Oxidative balance score and oxidative stress biomarkers in a study of Whites, African Americans, and African immigrants.

CONTEXT: Oxidative balance score (OBS) is a composite measure of multiple pro- and antioxidant exposures. OBJECTIVE: To investigate associations of OBS with F2-isoprostanes (FIP), mitochondrial DNA copy number (mtDNA), and fluorescent oxidative products (FOP), and assess inter-relationships among the biomarkers. METHODS: In a cross-sectional study, associations of a thirteen-component OBS with biomarker levels were assessed using multivariable regression models. RESULTS: Association of OBS with FIP, but not with FOP, was in the hypothesized direction. The results for mtDNA were unstable and analysis-dependent. The three biomarkers were not inter-correlated. CONCLUSIONS: Different biomarkers of oxidative stress may reflect different biological processes.

Rationale and design of a study using a standardized locally procured macronutrient supplement as adjunctive therapy to HIV treatment in Kenya.

Poor nutritional status at initiation of antiretroviral therapy (ART) is predictive of mortality. Decreased dietary intake is a major determinant of weight loss in HIV. Despite a biological rationale to treat undernutrition in adults receiving ART, few studies have provided data on feasibility, safety, effectiveness, and sustainability of specific macronutrient supplements with HIV treatment in adults, especially supplements such as a food basket, a supplement approach seldom evaluated in spite of its wide use. We present the rationale and design for a study of a locally procured macronutrient supplement given to HIV-infected patients initiating ART with a body mass index (BMI) ≤20.0 kg/m(2). The objective was to determine feasibility of procurement, distribution, safety and to obtain preliminary effectiveness data for a locally procured supplement. The design was a comparative study for 200 adult participants at two Kenya government-supported clinics. The primary outcome was BMI at 24 weeks. Supplement duration was 24 weeks, total follow-up was 48 weeks, and the study included a comparison site. Novel aspects of this study include use of a standardized macronutrient supplement to protect the participant against household food sharing, and a complementary micronutrient supplement. Comprehensive data collected included dietary intake, HIV-related quality-of-life, food security, neuropsychiatric assessments, laboratory studies, and household geomapping. Assessments were made at baseline, at 24 weeks, and at 48 weeks post-ART initiation. Challenges included establishing a partnership with local millers, distribution from the HIV clinic, food safety, and tracking of participants. These findings will help inform nutrition support programming in Kenya and similar settings, and provide needed data regarding use of macronutrient supplements as an adjunctive intervention with ART.

Nutritional status of under-five children in HIV-affected households in western Kenya.

BACKGROUND: Households affected by HIV/AIDS are at an increased risk for food insecurity and malnutrition. Poor nutrition contributes to more than a third of all deaths associated with infectious diseases among children under 5 years of age in developing countries. With increased household food insecurity, and a greater disease burden associated with HIV/AIDS, the growth of children under five could be impacted, resulting in increased malnutrition for this vulnerable group. OBJECTIVE: To determine whether there is an association between the type of household (HIV-affected compared with HIV-unaffected) and the nutritional status of children under 5 years of age residing in these households. METHODS: The study was set in a Millennium Village Project site in western Kenya and used a cross-sectional design to compare the stunting, wasting, and underweight status among 102 and 99 under-five children living in HIV-affected and -unaffected households, respectively. Height-for-age, weight-for-age, and weight-for-age z-scores were calculated based on the World Health Organization growth standards and compared. Proportions, means, and standard deviations were used to describe the data. The data were analyzed with the use of the chi-square test for comparison of proportions and the independent t-test for comparison of means. RESULTS: Children in HIV-affected households had a significantly higher degree of stunting (height-for-age < -2 SD) than children in unaffected households (25.5% vs. 9.1%, p = .002). The degree of wasting and underweight did not differ significantly between HIV-affected and -unaffected households. CONCLUSIONS: Residing in HIV-affected households is associated with stunting in children under 5 years of age.

Self-reported and measured height and weight: impact on racial/ethnic differences in hypertension.

OBJECTIVE: We compare estimability of obesity from self-reported and measured height and weight in White, Black, and Hispanic Americans. We also sought to determine the effect of using self-reported and measured height and weight in determining the association of obesity with risk of hypertension in these population groups. METHODS: The 1999-2000 National Health and Nutrition Examination Survey (NHANES 1999-2000) participants' (n=4789) self-reported and measured height and weight were used for this study. Logistic regression adjusted for age, blood glucose level, total cholesterol level, smoking status, and exercise status to compare the association of obesity estimated from self-reported and measured height and weight on the prevalence odds of hypertension. RESULTS: Men tended to overestimate height and weight, and women tended to overestimate height and underestimate weight. Using self-reported values diminished the prevalence of obesity and odds of hypertension, and this effect related to ethnicity and sex. In men, self-report decreased the prevalence of hypertension by 9.1%, 11.8%, and 26.6% in Whites, Blacks, and Hispanics, respectively. The analogous values in women were 11.1%, 22.7%, and 7.7%. CONCLUSION: Public health researchers and practitioners who use self-reported height and weight should be aware of the potential for error when using self-reported values to estimate obesity so that they may make better decisions regarding obesity screening and prevention.

Perceptions of factors influencing healthful food consumption behavior in the Lower Mississippi Delta: focus group findings.

OBJECTIVE: To identify perceptions of Lower Mississippi Delta (LMD) residents regarding factors that influence a change in healthful food consumption behavior to assist in planning sustainable nutrition interventions in the LMD. DESIGN: Nine focus groups were conducted with LMD residents in 9 counties in Arkansas, Louisiana, and Mississippi. One focus group was held in each county on the topical area of behavioral change. SETTING: Nine counties in Arkansas, Louisiana, and Mississippi. PARTICIPANTS: The study population included 91 persons, 85 females and 6 males (18-60+ years of age), of whom 71 were African Americans, 17 were Caucasians, and 3 were Hispanics, who participated in the focus group discussions. ANALYSIS: Data analyses were completed by general and specific content coding. Data were reviewed for emerging themes for each topic. The Social Cognitive Theory served as the framework for understanding the determinants of a change in healthful food consumption behavior. RESULTS: The study showed considerable variability in perceptions that are influenced by both personal and external factors. These factors include health concerns, family influence, and need for and availability of nutrition information. Participants were interested in learning about healthful eating, food preparation skills, and portion control. CONCLUSIONS: Focus groups in the LMD identified many important themes relevant to the development of nutrition interventions in these communities. These data will be used to guide the community-based participatory interventions that will be developed and implemented in the LMD. The findings could be applicable to other researchers designing interventions for similar populations.

Community--academia partnerships to promote nutrition in the lower Mississippi delta: community members' perceptions of effectiveness, barriers, and factors related to success.

This study examined the perceptions of community members' engaged in community-academia partnerships involved in developing nutrition interventions in three communities in the Lower Mississippi Delta. Perceptions on effectiveness of the partnerships were investigated. Six focus group interviews were conducted, with 33 participants that included 27 females and 6 males. The data were analyzed by content coding. Emerging themes were identified and related to accomplishments, barriers to success, and factors related to success of the partnerships. Accomplishments included the establishment of active committees, positive changes in health behavior related to food choices, and participation in community events. Barriers to success included the slow pace of intervention implementation, difficulties with understanding the role of the community in the participatory research process, the decision-making processes, and project name recognition. Factors related to success were tangible benefits to the community, participation that was representative, simplification of the research process, and the decision-making processes.

Oxidative stress, oxidative balance score, and hypertension among a racially diverse population.

Hypertension is a risk factor for several vascular diseases. Evidence suggests that oxidative stress (OS) plays a significant role in its pathophysiology. Human studies have shown inconsistent results, varying based on the OS biomarker and study population. In a racially diverse population, examine the association between: (1) blood pressure or hypertension and four markers of OS and (2) blood pressure or hypertension and oxidative balance score (OBS). Using data (n = 317) from the cross-sectional study on race, stress, and hypertension, an OBS was constructed from various measures of pro-oxidant and antioxidant exposures. OS was assessed by four biomarkers: fluorescence oxidative products, F2-isoprostanes, mitochondrial DNA copy number, and gamma tocopherol. Multivariate linear and logistic regression analyses were used to estimate the associations of interest. None of the adjusted associations between hypertension and OS markers was statistically significant. OBS was inversely associated with hypertension after adjusting for study covariates. Persons with higher OBS have lower odds of having hypertension; however, the evidence on the relationship between OS markers and blood pressure remains unconvincing.

Perceptions of food-insecure HIV-positive adults participating in a food supplementation program in central Kenya.

Malnutrition coexists with HIV in sub-Saharan Africa. Food supplementation is recommended for food-insecure, HIV-positive individuals. This study was part of a larger six-month food supplementation program for adults initiating antiretroviral therapy (ART) in central Kenya. We conducted 10 focus group interviews with program participants to examine the perceptions of participants regarding the food supplementation program. Focus group transcripts were analyzed for themes and six were identified. These were perception of food insecurity and the health of the participants, the benefits of participating, use of the food, coping strategies after the program ended, suggestions for improving the program, and sustainability of the benefits. Participants perceived that the food improved their health and ART adherence, and reduced stigma. The improvements were not always sustained. Sharing with people beyond the immediate family was very common, depleting the food available to the participants. Interventions with sustainable effects for food-insecure, HIV-positive individuals and their families are needed.

Compliance and acceptability of maintaining a 6-month pedometer diary in a rural, African American community-based walking intervention.

BACKGROUND: Limited research has been done on the compliance and acceptability of maintaining pedometer diaries for an extensive time frame in community-based interventions targeting minority populations. METHODS: Community "coaches" led participants in a 6-month community-based walking intervention that included wearing pedometers and maintaining pedometer diaries for the study duration. Descriptive statistics and ANOVA tests were used to evaluate compliance rates for maintaining diaries and daily step counts. After the intervention, focus groups were used to explore opinions regarding pedometers. Audiotapes were transcribed and evaluated using systematic content analysis. RESULTS: The 8 coaches and 75 enrolled walking participants were primarily African American (98%) women (94%). Overall, the group (N = 83) submitted 85% of all possible pedometer diaries and recorded 73% of all possible daily step counts. Walking-group members were significantly (P < .01) more compliant if their coach was also compliant. Identified benefits of wearing pedometers and maintaining diaries outnumbered the barriers. Participants were enthusiastic about wearing the pedometers and indicated that the weekly diaries provided a source of motivation. CONCLUSIONS: This research suggests pedometer diaries are a viable intervention tool and research method for community-based physical activity interventions targeting African Americans and highlights the need for social support to promote pedometer diary compliance.

Conducting needs assessment using the comprehensive participatory planning and evaluation model to develop nutrition and physical activity interventions in a rural community in the Mississippi delta.

BACKGROUND: Members of a Lower Mississippi Delta community and university partners used the Comprehensive Participatory Planning and Evaluation (CPPE) model to assess nutrition and health problems and develop a menu of interventions. OBJECTIVES: We sought to identify and prioritize nutrition and physical activity problems in the community and to identify interventions to address the problems. METHODS: Community members and university partners used the CPPE process to identify and prioritize nutrition and physical activity problems. The participants developed causal models to break down the identified problems to their root causes. They then developed a menu of interventions and criteria to rank the interventions. RESULTS: The identified problems were intake of unhealthy foods, lack of nutrition education, and lack of adequate physical activity. The menu of interventions consisted of seven objectives to address poor nutrition and physical activity as well as a total of 19 interventions to meet these objectives. CONCLUSION: Directly involving community members in identifying health problems and solutions results in the development of interventions that are likely to have greater acceptability with the community.