Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice.

The aim of this study is to examine the ability of resveratrol to counteract hexavalent chromium [Cr(VI)]-induced genetic damage, as well as the possible pathways associated with this protection. Hsd:ICR male mice are divided into groups of the following five individuals each: (a) control 1, distilled water; (b) control 2, ethanol 30%; (c) resveratrol, 50 mg/kg by gavage; (d) CrO3, 20 mg/kg intraperitoneally; (e) resveratrol + CrO3, resveratrol administered 4 h prior to CrO3. The assessment is performed on peripheral blood. Micronuclei (MN) kinetics are measured from 0 to 72 h, while 8-hydroxydeoxyguanosine (8-OHdG) adduct repair levels, endogenous antioxidant system biomarkers, and apoptosis frequency were quantified after 48 h. Resveratrol reduces the frequency of Cr(VI)-induced MN and shows significant effects on the 8-OHdG adduct levels, suggesting that cell repair could be enhanced by this polyphenol. Concomitant administration of resveratrol and Cr(VI) results in a return of the activities of glutathione peroxidase and catalase to control levels, accompanied by modifications of superoxide dismutase activity and glutathione levels. Thus, antioxidant properties might play an important role in resveratrol-mediated inhibition of Cr(VI)-induced oxidant genotoxicity. The increase in apoptotic cells and the decrease in necrosis further confirmed that resveratrol effectively blocks the actions of Cr(VI).

Antigenotoxic effects of (-)-epigallocatechin-3-gallate (EGCG), quercetin, and rutin on chromium trioxide-induced micronuclei in the polychromatic erythrocytes of mouse peripheral blood.

This study was conducted to investigate the modulating effects of (-)-epigallocatechin-3-gallate (EGCG), quercetin, and rutin on the genotoxic damage induced by Cr(VI) in polychromatic erythrocytes of CD-1 mice. The animals were divided into the following groups: (i) vehicle only; (ii) flavonoids (10 mg/kg EGCG, 100 mg/kg quercetin, 625 mg/kg rutin, or 100-625 mg/kg quercetin-rutin); (iii) Cr(VI) (20 mg/kg of CrO3); and (iv) flavonoids concomitantly with Cr(VI). All of the treatments were administered intraperitoneally (i.p.). The genotoxic damage was evaluated based on the number of micronucleated polychromatic erythrocytes (MN-PCE) obtained from the caudal vein 0, 24, 48, and 72 h after treatment. Groups treated with EGCG and quercetin exhibited no significant statistical changes in induction of MN-PCE. However, CrO3 treatment significantly increased MN-PCE induction 24 and 48 h after injection. Treatment with flavonoids prior to CrO3 exposure decreased MN-PCE induction compared with CrO3 only. The magnitudes of the potency of flavonoids were in the following order: rutin (82%) > quercetin (64%) > quercetin-rutin (59%) and EGCG (44%). The group treated with rutin significantly reduced genotoxic damage in mice treated with Cr(VI) (antioxidant effect). However rutin exerted a marginal genotoxic effect when administered alone (pro-oxidant effect). Our findings suggest protective effects of EGCG, quercetin, and rutin against genotoxic damage induced by Cr(VI).

[The role of resveratrol on heavy metal-induced oxidative stress]. / El papel del resveratrol sobre el estrés oxidante inducido por metales pesados.

INTRODUCTION: Introduction and objetives: oxidative stress is considered one of the main mechanisms of genotoxicity and carcinogenicity of heavy metals. In contrast, resveratrol has antioxidant properties and is one of the most studied polyphenols due to its wide variety of beneficial health effects. However, there are no systematic reviews of the scientific literature in which the effects of resveratrol on oxidative stress induced by heavy metals are analyzed. Methods: in this review, articles were searched using the PubMed and ScienceDirect databases (1996-2018). After applying various filters, eleven in vivo and in vitro researches were considered, in which the effects of resveratrol on oxidative stress induced by arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr) and iron (Fe) were studied. Results: this review presents an analysis of the chemical effects of resveratrol on oxidative stress associated with the exposure of metal compounds. The interaction of resveratrol with the production of reactive oxygen species (ERO's), the endogenous antioxidant system and its effects on DNA damage is discussed. From these studies, a diagram that shows the proposed interactions for resveratrol; heavy metals As, Cd, Cu, Cr and Fe; and oxidative stress is generated. Conclusions: the studies analyzed show that resveratrol is able to modulate the oxidative stress generated by different heavy metal compounds such as As, Cd, Cu, Cr and Fe.

INTRODUCCIÓN: Introducción y objetivos: el estrés oxidante es considerado uno de los principales mecanismos de genotoxicidad y carcinogenicidad de los metales pesados. Por otra parte, el resveratrol posee propiedades antioxidantes y es uno de los polifenoles más estudiados debido a su gran variedad de efectos benéficos para la salud. Sin embargo, no hay revisiones sistemáticas de la literatura científica en las que se analicen los efectos del resveratrol sobre el estrés oxidante inducido por metales pesados. Métodos: en esta revisión, se realizó una búsqueda de artículos mediante las bases de datos PubMed y ScienceDirect (1996-2018). Después de aplicar diversos filtros, se consideraron once investigaciones in vivo e in vitro, en las que se estudiaron los efectos del resveratrol sobre el estrés oxidante inducido por el arsénico (As), cadmio (Cd), cobre (Cu), cromo (Cr) y hierro (Fe). Resultados: en la revisión se presenta un análisis de los efectos químicos del resveratrol sobre el estrés oxidante asociado a la exposición de compuestos metálicos. Se discute la interacción del resveratrol con la producción de especies reactivas de oxígeno (ERO's), el sistema antioxidante endógeno y sus efectos sobre el daño al ADN. A partir de estos estudios se genera un diagrama que muestra las interacciones propuestas para el resveratrol; los metales pesados As, Cd, Cu, Cr y Fe; y el estrés oxidante. Conclusiones: los estudios analizados muestran que el resveratrol es capaz de modular el estrés oxidante generado por diferentes compuestos de metales pesados como los As, Cd, Cu, Cr y Fe.