Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Bases de dados
Ano de publicação
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
Front Immunol ; 15: 1392456, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38779673

RESUMO

In response to the global threat posed by bacterial pathogens, which are the second leading cause of death worldwide, vaccine development is challenged by the diversity of bacterial serotypes and the lack of immunoprotection across serotypes. To address this, we introduce BacScan, a novel genome-wide technology for the rapid discovery of conserved highly immunogenic proteins (HIPs) across serotypes. Using bacterial-specific serum, BacScan combines phage display, immunoprecipitation, and next-generation sequencing to comprehensively identify all the HIPs in a single assay, thereby paving the way for the development of universally protective vaccines. Our validation of this technique with Streptococcus suis, a major pathogenic threat, led to the identification of 19 HIPs, eight of which conferred 20-100% protection against S. suis challenge in animal models. Remarkably, HIP 8455 induced complete immunity, making it an exemplary vaccine target. BacScan's adaptability to any bacterial pathogen positions it as a revolutionary tool that can expedite the development of vaccines with broad efficacy, thus playing a critical role in curbing bacterial transmission and slowing the march of antimicrobial resistance.


Assuntos
Proteínas de Bactérias , Animais , Camundongos , Proteínas de Bactérias/imunologia , Proteínas de Bactérias/genética , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus suis/imunologia , Streptococcus suis/genética , Genoma Bacteriano , Sequenciamento de Nucleotídeos em Larga Escala , Feminino , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/genética , Humanos , Vacinas Bacterianas/imunologia
2.
Front Immunol ; 14: 1274027, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38098490

RESUMO

Background: Emerging infectious diseases pose a significant threat to both human and animal populations. Rapid de novo identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens. Methods: Here, we focused on Actinobacillus pleuropneumoniae, which poses a serious threat to the pig industry, and developed a general workflow by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid de novo identification of bacterial protective proteins from a clinical isolate. Results: As a proof of concept, we identified 3 novel protective proteins of A. pleuropneumoniae. Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against A. pleuropneumoniae. Discussion: We believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.


Assuntos
Actinobacillus pleuropneumoniae , Pleuropneumonia , Animais , Humanos , Suínos , Antígenos de Bactérias , Vacinas Bacterianas , Proteínas de Bactérias , Pleuropneumonia/microbiologia , Pleuropneumonia/prevenção & controle
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA