1.
Biochem Biophys Res Commun
; 405(3): 373-6, 2011 Feb 18.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21219859
RESUMO
Fish models like medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugelp11cv (mzlp11cv) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.