Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
1.
J Infect Dis ; 229(Supplement_2): S275-S284, 2024 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-38164967

RESUMO

Monkeypox virus (MPXV) is a reemerging virus of global concern. An outbreak of clade I MPXV affected 20 captive chimpanzees in Cameroon in 2016. We describe the epidemiology, virology, phylogenetics, and clinical progression of this outbreak. Clinical signs included exanthema, facial swelling, perilaryngeal swelling, and eschar. Mpox can be lethal in captive chimpanzees, with death likely resulting from respiratory complications. We advise avoiding anesthesia in animals with respiratory signs to reduce the likelihood of death. This outbreak presented a risk to animal care staff. There is a need for increased awareness and a One Health approach to preparation for outbreaks in wildlife rescue centers in primate range states where MPXV occurs. Control measures should include quarantining affected animals, limiting human contacts, surveillance of humans and animals, use of personal protective equipment, and regular decontamination of enclosures.


Assuntos
Monkeypox virus , Pan troglodytes , Animais , Humanos , Camarões , Surtos de Doenças , Animais Selvagens
2.
Arch Virol ; 164(9): 2359-2366, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31240484

RESUMO

Rodent adenoviruses are important models for human disease. In contrast to the over 70 adenovirus types isolated from humans, few rodent adenoviruses are known, despite the vast diversity of rodent species. PCR and Sanger sequencing were used to investigate adenovirus diversity in wild rodents and shrews in Cameroon. Adenovirus DNA was detected in 13.8% of animals (n = 218). All detected sequences differ from known adenovirus types by more than 10% at the amino acid level, thus indicating up to 14 novel adenovirus species. These results highlight the diversity of rodent adenoviruses, their phylogeny, and opportunities for studying alternative adenovirus rodent models.


Assuntos
Infecções por Adenoviridae/veterinária , Adenoviridae/isolamento & purificação , DNA Viral/genética , Variação Genética , Doenças dos Roedores/virologia , Musaranhos/virologia , Adenoviridae/classificação , Adenoviridae/genética , Infecções por Adenoviridae/virologia , Animais , Camarões , Filogenia , Roedores/virologia
3.
Intervirology ; 61(4): 155-165, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30448834

RESUMO

OBJECTIVE: Herpesviruses belong to a diverse order of large DNA viruses that can cause diseases in humans and animals. With the goal of gathering information about the distribution and diversity of herpesviruses in wild rodent and shrew species in central Africa, animals in Cameroon and the Democratic Republic of the Congo were sampled and tested by PCR for the presence of herpesvirus DNA. METHODS: A broad range PCRs targeting either the Polymerase or the terminase gene were used for virus detection. Amplified products from PCR were sequenced and isolates analysed for phylogenetic placement. RESULTS: Overall, samples of 1,004 animals of various rodent and shrew species were tested and 24 were found to be positive for herpesvirus DNA. Six of these samples contained strains of known viruses, while the other positive samples revealed DNA sequences putatively belonging to 11 previously undescribed herpesviruses. The new isolates are beta- and gammaherpesviruses and the shrew isolates appear to form a separate cluster within the Betaherpesvirinae subfamily. CONCLUSION: The diversity of viruses detected is higher than in similar studies in Europe and Asia. The high diversity of rodent and shrew species occurring in central Africa may be the reason for a higher diversity in herpesviruses in this area.


Assuntos
DNA Viral/análise , Variação Genética , Herpesviridae/classificação , Herpesviridae/isolamento & purificação , Roedores/virologia , Musaranhos/virologia , Animais , Ásia , Camarões , DNA Viral/genética , República Democrática do Congo , Herpesviridae/genética , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA
4.
Influenza Other Respir Viruses ; 18(5): e13313, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38757747

RESUMO

BACKGROUND: Influenza and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are both respiratory viruses with similar clinical manifestations and modes of transmission. This study describes influenza data before and during the coronavirus disease pandemic (COVID-19) in Cameroon and SARS-CoV-2 data during the pandemic period. METHODS: The study ran from 2017 to 2022, and data were divided into two periods: before (2017-2019) and during (2020-2022) the COVID-19 pandemic. Nasopharyngeal samples collected from persons with respiratory illness were tested for influenza using the Centers for Disease Control and Prevention (CDC) typing and subtyping assays. During the COVID-19 pandemic, the respiratory specimens were simultaneously tested for SARS-CoV-2 using the DaAn gene protocol or the Abbott real-time SARS-CoV-2 assay. The WHO average curve method was used to compare influenza virus seasonality before and during the pandemic. RESULTS: A total of 6246 samples were tested. Influenza virus detection rates were significantly higher in the pre-pandemic period compared to the pandemic period (30.8% vs. 15.5%; p < 0.001). Meanwhile, the SARS-CoV-2 detection rate was 2.5%. A change in the seasonality of influenza viruses was observed from a bi-annual peak before the pandemic to no clear seasonal pattern during the pandemic. The age groups 2-4 and 5-14 years were significantly associated with higher influenza positivity rates in both pre-pandemic and pandemic periods. For SARS-CoV-2, all age groups above 15 years were the most affected population. CONCLUSION: The COVID-19 pandemic had a significant impact on the seasonal influenza by changing the seasonality of the virus and reducing its detection rates.


Assuntos
COVID-19 , Influenza Humana , SARS-CoV-2 , Humanos , Camarões/epidemiologia , Influenza Humana/epidemiologia , Influenza Humana/virologia , COVID-19/epidemiologia , COVID-19/diagnóstico , COVID-19/virologia , Adolescente , Adulto , Criança , Pré-Escolar , Pessoa de Meia-Idade , Adulto Jovem , Feminino , Masculino , SARS-CoV-2/genética , SARS-CoV-2/isolamento & purificação , Lactente , Idoso , Nasofaringe/virologia , Estações do Ano , Pandemias , Orthomyxoviridae/isolamento & purificação , Orthomyxoviridae/genética , Orthomyxoviridae/classificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA