RESUMO
In this investigation, the study focused on the chemical constitution and the antioxidative as well as anti-inflammatory characteristics of oils and pulpy variants (Imatchan (IM), Harmocha (HA), and Aknari (AK)) sourced from O. dillenii. This inquiry encompassed both in vitro and in silico analyses. High-performance liquid chromatography (HPLC) was employed to ascertain the phenolic constituents, while gas chromatography-mass spectrometry (GC-MS) methodologies. were applied to discern the volatile makeup. The appraisal of antioxidant potential was conducted via the deployment of assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferric ion chelating (FIC) techniques. The anti-inflammatory activity was examined using BSA and LOX. Molecular docking methods assessed the antioxidant and anti-inflammatory properties. According to HPLC findings, the most abundant compounds detected in AKO and IMO cultivars were quercetin 3-O-ß-D-glucoside followed by vanillic acid, ferulic acid and tyrolsol. Concerning headspace GC-MS analysis E-11-hexadecenal and (E)-2-undecenal contribute to the major compounds detected in Opuntia HA, IM, and AK pulp and oil. The DPPH IC50 for AK, HA and IM were 38.41±1.54, 42.24±0.29 and 15.17±1.28â mg/mL, respectively. The FRAP IC50 capacity of AK, HA and IM was determined to be 30.23±0.6, 55.96±0.08 and 23.41±1.83â mg/mL, respectively. AK, HA and IM displayed significant FIC activity, with IC50 values of 42.75±0.63, 39.54±0.59 and 35.31±1.38â mg/mL, respectively. The AK, HA and IM O. dillenii oils were effective in their anti-inflammatory activity. Molecular docking of O. dillenii oils phenolic compounds was conducted to determine the possible targeted proteins by the phenolic compounds in O. dillenii's compounds. Overall, these fruits demonstrated the potential for new ingredients for culinary or pharmaceutical applications, providing value to these natural species that can flourish in arid conditions.
Assuntos
Antioxidantes , Opuntia , Antioxidantes/farmacologia , Antioxidantes/química , Opuntia/química , Simulação de Acoplamento Molecular , Extratos Vegetais/química , Anti-Inflamatórios/farmacologia , Fenóis/farmacologia , ÓleosRESUMO
An inventory of Lepidoptera in the Theniet El Had National Park (PNTEH), Algeria, revealed 86 taxa, both butterflies and moths. The specimens were collected in 68 localities distributed over ten cantons within the park in the period 2015-2017. A preliminary faunistic list is compiled as a base-line contribution to the study of adult Lepidoptera in this park. In total, 3139 specimens were collected. The moths are clearly well diversified, with 14 families and 49 species obtained from a total of 1485 adult specimens. The butterflies are represented by 5 families with 37 species and 1654 specimens. A total of 8 families are reported for the first time from this park, in order of abundance: Zygaenidae, Hesperiidae, Crambidae, Alucitidae, Heterogynidae, Sesiidae, Oecophoridae, and Cossidae. Also 61 species are recorded here for the first time for the park. The most diverse family is Nymphalidae with 15 taxa (23% of the total species). On the other hand, the Erebidae are represented by 894 specimens (28.5% of the total number of specimens. Within the Erebidae, the genus Catocala contains the highest number of individuals (794 specimens). The canton of Pré-Ben Chouhra is quantitatively the best represented with 625 specimens (19.9% of the total number of specimens collected) and the Nursery canton as the richest in lepidopteran species with 72 species observed. The diversity indices (H' and Hmax.) and the equitability index (E), calculated for the 10 cantons indicate that lepidopteran species are diverse in each station.