Turmeric rhizomes reduced in vitro methane production and improved gas production and nutrient degradability.

The present study aimed to evaluate the effect of dry turmeric rhizomes on in vitro biogas production and diet fermentability. Turmeric rhizomes were included at gradually increased levels: 0, 0.5, 1, 1.5 and 2% of a diet containing per kg dr matter (DM): 500 g concentrate feed mixture, 400 g berseem hay and 100 g rice straw, and incubated for 48 h. Gas chromatography-mass spectrometry analysis showed that ar-turmerone, α-turmerone and ß-turmerone were the major bioactive compounds in the rhizomes. Turmeric rhizomes increased (p < 0.01) asymptotic gas production (GP) and rate and lag of CH4 production and decreased (p < 0.01) rate of GP, lag of GP, asymptotic CH4 production and proportion of CH4 production. Turmeric rhizome administration linearly increased (p < 0.01) DM and fiber degradability and concentrations of total short-chain fatty acids, acetic and propionic acids and ammonia-N and quadratically (p < 0.05) decreased fermentation pH. It is concluded that including up to 2% turmeric rhizomes improved in vitro ruminal fermentation and decreased CH4 production.

Partial replacement of corn with chocolate byproducts or protected fat in diets of early lactating Holstein cows: feed utilization and lactational performance.

This study determined the effects of the partial replacement of corn with chocolate byproducts or protected fat on the performance of cows. Forty multiparous Holstein cows were assigned to four treatments in a completely randomized design for 90 d. The control diet was a complete diet containing 41.2% concentrate feed mixture, 41.2% corn silage, and 17.6% berseem hay. In the other experimental diets, each kg of crushed corn grain in the control diet (DM basis) was replaced with 600 g chocolate byproducts (CH), 400 g protected fat (PF), or 500 g of a mixture of chocolate byproducts and protected fat (1:1 DM basis) (CHPF). Both amounts of PF and CH had the same energy concentration as 1 kg of corn. The experimental treatments did not affect total intake; however, they increased (p < 0.001) daily production of milk, milk fat content, and feed efficiency. Moreover, treatments increased (p < 0.05) blood total protein, albumin, glucose, cholesterol, and triglyceride. The CH, PF, and CHPF treatments increased (p < 0.01) nutrient digestibility. It is concluded that replacing 1 kg of corn in the diet of lactating Holstein cows with PF at 400 g, followed by CHPF at 500 g, and CH at 600 g enhanced milk production and feed efficiency.

The effect of Saccharomyces cerevisiae live cells and Aspergillus oryzae fermentation extract on the lactational performance of dairy cows.

Eighty multiparous lactating Holstein cows (635 ± 33 kg) were used to study the effect of feeding Saccharomyces cerevisiae and/or Aspergillus oryzae on lactational performance for 14 weeks. Cows were assigned in a completely randomized experimental design, with repeated measures into four treatments, and were fed a basal diet of concentrates and forage at a ratio of 592:408, respectively. The treatments were: (1) the basal diet with no additive (Control treatment); (2) the basal diet supplemented with 3.5 g of live S. cerevisiae/cow daily (SC treatment); (3) the basal diet supplemented with 3.5 g A. oryzae fermentation extract/cow daily (AO treatment); and (4) the basal diet supplemented with 3.5 g of live S. cerevisiae + 3.5 g A. oryzae fermentation extract/cow daily (AOSC treatment). The AO and AOSC treatments increased (p < .05) feed intake and daily milk production, with a low milk fat content for the AO treatment. Feeding SC treatment decreased (p = .002) serum glucose concentration, while the AOSC treatment increased serum glutamate-oxaloacetate transaminase concentration. It is concluded that S. cerevisiae supplementation did not enhance milk production; however, A. oryzae fermentation extract improved feed intake and milk production.

Feed utilization and lactational performance of Friesian cows fed beet tops silage treated with lactic acid bacteria as a replacement for corn silage.

Thirty multiparous lactating Friesian cows were used to study the effect of partial or complete replacement of corn silage with lactic acid bacteria (LAB), molasses and calcium carbonate ensiled sugar beet tops for 3 months in a complete randomized experimental design with repeated measures. A week after parturition, cows were grouped into three treatments of 10 cows each and fed a control treatment containing corn silage at 300 g/kg DM. In the other treated diets, 50% or 100% of the control corn silage was substituted with beet tops silage treated with LAB included at 150 g or 300 g per kg diet. Ensiling of beet tops with LAB decreased its contents of oxalic acid and neutral detergent and acid detergent fibers but increased its contents of non-structural carbohydrate and calcium. Without affecting daily milk production and feed efficiency, beet tops silage treated with LAB diets decreased (p < 0.05) feed intake, total ruminal volatile fatty acids, acetate and propionate concentrations, energy-corrected milk yield and concentrations of milk total solids, fat, protein and energy. In conclusion, beet tops silage treated with LAB can replace corn silage in diets of lactating cows. An inclusion rate of 15% of beet tops silage treated with LAB (replacing 50% of corn silage) in the diet was the most suitable level for lactating cows under the current experimental conditions.

Lupin Seed Supplementation as a Functional Feed Additive: In Vitro Ruminal Gas, Methane and Carbon Dioxide Production, Fermentation Kinetics, and Nutrient Degradability.

The inevitable enteric gas emission from ruminants is considered a modern-day problem from an environmental perspective. Addressing this problem requires nutritional approaches such as the use of phytogenic additives in ruminant diets. In this regard, lupin seed (LS) can be a useful additive due to its phytochemical constituents. Therefore, this study investigated the effects of lupin (Lupinus angustifolius) seed supplementation as a functional and sustainable feed additive in sheep diet (50:50 concentrate-to-forage ratio) on in vitro gas production (GP; mL/g DM), methane (CH4; mL/g DM) and carbon dioxide (CO2; mL/g DM) emissions, fermentation parameters, and nutrient degradability (g/kg DM incubated). Gas production and CH4 were measured per gram of incubated dry matter (DM), degradable DM (dDM), degradable neutral detergent fiber (dNDF), and degradable acid detergent fiber (dADF). Lupin seeds were included at 0 (control), 0.5, 1.0, 1.5, and 2% of the diet. The seeds contained 3.27% essential oils (DM basis), with eucalyptol as the main phytochemical. The highest GP per gram of DM and dDM was observed (p < 0.01) with 2.0% LS supplementation level. While 1.0% LS had the highest GP per gram of dNDF, 0.5% lupin diet had the highest GP per gram of dADF. Asymptotic GP and CH4 emissions linearly and quadratically increased (p < 0.01) with increasing LS level, while lag time decreased. Despite increased CH4 production, the proportion of CH4 in total biogas was lower (p = 0.008) for LS treatments than the control, with the 0.5% LS showing the lowest CH4 proportion. Production of CO2 increased with lupin seed treatments, with 0.5% LS producing the highest proportion (p = 0.027). Degradability of DM, NDF, and ADF was greater (p < 0.01) for the high LS supplementation level, while 0.5% supplementation level decreased ADF degradability. Total short-chain fatty acids, acetic acid, and propionic acid increased (p < 0.05) with LS supplementation level, leading to a reduced acetate:propionate ratio. Rumen pH decreased (p = 0.036) with LS supplementation, while ammonia-N decreased (p = 0.045) and estimated metabolizable energy increased (p < 0.001) linearly. Calculated microbial protein synthesis (p = 0.005) and gas yield (p = 0.047) increased with LS supplementation level. LS supplementation at 2.0% of diet (DM basis) increased GP and CH4 emission (mL/g DM) and enhanced nutrient degradability, suggesting its potential use as a functional feed additive for ruminants when supplemented at a 2.0% level into diet.

Multivariate characterisation of morpho-biometric traits of indigenous helmeted Guinea fowl (Numida meleagris) in Nigeria.

This study was conducted to characterise phenotypically helmeted Guinea fowls in three agro-ecologies in Nigeria using multivariate approach. Eighteen biometric characters, four morphological indices and eleven qualitative physical traits were investigated in a total of 569 adult birds (158 males and 411 females). Descriptive statistics, non-parametric Kruskal-Wallis H test followed by the Mann-Whitney U and Dunn-Bonferroni tests for post hoc, Multiple Correspondence Analysis (MCA), Univariate Analysis, Canonical Discriminant Analysis, Categorical Principal Component Analysis and Decision Trees were employed to discern the effects of agro-ecological zone and sex on the morphostructural parameters. Agro-ecology had significant effect (P<0.05; P<0.01) on all the colour traits. In general, the most frequently observed colour phenotype of Guinea fowl had pearl plumage colour (54.0%), pale red skin colour (94.2%), black shank colour (68.7%), brown eye colour (49.7%), white earlobe colour (54.8%) and brown helmet colour (72.6%). The frequencies of helmet shape and wattle size were significantly influenced (P<0.01) by agro-ecology and sex. Overall, birds from the Southern Guinea Savanna zone had significantly higher values (P<0.05) for most biometric traits compared to their Sudano-Sahelian and Tropical Rainforest counterparts. They were also more compact (120.00 vs. 110.00 vs. 107.69) but had lesser condition index (7.66 vs. 9.45 vs. 9.30) and lower long-leggedness (19.71 vs. 19.23 vs. 9.51) than their counterparts from the two other zones. Sexual dimorphism (P<0.05) was in favour of male birds especially those in Southern Guinea Savanna and Sudano-Sahelian zones. However, the MCA and discriminant analysis revealed considerable intermingling of the qualitative physical traits, biometric traits and body indices especially between the Sudano-Sahelian and Tropical Rainforest birds. In spite of the high level of genetic admixture, the Guinea fowl populations could to a relative extent be distinguished using wing length, body length and eye colour. Generally, the birds from the three zones appeared to be more homogeneous than heterogeneous in nature. However, further complementary work on genomics will guide future selection and breeding programs geared towards improving the productivity, survival and environmental adaptation of indigenous helmeted Guinea fowls in the tropics.

Performance and hemtochemical parameters of buck-kids fed concentrate partially replaced with tropical Piliostigma thonningii foliage.

Fifteen 5-month-old Red Sokoto buck-kids, (6.6 ± 0.71 kg body weight (BW)) randomly distributed into three groups of five animals per group, were used to study the effects of supplementary concentrate partially replaced with Piliostigma thonningii (PT) foliage on the growth performance, economic benefit and blood profile in a completely randomized design using analysis of variance. The goats in group 1 received 100% supplementary concentrates (PT0), groups 2 and 3 received 25% (PT25) and 50% (PT50), respectively, of concentrate replaced with an equal amount (dry matter basis) of Piliostigma foliage. The goats were fed a basal diet of threshed sorghum top (TST). Intake of concentrate, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, total feeding cost and cost/kg BW were greater (P < 0.05) for PT0 than for PT25 and PT50. Consumption of P. thonningii foliage was greater (P < 0.05) for PT50 relative to PT25. Tannin consumption of the treatment diets were greater (P < 0.05) than that of the control concentrate diet. Serum urea N reduced (P < 0.05) with increasing level of concentrate replacement, while serum glucose was higher (P < 0.05) in PT0 than in PT50. However, means of all blood measurements were within normal ranges for goats. Net benefit showed this rank order: PT0 < PT50 < PT25 (all P < 0.05). Both differential and relative benefits were higher (P < 0.05) for PT25 than for PT50. P. thonningii foliage can replace 50% of supplemental concentrate without impairing feed intake, growth performance and health of buck-kids.

Sunflower Oil and Nannochloropsis oculata Microalgae as Sources of Unsaturated Fatty Acids for Mitigation of Methane Production and Enhancing Diets' Nutritive Value.

The objective of this assay was to investigate the effect of adding sunflower oil, Nannochloropsis oculata microalgae and their mixture at 0, 1, 2, 3, 4, and 5% to three total mixed rations (TMRs) with different concentrate:forage ratios (40C:60F, 50C:50F, and 60C:40F) on in vitro gas production (GP), methane (CH4) production, and nutrient degradability. Asymptotic GP, GP rate, CH4 concentration/g acid detergent fiber (ADF), dry matter (DM) degradability (DMD), short chain fatty acids (SCFAs), and ruminal bacteria population increased, but neutral detergent fiber (NDF) degradability (NDFD), ADF degradability (ADFD), and protozoa count decreased with increasing concentrate level in the TMR. Methane production/g DM and NDF was higher for 50C:50F TMR. Sunflower oil reduced asymptotic GP, lag time, CH4 production/g ADF, ammonia-N (NH3-N), and SCFA. Compared to the control treatments, additives decreased GP rate, while sunflower oil/N. oculata mixture increased DMD and NDFD. All additives at 5% increased GP rate and lag time and decreased CH4 production/g DM, ADF, and NDF, ruminal NH3-N, and protozoa count. All additives at 2% increased DMD, NDFD and ADFD, SCFA, and bacteria population. Supplementation of TMR, containing different concentrate:forage ratios, with sunflower oil, N. oculata, and sunflower oil/N. oculata mixture at different doses modified in vitro GP, CH4 production, and nutrient degradability.