RESUMO
RATIONALE: Cardiac myocyte contraction is caused by Ca(2+) binding to troponin C, which triggers the cross-bridge power stroke and myofilament sliding in sarcomeres. Synchronized Ca(2+) release causes whole cell contraction and is readily observable with current microscopy techniques. However, it is unknown whether localized Ca(2+) release, such as Ca(2+) sparks and waves, can cause local sarcomere contraction. Contemporary imaging methods fall short of measuring microdomain Ca(2+)-contraction coupling in live cardiac myocytes. OBJECTIVE: To develop a method for imaging sarcomere level Ca(2+)-contraction coupling in healthy and disease model cardiac myocytes. METHODS AND RESULTS: Freshly isolated cardiac myocytes were loaded with the Ca(2+)-indicator fluo-4. A confocal microscope equipped with a femtosecond-pulsed near-infrared laser was used to simultaneously excite second harmonic generation from A-bands of myofibrils and 2-photon fluorescence from fluo-4. Ca(2+) signals and sarcomere strain correlated in space and time with short delays. Furthermore, Ca(2+) sparks and waves caused contractions in subcellular microdomains, revealing a previously underappreciated role for these events in generating subcellular strain during diastole. Ca(2+) activity and sarcomere strain were also imaged in paced cardiac myocytes under mechanical load, revealing spontaneous Ca(2+) waves and correlated local contraction in pressure-overload-induced cardiomyopathy. CONCLUSIONS: Multimodal second harmonic generation 2-photon fluorescence microscopy enables the simultaneous observation of Ca(2+) release and mechanical strain at the subsarcomere level in living cardiac myocytes. The method benefits from the label-free nature of second harmonic generation, which allows A-bands to be imaged independently of T-tubule morphology and simultaneously with Ca(2+) indicators. Second harmonic generation 2-photon fluorescence imaging is widely applicable to the study of Ca(2+)-contraction coupling and mechanochemotransduction in both health and disease.
Assuntos
Cardiomiopatias/metabolismo , Acoplamento Excitação-Contração , Microdomínios da Membrana/metabolismo , Microscopia Confocal , Microscopia de Fluorescência por Excitação Multifotônica , Imagem Multimodal/métodos , Contração Miocárdica , Miócitos Cardíacos/metabolismo , Sarcômeros/metabolismo , Compostos de Anilina , Animais , Cardiomiopatias/fisiopatologia , Modelos Animais de Doenças , Corantes Fluorescentes , Cinética , Masculino , Mecanotransdução Celular , Camundongos , Ratos Sprague-Dawley , Estresse Mecânico , XantenosRESUMO
We report an accurate method to count red blood cells, platelets, and white blood cells, as well as to determine hemoglobin in the blood of humans, horses, dogs, cats, and cows. Red and white blood cell counts can also be performed on human body fluids such as cerebrospinal fluid, synovial fluid, and peritoneal fluid. The approach consists of using a compact, custom-built microscope to record large field-of-view, bright-field, and fluorescence images of samples that are stained with a single dye and using automatic algorithms to count blood cells and detect hemoglobin. The total process takes about 15 min, including 5 min for sample preparation, and 10 min for data collection and analysis. The minimum volume of blood needed for the test is 0.5 µL, which allows for minimally invasive sample collection such as using a finger prick rather than a venous draw. Blood counts were compared to gold-standard automated clinical instruments, with excellent agreement between the two methods as determined by a Bland-Altman analysis. Accuracy of counts on body fluids was consistent with hand counting by a trained clinical lab scientist, where our instrument demonstrated an approximately 100-fold lower limit of detection compared to current automated methods. The combination of a compact, custom-built instrument, simple sample collection and preparation, and automated analysis demonstrates that this approach could benefit global health through use in low-resource settings where central hematology laboratories are not accessible.
Assuntos
Contagem de Células Sanguíneas , Líquidos Corporais/citologia , Citometria de Fluxo , Animais , Gatos , Bovinos , Cães , Citometria de Fluxo/instrumentação , Voluntários Saudáveis , Cavalos , HumanosRESUMO
The autofluorescence under ultraviolet excitation arising from normal squamous and columnar esophageal mucosa is investigated using multispectral microscopy. The results suggest that the autofluorescence signal arises from the superficial tissue layer due to the short penetration depth of the ultraviolet excitation. As a result, visualization of esophageal epithelial cells and their organization can be attained using wide-field autofluorescence microscopy. Our results show tryptophan to be the dominant source of emission under 266 nm excitation, while emission from NADH and collagen are dominant under 355 nm excitation. The analysis of multispectral microscopy images reveals that tryptophan offers the highest image contrast due to its non-uniform distribution in the sub-cellular matrix. This technique can simultaneously provide functional and structural imaging of the microstructure using only the intrinsic tissue fluorophores.
Assuntos
Epitélio/patologia , Esôfago/patologia , Microscopia de Fluorescência/métodos , Óptica e Fotônica , Esôfago de Barrett/diagnóstico , Esôfago de Barrett/patologia , Biópsia , Colágeno/química , Fluorescência , Humanos , Processamento de Imagem Assistida por Computador/métodos , Mucosa/patologia , NAD/química , Triptofano/química , Raios UltravioletaRESUMO
The potential of NADH autofluorescence as an in vivo intrinsic optical signature to monitor tissue metabolism is well recognized and supported by experimental results mainly in animal models. In this work, we propose a non-contact implementation of this method using large area excitation and employing a normalization method to account for non-metabolic signal changes. Proof of principle in vivo experiments were carried out using an autofluorescence imaging experimental system and a rat renal ischemia model. A hand-held fiber-optic probe was utilized to test the ability of the signal normalization method to address operational conditions associated with the translation of this method to a clinical setting. Preliminary pre-clinical in vivo test of the probe system was carried out using the same rat model.
Assuntos
Isquemia/complicações , Rim/irrigação sanguínea , Rim/patologia , Fenômenos Ópticos , Traumatismo por Reperfusão/complicações , Análise Espectral/instrumentação , Análise Espectral/métodos , Análise de Variância , Animais , Fluorescência , Masculino , Fibras Ópticas , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Sus scrofa , Fatores de TempoRESUMO
Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation.
Assuntos
Epitélio/patologia , Doenças do Esôfago/diagnóstico , Esôfago/patologia , Microscopia de Fluorescência/métodos , Microscopia Ultravioleta/métodos , Biópsia , Progressão da Doença , Desenho de Equipamento , Doenças do Esôfago/patologia , Neoplasias Esofágicas/diagnóstico , Neoplasias Esofágicas/patologia , Gastroenterologia/instrumentação , Gastroenterologia/métodos , Humanos , Mucosa/patologia , Óptica e Fotônica , FótonsRESUMO
The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to rat kidney autofluorescence of the capsule versus cortex under ultraviolet excitation is determined from experimental results obtained using autofluorescence microscopy and a suitable mathematical model. The results allow for a quantitative assessment of the relative contribution of the signal originating in the metabolically active cortex as a function of capsule thickness for different wavelengths.
Assuntos
Cápsula Glomerular/metabolismo , Córtex Renal/metabolismo , NAD/análise , Espectrometria de Fluorescência/métodos , Algoritmos , Animais , Técnicas In Vitro , Ratos , Ratos Endogâmicos Lew , Ratos Endogâmicos WF , Ratos Sprague-Dawley , Distribuição Tecidual , Raios UltravioletaRESUMO
We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.
Assuntos
Diagnóstico por Computador/métodos , Rim/irrigação sanguínea , Rim/metabolismo , NAD/análise , Traumatismo por Reperfusão/diagnóstico , Traumatismo por Reperfusão/metabolismo , Espectrometria de Fluorescência/métodos , Animais , Biomarcadores/análise , Modelos Animais de Doenças , Masculino , Ratos , Ratos Endogâmicos WF , Sensibilidade e EspecificidadeRESUMO
BACKGROUND: Vascular stents are small tubular scaffolds used in the treatment of arterial stenosis (narrowing of the vessel). Most vascular stents are metallic and are deployed either by balloon expansion or by self-expansion. A shape memory polymer (SMP) stent may enhance flexibility, compliance, and drug elution compared to its current metallic counterparts. The purpose of this study was to describe the fabrication of a laser-activated SMP stent and demonstrate photothermal expansion of the stent in an in vitro artery model. METHODS: A novel SMP stent was fabricated from thermoplastic polyurethane. A solid SMP tube formed by dip coating a stainless steel pin was laser-etched to create the mesh pattern of the finished stent. The stent was crimped over a fiber-optic cylindrical light diffuser coupled to an infrared diode laser. Photothermal actuation of the stent was performed in a water-filled mock artery. RESULTS: At a physiological flow rate, the stent did not fully expand at the maximum laser power (8.6 W) due to convective cooling. However, under zero flow, simulating the technique of endovascular flow occlusion, complete laser actuation was achieved in the mock artery at a laser power of ~8 W. CONCLUSION: We have shown the design and fabrication of an SMP stent and a means of light delivery for photothermal actuation. Though further studies are required to optimize the device and assess thermal tissue damage, photothermal actuation of the SMP stent was demonstrated.
Assuntos
Prótese Vascular , Materiais Revestidos Biocompatíveis/química , Lasers , Polímeros/química , Stents , Desenho de Equipamento , Análise de Falha de Equipamento , Conformação Molecular , Aço Inoxidável/químicaRESUMO
Current flow-based blood counting devices require significant medical infrastructure and are not appropriate for field use. In this article we report on the development of a sample preparation, measurement, and analysis method that permits automated and accurate counting of red blood cells (RBCs), white blood cells (WBCs), and platelets, as well as allowing a 3-part differential of the WBCs to be performed on extremely small volumes of whole blood. This method is compatible with portable instrumentation that can be deployed in the field. The method consists of serially diluting blood samples first with sodium dodecyl sulfate dissolved in phosphate buffered saline, then in acridine orange dissolved in phosphate buffered saline, followed by fluorescence and dark field imaging with low magnification objectives. Image analysis is performed to extract cell counts and differentials. We performed a paired analysis of 20 volunteers with complete blood count values both within and beyond the normal reference range using a commercial automated hematology analyzer and the image-based method, with the new method achieving accuracies comparable to that of the commercial system. Because the sample preparation and imaging are simple and inexpensive to implement, this method has applications for pediatrics, clinician offices, and global health in regions that do not have access to central hematology laboratories.
Assuntos
Contagem de Células Sanguíneas/instrumentação , Contagem de Células Sanguíneas/métodos , Processamento de Imagem Assistida por Computador/métodos , Laranja de Acridina/química , Desenho de Equipamento , Humanos , Microscopia de Fluorescência , Dodecilsulfato de Sódio/químicaRESUMO
Laser tweezers Raman spectroscopy was used to characterize the oxygenation response of single normal adult, sickle, and cord blood red blood cells (RBCs) to an applied mechanical force. Individual cells were subjected to different forces by varying the laser power of a single-beam optical trap, and the intensities of several oxygenation-specific Raman spectral peaks were monitored to determine the oxygenation state of the cells. For all three cell types, an increase in laser power (or mechanical force) induced a greater deoxygenation of the cell. However, sickle RBCs deoxygenated more readily than normal RBCs when subjected to the same optical forces. Conversely, cord blood RBCs were able to maintain their oxygenation better than normal RBCs. These results suggest that differences in the chemical or mechanical properties of fetal, normal, and sickle cells affect the degree to which applied mechanical forces can deoxygenate the cell. Populations of normal, sickle, and cord RBCs were identified and discriminated based on this mechanochemical phenomenon. This study demonstrates the potential application of laser tweezers Raman spectroscopy as a single-cell, label-free analytical tool to characterize the functional (e.g., mechanical deformability, oxygen binding) properties of normal and diseased RBCs.
Assuntos
Anemia Falciforme/sangue , Deformação Eritrocítica , Eritrócitos/química , Pinças Ópticas , Análise de Célula Única/instrumentação , Análise Espectral Raman , Adulto , Desenho de Equipamento , Eritrócitos/efeitos dos fármacos , Eritrócitos Anormais/química , Eritrócitos Anormais/efeitos dos fármacos , Sangue Fetal/citologia , Hemoglobinas/análise , Humanos , Recém-Nascido , Oxigênio/farmacologia , Análise de Célula Única/métodosRESUMO
The diagnostic potential of autofluorescence (AF) microscopy under ultraviolet (UV) excitation is explored using ex vivo human specimens. The aim is to establish optical patterns (the rules for interpretation) that correspond to normal and abnormal histologies of the esophagus, spanning from early benign modifications (Barrett's esophagus) to subsequent dysplastic change and progression toward carcinoma. This was achieved by developing an image library categorized by disease progression. We considered morphological changes of disease as they are compared with histological diagnosis of the pathological specimen, as well as control samples of normal esophagus, proximal stomach, and small intestine tissue. Our experimental results indicate that UV AF microscopy could provide real-time histological information for visualizing changes in tissue microstructure that are currently undetectable using conventional endoscopic methods.
Assuntos
Esôfago de Barrett/diagnóstico , Neoplasias Esofágicas/diagnóstico , Microscopia de Fluorescência/métodos , Lesões Pré-Cancerosas/diagnóstico , Adenocarcinoma/diagnóstico , Adenocarcinoma/patologia , Esôfago de Barrett/patologia , Neoplasias Esofágicas/patologia , Esôfago/citologia , Esôfago/patologia , Mucosa Gástrica/citologia , Mucosa Gástrica/patologia , Histocitoquímica , Humanos , Mucosa Intestinal/citologia , Mucosa Intestinal/patologia , Lesões Pré-Cancerosas/patologia , Raios UltravioletaRESUMO
Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC-CM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSC-CMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the non-invasive purification of PSC-CMs.
Assuntos
Separação Celular/métodos , Citometria de Varredura a Laser/métodos , Miócitos Cardíacos/citologia , Células-Tronco Pluripotentes/citologia , Células Cultivadas , Humanos , Miócitos Cardíacos/metabolismo , Miosinas/metabolismo , Células-Tronco Pluripotentes/metabolismo , Coloração e RotulagemRESUMO
We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems--an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.
Assuntos
Microscopia de Fluorescência/métodos , Espectrometria de Fluorescência/métodos , Animais , Esôfago de Barrett/patologia , Endoscopia/instrumentação , Endoscopia/métodos , Desenho de Equipamento , Mucosa Gástrica/química , Mucosa Gástrica/citologia , Histocitoquímica/instrumentação , Histocitoquímica/métodos , Humanos , Rim/química , Rim/citologia , Camundongos , Microscopia de Fluorescência/instrumentação , Espectrometria de Fluorescência/instrumentação , Espectrofotometria UltravioletaRESUMO
We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.
Assuntos
Técnicas Citológicas/instrumentação , Pinças Ópticas , Análise Espectral Raman/métodos , Desenho de Equipamento , Eritrócitos/citologia , Humanos , MicroesferasRESUMO
The use of injected stem cells, leading to regeneration of ischemic heart tissue, for example, following coronary artery occlusion, has emerged as a major new option for managing 'heart attack' patients. While some clinical trials have been encouraging, there have also been failures and there is little understanding of the multiplicity of factors that lead to the outcome. In this overview paper, the opportunities and challenges in applying biophotonic techniques to regenerative medicine, exemplified by the challenge of stem cell therapy of ischemic heart disease, are considered. The focus is on optical imaging to track stem cell distribution and fate, and optical spectroscopies and/or imaging to monitor the structural remodeling of the tissue and the resulting functional changes. The scientific, technological, and logistics issues involved in moving some of these techniques from pre-clinical research mode ultimately into the clinic are also highlighted.
Assuntos
Transplante de Células-Tronco , Humanos , Medicina Regenerativa , Células-TroncoRESUMO
Shape memory polymer stent prototypes were fabricated from thermoplastic polyurethane. Commercial stents are generally made of stainless steel or other alloys. These alloys are too stiff and prevent most stent designs from being able to navigate small and tortuous vessels to reach intracranial lesions. A solid tubular model and a high flexibility laser etched model are presented. The stents were tested for collapse in a pressure chamber. At 37 degrees C, the full collapse pressure was comparable to that of commercially available stents, and higher than the estimated maximum pressure exerted by intracranial arteries. However, there is a potential for onset of collapse, which needs further study. The stents were crimped and expanded, the laser-etched stent showed full recovery with an expansion ratio of 2.7 and a 1% axial shortening.
Assuntos
Neovascularização Fisiológica , Polímeros , Stents , Modelos Teóricos , PressãoRESUMO
Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.