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Introduction: Oral cancer refers to malignant tumors, of which 90% are squamous cell carcinomas (OSCCs). These malignancies exhibit rapid progression, poor prognosis, and often mutilating therapeutical approaches. The determination of a prophylactic and/or therapeutic antitumor role of the polyphenolic extract Polypodium leucotomos(PL) would be relevant in developing new tools for prevention and treatment. Methods: We aimed to determine the antitumor effect of PL by treating OSCC cell lines with PL metabolites and evaluating its action during OSCC progression in vivo. Results: PL treatment successfully impaired cell cycling and proliferation, migration, and invasion, enhanced apoptosis, and modulated macrophage polarization associated with the tumoral immune-inflammatory response of tongue cancer cell lines (TSCC). PL treatment significantly decreased the expression of MMP1 (p < 0.01) and MMP2 (p < 0.001), and increased the expression of TIMP1 (p < 0.001) and TIMP2 (p < 0.0001) in these cells. The mesenchymal-epithelial transition phenotype was promoted in cells treated with PL, through upregulation of E-CAD (p < 0.001) and reduction of N-CAD (p < 0.05). PL restrained OSCC progression in vivo by inhibiting tumor volume growth and decreasing the number of severe dysplasia lesions and squamous cell carcinomas. Ki-67 was significantly higher expressed in tongue tissues of animals not treated with PL(p < 0.05), and a notable reduction in Bcl2 (p < 0.05) and Pcna (p < 0.05) cell proliferation-associated genes was found in dysplastic lesions and TSCCs of PL-treated mice. Finally, N-cad(Cdh2), Vim, and Twist were significantly reduced in tongue tissues treated with PL. Conclusion: PL significantly decreased OSCC carcinogenic processes in vitro and inhibited tumor progression in vivo. PL also appears to contribute to the modulation of immune-inflammatory oral tumor-associated responses. Taken together, these results suggest that PL plays an important antitumor role in processes associated with oral carcinogenesis and may be a potential phytotherapeutic target for the prevention and/or adjuvant treatment of TSCCs.
RESUMO
Background: Trained subjects have difficulty in achieving continued results following years of training, and the manipulation of training variables through advanced resistance training (RT) methods is widely recommended to break through plateaus. Objective: The purpose of the present study was to compare the acute effects of traditional RT (TRT) versus two types of sarcoplasma stimulating training (SST) methods on total training volume (TTV), lactate, and muscle thickness (MT). Methods: Twelve trained males (20.75 ± 2.3 years; 1.76 ± 0.14 meters; body mass = 79.41 ± 4.6 kg; RT experience = 4.1 ± 1.8 years) completed three RT protocols in a randomly sequenced order: TRT, SST contraction type (SST-CT), or SST rest interval variable (SST-RIV) with 7 days between trials in arm curl (elbow flexors) and triceps pulley extension (elbow extensors) performed on the same day. Results: The SST groups displayed greater acute biceps and triceps brachii (TB) MT versus the TRT session, with no difference in lactate levels between them. The SST-CT resulted in greater biceps and TB MT versus the SST-RIV session. The TTV was greater for the TRT session versus the SST sessions, except in the case of the elbow flexors (no difference was observed between TRT and SST-CT), and higher for the SST-CT versus the SST-RIV. Conclusion: Trained subjects may benefit from using the SST method as this method may offer a superior MT stimulus and reduced training time, even with a lower TTV.
RESUMO
RESUMO O treinamento de forca (TF) com supervisão pode afetar a carga total levantada (CTL), o número total de repetições (NTR), o tempo líquido sob tensão (TLT) e a percepção subjetiva de esforço (PSE) em indivíduos experientes em TF. O objetivo do presente estudo foi avaliar o efeito da supervisão do personal trainer na sessão de indivíduos experientes em TF. Quinze homens (20,0±2 anos; 176,0±4 cm; 79,3±4,7 kg) experientes com o TF realizaram duas sessões compostas com exercícios para o corpo todo. Na sessão sem supervisão do personal trainer (SPT) os sujeitos auto selecionaram as intensidades utilizadas nos exercícios e foram orientados à "selecionar a sobrecarga que eles tipicamente utilizam para realizar 10 repetições"; na sessão com a supervisão do personal trainer (CPT) os sujeitos auto selecionaram as intensidades utilizadas nos exercícios e foram orientados à "realizar o máximo de esforço possível". Foi observada maior CTL (P<0,001, Δ%=30), NTR (P<0,001, Δ%=29), TLT (P=0,003, Δ%=21) e PSE (P<0,001, Δ%=29) na sessão de TF. O presente estudo concluiu que as sessões com supervisão de um personal trainer afetam positivamente as variáveis do treinamento.
ABSTRACT Supervised resistance training may affect several acute variables such as volume load (VL), the maximum number of repetitions (NRM), time under tension (TUT), and rate of perceived exertion (RPE) in resistance trained-men. The present study aimed to evaluate the influence of the personal trainer's supervision on a resistance training session in resistance trained-men. Fifteen resistance-trained men (20,0±2 years; 176,0±4 cm; 79,3±4,7 kg) performed two training sessions composed of whole-body exercises. In the session without personal trainer's supervision (NPT) the subjects self-selected their loads for each exercise and were oriented to "select a load typically employed to perform 10 repetitions"; in the session with personal trainer's supervision (WPT) the subjects self-selected their loads for each exercise and were oriented to "perform maximum effort". It was observed greater VL (P<0,001, Δ%=30), NRM (P<0,001, Δ%=29), TUT (P=0,003, Δ%=21), and RPE (P<0,001, Δ%=29) in the resistance training session WPT. The present study concluded that supervised sessions positively affect resistance training variables.