RESUMO
OBJECTIVE: The aim of this study was to investigate the cognitive effects of unilateral directional versus ring subthalamic nucleus deep brain stimulation (STN DBS) in patients with advanced Parkinson's disease. METHODS: We examined 31 participants who underwent unilateral STN DBS (left n = 17; right n = 14) as part of an National Institutes of Health (NIH)-sponsored randomized, double-blind, crossover study contrasting directional versus ring stimulation. All participants received unilateral DBS implants in the hemisphere more severely affected by motor parkinsonism. Measures of cognition included verbal fluency, auditory-verbal memory, and response inhibition. We used mixed linear models to contrast the effects of directional versus ring stimulation and implant hemisphere on longitudinal cognitive function. RESULTS: Crossover analyses showed no evidence for group-level changes in cognitive performance related to directional versus ring stimulation. Implant hemisphere, however, impacted cognition in several ways. Left STN participants had lower baseline verbal fluency than patients with right implants (t [20.66 = -2.50, p = 0.02]). Verbal fluency declined after left (p = 0.013) but increased after right STN DBS (p < 0.001), and response inhibition was faster following right STN DBS (p = 0.031). Regardless of hemisphere, delayed recall declined modestly over time versus baseline (p = 0.001), and immediate recall was unchanged. INTERPRETATION: Directional versus ring STN DBS did not differentially affect cognition. Similar to prior bilateral DBS studies, unilateral left stimulation worsened verbal fluency performance. In contrast, unilateral right STN surgery increased performance on verbal fluency and response inhibition tasks. Our findings raise the hypothesis that unilateral right STN DBS in selected patients with predominant right brain motor parkinsonism could mitigate declines in verbal fluency associated with the bilateral intervention. ANN NEUROL 2024;95:1205-1219.
Assuntos
Cognição , Estudos Cross-Over , Estimulação Encefálica Profunda , Doença de Parkinson , Núcleo Subtalâmico , Humanos , Estimulação Encefálica Profunda/efeitos adversos , Estimulação Encefálica Profunda/métodos , Doença de Parkinson/terapia , Doença de Parkinson/fisiopatologia , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Método Duplo-Cego , Cognição/fisiologiaRESUMO
Although sentences unfold sequentially, one word at a time, most linguistic theories propose that their underlying syntactic structure involves a tree of nested phrases rather than a linear sequence of words. Whether and how the brain builds such structures, however, remains largely unknown. Here, we used human intracranial recordings and visual word-by-word presentation of sentences and word lists to investigate how left-hemispheric brain activity varies during the formation of phrase structures. In a broad set of language-related areas, comprising multiple superior temporal and inferior frontal sites, high-gamma power increased with each successive word in a sentence but decreased suddenly whenever words could be merged into a phrase. Regression analyses showed that each additional word or multiword phrase contributed a similar amount of additional brain activity, providing evidence for a merge operation that applies equally to linguistic objects of arbitrary complexity. More superficial models of language, based solely on sequential transition probability over lexical and syntactic categories, only captured activity in the posterior middle temporal gyrus. Formal model comparison indicated that the model of multiword phrase construction provided a better fit than probability-based models at most sites in superior temporal and inferior frontal cortices. Activity in those regions was consistent with a neural implementation of a bottom-up or left-corner parser of the incoming language stream. Our results provide initial intracranial evidence for the neurophysiological reality of the merge operation postulated by linguists and suggest that the brain compresses syntactically well-formed sequences of words into a hierarchy of nested phrases.
Assuntos
Encéfalo/fisiologia , Lobo Frontal/fisiologia , Modelos Neurológicos , Fala/fisiologia , Lobo Temporal/fisiologia , Feminino , Humanos , MasculinoRESUMO
Four patients with primary progressive aphasia displayed a greater deficit in understanding words they heard than words they read, and a further deficiency in naming objects orally rather than in writing. All four had frontotemporal lobar degeneration-transactive response DNA binding protein Type A neuropathology, three determined postmortem and one surmised on the basis of granulin gene (GRN) mutation. These features of language impairment are not characteristic of any currently recognized primary progressive aphasia variant. They can be operationalized as manifestations of dysfunction centered on a putative auditory word-form area located in the superior temporal gyrus of the left hemisphere. The small size of our sample makes the conclusions related to underlying pathology and auditory word-form area dysfunction tentative. Nonetheless, a deeper assessment of such patients may clarify the nature of pathways that link modality-specific word-form information to the associations that mediate their recognition as concepts. From a practical point of view, the identification of these features in patients with primary progressive aphasia should help in the design of therapeutic interventions where written communication modalities are promoted to circumvent some of the oral communication deficits.
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Afasia Primária Progressiva/fisiopatologia , Percepção de Forma/fisiologia , Degeneração Lobar Frontotemporal/patologia , Percepção da Fala/fisiologia , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Lobo Temporal/patologiaRESUMO
BACKGROUND AND AIMS: Eosinophil predominant mucosal inflammation is central to the diagnosis and activity assessment of eosinophilic esophagitis (EoE). Esophageal mural remodeling is an important consequence of EoE that is responsible for adverse events of dysphagia, food impaction, and esophageal stenosis. The aim of this study was to compare upper endoscopy (EGD) with barium upper GI study (UGI) for the detection of esophageal inflammation and remodeling in adults with EoE. METHODS: A retrospective review on a single-center database of adults with confirmed EoE identified those with EGD and UGI performed within 6 months of each another. Studies were reviewed for mucosal inflammatory and remodeling abnormalities. RESULTS: Seventy patients were included. Initial UGI results were consistent with EoE in 10% and suggestive of EoE in 39%. Review of UGI by a senior GI radiologist increased detection of changes consistent with EoE (34%). EGD identified characteristic abnormalities in 93%, which was significantly greater than UGI (67%). Inflammatory features were more frequently appreciated on EGD (74%) compared with UGI (21%). There was no significant difference in fibrostenotic changes observed on EGD (84%) versus UGI (73%). CONCLUSIONS: EGD and UGI have similar sensitivity for identifying the remodeling consequences of EoE; however, inflammatory features are better assessed on EGD. Inadequate sensitivity of UGI for composite features of EoE limits its capabilities as a diagnostic test, although radiologists' awareness significantly increases the diagnostic yield of UGI. UGI and EGD may identify fibrostenotic changes unappreciated by its counterpart and thus provide complementary information in select patients.
Assuntos
Esofagite Eosinofílica/diagnóstico por imagem , Estenose Esofágica/diagnóstico por imagem , Esofagoscopia , Esôfago/diagnóstico por imagem , Esôfago/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Sulfato de Bário , Meios de Contraste , Transtornos de Deglutição/etiologia , Esofagite Eosinofílica/complicações , Estenose Esofágica/etiologia , Feminino , Fibrose , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Adulto JovemRESUMO
Modern neurophysiological experiments frequently involve multiple channels separated by very small distances. A unique methodological concern for multiple-electrode experiments is that of capacitive coupling (cross-talk) between channels. Yet the nature of the cross-talk recording circuit is not well known in the field, and the extent to which it practically affects neurophysiology experiments has never been fully investigated. Here we describe a simple electrical circuit model of simultaneous recording and stimulation with two or more channels and experimentally verify the model using ex vivo brain slice and in vivo whole-brain preparations. In agreement with the model, we find that cross-talk amplitudes increase nearly linearly with the impedance of a recording electrode and are larger for higher frequencies. We demonstrate cross-talk contamination of action potential waveforms from intracellular to extracellular channels, which is observable in part because of the different orders of magnitude between the channels. This contamination is electrode impedance-dependent and matches predictions from the model. We use recently published parameters to simulate cross-talk in high-density multichannel extracellular recordings. Cross-talk effectively spatially smooths current source density (CSD) estimates in these recordings and induces artefactual phase shifts where underlying voltage gradients occur; however, these effects are modest. We show that the effects of cross-talk are unlikely to affect most conclusions inferred from neurophysiology experiments when both originating and receiving electrode record signals of similar magnitudes. We discuss other types of experiments and analyses that may be susceptible to cross-talk, techniques for detecting and experimentally reducing cross-talk, and implications for high-density probe design.NEW & NOTEWORTHY We develop and experimentally verify an electrical circuit model describing cross-talk that necessarily occurs between two channels. Recorded cross-talk increased with electrode impedance and signal frequency. We recorded cross-talk contamination of spike waveforms from intracellular to extracellular channels. We simulated high-density multichannel extracellular recordings and demonstrate spatial smoothing and phase shifts that cross-talk enacts on CSD measurements. However, when channels record similar-magnitude signals, effects are modest and unlikely to affect most conclusions.
Assuntos
Encéfalo/fisiologia , Microeletrodos , Modelos Neurológicos , Neurônios/fisiologia , Potenciais de Ação , Animais , Simulação por Computador , Impedância Elétrica , Masculino , Técnicas de Patch-Clamp , Ratos , Técnicas de Cultura de TecidosRESUMO
We investigated the chronometry of neural processes in frontal eye fields of macaques performing double-step saccade visual search in which a conspicuous target changes location in the array on a random fraction of trials. Durations of computational processes producing a saccade to original and final target locations (GO1 and GO2, respectively) are derived from response times (RT) on different types of trials. In these data, GO2 tended to be faster than GO1, demonstrating that inhibition of the initial saccade did not delay production of the compensated saccade. Here, we measured the dynamics of visual, visuomovement, and movement neuron activity in relation to these processes by examining trials when neurons instantiated either process. First, we verified that saccades were initiated when the discharge rate of movement neurons reached a threshold that was invariant across RT and trial type. Second, the time when visual and visuomovement neurons selected the target and when movement neuron activity began to accumulate were not significantly different across trial type. Third, the interval from the beginning of accumulation to threshold of movement-related activity was significantly shorter when instantiating the GO2 relative to the GO1 process. Differences observed between monkeys are discussed. Fourth, random variation of RT was accounted for to some extent by random variation in both the onset and duration of selective activity of each neuron type but mostly by variation of movement neuron accumulation duration. These findings offer new insights into the sources of control of target selection and saccade production in dynamic environments.
Assuntos
Potenciais Evocados Visuais , Tempo de Reação , Movimentos Sacádicos , Campos Visuais , Animais , Lobo Frontal/citologia , Lobo Frontal/fisiologia , Macaca mulatta , Macaca radiata , Neurônios/fisiologiaRESUMO
Interpretations of local field potentials (LFPs) are typically shaped on an assumption that the brain is a homogenous conductive milieu. However, microscale inhomogeneities including cell bodies, dendritic structures, axonal fiber bundles and blood vessels are unequivocally present and have different conductivities and permittivities than brain extracellular fluid. To determine the extent to which these obstructions affect electrical signal propagation on a microscale, we delivered electrical stimuli intracellularly to individual cells while simultaneously recording the extracellular potentials at different locations in a rat brain slice. As compared with relatively unobstructed paths, signals were attenuated across frequencies when fiber bundles were in between the stimulated cell and the extracellular electrode. Across group of cell bodies, signals were attenuated at low frequencies, but facilitated at high frequencies. These results show that LFPs do not reflect a democratic representation of neuronal contributions, as certain neurons may contribute to the LFP more than others based on the local extracellular environment surrounding them.
Assuntos
Encéfalo/fisiologia , Fenômenos Eletrofisiológicos/fisiologia , Animais , Axônios/fisiologia , Vasos Sanguíneos/fisiologia , Encéfalo/citologia , Dendritos/fisiologia , Estimulação Elétrica , Espaço Extracelular/fisiologia , Feminino , Processamento de Imagem Assistida por Computador , Masculino , Potenciais da Membrana/fisiologia , Fibras Nervosas/fisiologia , Neuroglia/fisiologia , Técnicas de Patch-Clamp , RatosRESUMO
We often face alternatives that we are free to choose between. Planning movements to select an alternative involves several areas in frontal and parietal cortex that are anatomically connected into long-range circuits. These areas must coordinate their activity to select a common movement goal, but how neural circuits make decisions remains poorly understood. Here we simultaneously record from the dorsal premotor area (PMd) in frontal cortex and the parietal reach region (PRR) in parietal cortex to investigate neural circuit mechanisms for decision making. We find that correlations in spike and local field potential (LFP) activity between these areas are greater when monkeys are freely making choices than when they are following instructions. We propose that a decision circuit featuring a sub-population of cells in frontal and parietal cortex may exchange information to coordinate activity between these areas. Cells participating in this decision circuit may influence movement choices by providing a common bias to the selection of movement goals.
Assuntos
Comportamento de Escolha/fisiologia , Lobo Frontal/fisiologia , Macaca mulatta/fisiologia , Vias Neurais/fisiologia , Lobo Parietal/fisiologia , Potenciais de Ação/fisiologia , Animais , Fixação Ocular/fisiologia , Masculino , Neurônios/metabolismo , Estimulação Luminosa , Probabilidade , Curva ROC , Recompensa , Movimentos Sacádicos/fisiologiaRESUMO
Objective: To investigate hemispheric effects of directional versus ring subthalamic nucleus (STN) deep brain stimulation (DBS) surgery on cognitive function in patients with advanced Parkinson's disease (PD). Methods: We examined 31 PD patients (Left STN n = 17; Right STN n = 14) who underwent unilateral subthalamic nucleus (STN) DBS as part of a NIH-sponsored randomized, cross-over, double-blind (ring vs directional) clinical trial. Outcome measures were tests of verbal fluency, auditory-verbal memory, and response inhibition. First, all participants were pooled together to study the effects of directional versus ring stimulation. Then, we stratified the groups by surgery hemisphere and studied the longitudinal changes in cognition post-unilateral STN DBS. Results: Relative to pre-DBS cognitive baseline performances, there were no group changes in cognition following unilateral DBS for either directional or ring stimulation. However, assessment of unilateral DBS by hemisphere revealed a different pattern. The left STN DBS group had lower verbal fluency than the right STN group (t(20.66 = -2.50, p = 0.02). Over a period of eight months post-DBS, verbal fluency declined in the left STN DBS group (p = 0.013) and improved in the right STN DBS group over time (p < .001). Similarly, response inhibition improved following right STN DBS (p = 0.031). Immediate recall did not significantly differ over time, nor was it affected by implant hemisphere, but delayed recall equivalently declined over time for both left and right STN DBS groups (left STN DBS p = 0.001, right STN DBS differ from left STN DBS p = 0.794). Conclusions: Directional and ring DBS did not differentially or adversely affect cognition over time. Regarding hemisphere effects, verbal fluency decline was observed in those who received left STN DBS, along with the left and right STN DBS declines in delayed memory. The left STN DBS verbal fluency decrement is consistent with prior bilateral DBS research, likely reflecting disruption of the basal-ganglia-thalamocortical network connecting STN and inferior frontal gyrus. Interestingly, we found an improvement in verbal fluency and response inhibition following right STN DBS. It is possible that unilateral STN DBS, particularly in the right hemisphere, may mitigate cognitive decline.
RESUMO
A question that still complicates interpretation of local field potentials (LFPs) is how electrode properties like impedance, size, and shape affect recorded LFPs. In addition, how any such effects should be considered when comparing LFP, electroencephalogram (EEG), or electrocorticogram (ECoG) data has not been clearly described. A generally accepted concrete physical model describes that an electrode records the spatial average of the voltage across its uninsulated tip, yet the effects of this spatial averaging on recorded coherence have never been modeled. Using simulations based on this physical model, we show here that for any effects to occur, a spatial voltage gradient on a scale smaller than an electrode's recording site must exist over the site's surface. When this occurs, larger electrodes on average report higher coherence between locations, with the effect continuously increasing as the voltage profile over the extent of the recording site is increasingly nonuniform. We quantitatively compared published coherence estimates of LFP, ECoG, and EEG data across a range of studies and found a possible modest effect of electrode size in published ECoG data only. We used the model to quantify the expected coherence for any electrode size in relation to any given spatial frequency of a voltage profile. From this and existing estimates of the spread of voltages underlying each of these data types, our simulations quantitatively agree with the published data and importantly suggest that LFP coherence will be independent of recording site size within the range of microelectrodes typically used for extracellular recordings.
Assuntos
Potenciais de Ação/fisiologia , Eletroencefalografia/instrumentação , Modelos Neurológicos , Eletrodos , Eletroencefalografia/métodosRESUMO
Spatial computations underlying the coordination of the hand and eye present formidable geometric challenges. One way for the nervous system to simplify these computations is to directly encode the relative position of the hand and the center of gaze. Neurons in the dorsal premotor cortex (PMd), which is critical for the guidance of arm-reaching movements, encode the relative position of the hand, gaze, and goal of reaching movements. This suggests that PMd can coordinate reaching movements with eye movements. Here, we examine saccade-related signals in PMd to determine whether they also point to a role for PMd in coordinating visual-motor behavior. We first compared the activity of a population of PMd neurons with a population of parietal reach region (PRR) neurons. During center-out reaching and saccade tasks, PMd neurons responded more strongly before saccades than PRR neurons, and PMd contained a larger proportion of exclusively saccade-tuned cells than PRR. During a saccade relative position-coding task, PMd neurons encoded saccade targets in a relative position code that depended on the relative position of gaze, the hand, and the goal of a saccadic eye movement. This relative position code for saccades is similar to the way that PMd neurons encode reach targets. We propose that eye movement and eye position signals in PMd do not drive eye movements, but rather provide spatial information that links the control of eye and arm movements to support coordinated visual-motor behavior.
Assuntos
Lobo Frontal/fisiologia , Neurônios/fisiologia , Movimentos Sacádicos/fisiologia , Potenciais de Ação , Animais , Braço/fisiologia , Objetivos , Macaca mulatta , Masculino , Microeletrodos , Modelos Neurológicos , Atividade Motora/fisiologia , Lobo Parietal/fisiologia , Desempenho Psicomotor/fisiologia , Fatores de TempoRESUMO
The purpose of this study was to test the hypothesis that exercise-induced cardiac adaptations would be attenuated by the free radical scavenger N-2-mercaptopropionyl glycine (MPG). Male Sprague-Dawley rats were divided into four groups (n = 9-13 per group) for 3-4 wk: sedentary (S), S+MPG (100 mg/kg ip daily), exercised on a treadmill (E) (60 min/day, 5 days/wk, at a speed of 20 m/min up a 6° grade in a 6°C room), or E+MPG given 10 min prior to exercise. Additional rats (n = 55) were used to determine acute exercise effects on myocardial redox state [nonprotein nonglutathione sulfhydryls (NPNGSH)] and PI3K/Akt signaling pathway activation. Compared with S, NPNGSH levels were 48% lower in E (P < 0.05) and unchanged in E+MPG (P > 0.05). MPG also attenuated exercise-induced activation of the signaling proteins Akt and S6. Hearts from the 4-wk groups were weighed, and cardiac function was evaluated using an isolated perfused working heart preparation. Similar increases (P < 0.05) in both exercised groups were observed for heart weight and heart weight-to-body weight ratio. Cardiac function improved in E vs. S, as indicated by greater (P < 0.05) external work performed (cardiac output × systolic pressure) and efficiency of external work (work/Vo(2)). MPG prevented these exercise-induced functional improvements. Skeletal muscle mitochondria content increased to similar levels in E and E+MPG. This study provides evidence that free radicals do not play an essential role in the development of exercise-induced cardiac hypertrophy; however, they appear to be involved in functional cardiac adaptations, which may be mediated through the PI3K/Akt pathway.
Assuntos
Antioxidantes/farmacologia , Coração/efeitos dos fármacos , Coração/fisiologia , Condicionamento Físico Animal/fisiologia , Tiopronina/farmacologia , Animais , Radicais Livres/metabolismo , Homeostase/fisiologia , Masculino , Modelos Animais , Oxirredução , Fosfatidilinositol 3-Quinases/fisiologia , Resistência Física/efeitos dos fármacos , Resistência Física/fisiologia , Proteínas Proto-Oncogênicas c-akt/fisiologia , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologiaRESUMO
Human activities put stress on our oceans and with a growing global population, the impact is increasing. Stressors rarely act in isolation, with the majority of marine areas being impacted by multiple, concurrent stressors. Marine spatial cumulative impact assessments attempt to estimate the collective impact of multiple stressors on marine environments. However, this is difficult given how stressors interact with one another, and the variable response of ecosystems. As a result, assumptions and generalisations are required when attempting to model cumulative impacts. One fundamental assumption of the most commonly applied, semi-quantitative cumulative impact assessment method is that a change in modelled cumulative impact is correlated with a change in ecosystem condition. However, this assumption has rarely been validated with empirical data. We tested this assumption using a case study of seagrass in a large, inverse estuary in South Australia (Spencer Gulf). We compared three different seagrass condition indices, based on survey data collected in the field, to scores from a spatial cumulative impact model for the study area. One condition index showed no relationship with cumulative impact, whilst the other two indices had very small, negative relationships with cumulative impact. These results suggest that one of the most commonly used methods for assessing cumulative impacts on marine systems is not robust enough to accurately reflect the effect of multiple stressors on seagrasses; possibly due to the number and generality of assumptions involved in the approach. Future methods should acknowledge the complex relationships between stressors, and the impact these relationships can have on ecosystems. This outcome highlights the need for greater evaluation of cumulative impact assessment outputs and the need for data-driven approaches. Our results are a caution for marine scientists and resource managers who may rely on spatial cumulative impact assessment outputs for informing policy and decision-making.
Assuntos
Ecossistema , Atividades Humanas , Estuários , Humanos , Oceanos e Mares , Austrália do SulRESUMO
When reaching to grasp an object, we often move our arm and orient our gaze together. How are these movements coordinated? To investigate this question, we studied neuronal activity in the dorsal premotor area (PMd) and the medial intraparietal area (area MIP) of two monkeys while systematically varying the starting position of the hand and eye during reaching. PMd neurons encoded the relative position of the target, hand, and eye. MIP neurons encoded target location with respect to the eye only. These results indicate that whereas MIP encodes target locations in an eye-centered reference frame, PMd uses a relative position code that specifies the differences in locations between all three variables. Such a relative position code may play an important role in coordinating hand and eye movements by computing their relative position.
Assuntos
Córtex Motor/fisiologia , Vias Neurais/fisiologia , Neurônios/fisiologia , Lobo Parietal/fisiologia , Desempenho Psicomotor/fisiologia , Percepção Espacial/fisiologia , Córtex Visual/fisiologia , Potenciais de Ação/fisiologia , Animais , Cognição/fisiologia , Olho , Mãos/inervação , Mãos/fisiologia , Macaca mulatta , Masculino , Córtex Motor/anatomia & histologia , Movimento/fisiologia , Vias Neurais/anatomia & histologia , Testes Neuropsicológicos , Orientação/fisiologia , Lobo Parietal/anatomia & histologia , Estimulação Luminosa , Retina/fisiologia , Córtex Visual/anatomia & histologia , Volição/fisiologiaRESUMO
OBJECTIVE: To explore atrophy-deficit correlations of word comprehension and repetition in temporoparietal cortices encompassing the Wernicke area, based on patients with primary progressive aphasia (PPA). METHODS: Cortical thickness in regions within and outside the classical Wernicke area, measured by FreeSurfer, was correlated with repetition and single word comprehension scores in 73 right-handed patients at mild to moderate stages of PPA. RESULTS: Atrophy in the Wernicke area was correlated with repetition (r = 0.42, p = 0.001) but not single word comprehension (r = -0.072, p = 0.553). Correlations with word comprehension were confined to more anterior parts of the temporal lobe, especially its anterior third (r = 0.60, p < 0.001). A single case with postmortem autopsy illustrated preservation of word comprehension but not repetition 6 months prior to death despite nearly 50% loss of cortical volume and severe neurofibrillary degeneration in core components of the Wernicke area. CONCLUSIONS: Temporoparietal cortices containing the Wernicke area are critical for language repetition. Contrary to the formulations of classic aphasiology, their role in word and sentence comprehension is ancillary rather than critical. Thus, the Wernicke area is not sufficient to sustain word comprehension if the anterior temporal lobe is damaged. Traditional models of the role of the Wernicke area in comprehension are based almost entirely on patients with cerebrovascular lesions. Such lesions also cause deep white matter destruction and acute network diaschisis, whereas progressive neurodegenerative diseases associated with PPA do not. Conceptualizations of the Wernicke area that appear to conflict, therefore, can be reconciled by considering the hodologic and physiologic differences of the underlying lesions.
Assuntos
Afasia Primária Progressiva/patologia , Afasia Primária Progressiva/fisiopatologia , Compreensão/fisiologia , Lobo Temporal/patologia , Vocabulário , Área de Wernicke/patologia , Idoso , Afasia Primária Progressiva/diagnóstico por imagem , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Testes de Linguagem , Estudos Longitudinais , Imageamento por Ressonância Magnética , MasculinoRESUMO
Interest in local field potentials (LFPs) and action potential shape has increased markedly. The present work describes distortions of these signals that occur for two reasons. First, the microelectrode recording circuit operates as a voltage divider producing frequency-dependent attenuation and phase shifts when electrode impedance is not negligible relative to amplifier input impedance. Because of the much higher electrode impedance at low frequencies, this occurred over frequency ranges of LFPs measured by neurophysiologists for one head-stage tested. Second, frequency-dependent phase shifts are induced by subsequent filters. Thus, we report these effects and the resulting amplitude envelope delays and distortion of waveforms recorded through a commercial data acquisition system and a range of tungsten microelectrodes. These distortions can be corrected, but must be accounted for when interpreting field potential and spike shape data.
Assuntos
Potenciais de Ação/fisiologia , Artefatos , Eletrônica/instrumentação , Eletrofisiologia/instrumentação , Neurofisiologia/instrumentação , Processamento de Sinais Assistido por Computador/instrumentação , Animais , Encéfalo/fisiologia , Impedância Elétrica , Eletrônica/métodos , Eletrofisiologia/métodos , Microeletrodos/normas , Neurônios/fisiologia , Neurofisiologia/métodosRESUMO
PURPOSE: This study determined the effects of a single session of heavy-resistance exercise on myosin heavy-chain (MHC) mRNA expression, along with the expression of the transcription factors Myo-D, myogenin, and Id-1. METHODS: Four male subjects participated in a control group (CON) and seven in a resistance-exercise group (REX). REX performed one resistance-exercise session employing three sets of 8-10 repetitions at 75-80% one-repetition maximum on the squat, leg press, and leg extension. Vastus lateralis biopsies were obtained pre, post, and at 6 h postexercise. RESULTS: In regard to CON, no significant differences were located for any criterion variable (P > 0.05). For REX, elevations of 38.19%, 45.61%, and 74.24% (P < 0.05), respectively, occurred at 6 h-post for Type I, IIa, and IIx MHC mRNA. Myo-D and myogenin mRNA were elevated 27.28% and 23.58% postexercise (P < 0.05), respectively, but no change was observed in Id-1 mRNA. Elevations at 6 h-post of 46.85% and 46.41% (P < 0.05), respectively, occurred for Myo-D and myogenin mRNA with no change in Id-1. Myo-D and myogenin protein increased 57.91% and 52.30%, respectively, postexercise and 317.56% and 254.08 at 6 h-post (P < 0.05), whereas no change was noted for Id-1. Myofibrillar protein was elevated 84.52% at 6 h-post (P < 0.05). Type I and IIa MHC mRNA at 6 h-post were correlated with myogenin mRNA and protein postexercise and 6 h-post, whereas Type IIx at 6 h-post was correlated with Myo-D mRNA and protein postexercise and 6 h-post (P < 0.05). CONCLUSIONS: These results indicate that the mRNA expression of all three MHC isoforms is up-regulated after a single session of heavy-resistance exercise and that Myo-D and myogenin seem to play a role in MHC isoform gene expression.
Assuntos
DNA Complementar/análise , Exercício Físico/fisiologia , Músculo Esquelético/fisiologia , Cadeias Pesadas de Miosina/metabolismo , Esforço Físico , RNA Mensageiro/análise , Análise de Variância , Biomarcadores/análise , Estudos de Casos e Controles , Estudos de Coortes , Teste de Esforço/métodos , Feminino , Expressão Gênica , Humanos , Masculino , Fibras Musculares Esqueléticas/fisiologia , Cadeias Pesadas de Miosina/análise , Probabilidade , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Regulação para CimaRESUMO
It is now widely recognized that in order to optimize bone health in the later years, bone healthy behaviors should begin at a young age and continue throughout life. Prescribed orally to lower lipid levels in adults of all ages, statins have also been shown to stimulate bone formation in vitro by promoting bone morphogenic protein-2 (BMP-2) activity and to stimulate bone formation in vivo. Weight bearing exercise is well known to stimulate bone formation through a mechanism whereby mechanical loading is 'sensed' by the mechano-sensors leading to a cascade of events involving the activation of osteoblasts. For individuals with high cholesterol levels, both of these interventions are recommended throughout adult life. Since statins and exercise stimulate bone formation via different mechanisms, we hypothesized that exercise in combination with oral simvastatin synergistically increases bone mineral density and strength. Mature adult female, Sprague Dawley rats were divided into 4 groups: control (n=9), statin only (n=8), exercise only (n=11), and statin plus exercise (n=11). Simvastatin was given to the two groups at a dose of 10 mg/kg/day in standard rat chow for the entire 5 week period. All rats ate the same mass of food. The two exercise groups ran on a treadmill with progressively greater speeds and time, ending on week 5 at 30 m/min for 60 min. After 5 weeks, rats were euthanized, and excised femurs were scanned for areal bone mineral density (BMD) and tested by three point bending to obtain the following performance measures: maximum force (strength), stiffness, and work-to-fracture. Only the group treated with statins and exercise showed a positive effect on the biomechanical performance of the femurs. Compared to controls, this group had increased maximum force, stiffness, moment of inertia, and BMD. Linear regression analysis revealed that the increased performance was related to increased BMD. We conclude that the combination of oral statins and appropriate exercise increases bone strength better than either individual treatment and may provide optimal protection against osteoporosis.