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1.
PLoS One ; 17(1): e0262312, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34995319

RESUMO

Yellow fever is endemic in Ghana and outbreaks occur periodically. The prodromal signs due to Yellow Fever Virus (YFV) infection are non-specific, making clinical signs unreliable as the sole criteria for diagnosis. Accurate laboratory confirmation of suspected yellow fever cases is therefore vital in surveillance programs. Reporting of ELISA IgM testing results by laboratories can delay due to late arrival of samples from the collection sites as well as limited availability of ELISA kits. In this study, the diagnostic performance characteristics of a rapid immunochromatographic Standard Q Yellow Fever IgM test kit (SD Biosensor) was evaluated for the rapid diagnosis of Yellow Fever infection in Ghana. A panel of 275 sera, comprising 81 confirmed YFV positives and 194 negatives were re-tested in this study using the Standard Q Yellow Fever IgM test kit. Using the CDC/WHO Yellow Fever IgM capture ELISA as a benchmark, the sensitivity, specificity and accuracy of the Standard Q Yellow Fever test kit were 96.3%, 97.9% and 97.5%, respectively. The false positivity rate was 5.1% and there was no cross-reactivity when the Standard Q Yellow Fever test kit was tested against dengue, malaria and hepatitis B and C positive samples. In addition, inter-reader variability and invalid rate were both zero. The results indicate that the diagnostic performance of the Standard Q Yellow Fever IgM test kit on serum or plasma is comparable to the serum IgM detection by ELISA and can be used as a point of care rapid diagnostic test kit for YFV infection in endemic areas.


Assuntos
Técnicas Biossensoriais/instrumentação , Cromatografia de Afinidade/instrumentação , Imunoglobulina M/imunologia , Kit de Reagentes para Diagnóstico , Febre Amarela/diagnóstico , Vírus da Febre Amarela/imunologia , Técnicas Biossensoriais/economia , Cromatografia de Afinidade/economia , Desenho de Equipamento , Humanos , Imunoglobulina M/sangue , Limite de Detecção , Kit de Reagentes para Diagnóstico/economia , Fatores de Tempo , Febre Amarela/sangue , Febre Amarela/imunologia , Vírus da Febre Amarela/isolamento & purificação
2.
Biomed Res Int ; 2021: 1031965, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660777

RESUMO

In this study, the seroprevalence of the intestinal worms Taenia solium and Trichinella spiralis in humans and pigs was assessed. A cross-sectional serological study design was performed. Blood samples were collected from 322 humans and 245 pigs used in the study. These were tested for markers of antibodies for Taenia solium and Trichinella spp. Demographic data such as sex, age, education, pig farming practices, and water source used were also obtained. An overall seroprevalence of 3.1% was recorded for Taenia solium in humans. There was also a statistical association between pig management system employed by pig farmers and seropositivity to Taenia solium (p = 0.005). Factors such as mode of waste disposal (p = 0.003) and water source used statistically correlated with Taenia solium seroprevalence among humans. For the pig samples, a Taenia solium seroprevalence of 24.9% was recorded. All the pig samples which tested positive for Taenia solium were reared on the free-ranged system. This study also recorded a seroprevalence of 0.31% for Trichinella spp. for humans and a seroprevalence of 4.5% for Trichinella spp. for pigs. Again, all the samples that showed serological evidence of Trichinella spp. among pigs came from those pigs which were raised on the free-ranged system. Proper pig management practice is a very important tool for controlling these intestinal parasites in both humans and animals. This study recommends public health education among the general public and good pig farming practices.


Assuntos
Anticorpos Anti-Helmínticos/sangue , Cisticercose/parasitologia , Saúde Pública/métodos , Taenia solium/isolamento & purificação , Trichinella spiralis/isolamento & purificação , Triquinelose/parasitologia , Gerenciamento de Resíduos/métodos , Adulto , Animais , Estudos Transversais , Cisticercose/sangue , Cisticercose/epidemiologia , Cisticercose/patologia , Feminino , Gana/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Suínos , Triquinelose/sangue , Triquinelose/epidemiologia , Triquinelose/patologia , Adulto Jovem
3.
Pan Afr Med J ; 38: 402, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34381546

RESUMO

INTRODUCTION: accurate and timely laboratory diagnosis of yellow fever (YF) is critical to the Eliminate Yellow Fever Epidemics (EYE) strategy. Gavi, the Vaccine Alliance recognized the need to support and build capacity in the national and regional laboratories in the Global YF Laboratory Network (GYFLN) as part of this strategy. METHODS: to better understand current capacity, gaps and needs of the GYFLN laboratories in Africa, assessments were carried out in national and regional reference laboratories in the 25 African countries at high risk for YF outbreaks that were eligible for new financial support from Gavi. RESULTS: the assessments found that the GYFLN in Africa has high capacity but 21% of specimens were not tested due to lack of testing kits or reagents and approximately 50% of presumptive YF cases were not confirmed at the regional reference laboratory due to problems with shipping. CONCLUSION: the laboratory assessments helped to document the baseline capacities of these laboratories prior to Gavi funding to support strengthening YF laboratories.


Assuntos
Surtos de Doenças , Laboratórios/estatística & dados numéricos , Febre Amarela/diagnóstico , África/epidemiologia , Fortalecimento Institucional , Epidemias , Humanos , Febre Amarela/epidemiologia
4.
Int J Mycobacteriol ; 4(3): 184-90, 2015 09.
Artigo em Inglês | MEDLINE | ID: mdl-27649864

RESUMO

BACKGROUND: Buruli ulcer (BU) disease, a skin condition caused by Mycobacterium ulcerans (M. ulcerans) is endemic in remote rural areas. Disease diagnosis on clinical basis alone can be misleading, requiring definitive diagnosis based on laboratory tests. Resource constraints in BU endemic areas make microscopy for the detection of acid fast bacilli (AFB) an important and useful method. It is rapid, user-friendly, convenient and cheap. Despite its usefulness, its performance is relatively low. This study investigated modifications of the current method aimed at improving its performance. Forty (IS) 2404 polymerase chain reactions (PCR) positive BU samples were processed by eight physical (centrifugation and overnight sedimentation) and chemical (phenol ammonium sulphate and sodium hypochlorite) modifications of the current direct method. Assessments were based on standard AFB evaluation coupled with in house criteria; positivity (P), clarity and contrast (C) release of bacilli from specimen (R). Overall AFB positivity rate was 64% (409/640). Each protocol had 80 smears. The percentage positivity (P) for the conventional method was 58% (46/80) smears. The highest positivity rate of 57/80 (%) was by protocol 7 (5% phenol in 4% ammonium sulphate (PhAS) and concentrated by overnight gravitational sedimentation). The least positivity rate at 35% (28/80) was by protocol 1 (smears from direct application of swab tips). The differences in performance between the two chemical tested; 5% phenol in 4% ammonium sulphate (PhAS) and 3.5% NaHOCl was significant (p<0.05). The differences between the two physical methods were however not significant (p>0.05). This study concluded that BU samples treated with a solution of 5% phenol in 4% ammonium sulphate and concentrated by either centrifugation or overnight sedimentation is useful for maximizing AFB detection by bright field microscopy. This can be useful in rural health facilities with resource constraints.


Assuntos
Úlcera de Buruli/diagnóstico , Úlcera de Buruli/microbiologia , Microscopia/estatística & dados numéricos , Serviços de Saúde Rural , Técnicas Bacteriológicas/economia , Técnicas Bacteriológicas/instrumentação , Técnicas Bacteriológicas/estatística & dados numéricos , Úlcera de Buruli/epidemiologia , Análise Custo-Benefício , DNA Bacteriano/isolamento & purificação , Doenças Endêmicas , Gana/epidemiologia , Humanos , Microscopia/economia , Microscopia/instrumentação , Mycobacterium ulcerans/isolamento & purificação , Reação em Cadeia da Polimerase , Manejo de Espécimes
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