Rats respond to aversive emotional arousal of human handlers with the activation of the basolateral and central amygdala.

Reading danger signals may save an animal's life, and learning about threats from others allows avoiding first-hand aversive and often fatal experiences. Fear expressed by other individuals, including those belonging to other species, may indicate the presence of a threat in the environment and is an important social cue. Humans and other animals respond to conspecifics' fear with increased activity of the amygdala, the brain structure crucial for detecting threats and mounting an appropriate response to them. It is unclear, however, whether the cross-species transmission of threat information involves similar mechanisms, e.g., whether animals respond to the aversively induced emotional arousal of humans with activation of fear-processing circuits in the brain. Here, we report that when rats interact with a human caregiver who had recently undergone fear conditioning, they show risk assessment behavior and enhanced amygdala activation. The amygdala response involves its two major parts, the basolateral and central, which detect a threat and orchestrate defensive responses. Further, we show that humans who learn about a threat by observing another aversively aroused human, similar to rats, activate the basolateral and centromedial parts of the amygdala. Our results demonstrate that rats detect the emotional arousal of recently aversively stimulated caregivers and suggest that cross-species social transmission of threat information may involve similar neural circuits in the amygdala as the within-species transmission.

Development and validation of the Emotional Climate Change Stories (ECCS) stimuli set.

Climate change is widely recognised as an urgent issue, and the number of people concerned about it is increasing. While emotions are among the strongest predictors of behaviour change in the face of climate change, researchers have only recently begun to investigate this topic experimentally. This may be due to the lack of standardised, validated stimuli that would make studying such a topic in experimental settings possible. Here, we introduce a novel Emotional Climate Change Stories (ECCS) stimuli set. ECCS consists of 180 realistic short stories about climate change, designed to evoke five distinct emotions-anger, anxiety, compassion, guilt and hope-in addition to neutral stories. The stories were created based on qualitative data collected in two independent studies: one conducted among individuals highly concerned about climate change, and another one conducted in the general population. The stories were rated on the scales of valence, arousal, anger, anxiety, compassion, guilt and hope in the course of three independent studies. First, we explored the underlying structure of ratings (Study 1; n = 601). Then we investigated the replicability (Study 2; n = 307) and cross-cultural validity (Study 3; n = 346) of ECCS. The collected ratings were highly consistent across the studies. Furthermore, we found that the level of climate change concern explained the intensity of elicited emotions. The ECCS dataset is available in Polish, Norwegian and English and can be employed for experimental research on climate communication, environmental attitudes, climate action-taking, or mental health and wellbeing.

Learning about threat from friends and strangers is equally effective: An fMRI study on observational fear conditioning.

Humans often benefit from social cues when learning about the world. For instance, learning about threats from others can save the individual from dangerous first-hand experiences. Familiarity is believed to increase the effectiveness of social learning, but it is not clear whether it plays a role in learning about threats. Using functional magnetic resonance imaging, we undertook a naturalistic approach and investigated whether there was a difference between observational fear learning from friends and strangers. Participants (observers) witnessed either their friends or strangers (demonstrators) receiving aversive (shock) stimuli paired with colored squares (observational learning stage). Subsequently, participants watched the same squares, but without receiving any shocks (direct-expression stage). We observed a similar pattern of brain activity in both groups of observers. Regions related to threat responses (amygdala, anterior insula, anterior cingulate cortex) and social perception (fusiform gyrus, posterior superior temporal sulcus) were activated during the observational phase, possibly reflecting the emotional contagion process. The anterior insula and anterior cingulate cortex were also activated during the subsequent stage, indicating the expression of learned threat. Because there were no differences between participants observing friends and strangers, we argue that social threat learning is independent of the level of familiarity with the demonstrator.

Decreased preparatory activation and inattention to cues suggest lower activation of proactive cognitive control among high procrastinating students.

Procrastination is a voluntary delay in completing an important task while being aware that this behavior may lead to negative outcomes. It has been shown that an increased tendency to procrastinate is associated with deficits in some aspects of cognitive control. However, none of the previous studies investigated these dysfunctions through the lenses of the Dual Mechanisms Framework, which differentiates proactive and reactive modes of control. The present study was designed to fill this gap, using behavioral and neurophysiological assessment during the completion of the AX-Continuous Performance Task (AX-CPT) by high (HP) and low (LP) procrastinating students (N = 139). Behavioral results indicated that HP (vs. LP) were characterized by increased attentional fluctuations (higher reaction time variability) and reduction in some indices of proactive cognitive control (lower d'-context and A-cue bias, but similar PBIs). Furthermore, the neurophysiological data showed that HP, compared with LP, allocated less attentional resources (lower P3b) to cues that help to predict the correct responses to upcoming probes. They also responded with reduced preparatory activity (smaller CNV) after cues presentation. The two groups did not differ in neural responses linked to conflict detection and inhibition (similar N2 and P3a). Obtained findings indicate that HP might present deficits in some cognitive functions that are essential for effective proactive control engagement, along with preserved levels of reactive cognitive control. In the present paper, we discuss the potential neural and cognitive mechanisms responsible for the observed effects.

The Set of Fear Inducing Pictures (SFIP): Development and validation in fearful and nonfearful individuals.

Emotionally charged pictorial materials are frequently used in phobia research, but no existing standardized picture database is dedicated to the study of different phobias. The present work describes the results of two independent studies through which we sought to develop and validate this type of database-a Set of Fear Inducing Pictures (SFIP). In Study 1, 270 fear-relevant and 130 neutral stimuli were rated for fear, arousal, and valence by four groups of participants; small-animal (N = 34), blood/injection (N = 26), social-fearful (N = 35), and nonfearful participants (N = 22). The results from Study 1 were employed to develop the final version of the SFIP, which includes fear-relevant images of social exposure (N = 40), blood/injection (N = 80), spiders/bugs (N = 80), and angry faces (N = 30), as well as 726 neutral photographs. In Study 2, we aimed to validate the SFIP in a sample of spider, blood/injection, social-fearful, and control individuals (N = 66). The fear-relevant images were rated as being more unpleasant and led to greater fear and arousal in fearful than in nonfearful individuals. The fear images differentiated between the three fear groups in the expected directions. Overall, the present findings provide evidence for the high validity of the SFIP and confirm that the set may be successfully used in phobia research.

[Polish adaptation and validation of the Agoraphobic Cognitions Questionnaire and the Body Sensations Questionnaire]. / Polska adaptacja i walidacja Kwestionariusza Mysli Towarzyszacych Agorafobii (KMTA) oraz Kwestionariusza Doznan Cielesnych (KDC).

AIM: The present study aimed at the adaptation and validation of two questionnaires assessing fear of bodily sensations (BSQ; suggested Polish name: Kwestionariusz Doznan Cielesnych [KDC]) and concerns specific to agoraphobics (ACQ; suggested Polish name: Kwestionariusz Mysli Towarzyszacych Agorafobii [KMTA]). METHOD: The study included a total of 82 patients diagnosed with agoraphobia or panic disorder with agoraphobia according to the diagnostic criteria of the DSM-IV as well as 100 control subjects who did not show the presence of mental disorders. RESULTS: The results showed that both adapted questionnaires meet basic psychometric criteria. The Polish-language versions of the ACQ and BSQ are characterized by a high content validity, internal consistency and showed to be stable over a period of 28 days. Moreover, the factor structure of the Polish version of the ACQ showed to be highly similar to the original version. CONCLUSIONS: Polish-language versions of the ACQ and BSQ have been found to be reliable and valid research and diagnostic instruments for the assessment of fear for bodily sensations and agoraphobic cognitions. Due to their high efficiency and adequate psychometric characteristics these measures might be very useful in research as well as in the diagnosis and evaluation of therapeutic effects.

Improved attention and performance monitoring in high procrastinating students after positive relative to negative norm-referenced feedback.

Procrastination is an irrational delay of task completion. Previous studies have demonstrated that individuals who often procrastinate present deficits in attentional control and performance monitoring and that these dysfunctions might be differentially manifested depending on the motivational context. Building upon these results, the present event-related potential (ERP) study aimed to investigate the impact of norm-referenced feedback on executive functions among students with high (HP; N = 75) or low (LP; N = 77) procrastination levels. Participants completed the parametric Go/No-Go task, while receiving either positive or negative false feedback indicating how well they performed in comparison to others. The results indicated that positive (as opposed to negative) feedback led to higher self-reported arousal and increased post-error slowing in HP (vs. LP) participants. Moreover, neurophysiological measures indicated lower neural activation linked to attentional control (P300) and performance monitoring (ERN, CRN and Pe) in HP than LP participants, while the groups did not differ in these indices during the positive feedback condition. Obtained findings indicate that HP might be more sensitive to the motivating effects of success and more vulnerable to the detrimental influence of failure.

Self-regulation and learning from failures: Probabilistic reversal learning task reveals lower flexibility persisting after punishment in procrastinators.

Procrastination is a self-regulation failure in which people irrationally delay intended actions which leads to lower performance, satisfaction from achievements, and quality of life. Trait procrastination is estimated to affect 15% to 20% of the total population, and previous studies have shown procrastination to be related to impulsivity, emotion dysregulation, and executive dysfunctions, making it a good nonclinical example of a self-regulation disorder. Our previous fMRI results revealed impaired error processing (lower error-related activity of the anterior cingulate cortex) and lack of ability to intensify executive-control during the punishment context (no increase in activity in prefrontal regions) in procrastinators. This led us to the question of whether procrastination is related to impaired learning on errors and punishments. Low (LP) and high (HP) procrastinating students took part in a modified monetary probabilistic reversal learning task with separated reward and punishment conditions. Half of the participants started with reward and half with the punishment condition. Several learning models and model-free measures were applied to the collected behavioral data. Results suggest lower flexibility in the learning task in HP subjects, which can further decrease during the punishment condition. Moreover, HP subjects who began with the punishment condition tended to be less flexible throughout the rest of the task. These results suggest that impaired learning from errors and punishments may prevent highly procrastinating subjects from correcting their behaviors and add to the persistence of procrastination. We also conclude that impaired learning on errors and punishments might be a more general mechanism underpinning other self-regulation disorders. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Kidney transplantation and action-intentional improvements: Evidence from an ERP study.

Reaction slowing observed in dialyzed patients results from deficits in initiating and sustaining motor response mobilization. The present study aimed at investigating whether these deficits are reversible following successful kidney transplantation. To achieve this goal, behavioral and electrophysiological (EEG) data were assessed from healthy control participants as well as kidney transplant and dialyzed patients performing a series of reaction time tasks. The results demonstrated that in patients who received kidney transplant a normalization of response latencies and brain preparatory activity was observed. At the same time, when compared to healthy individuals, increased attention engagement was observed in both clinical groups of patients. No behavioral and electrophysiological indices of impaired monitoring were observed in any of the clinical groups.

Brain potentials reveal reduced attention and error-processing during a monetary Go/No-Go task in procrastination.

Procrastination is a self-regulatory problem of voluntarily and destructively delaying intended and necessary or personally important tasks. Previous studies showed that procrastination is associated with executive dysfunctions that seem to be particularly strong in punishing contexts. In the present event-related potential (ERP) study a monetary version of the parametric Go/No-Go task was performed by high and low academic procrastinators to verify the influence of motivational context (reward vs. punishment expectation) and task difficulty (easy vs. hard) on procrastination-related executive dysfunctions. The results revealed increased post-error slowing along with reduced P300 and error-related negativity (ERN) amplitudes in high (vs. low) procrastination participants-effects that indicate impaired attention and error-related processing in this group. This pattern of results did not differ as a function of task difficulty and motivation condition. However, when the task got more difficult executive attention deficits became even more apparent at the behavioral level in high procrastinators, as indexed by increased reaction time variability. The findings substantiate prior preliminary evidence that procrastinators show difficulties in certain aspects of executive functioning (in attention and error processing) during execution of task-relevant behavior, which may be more apparent in highly demanding situations.

Observational learning of fear in real time procedure.

Learning to avoid threats often occurs by observing others. Most previous research on observational fear learning (OFL) in humans has used pre-recorded standardized video of an actor and thus lacked ecological validity. Here, we aimed to enhance ecological validity of the OFL by engaging participants in a real-time observational procedure (35 pairs of healthy male friends, age 18-27). One of the participants watched the other undergo a differential fear conditioning task, in which a conditioned stimulus (CS+) was paired with an aversive electric shock and another stimulus (CS-) was always safe. Subsequently, the CS+ and CS- were presented to the observer to test the OFL. While the friend's reactions to the shock elicited strong skin conductance responses (SCR) in all observers, subsequent differential SCRs (CS+ > CS-) were found only when declarative knowledge of the CS+/US contingency (rated by the participants) was acquired. Contingency-aware observers also showed elevated fear potentiated startle responses during both CS+ and CS- compared to baseline. We conclude that our real-time procedure can be effectively used to study OFL. The procedure allowed for dissecting two components of the OFL: an automatic emotional reaction to the response of the demonstrator and learning about stimulus contingency.

Brain dynamics in spider-phobic individuals exposed to phobia-relevant and other emotional stimuli.

Dense sensor event-related brain potentials were measured in participants with spider phobia and nonfearful controls during viewing of phobia-relevant spider and standard emotional (pleasant, unpleasant, neutral) pictures. Irrespective of the picture content, spider phobia participants responded with larger P1 amplitudes than controls, suggesting increased vigilance in this group. Furthermore, spider phobia participants showed a significantly enlarged early posterior negativity (EPN) and late positive potential (LPP) during the encoding of phobia-relevant pictures compared to nonfearful controls. No group differences were observed for standard emotional materials indicating that these effects were specific to phobia-relevant material. Within group comparisons of the spider phobia group, though, revealed comparable EPN and LPP evoked by spider pictures and emotional (unpleasant and pleasant) picture contents. These results demonstrate a temporal unfolding in perceptual processing from unspecific vigilance (P1) to preferential responding (EPN and LPP) to phobia-relevant materials in the spider phobia group. However, at the level of early stimulus processing, these effects of increased attention seem to be related to emotional relevance of the stimulus cues rather than reflecting a fear-specific response.

Attenuated brain activity during error processing and punishment anticipation in procrastination - a monetary Go/No-go fMRI study.

Procrastination is a self-regulatory failure in which people voluntarily but irrationally delay important tasks. Trait procrastination is estimated to affect 15-20% of the total population and leads to a significant decrease in performance, satisfaction with achievements, and quality of life. Procrastination is related to impulsivity and reduced executive control, especially in the domain of inhibition. Moreover, procrastinatory tendencies seem to increase with negative affect, suggesting impaired emotion regulation. The aim of this study was to investigate the neuronal mechanisms of inhibition, error processing, and behavioral control under pressure of punishment in procrastinators. Non-student subjects recruited to low (LP) and high procrastination (HP) groups performed an fMRI monetary Go/No-go task. HP showed significantly lower error-related activity in ACC than LP. There was also a significant group by condition interaction in the ACC and right DLPFC suggesting increase of control during the punishment condition in LP but not HP group. These results suggest that procrastinators have impaired error processing mechanisms which may add to the persistence of procrastination through difficulties in correction of faulty behaviors. Procrastination also seems to be related to a decreased ability to intensify self-control in more demanding situations and/or impaired coping in the context of negative situations.

Neural response patterns in spider, blood-injection-injury and social fearful individuals: new insights from a simultaneous EEG/ECG-fMRI study.

In the present simultaneous EEG/ECG-fMRI study we compared the temporal and spatial characteristics of the brain responses and the cardiac activity during fear picture processing between spider, blood-injection-injury (BII) and social fearful as well as healthy (non-fearful) volunteers. All participants were presented with two neutral and six fear-related blocks of pictures: two social, two spider and two blood/injection fear blocks. In a social fear block neutral images were occasionally interspersed with photographs of angry faces and social exposure scenes. In spider and blood/injection fear blocks neutral pictures were interspersed with spider fear-relevant and blood/injection pictures, respectively. When compared to healthy controls the social fear group responded with increased activations in the anterior orbital, middle/anterior cingulate and middle/superior temporal areas for pictures depicting angry faces and with a few elevated superior frontal activations for social exposure scenes. In the blood/injection fear group, heart rate was decreased and the activity in the middle/inferior frontal and visual processing regions was increased for blood/injection pictures. The HR decrease for blood/injection pictures correlated with increased frontal responses. In the spider fear group, spider fear-relevant pictures triggered increased activations within a broad subcortical and cortical neural fear network. The HR response for spider fear-relevant stimuli was increased and correlated with an increased insula and hippocampus activity. When compared to healthy controls, all fear groups showed higher LPP amplitudes for their feared cues and an overall greater P1 hypervigilance effect. Contrasts against the fear control groups showed that the increased responses for fear-specific stimuli are mostly related to specific fears and not to general anxiety proneness. The results suggest different engagement of cognitive evaluation and down-regulation strategies and an overall increased sensitization of the fear system in the three fear groups.

Nencki Affective Picture System: Cross-Cultural Study in Europe and Iran.

Although emotions have been assumed conventionally to be universal, recent studies have suggested that various aspects of emotions may be mediated by cultural background. The purpose of our research was to test these contradictory views, in the case of the subjective evaluation of visual affective stimuli. We also sought to validate the recently introduced Nencki Affective Picture System (NAPS) database on a different cultural group. Since there has been, to date, no attempt to compare the emotions of a culturally distinct sample of Iranians with those of Europeans, subjective ratings were collected from 40 Iranians and 39 Europeans. Each cultural group was asked separately to provide normative affective ratings and classify pictures according to discrete emotions. The results were analyzed to identify cultural differences in the ratings of individual images. One hundred and seventy NAPS pictures were rated with regard to the intensity of the basic emotions (happiness, sadness, fear, surprise, anger, and disgust) they elicited, as well as in terms of affective dimensions (valence and arousal). Contrary to previous studies using the International Affective Picture System, our results for Europeans and Iranians show that neither the ratings for affective dimensions nor for basic emotions differed across cultural groups. In both cultural groups, the relationship between valence and arousal ratings could be best described by a classical boomerang-shaped function. However, the content of the pictures (animals, faces, landscapes, objects, or people) had a significant effect on the ratings for valence and arousal. These findings indicate that further studies in cross-cultural affective research should control for the content of stimuli.

What Is the Effect of Basic Emotions on Directed Forgetting? Investigating the Role of Basic Emotions in Memory.

Studies presenting memory-facilitating effect of emotions typically focused on affective dimensions of arousal and valence. Little is known, however, about the extent to which stimulus-driven basic emotions could have distinct effects on memory. In the present paper we sought to examine the modulatory effect of disgust, fear, and sadness on intentional remembering and forgetting using widely used item-method directed forgetting (DF) paradigm. Eighteen women underwent fMRI scanning during encoding phase in which they were asked either to remember (R) or to forget (F) pictures. In the test phase all previously used stimuli were re-presented together with the same number of new pictures and participants had to categorize them as old or new, irrespective of the F/R instruction. On the behavioral level we found a typical DF effect, i.e., higher recognition rates for to-be-remembered (TBR) items than to-be-forgotten (TBF) ones for both neutral and emotional categories. Emotional stimuli had higher recognition rate than neutral ones, while among emotional those eliciting disgust produced highest recognition, but at the same time induced more false alarms. Therefore, when false alarm corrected recognition was examined the DF effect was equally strong irrespective of emotion. Additionally, even though subjects rated disgusting pictures as more arousing and negative than other picture categories, logistic regression on the item level showed that the effect of disgust on recognition memory was stronger than the effect of arousal or valence. On the neural level, ROI analyses (with valence and arousal covariates) revealed that correctly recognized disgusting stimuli evoked the highest activity in the left amygdala compared to all other categories. This structure was also more activated for remembered vs. forgotten stimuli, but only in case of disgust or fear eliciting pictures. Our findings, despite several limitations, suggest that disgust have a special salience in memory relative to other negative emotions, which cannot be put down to differences in arousal or valence. The current results thereby support the suggestion that a purely dimensional model of emotional influences on cognition might not be adequate to account for observed effects.

Slowing with end-stage renal disease: Attentive but unprepared to act.

Dialyzed patients show longer response latencies to stimuli than healthy controls. This study was designed to learn if this abnormal latency may be related to an impairment in the networks that mediate the allocation of sensory-attention (inducing a delay in stimulus recognition-awareness) and/or an impairment of intentional motor preparation (inducing a delay in action-initiation). Dialyzed patients with end-stage renal disease and matched healthy controls were assessed using reaction time tasks from the ROtman-Baycrest Battery to Investigate Attention (ROBBIA) and event-related potentials (ERPs) to help elucidate the possible attentional and/or intentional brain mechanisms that may account for this slowing. The following ERP components were analyzed for single attentional and intentional processes: response preparation (Contingent Negative Variation); perceptual preparation (P1); selective attention and monitoring (P300/Late Positive Potential). Patients (vs. controls) had a decreased ability to sustain response preparation under the condition of a long (3 s) but not a short (1 s) preparation period. This action-intentional deficit was, however, not accompanied by impaired perceptual preparation and monitoring. Future research may investigate whether deficits observed in dialyzed patients can be reduced with treatments such as kidney transplantation.

Arousal Rather than Basic Emotions Influence Long-Term Recognition Memory in Humans.

Emotion can influence various cognitive processes, however its impact on memory has been traditionally studied over relatively short retention periods and in line with dimensional models of affect. The present study aimed to investigate emotional effects on long-term recognition memory according to a combined framework of affective dimensions and basic emotions. Images selected from the Nencki Affective Picture System were rated on the scale of affective dimensions and basic emotions. After 6 months, subjects took part in a surprise recognition test during an fMRI session. The more negative the pictures the better they were remembered, but also the more false recognitions they provoked. Similar effects were found for the arousal dimension. Recognition success was greater for pictures with lower intensity of happiness and with higher intensity of surprise, sadness, fear, and disgust. Consecutive fMRI analyses showed a significant activation for remembered (recognized) vs. forgotten (not recognized) images in anterior cingulate and bilateral anterior insula as well as in bilateral caudate nuclei and right thalamus. Further, arousal was found to be the only subjective rating significantly modulating brain activation. Higher subjective arousal evoked higher activation associated with memory recognition in the right caudate and the left cingulate gyrus. Notably, no significant modulation was observed for other subjective ratings, including basic emotion intensities. These results emphasize the crucial role of arousal for long-term recognition memory and support the hypothesis that the memorized material, over time, becomes stored in a distributed cortical network including the core salience network and basal ganglia.

Brain dynamics of visual attention during anticipation and encoding of threat- and safe-cues in spider-phobic individuals.

This study systematically investigated the sensitivity of the phobic attention system by measuring event-related potentials (ERPs) in spider-phobic and non-phobic volunteers in a context where spider and neutral pictures were presented (phobic threat condition) and in contexts where no phobic but unpleasant and neutral or only neutral pictures were displayed (phobia-irrelevant conditions). In a between-group study, participants were assigned to phobia-irrelevant conditions either before or after the exposure to spider pictures (pre-exposure vs post-exposure participants). Additionally, each picture was preceded by a fixation cross presented in one of three different colors that were informative about the category of an upcoming picture. In the phobic threat condition, spider-phobic participants showed a larger P1 than controls for all pictures and signal cues. Moreover, individuals with spider phobia who were sensitized by the exposure to phobic stimuli (i.e. post-exposure participants) responded with an increased P1 also in phobia-irrelevant conditions. In contrast, no group differences between spider-phobic and non-phobic individuals were observed in the P1-amplitudes during viewing of phobia-irrelevant stimuli in the pre-exposure group. In addition, cues signaling neutral pictures elicited decreased stimulus-preceding negativity (SPN) compared with cues signaling emotional pictures. Moreover, emotional pictures and cues signaling emotional pictures evoked larger early posterior negativity (EPN) and late positive potential (LPP) than neutral stimuli. Spider phobics showed greater selective attention effects than controls for phobia-relevant pictures (increased EPN and LPP) and cues (increased LPP and SPN). Increased sensitization of the attention system observed in spider-phobic individuals might facilitate fear conditioning and promote generalization of fear playing an important role in the maintenance of anxiety disorders.