RESUMO
We summarized available data concerning contamination of the Czech part of the Elbe River by mercury and assessed the potential risks to humans connected with the consumption of contaminated fish. Total mercury concentrations were evaluated in dorsal muscle of chub (Squalius cephalus) and bream (Abramis brama) that were collected at 11 sampling sites in the period from 1991 to 2016. Significant differences among sites were found for both species. The highest contamination was observed near the non-ferrous Kovohute metal works at Celakovice and also downstream from the Spolana chemical plant at Neratovice. A significant decline in contamination with time (concentrations decreasing from 1.65 to 0.22 mg kg-1; p = 0.004) was revealed in chub downstream from the Spolana plant. Calculated hazard indices (HIs) were below 1, therefore, adverse health effects on the general Czech population connected with the consumption of fish from the Elbe River were not expected.
Assuntos
Monitoramento Ambiental/métodos , Mercúrio/análise , Rios/química , Poluentes Químicos da Água/análise , Animais , Cyprinidae/crescimento & desenvolvimento , República Tcheca , Contaminação de Alimentos/análise , Humanos , Músculos/química , Medição de Risco , Análise Espaço-TemporalRESUMO
OBJECTIVES: Effect of long-term oral administration of three different concentrations (0.5, 1.0, and 2.0%) of micronized ß-1.3/1.6-D-glucan derived from oyster mushroom (Pleurotus ostreatus, Hiratake) on biometrical, haematological, biochemical, and immunological indices of half-year-old rainbow trout (Oncorhynchus mykiss) was assessed in the study. DESIGN: Rainbow trout were feed commercial feed pellets containing ß-1.3/1.6-D-glucan in the concentrations of 0.5, 1.0, and 2.0% for 85 days. Biometrical indices consisted in total and standard length, body and liver weight, from which derived somatic parameters such as Fulton´s condition factor and hepatosomatic index were calculated. Haematological parameters were evaluated according to unified methods for haematological examination in fish. Plasma biochemical profile was analysed using biochemical analyser Konelab 20i and Easy Lyte Analyzer. A phagocyte cells metabolic activity (induced chemiluminescence of phagocytes) was determined as an immunological parameter by a microplate luminometric method on Immunotech LM-01T. RESULTS: No clinical signs of behavioral, respiratory, or neurologic distress were observed in rainbow trout. Fish showed normal feeding behavior. As for biometric parameters, no significant changes in total and standard length, body weight, liver weight, as well as in condition factor and hepatosomatic index of experimental and control fish were found. In the course of the study, weight gains in rainbow trout were similar and continuous. Shifts in PCV (p<0.05), haemoglobin (p<0.05), and MCHC (p<0.01) were found within haematological indices. Plasma concentration of glucose, lactate, total protein, cholesterol, calcium, natrium, potassium (all p<0.05), albumins and chlorides (both p<0.01), as well as catalytic activities of ALT and AST (both p<0.05) were changed in the course of the study. A phagocyte cells metabolic activity (luminol-induced chemiluminescence) in rainbow trout was not altered by oyster mushroom ß-1.3/1.6-D-glucan administration. CONCLUSION: After long-term oral administration of three concentrations of micronized ß-1.3/1.6-D-glucan derived from oyster mushroom (Pleurotus ostreatus, Hiratake) shifts in haematological and biochemical profiling were found in half-year-old rainbow trout (O. mykiss) in environmental conditions of a commercial rainbow trout fishery. Biometrical indices were not found significantly altered. No specific effect of ß-glucan on immune system response of rainbow trout was found in the study. The use of ß-glucan in prosperous, clinically healthy aquaculture is still an issue, nevertheless, its use in breedings endangered by stress stimuli, infectious diseases or adverse environmental factors is indisputable.
Assuntos
Ração Animal , Pesqueiros/métodos , Oncorhynchus mykiss/crescimento & desenvolvimento , Pleurotus/química , beta-Glucanas/farmacologia , Adjuvantes Imunológicos/farmacologia , Animais , Proteínas Sanguíneas/metabolismo , Relação Dose-Resposta a Droga , Sistema Imunitário/efeitos dos fármacos , Oncorhynchus mykiss/sangue , Oncorhynchus mykiss/imunologiaRESUMO
We present five families of paediatric patients suffering from choroid plexus carcinoma in which we found germline TP53 mutations. Only one of the families conformed to the criteria of Li-Fraumeni syndrome and only three (including the Li-Fraumeni syndrome family) met the Chompret criteria for germline TP53 mutation testing. In the remaining two families no family history of cancer was identified and/or the parents of the patient were shown not to carry the mutation. Our results give further support to the notion that the occurrence of this rare paediatric tumour, especially in combination with a positive family history of cancer, but possibly also without any family history, may be an indicator of a germline TP53 mutation. The identification of this genetic defect has important consequences for cancer prevention and treatment in affected families.
Assuntos
Neoplasias do Plexo Corióideo/genética , Genes p53/genética , Mutação em Linhagem Germinativa/genética , Adolescente , Adulto , Sequência de Aminoácidos/genética , Criança , Feminino , Humanos , Masculino , Mutação de Sentido Incorreto/genética , LinhagemRESUMO
We describe a girl with neurofibromatosis type 1 (NF1), mild dysmorphic features, growth and mental retardation, autism, and mosaicism of ring chromosome 17 and chromosome 17 monosomy. The extent of genetic material deleted from the ring chromosome was determined using a combination of classical cytogenetics, fluorescence in situ hybridization (FISH) and multiplex ligation-dependent probe amplification (MLPA) to be 0.6-2.5 Mb on 17p, and up to about 10 Mb on 17q. Based on our observations and on a review of the literature we argue that in addition to a universal "ring syndrome" which is based on ring instability and is less specific for the chromosome involved, various ring chromosomes underlie their own characteristic phenotypes. We propose that the symptoms leading to the diagnosis of NF1 in our patient could be attributed to mosaic hemizygosity for the NF1 gene in some of her somatic cells. A similar mechanism or a direct involvement of respective disease genes in the aberration could possibly influence also the development of autism and other symptoms. We raise a question if the loss of one copy of chromosome 17 from a substantial fraction of somatic cells can have specific consequences also for future risks of the patient, for example, due to the mosaic hemizygosity for the BRCA1 and TP53 genes.
Assuntos
Transtorno Autístico/diagnóstico , Transtorno Autístico/genética , Cromossomos Humanos Par 17/genética , Mosaicismo , Neurofibromatose 1/diagnóstico , Neurofibromatose 1/genética , Cromossomos em Anel , Transtorno Autístico/complicações , Criança , Feminino , Humanos , Hibridização in Situ Fluorescente , Neurofibromatose 1/complicaçõesRESUMO
In search of potential prognostic markers, we analyzed a large series of tissues of Barrett's esophagus and samples of adenocarcinomas arising in the terrain of Barrett's esophagus for TP53 gene mutations by direct sequencing of exons 5 to 9 of the TP53 gene. While 9 of 21 adenocarcinomas tested (42.9%) contained a TP53 mutation, none of 24 samples from Barrett's esophagus were mutated. This observation suggests that TP53 gene mutation may be a relatively late event in the progression from nondysplastic Barrett's esophagus to adenocarcinoma of esophagus. Therefore, TP53 gene mutations alone are not likely to represent a widely useful prognostic marker of the risk of progression to malignancy, at least not in Barrett's esophagus without dysplasia.
Assuntos
Esôfago de Barrett/genética , DNA/genética , Genes p53/genética , Mutação , Adenocarcinoma/genética , Adenocarcinoma/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Esôfago de Barrett/patologia , Biópsia , Progressão da Doença , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/patologia , Éxons , Feminino , Marcadores Genéticos , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Lesões Pré-Cancerosas/genética , Lesões Pré-Cancerosas/patologia , Estudos Retrospectivos , Fatores de RiscoRESUMO
BACKGROUND: diGeorge syndrome is a relatively common congenital disorder with developmental defects, including hypoplasia or pathologic migration of the thymus, associated with deletion of contiguous genes on chromosome 22. We prospectively followed a cohort of children with confirmed 22q11.2 deletion. MATERIAL/METHODS: One to six repeated examination were performed in 13 boys and 21 girls, age 4 days to 19 years. Due to the proposed role of the thymus in T lymphocyte selection, we studied T lymphocytes and their function, and also the presence of double positive CD4+CD8+ and gamma/delta T lymphocytes in peripheral blood. RESULTS: A low number of T lymphocytes was detected in the majority of patients before the age of 2 years. Both spontaneous and PHA-induced proliferation were unexpectedly higher than in normal samples from children <2 years old. Both T cell numbers and function normalized thereafter in the majority of patients. Double positive T cells were detected in one boy, together with transient positivity of antinuclear antibodies. Gamma/delta T cells were greater than 5% in 21% of the children. In our 5-year prospective study we have not yet observed serious clinical signs of immunodeficiency or autoimmunity in these patients, except for repeated respiratory infections. CONCLUSIONS: All patients classified as partial diGeorge syndrome presented with delayed but gradual development of immune function against a background of impaired support by the thymus.