Correlation of MT levels in livers and gills with heavy metals in wild tilapia (Oreochromis mossambicus) from the Klang River, Malaysia.

Metallothionein (MT) concentrations in gill and liver tissues of Oreochromis mossambicus were determined to assess biological response of fish to levels of some metals. Metal concentrations in gill and liver tissues of O. mossambicus ranged from 0.6 to 2.6 for Cd, 16 to 52 for Zn, 0.5 to 17 for Cu and 2 to 67 for T-Hg (all in µg/g wet weight, except for T-Hg in ng/g wet weight). Accumulation of Cd, Zn, Cu and Hg (µg/g wet weight) in the liver and gills of O. mossambicus were in the order of liver > gills. The concentrations of Cd, Zn, Cu and Hg in fish tissues were correlated with MT content. The increases in MT levels from the reference area Puchong Tengah compared to the polluted area Kampung Seri Kenangan were 3.4- and 3.8-fold for gills and livers, respectively. The results indicate that MT concentrations were tissue-specific, with the highest levels in the liver. Therefore, the liver can act as a tissue indicator in O. mossambicus in the study area.

Distribution, risk assessment, and source identification of trace metal pollution along the Babolsar coastal area, Caspian Sea.

The Caspian Sea is exposed to numerous anthropogenic activities such as untreated wastewater discharge and agricultural activities which increased trace metals contamination. The current study was employed to assess the distribution, ecological risk assessment, and source identification of some trace metals in 125 samples of surface sediments and soil from 6 distinctive sections of Babolsar in the coastal line of the Caspian Sea. The sediment quality guidelines (SQGs) and individual risk assessment indices including enrichment factor (EF), geo-accumulation index (Igeo), contamination factor (Cf), and potential risk factor (Er) suggested a hazardous level of Cd and Cr contamination. There was a relatively high level of Pb contamination while other studied trace metals were at a low contamination level. Cumulative risk indices such as modified degree of contamination (mCD), pollution load index (PLI), and the potential ecological risk index (RI) identified that the river, wetland, and farmland sites were more contaminated compared to the Caspian Sea samples which exhibited a moderate level of contamination. The lowest level of contamination was recorded in the coastline and river delta sites. Two analytical methods including Pearson's correlation coefficient and multivariate clustering dendrogram were also applied to identify the potential sources of contamination. The results suggested that wastewaters, nitrogen, and phosphate fertilizers were the main anthropogenic source of Cd, Co, Pb, and Ni while fossil fuels and transportation activities were the predominant sources of anthropogenic Cu, V, and Zn contamination. Further studies about trace metals risk assessment and fractionation could contribute to more effective decisions for reducing the anthropogenic trace metal pollution in the Caspian Sea.

Microplastics in wastewater outlets of Bandar Abbas city (Iran): A potential point source of microplastics into the Persian Gulf.

Wastewater discharge is considered to be a significant point source of microplastic (MPs) release into the marine environment. This study is the first attempt to quantify MPs released from the wastewater outfall from Bandar Abbas City into the Persian Gulf. Two wastewater discharge stations at Gursuzan and Suru were sampled. MPs were isolated by an oxidative procedure and subsequent density separation using ZnCl2 solution. The average MP concentration in wastewater and sludge were 70.66 (±14.12, SD) MP.35 L-1 and 6070 (±807.25) MPs.kg-1, respectively at Confidence Level (CL) (95.0%). The most commonly recovered polymers were polyethylene (PE) and polypropylene (PP) in all size classes. Our findings provides a baseline of MP concentration in wastewater streams and slurry that is discharged from the Bandar Abbas wastewater treatment facility into the Persian Gulf. This highlights the need to undertake more studies at water treatment plants in the region for a realistic assessment of MP discharge into the Persian Gulf.

A review of microplastic distribution in sediment profiles.

The growing use of plastic and their indiscriminate disposal into the environment has resulted in the accumulation of huge quantities of microplastic (MP) in the marine environment. Several studies have investigated the pathways of MPs reaching marine environments and a few have begun looking at their inventories in sediment profiles. Recent estimates suggest between 70 and 90% of the MP particles are accumulated into sediment profiles. This paper reviews MPs in sediment cores, and the methods used for chronological reconstruction to provide evidence to link the plastic usage and MP inventories over time. In one example, a 210Pb dated sediment core from Kuwait Bay revealed 0.1 MPs g-1 of sediment deposited in approximately 1951 and 0.5 MPs g-1 in sediment from 2009 indicating a progressively increasing presence in recent sediments. The review also highlights the issue of very large spatio-temporal variation in MP concentrations, indicating the limitation of a snapshot study especially when dealing with the surface sediments; and it demonstrates the relevance of using sediment cores for a better assessment of MP input and inventories in the marine environment. It is proposed that MP sediment data should be reported as N m-2 year-1 or g m-2 year-1, and cores preferably dated using 210Pb, and supported by at least one other stratigraphic marker to enhance the usefulness of these datasets, since 210Pb can reliably date sediments as old as 100 years, a time-span which extends well beyond the time plastic production began. The anomalies in data processing and reporting in terms of number per kg of sediment or per cm2/m2 should be addressed essentially by adopting a standardized protocol.

Speciation and risk assessment of selected trace metals in bottom sediment of coral reef ecosystems of the Persian Gulf.

To evaluate the hazard assessment of anthropogenic activities on coastal ecosystems, fractionation and bioaccumulation of trace metals were carried out for sediment and coral samples of three distinct habitats including petroleum exploration area of Kharg, the rural harbor of Chirouyeh, and unpopulated area of Hendorabi. Fractionation results suggested that Ni (~ 51%), Pb (~ 49%), and V (~ 45%) in Kharg; Ni (~ 46%), Pb (~ 84%), and Zn (~ 47%) in Chirouyeh; and Cd (~ 51%) in Hendorabi were the predominant metals in the non-resistant fractions of the sediment samples. Risk Assessment Code (RAC), individual contamination factor (ICF), and global contamination factor (GCF) were derived. The highest relative risk for Cd, Cu, and Ti was observed in Kharg, whereas the highest relative risk for Ni, Pb, V, and Zn was recorded in Chirouyeh. The lowest risk for almost all of the trace metals was observed in Hendorabi. Biota-sediment accumulation factor (BSAF) values of coral samples revealed that species examined in the present study are reliable biomonitors for Cd, Ni, Pb, and Zn contamination. Principal component analysis (PCA) and Pearson's correlation coefficient (PCC) suggested that Pb, Ni, and Zn can be considered the main pollutants of the Persian Gulf which originated mainly from petroleum industries. Furthermore, the metal uptake rates of coral samples seemed to be dependent on bioabsorption pathways and coral species. Overall, the present work constitutes a good basis for further studies on trace metal fractionation, risk assessment, and source apportionment in the Persian Gulf, which could contribute to more effective decisions for reducing the anthropogenic trace metal pollution.

Microplastic ingestion in important commercial fish in the southern Caspian Sea.

The ubiquitous distribution of microplastics (MPs) across marine habitats has led to an increased investigation of their potential impacts on the marine food chain and consequent human exposure. The two fish species Chelon aurata and Rutilus kutum that account for over 50% of the total catch in the Caspian Sea were assessed relative to the presence of MPs in their digestive system. Samples were collected from 6 stations in the southern Caspian Sea from February to March 2017. MPs were reported to be present in 67.56% of the 111 individuals analyzed, with an average concentration of 2.29 MP/Fish; the majority were fibers (≈50%) and fragments (≈30%). The baseline data have become more relevant since the population in the region has consumed the whole fish. The presence and prevalence of MP in the two commercially important fish in the Caspian Sea warranted a comprehensive assessment in the water column, marine sediments, and fish from the different trophic levels as over 130 rivers drain into the water body.

Standardized protocols for microplastics determinations in environmental samples from the Gulf and marginal seas.

Microplastics are a group of ubiquitous persistent pollutants that have rapidly attracted much attention from the scientific community as well as the general public due to the growing awareness of the environmental risks they pose. However, due to limitations and variations in sampling, analytical measurement methods, and the different units used for reporting data, reliable comparisons between studies in the Gulf region and internationally are not straightforward. This study proposes standardized protocols for marine sediment, seawater, marine biota and aerosol (1) sampling, (2) sample processing, (3) sample identification and (4) reporting units to be used. An attempt has been made to highlight the limitations of the widely employed strategies for sampling microplastics in seawater, where a large portion of the microplastics is not sampled due to the mesh sizes used. The issues with the processing of biota samples and aerosols are likewise addressed, and recommendations are also made for standardization of units for reporting microplastic quantification. Protocols for collection of bottom sediments and aerosols are also proposed. These are the environmental matrixes for which there are no harmonized protocols in the Gulf region; hence if a standardized approach is adopted, it will enable and improve comparisons between the studies within this region and can be useful for similar studies in other marine areas as well.

Small microplastic particles (S-MPPs) in sediments of mangrove ecosystem on the northern coast of the Persian Gulf.

We present a study of small microplastic particles (S-MPPs) in the sediments of mangrove ecosystem of Khor-e- Khoran, a Ramsar site in Iran. The spatial distribution of S-MPPs (<1 mm) in mangrove surface sediments were investigated, which provided new insights into the detection and composition of S-MPPs in the study area. S-MPPs were extracted via the air-induced overflow (AIO) extraction procedure, and then they were counted and categorized according to the particle shape, color and size. The mean number of S-MPPs at the five sampling sites ranged from 19.5 to 34.5 particles per kg dry sediment in Bandar Gelkan and Bandar Lengeh, respectively. In general, microfibres followed by fragments were the most common type of S-MPPs isolated in each site (>56% and ~35%, respectively). Sewage discharge is probably the main source of extracted fibres in almost all the sites. The observed S-MPPs were classified into two size groups (10-300 µm and 300-1000 µm). The majority of S-MPPs fell into the smallest size group which accounted for 70-97% of the total S-MPPs. Fourier transform infrared (FTIR) analysis of some subsamples showed that polyethylene (PE) was the most common recovered polymer. Some non-plastic particles were also isolated from plastic-like particles of suspected S-MPPs in the mangrove sediments using a Scanning Electron Microscope (FE-SEM). This study provided the first evidence of S-MPPs contamination in the mangroves of the Iranian coast of the Persian Gulf. Long-term studies are required to understand, monitor and prevent further microplastics pollution in the region.

Microplastics contamination in molluscs from the northern part of the Persian Gulf.

Microplastics (MPs) are well-known emerging contaminants in the marine environment. A key route by which MPs can directly affect marine life is through ingestion. The objective of the present study was to evaluate the occurrence of MPs in marine life and seafood for human consumption in the Persian Gulf. We conducted a whole body analysis of MP (between 10 and 5000 µm in diameter) abundance in five species of molluscs with different feeding strategies, including both gastropods and bivalves from the littoral zone of the Iranian coast of the Persian Gulf. The mean number of total encountered MPs in all species ranged from 0.2 to 21.0 particles per g of soft tissue (wet weight) and from 3.7 to 17.7 particles per individual. Overall, microfibres followed by fragments were the most common type of MP isolated in each species (respectively > 50% and ≈26%). Film (≈14%) and pellets (≈2%) were less commonly observed. The observed MPs were classified into three size groups (ca. 10-25 µm, 25-250 µm and 250-5000 µm), and 37-58% of MPs fell into the smallest size group. Fourier transform infrared (FT-IR) analysis confirmed the presence of polyethylene (PE), polyethylene terephthalate (PET), and nylon (PA). Our results indicated that molluscan shellfish from the Persian Gulf contain MPs, with higher concentrations in a predatory species, suggesting trophic transfer of MPs in the food web. The consumption of edible species may be a source of human microplastic intake. We compared our results with those previously reported for other regions of the world and identified the need for further studies in the Persian Gulf.

Phytomanagement of trace metals in mangrove sediments of Hormozgan, Iran, using gray mangrove (Avicennia marina).

Trace elements (Zn, Cu, Pb, and Cd) in root and leaf tissues of the gray mangrove (Avicennia marina) and in corresponding sediment samples were studied. Samples were taken from the inflow/outflow points in two distinct habitats, i.e., the Hara Protected Area and the Azini Bay, of Hormozgan Province in south Iran. Heavy metal concentrations (µg g-1 of dry weight) in the sediments of the Hara Protected Area ranged from 16.0 to 68.0 for Pb, 15.0 to 52.0 for Zn, 9.0 to 27.0 for Cu, and 1.0 to 3.3 for Cd. In the Azini Bay, these concentrations ranged from 7.1 to 27.5 for Pb, 17.1 to 55.9 for Zn, 12.1 to 37.9 for Cu, and 0.2 to 2.3 for Cd. The accumulation trend of heavy metal concentrations in the roots of A. marina was in the order Pb (16.1) > Zn (15.8) > Cu (9.3) > Cd (1.3) µg g-1 of dry weight in the Hara Protected Area and in the order Zn (13.7) > Cu (9.4) > Pb (5.5) > Cd (0.6) µg g-1 of dry weight in the Azini Bay. The value of translocation factor (TLF) was smaller than 1 in both regions. It was estimated from 0.44 to 0.62 in the Hara Protected Area and from 0.51 to 1.01 in the Azini Bay. The enrichment coefficient for root (ECR) varied from 0.32 to 0.93 in the Hara Protected Area and from 0.32 to 0.51 in the Azini Bay. The ratio of heavy metals in leaves/sediments (ECL) also varied from 0.01 to 0.67 in the Hara Protected Area and from 0.01 to 0.47 in the Azini Bay. The enrichment coefficient for leaf (ECL) was always lower than ECR in both regions. Based on the above findings, A. marina can be regarded as an excluder for the heavy metals examined in this study, given its low efficiency in translocating and accumulating the heavy metals in the shoots. Apart from serving as a baseline for the study area, findings could be useful for mitigating heavy metal contamination in these sensitive ecosystems through possible phytomanagement using gray mangrove.

RETRACTED: Ecological risk assessment of coastal ecosystems: The case of mangrove forests in Hormozgan Province, Iran.

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of at the request of the Editors-in-Chief. After a thorough investigation, the Editors have concluded that the acceptance of this article was partly based upon the positive advice of three illegitimate reviewer reports. The reports were submitted from email accounts which were provided by the corresponding author C.A. Damalas as suggested reviewers during the submission of the article. Although purportedly real reviewer accounts, the Editors have concluded that these were not of appropriate, independent reviewers. This manipulation of the peer-review process represents a clear violation of the fundamentals of peer review, our publishing policies, and publishing ethics standards. Apologies are offered to the reviewers whose identity was assumed and to the readers of the journal that this deception was not detected during the submission process.

Plastic debris and microplastics along the beaches of the Strait of Hormuz, Persian Gulf.

Currently little is known about the prevalence of plastics and microplastics (MPs) in the Persian Gulf. Five sampling stations were selected along the Strait of Hormuz (Iran) that exhibited different levels of industrialization and urbanization, and included a marine protected area. Debris was observed and sediments were collected for MPs extraction via fluidization/floatation methodology. The order of MP abundance (par/kg) generally reflected the level of anthropogenic activity: Bostanu (1258±291)>Gorsozan (122±23)>Khor-e-Yekshabeh (26±6)>Suru (14±4)>Khor-e-Azini (2±1). Across all sites fibers dominated (83%, 11% film, 6% fragments). FT-IR analysis showed polyethylene (PE), nylon, and PET (polyethylene terephthalate) were the commonly recovered polymers. Likely sources include beach debris, discarded fishing gear, and urban and industrial outflows that contain fibers from clothes. This study provides a 'snapshot' of MP pollution and longitudinal studies are required to fully understand plastic contamination in the region.

The occurrence of microplastic contamination in littoral sediments of the Persian Gulf, Iran.

Microplastics (MPs; <5 mm) in aquatic environments are an emerging contaminant of concern due to their possible ecological and biological consequences. This study addresses that MP quantification and morphology to assess the abundance, distribution, and polymer types in littoral surface sediments of the Persian Gulf were performed. A two-step method, with precautions taken to avoid possible airborne contamination, was applied to extract MPs from sediments collected at five sites during low tide. MPs were found in 80% of the samples. Across all sites, fiber particles were the most dominate shape (88%), followed by films (11.2%) and fragments (0.8%). There were significant differences in MP particle concentration between sampling sites (p value <0.05). The sediments with the highest numbers of MPs were from sites in the vicinity of highly populated centers and municipal effluent discharges. FTIR analysis showed that polyethylene (PE), nylon, and polyethylene terephthalate (PET) were the most abundant polymer types. More than half of the observed MPs (56%) were in the size category of 1-4.7 mm length, with the remaining particles (44%) being in the size range of 10 µm to <1 mm. Compared to literature data from other regions, intertidal sediments in the Persian Gulf cannot be characterized as a hot spot for MP pollution. The present study could, however, provide useful background information for further investigations and management policies to understand the sources, transport, and potential effects on marine life in the Persian Gulf.

Potential human health risk assessment of trace metals via the consumption of marine fish in Persian Gulf.

This study was carried out to evaluate the concentration of trace metals (Cd, Cu, Ni, Pb and Zn) in the muscle of four fish species from the Persian Gulf. Trace metals were analyzed using atomic absorption spectroscopy and consumption rates advisory for minimizing chronic systemic effects in children and adults were estimated. The metals concentrations in analyzed fish samples were lower than legal limits. Cadmium target hazard quotient values suggested that the threshold to avoid the potential risk for children health is an exposure level lower than 3 meals per week. Hazard index values based on four metals (not including Pb) for the child age class were higher than those of the adult age class, suggesting that children may suffer from a higher health risk. This study provides information about the consumption limits of certain metals, in particular Cd, necessary for minimizing potential health risks resulting from human consumption.