfismo Genético , Microbiologia do SoloRESUMO
Sudden vegetation dieback (SVD) is the loss of smooth cordgrass (Spartina alterniflora) along intertidal creeks in salt marshes of the Atlantic and Gulf states. The underlying cause of SVD remains unclear, but earlier work suggested a contributing role for Fusarium spp. in Louisiana. This report investigated whether these or other Fusarium species were associated with S. alterniflora dieback in mid- to north-Atlantic states. Isolations from seven SVD sites yielded 192 isolates of Fusarium spp., with more than 75% isolated from aboveground tissue. Most isolates (88%) fell into two undescribed morphospecies (MS) distinguished from each other by macroconidial shape, phialide ontogeny and growth rates. Pathogenicity tests on wound-inoculated S. alterniflora stems and seedling roots revealed that isolates in MS1 were more virulent than those in MS2 but no single isolate caused plant mortality. No matches to known species of Fusarium were revealed by DNA sequence queries of translation elongation factor 1-α (tef1) sequences. A phylogenetic analysis of partial sequences of three genes, ß-tubulin (ß-tub), calmodulin (cal) and tef1, was conducted on representative isolates from MS1 (n = 20) and MS2 (n = 18); it provided strong evidence that the MS1 isolates form a clade that represents a heretofore undescribed species, which we designate Fusarium palustre sp. nov. Isolates from the more variable MS2 clustered with the F. incarnatum-equiseti species complex as F. cf. incarnatum. Although a strong association exists between both species and declining S. alterniflora in SVD sites, neither appears to play a primary causal role in SVD. However, our findings suggest that F. palustre might play an important secondary role in the ecological disruption of the salt marshes.
Assuntos
Fusarium/classificação , Doenças das Plantas/microbiologia , Poaceae , Oceano Atlântico , Calmodulina/biossíntese , DNA Fúngico/análise , Fusarium/genética , Fusarium/ultraestrutura , Genes Fúngicos/genética , Filogenia , Raízes de Plantas/crescimento & desenvolvimento , Caules de Planta/crescimento & desenvolvimento , Análise de Sequência de DNA/métodos , Esporos Fúngicos/citologia , Esporos Fúngicos/crescimento & desenvolvimento , Tubulina (Proteína)/biossíntese , Áreas AlagadasRESUMO
Calonectria pseudonaviculata and C. henricotiae are two closely related fungal species responsible for boxwood blight disease of ornamental shrubs (Buxus spp.) in the U.S. and Europe. A previous study has shown isolates of the latter species, which is restricted to Europe, to be less sensitive to tetraconazole, an azole fungicide. In this study, we have analyzed the CYP51 paralogs for polymorphism in 26 genomes, representing geographically disparate populations of C. pseudonaviculata (n = 19) and C. henricotiae (n = 7), from the U.S., Europe, Asia, and New Zealand. The presence of a CYP51A pseudogene and lack of a functional CYP51A paralog in all C. pseudonaviculata genomes examined is a novel discovery for fungi and could have implications for the evolution of resistance to antifungal chemicals.
RESUMO
The study of quantitative traits provides a window on the interactions between multiple unlinked genetic loci. The interaction between hosts and pathogenic microbes, such as fungi, involves aspects of quantitative genetics for both partners in this dynamic equilibrium. One important pathogenic fungus is Cryptococcus neoformans, a basidiomycete yeast that can infect the human brain and whose mating system has two mating type alleles, a and alpha. The alpha mating-type allele has previously been linked to increased virulence potential. Here congenic C. neoformans strains were generated in the two well-characterized genetic backgrounds B3501alpha and NIH433a to examine the potential influence of genes outside of the mating-type locus on the virulence potential of mating type. The congenic nature of these new strain pairs was established by karyotyping, amplified fragment length polymorphism genotyping, and whole-genome molecular allele mapping (congenicity mapping). Virulence studies revealed that virulence was equivalent between the B3501 a and alpha congenic strains but the alpha strain was more virulent than its a counterpart in the NIH433 genetic background. These results demonstrate that genomic regions outside the mating type locus contribute to differences in virulence between a and alpha cells. The congenic strains described here provide a foundation upon which to elucidate at genetic and molecular levels how mating-type and other unlinked loci interact to enable microbial pathogenesis.
Assuntos
Cryptococcus neoformans/genética , Cryptococcus neoformans/patogenicidade , Genes Fúngicos Tipo Acasalamento , Animais , Mapeamento Cromossômico , Feminino , Marcadores Genéticos , Camundongos , Camundongos Endogâmicos DBA , VirulênciaRESUMO
Rapid and accurate molecular diagnostic tools are critical to efforts to minimize the impact and spread of emergent pathogens. The identification of diagnostic markers for novel pathogens presents several challenges, especially in the absence of information about population diversity and where genetic resources are limited. The objective of this study was to use comparative genomics datasets to find unique target regions suitable for the diagnosis of two fungal species causing a newly emergent blight disease of boxwood. Candidate marker regions for loop-mediated isothermal amplification (LAMP) assays were identified from draft genomes of Calonectria henricotiae and C. pseudonaviculata, as well as three related species not associated with this disease. To increase the probability of identifying unique targets, we used three approaches to mine genome datasets, based on (i) unique regions, (ii) polymorphisms, and (iii) presence/absence of regions across datasets. From a pool of candidate markers, we demonstrate LAMP assay specificity by testing related fungal species, common boxwood pathogens, and environmental samples containing 445 diverse fungal taxa. This comparative-genomics-based approach to the development of LAMP diagnostic assays is the first of its kind for fungi and could be easily applied to diagnostic marker development for other newly emergent plant pathogens.
Assuntos
Ascomicetos/isolamento & purificação , Buxus/microbiologia , Técnicas de Amplificação de Ácido Nucleico/métodos , Doenças das Plantas/microbiologia , Ascomicetos/classificação , Ascomicetos/genética , Biologia Computacional , Genômica , Sensibilidade e EspecificidadeRESUMO
To construct a genetic linkage map of the heterothallic yeast, Cryptococcus neoformans (Filobasidiella neoformans), we crossed two mating-compatible strains and analyzed 94 progeny for the segregation of 301 polymorphic markers, consisting of 228 restriction site polymorphisms, 63 microsatellites, two indels, and eight mating-type (MAT)-associated markers. All but six markers showed no significant (P < 0.05) segregation distortion. At a minimum LOD score of 6.0 and a maximum recombination frequency of 0.30, 20 linkage groups were resolved, resulting in a map length of approximately 1500 cM. Average marker density is 5.4 cM (range 1-28.7 cM). Hybridization of selected markers to blots of electrophoretic karyotypes unambiguously assigned all linkage groups to chromosomes and led us to conclude that the C. neoformans genome is approximately 20.2 Mb, comprising 14 chromosomes ranging in size from 0.8 to 2.3 Mb, with a ratio of approximately 13.2 kb/cM averaged across the genome. However, only 2 of 12 ungrouped markers hybridized to chromosome 10. The hybridizations revealed at least one possible reciprocal translocation involving chromosomes 8, 9, and 12. This map has been critical to genome sequence assembly and will be essential for future studies of quantitative trait inheritance.
Assuntos
Mapeamento Cromossômico , Cryptococcus neoformans/classificação , Cryptococcus neoformans/genética , Cromossomos Fúngicos/genética , Marcadores Genéticos , Cariotipagem , Escore Lod , Polimorfismo Genético , Mapeamento por RestriçãoRESUMO
Neonectria ditissima is a fungal pathogen native to eastern North America that causes disfiguring cankers on numerous tree species, particularly birches (Betula spp.). In order to develop control strategies, fundamental knowledge of the pathogen's reproductive and dispersal dynamics is necessary. To undertake these studies, we developed genomic libraries enriched for clones containing microsatellites, and then designed primers flanking each locus. Of 34 loci that were screened, 11 were polymorphic among 38 isolates obtained from a single population. Gene diversities ranged from 0.05 to 0.862 with a mean of 0.409. These markers will be invaluable in population studies of this fungus.
RESUMO
Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains approximately 6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.
Assuntos
Cryptococcus neoformans/genética , Genoma Fúngico , Processamento Alternativo , Parede Celular/metabolismo , Cromossomos Fúngicos/genética , Biologia Computacional , Cryptococcus neoformans/patogenicidade , Cryptococcus neoformans/fisiologia , Elementos de DNA Transponíveis , Proteínas Fúngicas/metabolismo , Biblioteca Gênica , Genes Fúngicos , Humanos , Íntrons , Dados de Sequência Molecular , Fenótipo , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Polissacarídeos/metabolismo , RNA Antissenso , Análise de Sequência de DNA , Transcrição Gênica , Virulência , Fatores de Virulência/metabolismoRESUMO
Selfing in the chestnut blight fungus, Cryphonectria parasitica, occurs by two different genetic mechanisms. Most self-fertile isolates of C. parasitica are heterokaryotic for mating type, and the progeny from selfing segregate for mating type. Further, we resolved mating-type (MAT) heterokaryons into homokaryons of both mating types by isolating uninucleate asexual spores (conidia). However, because ascospore progeny, with rare exceptions, are not MAT heterokaryons, C. parasitica must lack a regular mechanism to maintain heterokaryosis by selfing. We hypothesize that heterokaryon formation may occur either because of recurrent biparental inbreeding, or by mating-type switching, possibly one involving some kind of parasexual process. The second mechanism found for selfing in C. parasitica occurred less frequently. Three single-conidial isolates (MAT-1 and MAT-2) selfed and produced progeny that did not segregate for mating type. It is currently not known if meiosis occurs during ascospore formation by this mechanism.
Assuntos
Ascomicetos/genética , Ascomicetos/fisiologia , Genes Fúngicos , Genes Fúngicos Tipo Acasalamento , Alelos , Núcleo Celular/genética , Cruzamentos Genéticos , DNA Fúngico/análise , Genótipo , Reação em Cadeia da Polimerase , Esporos Fúngicos/genéticaRESUMO
The most common cause of fungal meningitis in humans, Cryptococcus neoformans serotype A, is a basidiomycetous yeast with a bipolar mating system. However, the vast majority (>99.9%) of C. neoformans serotype A isolates possess only one of the two mating type alleles (MATalpha). Isolates with the other allele (MATa) were recently discovered and proven to mate in the laboratory. It has been a mystery whether and where C. neoformans strains undergo sexual reproduction. Here, we applied population genetic approaches to demonstrate that a population of C. neoformans serotype A clinical isolates from Botswana contains an unprecedented proportion of fertile MATa isolates and exhibits evidence of both clonal expansion and recombination within two partially genetically isolated subgroups. Our findings provide evidence for sexual recombination among some populations of C. neoformans serotype A from sub-Saharan Africa, which may have a direct impact on their evolution.