A Novel Three-Dimensional-Printed Ultrasound-Guided Hip Arthrocentesis Model.

When evaluating patients with hip pain, clinicians may be trained to both evaluate for a hip effusion and perform ultrasound-guided arthrocentesis to evaluate the etiology of the effusion. We present a novel 3-dimensional-printed hip arthrocentesis model, which can be used to train clinicians to perform both tasks under ultrasound guidance. Our model uses a combination of a 3-dimensional-printed hip joint, as well as readily available materials such as an infant Ambu (Ballerup, Denmark) bag, syringe, intravenous line kit, and silicone. We present our experience so that others may use and adapt our model for their training purposes.

The Atlantic salmon interleukin 4/13 receptor family: Structure, tissue distribution and modulation of gene expression.

Interleukin (IL)-4 and IL-13 play a central role in T helper 2 immune response in mammals. The cell signalling is mediated by the type I heterodimeric receptor containing the IL-4Rα and γC chains, and the type II receptors formed by IL-4Rα and IL-13Rα1. In salmonid species, three paralogues of the IL-4 and IL-13 cytokines have been reported, il-4/13a, il-4/13b1 and il-4/13b2. In regard to receptors, two paralogues of each IL-4/13 receptor chains have been identified in rainbow trout while five genes named γc1, il-4rα, il-13rα1a, il-13rα1b, and il-13rα2 have identified in Atlantic salmon. Since Atlantic salmon is an important farmed fish species, the aim of this work was to get new insights into distribution, structure and expression regulation of the IL-4/13 receptors in salmon. By using qRT-PCR, it was shown that all γc1, il-4rα, il-13rα1a, il-13rα1b, and il-13rα2 receptor chains were expressed in lymphoid and non-lymphoid tissues of healthy salmon, nonetheless γC expression was higher in lymphoid than non-lymphoid tissues. The in silico structural analysis and homology modelling of the predicted receptor proteins showed that domains and most motifs present in the superior vertebrate chains are conserved in salmon suggesting a conserved role for these receptor chains. Only IL-13Rα1B is a receptor chain with a unique structure that seem not to be present in higher vertebrates but in fish species. In order to determine the regulatory role of IL-4/13 on the expression of receptor chains, Atlantic salmon il-4/13A gene was synthetized and cloned in pET15b. The recombinant IL-4/13A was produced in E. coli and the activity of the purified cytokine was confirmed in vitro. The regulatory role of IL-4/13A on the expression of their potential receptors was tested in salmon receiving the recombinant cytokine and effects were compared with those of the control group. The results showed that IL-4/13A induced the expression of its own gene and GATA-3, in the head kidney of fish but not in the spleen, while IL-10 increased in both lymphoid organs indicating a regulatory role of this cytokine on the induction of Th2 responses in salmon. IFN-γ and MHC class II were also later induced in head kidney. In regard to the expression of the receptor chains, IL-4/13A upregulated the expression of γC, IL-13Rα1A and IL-13Rα2A in the spleen but not in the head kidney of salmon, indicating a role on the modulation of cell signalling for the Th2 response. Furthermore, Piscirickettsia salmonis infection of Atlantic salmon occurred with an increase of γC and IL-13Rα1A suggesting a potential role of the IL-4/13 system in bacterial immunity or pathogenesis. This study contributes to a better understanding of the IL-4/13A system in salmon, which as a key axis for Th2 response may be involved not only in pathogen elimination but also in adaptive immune repair that seems critical tolerance to infectious diseases.

Contemporary practice patterns related to the risk of acute kidney injury in the catheterization laboratory: Results from a survey of Society of Cardiovascular Angiography and Intervention (SCAI) cardiologists.

OBJECTIVES: The goal of the present study was to survey the Society of Cardiovascular Angiography and Intervention (SCAI) member cardiologists to evaluate contemporary practice patterns with regards to contrast use, acute kidney injury (AKI) risk assessment, and prevention in patients undergoing invasive angiography. We sought to compare the physician responses against guideline statements and evidence-based data from clinical studies. METHODS: A 20-question online survey based on a modified Likert scale was sent out via email to the Society of Cardiovascular Angiography and Intervention (SCAI) member cardiologists. The survey questions focused on prophylaxis methods, medication management, risk assessment, contrast agent use, and postprocedure care. A scoring system was developed which examined the individual responses to analyze the 10 questions with the greatest strength of evidence in the literature and guidelines. RESULTS: The survey was completed by 506 individuals. Selected responses of note included the use of standardized volume expansion protocols: 64.8%, use of iso-osmolar contrast (iodixanol) in the majority of patients at risk of AKI: 55%, and 27% of individuals reported diluting contrast with saline for patients at risk of AKI during coronary angiography. For questions with support from guideline documents, 56.9% of the responses were scored as concordant with evidence-based data. Individuals who reported that the risk of AKI was often or always important in planning angiography for "at risk patients" were more likely to closely monitor renal function (76.7% vs. 40.0%, P = 0.003), obtain nephrology consultation (45.2% vs. 13.3%, P = 0.016) and use iso-osmolar contrast agents (56.0% vs. 26.7%, P = 0.033). CONCLUSIONS: The majority of cardiologists participating in this survey, reported practice patterns consistent with guideline and evidence-based recommendations. However, over 40% of responses to questions were inconsistent with these recommendations, suggesting continued opportunities for education and quality improvement concerning AKI prevention. © 2016 Wiley Periodicals, Inc.

Echocardiographic screening in pediatric asymptomatic or paucisymptomatic coronavirus disease 2019 outpatients: is it a useful test or an excess of zeal?

INTRODUCTION: Data regarding echocardiographic findings during follow-up of asymptomatic or pauci-symptomatic coronavirus disease 2019 (COVID-19) are scarce in pediatric patients. The aim of the present study is to assess post-COVID-19 sequelae through echocardiography in children who have experienced mild SARS-CoV-2. METHODS: This single-center, retrospective, observational study enrolled a cohort of 133 pediatric outpatients, born between 2005 and 2022, with a history of asymptomatic or paucisymptomatic SARS-CoV-2 infection, who underwent transthoracic echocardiographic (TTE) evaluation at an outpatient pediatric clinic in Northern Italy. RESULTS: The percentage of the pediatric activity of the clinic which was focused on post-COVID evaluation was not negligible, representing almost 10% of the ∼1500 pediatric patients examined from 1 January 2021 to 31 August 2022. According to ACEP classification, children enrolled in this study had previously experienced in 72.9% (97) asymptomatic COVID-19 and nearly 27% (36) a mild illness. Clinical and instrumental examinations did not show any relevant abnormality in the functional [left ventricular ejection fraction (LVEF), tricuspid annular plane systolic excursion (TAPSE), pulmonary artery systolic pressure (PASP)] or structural [interventricular septum diameter (IVSd), left ventricular internal diameter (LViD, end-diastolic volume (EDV), left atrium volume (LAV)] parameters examined related to SARS-CoV-2 infection in the total of 133 children. CONCLUSION: According to our results, children who experienced an asymptomatic or mild SARS-CoV-2 infection should not be systematically investigated with second-level techniques, such as TTE, in the absence of clinical suspicion or other risk conditions such as congenital heart diseases, comorbidities or risk factors.

Housing fuzzy recommender system: A systematic literature review.

In recent years, significant attention has been paid to fuzzy recommender systems for housing, highlighting their ability to effectively handle the imprecision and uncertainty inherent in the real estate market. With the objective of improving the filtering of recommendations in the real estate sector, the PRISMA 2020 methodology was applied to perform new systematic reviews using its checklist on six academic databases from 1985 to 2024. RawGraph, Orange Data Minig, Jamovi and R software were used for document classification and data visualization. After classification, 1003 articles were obtained, of which 46.36% were in Scopus, and 57.82% were articles. At the end of the type, 50 articles were identified as primary, subjecting them to six research questions. It was found that 65% of the algorithms used fuzzy logic, 60% used spatial data, and 80% evaluated performance. The main difficulties were related to the integration of various sources of information. Although incorporating reclusive methods is anticipated in future systems, the need remains to address challenging areas to improve the overall performance of fuzzy recommender systems. The reviewed articles focus on enhancing fuzzy data-based recommendation systems by proposing flexible and less intrusive techniques. The significance of incorporating contextual information and exploring hybrid approaches is emphasized, along with the evaluation in real world environments, averaging artificial intelligence.

Prevalence of associated factors on depression during COVID 19 in students in a minority serving institution: A cross sectional study.

Introduction: The COVID-19 pandemic changed the learning style of university students in the US, affecting their mental health of students. This study aims to understand the factors that influenced depression during the COVID-19 pandemic in the New Mexico State University (NMSU) student population. Methods: A questionnaire assessing mental health and lifestyle factors was delivered to NMSU students by using QualtricsXM software. Depression was assessed using the Patient Health Questionnaire- 9 (PHQ-9); depression was defined as a score ≥10. Single and multifactor logistic regression was performed using R software. Results: This study determined that the prevalence of depression among female students was 72% and 56.30% among male students. Several covariates were significant for increased odds of depression in students, including decreased diet quality (OR: 5.126, 95% CI: 3.186-8.338), annual household income $10,000 - $20,000 (OR: 3.161, 95% CI: 1.444-7.423), increased alcohol consumption (OR: 2.362, 95% CI: 1.504-3.787), increased smoking (OR: 3.581, 95% CI:1.671-8.911), quarantining due to COVID (OR: 2.001, 95% CI: 1.348-2.976), and family member dying of COVID (OR: 1.916, 95% CI: 1.072-3.623). Covariates of being male (OR: 0.501, 95% CI: 0.324-0.776), married (OR: 0.499, 95% CI: 0.318-0.786), eating a balanced diet (OR: 0.472, 95% CI: 0.316-0.705), and sleeping 7-8 h per night (OR: 0.271, 95% CI: 0.175-0.417) were all protective factors for depression in NMSU students. Limitation: This is a cross-sectional study, and therefore, causation cannot be determined. Conclusion: Several factors regarding demographics, lifestyle, living arrangements, alcohol and tobacco use, sleeping behavior, family vaccination, and COVID status were significantly associated with depression in students during the COVID-19 pandemic.

Efecto de la utilización de harina de vísceras de pollo en los parámetros productivos de lechones destetados / Effect of the use of chicken viscera meal on the productive parameters of weaned piglets / Efeito da utilização de farinha de vísceras de frango nos parâmetros produtivos de leitões desmamados

Resumen En la alimentación de cerdos el costo significativo esta influenciado por el uso de alimentos balanceados, por ende, se evaluó el uso de la harina de vísceras de pollo (HV) en lechones destetados y el efecto sobre los parámetros productivos. Se utilizó 36 lechones machos y hembras de 28 días de edad, distribuidos en tres tratamientos diferentes: T1 con pienso comercial (PC), T2 con el 50% de PC y 50% de harina de vísceras (HV), T3 con 0% PC y 100% HV. Se evaluó el consumo alimenticio, ganancia media diaria de peso, conversión alimenticia, rentabilidad, morbilidad, mortalidad, mediante la prueba de Anova y para ver que tratamiento fue significativo donde se utilizó la prueba de comparación múltiple de Tukey, por otro lado, se utilizó el algoritmo de árbol de decisión para ver que tratamiento es más rentable al utilizar todas las variables entrada. Se comprobó que existen diferencias significativas con respecto a: peso corporal, ganancia de peso, consumo de alimento, conversión alimenticia al 95% de significancia. Mediante el algoritmo de árbol de decisión el tratamiento de mayor rentabilidad es T2. En base a los resultados, la harina de vísceras de pollo puede ser utilizada al 100% en lechones destetados, pero en cuanto a la rentabilidad es T2 por su aporte en lo que respecta a producción de carne por kg y bajo costo comercial.

Abstract In pig feeding, the significant cost is influenced by the use of balanced feed, therefore, the use of chicken viscera (HV) meal in weaned piglets and the effect on productive parameters was evaluated. Thirty-six 28-day-old male and female piglets were used, distributed in three different treatments: T1 with commercial feed (PC), T2 with 50% PC and 50% viscera meal (HV), T3 with 0% PC and 100% HV. Food consumption, average daily weight gain, feed conversion, profitability, morbidity, mortality, were evaluated through the Anova test and to see which treatment was significant where Tukey's multiple comparison test was used, on the other hand, it was used the decision tree algorithm to see which treatment is more profitable when using all the input variables. It was found that there are significant differences with respect to: body weight, weight gain, feed consumption, feed conversion at 95% significance. Using the decision tree algorithm, the most profitable treatment is T2. Based on the results, chicken viscera meal can be used 100% in weaned piglets, but in terms of profitability it is T2 for its contribution in terms of meat production per kg and low commercial cost.

Resumo Na alimentação de suínos, o custo significativo é influenciado pelo uso de ração balanceada, portanto, avaliou-se o uso de farinha de vísceras de frango (HV) em leitões desmamados e o efeito sobre parâmetros produtivos. Foram utilizados 36 leitões machos e fêmeas com 28 dias de idade, distribuídos em três diferentes tratamentos: T1 com ração comercial (PC), T2 com 50% PC e 50% farinha de vísceras (HV), T3 com 0% PC e 100% HV. O consumo alimentar, ganho de peso médio diário, conversão alimentar, lucratividade, morbidade, mortalidade, foram avaliados através do teste Anova e para verificar qual tratamento foi significativo onde foi utilizado o teste de comparação múltipla de Tukey, por outro lado, foi utilizado o algoritmo de árvore de decisão para ver qual tratamento é mais lucrativo ao usar todas as variáveis ​​de entrada. Verificou-se que existem diferenças significativas com relação a: peso corporal, ganho de peso, consumo de ração, conversão alimentar a 95% de significância. Usando o algoritmo de árvore de decisão, o tratamento mais rentável é o T2. Com base nos resultados, a farinha de vísceras de frango pode ser utilizada 100% em leitões desmamados, mas em termos de rentabilidade é T2 por sua contribuição em termos de produção de carne por kg e baixo custo comercial.

Comparación entre manguera difusora y tubería PVC para la aireación en la producción de Litopenaeus vannamei con biofloc a diferentes niveles de proteína / Comparison between diffuser hose and PVC pipe for aeration in production of Litopenaeus vannamei with biofloc at different protein levels / Comparação entre mangueira difusora e tubo de PVC para aeração na produção de Litopenaeus vannamei com bioflocos em diferentes níveis de proteína

Resumen Al implementar la tecnología biofloc, la aireación es uno de los problemas para obtener buenos resultados productivos, por tanto, el objetivo fue comparar la eficiencia de la tubería PVC y manguera difusora, como sistema de aireación en cultivos con biofloc y dos niveles de proteína. Se aplicó un diseño completamente al azar con arreglo factorial con cuatro tratamientos, tres réplicas cada uno, con los dos sistemas de aireación y dos niveles de proteína bruta (PB) en el alimento balanceado en producciones de camarón Litopenaeus vannamei, distribuidos de la siguiente manera: para el tratamiento uno (T1) se utilizó tubería PVC y 28% de PB; para T2, manguera difusora y 22% de PB; para T3, manguera difusora y 28% de PB; para T4, tubería PVC y 22% de PB, todos producidos con biofloc. Las variables peso, talla, alimentación y el factor tratamiento influencian significativamente en la concentración de oxígeno disuelto (OD, mg/l) en la tarde, T1 es el que mejor se comportó en la tarde y T3 en la mañana. El tratamiento con menor sedimentación a los 20 y 30 minutos en la prueba de cono Imhoff fue T4 y el de mayor sedimentación fue el T3. No hubo diferencia significativa en la supervivencia entre tratamientos, los niveles de proteína sí influencian en las concentraciones del OD. El uso de tubería PVC aporta en el incremento de peso y de la concentración de OD en relación al sistema de manguera difusora con el 22% y 28% de proteína bruta.

Abstract When implementing biofloc technology, aeration is one of the problems to obtain good productive results, therefore, the objective was to compare the efficiency of PVC pipe and diffuser hose, as an aeration system in crops with biofloc and two levels of protein. A completely randomized design with factorial arrangement was applied with four treatments with three replicates each, with the two aeration systems and with two levels of crude protein (CP) in the balanced feed in Litopenaeus vannamei shrimp productions, distributors of the following way, for treatment one (T1) PVC pipes and 28% PB were achieved; for T2, diffuser hose and 22% PB; for T3, diffuser hose and 28% PB; for T4, PVC pipe and 22% PB, produced with biofloc. The variables weight, height, feeding and the treatment factor significantly influence the concentration of dissolved oxygen (DO, mg/l) in the afternoon. T1 is the one that behaves best in the afternoon and T3 in the morning, the treatment with the lowest sedimentation at 20 and 30 minutes in the Imhoff cone test was T4 and the highest sedimentation was T3. There was no significant difference in survival between treatments, protein levels did influence OD concentrations. The use of PVC pipe provides an increase in weight and DO concentration in relation to the diffuser hose system with 22% and 28% crude protein.

Resumo Ao implementar a tecnologia de bioflocos, a aeração é um dos problemas para obter bons resultados produtivos, portanto, objetivou-se comparar a eficiência do tubo de PVC e da mangueira difusora, como sistema de aeração em lavouras com bioflocos e dois níveis de proteína. Foi aplicado um delineamento inteiramente casualizado com arranjo fatorial com quatro tratamentos com três repetições cada, com os dois sistemas de aeração e com dois níveis de proteína bruta (PB) na ração balanceada em produções de camarão Litopenaeus vannamei, distribuidores da seguinte forma, para tratamento com tubo de PVC (T1) e PB de 28%; para T2, mangueira difusora e PB 22%; para T3, mangueira difusora e PB 28%; para T4, tubo de PVC e PB 22%, produzido com biofloco. As variáveis peso, altura, alimentação e o fator de tratamento influenciam significativamente na concentração de oxigênio dissolvido (OD, mg/l) no período da tarde, T1 é o que apresenta melhor desempenho no período da tarde e T3 no período da manhã, tratamento com menor sedimentação aos 20 e 30 minutos no teste do cone de Imhoff foi T4 e a maior sedimentação foi T3. Não houve diferença significativa na sobrevivência entre os tratamentos, os níveis de proteína influenciaram as concentrações de DO. A utilização de tubo de PVC proporciona um aumento de peso e concentração de OD em relação ao sistema de mangueiras difusoras com 22% e 28% de proteína bruta.

Dead Tumor Cells Expressing Infectious Salmon Anemia Virus Fusogenic Protein Favor Antigen Cross-Priming In Vitro.

Antigen cross-presentation is a crucial step in the assembly of an antitumor immune response leading to activation of naïve CD8 T cells. This process has been extensively used in clinical trials, in which dendritic cells generated in vitro are loaded with tumor antigens and then autotransplanted to the patients. Recently, the use of autologous transplant of dendritic cells fused with dying tumor cells has demonstrated good results in clinical studies. In this work, we generated a similar process in vivo by treating mice with dead tumor cells [cell bodies (CBs)] expressing the fusogenic protein of the infectious salmon anemia virus (ISAV). ISAV fusion protein retains its fusogenic capability when is expressed on mammalian cells in vitro and the CBs expressing it facilitates DCs maturation, antigen transfer by antigen-presenting cells, and increase cross-presentation by DCs in vitro. Additionally, we observed in the melanoma model that CBs with or without ISAV fusion protein reduce tumor growth in prophylactic treatment; however, only ISAV expressing CBs showed an increase CD4 and CD8 cells in spleen. Overall, our results suggest that CBs could be used as a complement with other type of strategies to amplify antitumor immune response.