Platinum Nanozyme-Enabled Colorimetric Determination of Total Antioxidant Level in Saliva.

Redox imbalance and oxidative stress-related biomarkers are raising increasing consensus in the scientific community for their significant role in a wide range of human disorders. In this framework, the total antioxidant capacity (TAC), namely, the overall pattern of both enzymatic and nonenzymatic antioxidant compounds within the body, represents an important bioanalytical parameter. To date, however, antioxidant assays require costly instrumentations, laboratory setups, and reagents, and they are invasive. Yet, their accuracy typically suffers from strong sensitivity to interfering matrices and inability to detect the complete pattern of physiological antioxidant molecules, due to the use of reaction schemes and probes/substrates that are not sensitive to the diverse range of relevant target species. Here, we exploit the enzyme-mimetic properties of platinum nanoparticles combined with hydroxyl radical probes produced at the particle surface to develop an effective detection scheme that is sensitive to both single electron transfer (SET) and hydrogen atom transfer (HAT) reactions, thus covering all the physiologically relevant antioxidant species. Importantly, the nanozyme-enabled method allows fast (5 min), accurate, and noninvasive evaluation of the body TAC through saliva via simple naked-eye or smartphone-based inspection.

Folding-Based Electrochemical Aptasensor for the Determination of ß-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes.

Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of ß-lactoglobulin (ß-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-ß-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains ß-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of ß-LG within the range 0.1-10 ng·mL-1, with a limit of detection of 0.09 ng·mL-1. The biosensor was satisfactorily employed for the determination of spiked ß-LG in real food samples.

NanoMIP-based approach for the suppression of interference signals in electrochemical sensors.

Herein, we describe the use of molecularly imprinted nanoparticles (nanoMIPs) as sequestering (masking) agents, to suppress the signal coming from interfering molecules and facilitate the detection of the target analyte. In this work, ascorbic acid was used as a model interfering molecule in dopamine electrochemical detection. NanoMIPs selective for ascorbic acid demonstrated to be capable of binding and suppressing electrochemical signal from ascorbic acid, enabling the detection of dopamine in the range 100-500 nM, without any need for sample pre-treatment. Tests in real samples (spiked human serum) were also carried out successfully. Due to the generic nature of the imprinting process, the proposed approach can be tailored to suppress potentially any interfering species, by simply varying the type of nanoMIPs used.

Electrochemical Nanocomposite Single-Use Sensor for Dopamine Detection.

In this work, we report the development of a simple and sensitive sensor based on graphite screen-printed electrodes (GSPEs) modified by a nanocomposite film for dopamine (DA) detection. The sensor was realized by electrodepositing polyaniline (PANI) and gold nanoparticles (AuNPs) onto the graphite working electrode. The sensor surface was fully characterized by means of the cyclic voltammetry (CV) technique using [Fe(CN)6]4-/3- and [Ru(NH3)6]2+/3+ as redox probes. The electrochemical behavior of the nanocomposite sensor towards DA oxidation was assessed by differential pulse voltammetry (DPV) in phosphate buffer saline at physiological pH. The sensor response was found to be linearly related to DA concentration in the range 1-100 µM DA, with a limit of detection of 0.86 µM. The performance of the sensor in terms of reproducibility and selectivity was also studied. Finally, the sensor was successfully applied for a preliminary DA determination in human serum samples.

Nano-Biosensing Platforms for Detection of Cow's Milk Allergens: An Overview.

Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins' presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

Electrochemical Fingerprint of Arsenic (III) by Using Hybrid Nanocomposite-Based Platforms.

Arsenic, one of the most abundant mineral and also one to the most toxic compounds. Due to its high toxicity sensitive analytical methods are highly important, taking into account that the admitted level is in the range of µg L-1. A novel and easy to use platform for As(III) detection from water samples is proposed, based on gold and platinum bi metallic nanoparticles and a conductive polymer (polyaniline). The electrochemical detection was achieved after optimization of cathodic pre-concentration and stripping parameters by square wave anodic stripping voltammetry at modified screen-printed carbon-based electrochemical cells, proving its applicability for disposable and cost-effective in situ analysis of arsenic.

Point-of-Care Strategies for Detection of Waterborne Pathogens.

Waterborne diseases that originated due to pathogen microorganisms are emerging as a serious global health concern. Therefore, rapid, accurate, and specific detection of these microorganisms (i.e., bacteria, viruses, protozoa, and parasitic pathogens) in water resources has become a requirement of water quality assessment. Significant research has been conducted to develop rapid, efficient, scalable, and affordable sensing techniques to detect biological contaminants. State-of-the-art technology-assisted smart sensors have improved features (high sensitivity and very low detection limit) and can perform in a real-time manner. However, there is still a need to promote this area of research, keeping global aspects and demand in mind. Keeping this view, this article was designed carefully and critically to explore sensing technologies developed for the detection of biological contaminants. Advancements using paper-based assays, microfluidic platforms, and lateral flow devices are discussed in this report. The emerging recent trends, mainly point-of-care (POC) technologies, of water safety analysis are also discussed here, along with challenges and future prospective applications of these smart sensing technologies for water health diagnostics.

Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review.

Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010-2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.

DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos.

In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM.

MIP-Based Sensors: Promising New Tools for Cancer Biomarker Determination.

Detecting cancer disease at an early stage is one of the most important issues for increasing the survival rate of patients. Cancer biomarker detection helps to provide a diagnosis before the disease becomes incurable in later stages. Biomarkers can also be used to evaluate the progression of therapies and surgery treatments. In recent years, molecularly imprinted polymer (MIP) based sensors have been intensely investigated as promising analytical devices in several fields, including clinical analysis, offering desired portability, fast response, specificity, and low cost. The aim of this review is to provide readers with an overview on recent important achievements in MIP-based sensors coupled to various transducers (e.g., electrochemical, optical, and piezoelectric) for the determination of cancer biomarkers by selected publications from 2012 to 2016.

Gold and Magnetic Nanoparticles-Based Electrochemical Biosensors for Cancer Biomarker Determination.

Detecting cancer at early stage is one of the most important factors associated with the increase of the survival rate of the patients. Cancer biomarkers are able to detect a specific disease early and help to provide treatments before it becomes incurable in later stages. Biomarkers can also be used to determine the recurrence of the disease and to evaluate the follow-up of the patients after a chemio- or radio-therapy and surgery treatments. Electrochemical biosensors are successfully applied for the detection of cancer biomarkers due to their high sensibility, rapid response and low cost. In recent years, the advance in nanotechnology has led to the discovery and the employment of a great number of new materials in nanoscale dimensions. Due to their particular properties, the development of nanostructured biosensors (in particular using gold and magnetic nanoparticles) with high analytical performances increases constantly. In this review recent different strategies for the development of gold and magnetic nanoparticles-based electrochemical biosensors for cancer biomarkers detection were presented.

In vitro assessment of antibody-conjugated gold nanorods for systemic injections.

BACKGROUND: The interest for gold nanorods in biomedical optics is driven by their intense absorbance of near infrared light, their biocompatibility and their potential to reach tumors after systemic administration. Examples of applications include the photoacoustic imaging and the photothermal ablation of cancer. In spite of great current efforts, the selective delivery of gold nanorods to tumors through the bloodstream remains a formidable challenge. Their bio-conjugation with targeting units, and in particular with antibodies, is perceived as a hopeful solution, but the complexity of living organisms complicates the identification of possible obstacles along the way to tumors. RESULTS: Here, we present a new model of gold nanorods conjugated with anti-cancer antigen 125 (CA125) antibodies, which exhibit high specificity for ovarian cancer cells. We implement a battery of tests in vitro, in order to simulate major nuisances and predict the feasibility of these particles for intravenous injections. We show that parameters like the competition of free CA125 in the bloodstream, which could saturate the probe before arriving at the tumors, the matrix effect and the interference with erythrocytes and phagocytes are uncritical. CONCLUSIONS: Although some deterioration is detectable, anti-CA125-conjugated gold nanorods retain their functional features after interaction with blood tissue and so represent a powerful candidate to hit ovarian cancer cells.

Synthesis of dual models multivalent activatable aptamers based on HCR and RCA for ultrasensitive detection of Salmonella typhimurium.

Aptamers have superior structural properties and have been widely used in bacterial detection methods. However, the problem of low affinity still exists in complex sample detection. In contrast, hybridization chain reaction (HCR)-based model I and rolling circle amplification (RCA)-based model II multivalent activatable aptamers (multi-Apts) can fulfill the need for low-cost, rapid, highly sensitive and high affinity detection of S. typhimurium. In our research, two models of multi-Apts were designed. First, a monovalent activatable aptamer (mono-Apt) was constructed by fluorescence resonance energy transfer (FRET) with an S. typhimurium aptamer and its complementary chain of BHQ1. Next, the DNA scaffold was obtained by HCR and RCA, and the multi-Apts were obtained by self-assembly of the mono-Apt with a DNA scaffold. In model I, when target was presented, the complementary chain BHQ1 was released due to the binding of multi-Apts to the target and was subsequently adsorbed by UIO66. Finally, a FRET-based fluorescence detection signal was obtained. In mode II, the multi-Apts bound to the target, and the complementary chain BHQ1 was released to become the trigger chain for the next round of amplification of HCR with a fluorescence detection signal. HCR and RCA based multi-Apts were able to detect S. typhimurium as low as 2 CFU mL-1 and 1 CFU mL-1 respectively. Multi-Apts amplification strategy provides a new method for early diagnosis of pathogenic microorganisms in foods.

Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay.

MicroRNAs (miRNAs, miRs) are naturally occurring small RNAs (approximately 22 nucleotides in length) that have critical functions in a variety of biological processes, including tumorigenesis. They are an important target for detection technology for future medical diagnostics. In this paper we report an electrochemical method for miRNA detection based on paramagnetic beads and enzyme amplification. In particular, miR 222 was chosen as model sequence, because of its involvement in brain, lung, and liver cancers. The proposed bioassay is based on biotinylated DNA capture probes immobilized on streptavidin-coated paramagnetic beads. Total RNA was extracted from the cell sample, enriched for small RNA, biotinylated, and then hybridized with the capture probe on the beads. The beads were then incubated with streptavidin-alkaline phosphatase and exposed to the appropriate enzymatic substrate. The product of the enzymatic reaction was electrochemically monitored. The assay was finally tested with a compact microfluidic device which enables multiplexed analysis of eight different samples with a detection limit of 7 pmol L(-1) and RSD = 15 %. RNA samples from non-small-cell lung cancer and glioblastoma cell lines were also analyzed.

Biosensors in 2022.

Sixty years have passed since Clark and Lyons proposed the concept of using glucose enzyme electrodes to monitor the oxygen that is consumed during an enzyme-catalyzed reaction [...].

On spot detection of nickel and cobalt from exhausted batteries by a smart electrochemical sensor.

This work presents the realization and the application of an user-friendly electrochemical platform based on screen-printed electrodes for the simultaneous determination of nickel and cobalt ions in real samples by means of square wave adsorptive stripping voltammetry (SWAdSV). The sensor was realized by electrodepositing in situ a bismuth film onto graphite screen-printed electrodes (GSPEs). The sensor surface was fully characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The experimental conditions for the determination of nickel and cobalt in the form of dimethylglyoximate complexes were studied and optimized. Linear calibration curves for Ni(II) and Co(II), determined individually and together, in the range 10-40 µg/L for nickel and 10-60 µg/L for cobalt, respectively, were obtained. The limits of detection for nickel and cobalt determination were 2.5 µg/L and 2.4 µg/L, respectively. The performance of the sensor in terms of reproducibility and selectivity was also studied. The applicability of the developed platform was assessed by determining nickel and cobalt in samples deriving from an industrial process of recycling exhausted batteries and in soil samples.

Ara H1 peanut allergen detection using a labelled electrochemical aptasensor based on GO-COOH@bimetallic composite platform.

Allergies are defined as a hypersensitivity reaction, immunologically mediated, as a result to an external stimulus. Peanuts induced allergies are considered one of the most severe, life-threatening food sensitivities since they trigger the highest frequency of severe and fatal reactions, even in trace amounts. Therefore, it is imperative to develop fast, accurate and easy-to-use analytical methods to determine Ara h1, is a seed storage protein from Arachis hypogea and the main peanut derived allergen. In this work, two strategies were applied to develop an electrochemical aptasensor based on GO-COOH and metallic nanoparticles immobilised on screen-printed carbon electrodes (SPCEs). The analytical performances of the aptasensor showed a linear range of 5-150 nM, and a limit of detection of 1.66 nM. The method was applied in peanut-free food samples with very good recoveries proving to be a promising tool for peanut allergy prevention.

Trends and prospects of 2-D tungsten disulphide (WS2) hybrid nanosystems for environmental and biomedical applications.

Recently, 2D layered transition metal dichalcogenides (TMDCs) with their ultrathin sheet nanostructure and diversified electronic structure have drawn attention for various advanced applications to achieve high-performance parameters. Unique 2D TMDCs mainly comprise transition metal and chalcogen element where chalcogen element layers sandwich the transition metal element layer. In such a case, various properties can be enhanced and controlled depending on the targeted application. Among manipulative 2D TMDCs, tungsten disulphide (WS2) is one of the emerging nano-system due to its fascinating properties in terms of direct band gap, higher mobility, strong photoluminescence, good thermal stability, and strong magnetic field interaction. The advancement in characterization techniques, especially scattering techniques, can help in study of opto-electronic properties of 2D TMDCs along with determination of layer variations and investigation of defect. In this review, the fabrication and applications are well summarized to optimize an appropriate WS2-TMDCs assembly according to focused field of research. Here, the scientific investigations on 2D WS2 are studied in terms of its structure, role of scattering techniques to study its properties, and synthesis routes followed by its potential applications for environmental remediation (e.g., photocatalytic degradation of pollutants, gas sensing, and wastewater treatment) and biomedical domain (e.g., drug delivery, photothermal therapy, biomedical imaging, and biosensing). Further, a special emphasis is given to the significance of 2D WS2 as a substrate for surface-enhanced Raman scattering (SERS). The discussion is further extended to commercial and industrial aspects, keeping in view major research gaps in existing research studies.

A novel electrochemical aptasensor based on core-shell nanomaterial labeling for simultaneous detection of acetamiprid and malathion.

At present, due to the coexistence of multiple pesticides in vegetables and the enhanced toxicity, a simultaneous detection method for multiple pesticides is urgently needed. In this work, two types of core-shell nanomaterials, Ag-Au core-shell nanoparticles (Ag@Au NPs) and Cu2O-Au core-shell nanoparticles (Cu2O@Au NPs), were synthesized and labeled with acetamiprid aptamer and malathion aptamer to prepare two novel electroactive signal probes, respectively. The two probes were hybridized on the surface of the electrode by the principle of base complementary pairing between the aptamers and the thiolated DNA oligonucleotide sequences, and a dual-signal electrochemical aptasensor for the simultaneous detection of acetamiprid and malathion was established by modified glassy carbon electrode (GCE). The limits of detection (LOD) were calculated to be 43.7 pg mL-1 for acetamiprid and 63.4 pg mL-1 for malathion. The aptasensor determined acetamiprid and malathion in spinach and rape with the recovery rates of 88.9%-112.5% and 98.0%-114.1%, respectively.

Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses.

Recent years have witnessed the emergence of several viruses and other pathogens. Some of these infectious diseases have spread globally, resulting in pandemics. Although biosensors of various types have been utilized for virus detection, their limited sensitivity remains an issue. Therefore, the development of better diagnostic tools that facilitate the more efficient detection of viruses and other pathogens has become important. Nanotechnology has been recognized as a powerful tool for the detection of viruses, and it is expected to change the landscape of virus detection and analysis. Recently, nanomaterials have gained enormous attention for their value in improving biosensor performance owing to their high surface-to-volume ratio and quantum size effects. This article reviews the impact of nanotechnology on the design, development, and performance of sensors for the detection of viruses. Special attention has been paid to nanoscale materials, various types of nanobiosensors, the internet of medical things, and artificial intelligence-based viral diagnostic techniques.