Approximate Bayesian computation and ecological niche models elucidate the demographic history and current fragmented population distribution of a Korean endemic shrub.

Climatic fluctuations and geological events since the LGM are believed to have significantly impacted the population size, distribution, and mobility of many species that we observe today. In this paper, we determined the processes driving the phylogeographic structure of the Korean endemic white forsythia by combining the use of genome-wide SNPs and predicting paleoclimatic habitats during the LGM (21 kya), Early Holocene (10 kya), Mid-Holocene (6 kya), and Late Holocene (3 kya). Using a maximum of 1897 SNPs retrieved from 124 samples across nine wild populations, five environmental predictors, and the species' natural occurrence records, we aimed to infer the species' demographic history and reconstruct its possible paleodistributions with the use of approximate Bayesian computation and ecological niche models, respectively. Under this integrated framework, we found strong evidence for patterns of range shift and expansion, and population divergence events from the onset of the Holocene, resulting in the formation of its five distinct genetic units. The most highly supported model inferred that after the split of an ancestral population into the southern group and a larger central metapopulation lineage, the latter gave rise to the eastern and northern clusters, before finally dividing into two sub-central groups. While the use of molecular data allowed us to identify and refine the (phylo)genetic relationships of the species' lineages and populations, the use of ecological data helped us infer a past LGM refugium and the directions of post-glacial range dynamics. The time frames of these demographic events were shown to be congruent with climatic and geological events that affected the central Korean Peninsula during these periods. These findings gave us a better understanding of the consequences of past spatiotemporal factors that may have resulted in the current fragmented population distribution of this endangered plant.

Medicinal plants for gastrointestinal diseases among the Kuki-Chin ethnolinguistic groups across Bangladesh, India, and Myanmar: A comparative and network analysis study.

ETHNOPHARMACOLOGICAL RELEVANCE: The Kuki-Chin is a composite ethnolinguistic group made up of many distinct groups and subgroups that are linked by a common history, culture, and population distribution in the Kuki-Chin region, shared geopolitically by Bangladesh, India, and Myanmar. This region is shown to be in a hotspot of digestive disease activity based on official reports and in concordance with data from related ethnopharmacological field surveys. Our work provides a comparative analysis of medicinal plant knowledge for the treatment of gastrointestinal diseases (GAS-Ds) among the Kuki-Chin ethnic groups found across the three countries. AIM OF THE STUDY: The objectives were (1) to identify the medicinal plants and herbal therapies for the treatment of GAS-Ds reported in related Kuki-Chin ethnobotanical publications, (2) to find the similarities and differences in medicinal plant knowledge among the country-affiliated groups by comparing plant and disease information, and (3) to determine significant plant taxa and their pattern(s) of use as digestive disease herbal remedies by analyzing disease-plant networks. MATERIALS AND METHODS: Secondary data were gathered from 34 related studies through internet search of ethnobotanical information from field studies published in journals, reports, books, and dissertations/theses. These data represented 22 Kuki-Chin ethnolinguistic groups inhabiting 23 districts across six states comprising the Kuki-Chin region: the Chittagong Division (Bangladesh); Mizoram, Assam, Manipur, and Tripura States (India); and Chin State (Myanmar). Scientific names of plants and digestive diseases collated from secondary sources were validated and standardized following the Plant List and the International Classification of Primary Care Second Edition, respectively. RESULTS AND DISCUSSION: A total of 482 species in 330 genera and 118 families of vascular plants were recorded for the treatment of 29 standardized GAS-Ds. On the basis of the number of use-reports, regional native species Phyllanthus emblica, Centella asiatica, and Aegle marmelos, as well as the non-natives Carica papaya, Portulaca oleracea, and Psidium guajava were found to be the most preferred. Comparative analysis of medicinal species showed that groups in India revealed the highest number of medicinal plant taxa, followed by those in Bangladesh and Myanmar. The most prevalent GAS-Ds were shown to be abdominal pain epigastric, gastroenteritis presumed infections, and diarrhoea, as supported by the number of use-reports and medicinal taxa used for their treatment, and their informant consensus factor values. Analyses of disease-plant networks graphically showed both GAS-Ds distribution patterns in the region and potential medicinal plant (group) candidates for further studies. CONCLUSION: Overall, this study revealed an astonishingly rich diversity of medicinal plants used to treat digestive diseases among the Kuki-Chin. A comparative analysis showed variations and remarkable distinctions in medicinal plant knowledge among country-affiliated groups, as likely influenced by external sociocultural factors and geopolitical barriers, as well as the availability of ethnobotanical data. The use of network analysis reflected not only shared common affinities and patterns of plant use based on the taxonomic composition and species selection, but also disease prevalence and distribution patterns. The information found here can hopefully spur interest among future researchers and policy makers to better improve both the (ethno) medicinal research capacity and healthcare system in the region.

Ethnomedicinal plants and traditional knowledge among three Chin indigenous groups in Natma Taung National Park (Myanmar).

ETHNOPHARMACOLOGICAL RELEVANCE: This research describes the ethnomedicinal plants as used by three Chin indigenous groups inhabiting areas at Natma Taung National Park (NTNP) in bio-culturally diverse Myanmar. The aim of this study was (1) to identify wild medicinal species and evaluate their local importance in local peoples' healthcare as well as in protected area conservation; and (2) to compare traditional medicinal plant knowledge among the informants. MATERIALS AND METHODS: A total of 206 Müün, Ng'gah and Daai informants from 20 villages were interviewed using semi-structured questionnaires. Species use-reports were computed to determine plant local importance and the informant consensus factor. Descriptive and the inferential statistics Mann-Whitney U and Kruskal-Wallis tests were employed to evaluate and compare the informants' traditional medicinal knowledge. RESULTS: A total of 75 wild ethnomedicinal taxa in 40 plant families across 16 ICPC-based disease categories were recorded. Species which recorded the highest number of use-reports appeared to play an important role not only in informants' primary healthcare and in park conservation, but also in local livelihood. CONCLUSION: This study presents the diversity of ethnomedicinal plants and their local importance in Chin indigenous peoples' healthcare. This paper also recognizes the value of these plants and the local traditional knowledge for the conservation and management of NTNP.

Herbal Therapies and Social-Health Policies: Indigenous Ati Negrito Women's Dilemma and Reproductive Healthcare Transitions in the Philippines.

The high maternal mortality in the Philippines in the past decades prompted intervention strategies to curb unwanted deaths of mothers and improve health and social conditions of women. Such introductions however have begun to challenge traditional reproductive health practices creating confusion among practitioners and incipient transitions in healthcare. Our aim in this study was to document the herbal therapies practiced by indigenous Ati Negrito women and discuss the implications of social and conventional healthcare intervention programs on reproductive healthcare traditions by conducting semistructured interviews. Fidelity Level index was used to determine culturally important plants (i.e., the most preferred). Review of related studies on most preferred plants and therapies was further carried out to provide information regarding their safety/efficacy (or otherwise). Determination of informants' traditional medicinal knowledge was done using Mann-Whitney U and Kruskal-Wallis tests. A total of 49 medicinal plants used in treating female reproductive health-related syndromes across four categories were recorded. Significant differences in traditional medicinal knowledge were recorded when informants were grouped according to age, education, and number of children. Issues discussed in this research could hopefully raise awareness on changes in healthcare practices in indigenous cultures and on medical safety especially when traditional and conventional medications interact.

Quantitative ethnobotanical study of the medicinal plants used by the Ati Negrito indigenous group in Guimaras island, Philippines.

ETHNOPHARMACOLOGICAL RELEVANCE: This study is (1) a documentation of medicinal plant use in traditional therapies, and (2) an evaluation of the medicinal plant knowledge and practices of the Ati Negrito indigenous people in Guimaras Island, Philippines. MATERIALS AND METHODS: A semi-structured interview was conducted to 65 informants in order to determine the medicinal plants and their uses in traditional therapies. The plants were collected, identified and deposited as voucher specimens. Plant importance was determined using quantitative ethnobotanical indices such as Use Value (UV), Fidelity Level (FL) and Informant Consensus Factor (ICF). Descriptive and the inferential statistics Mann-Whitney U and Kruskal-Wallis tests were used to measure and compare the informants׳ medicinal plant use knowledge and practices. RESULTS: This study was able to identify 142 medicinal plant taxa in 55 families used in 16 categories of diseases. Plants with the highest recorded UVs were Psidium guajava (2.52), Blumea balsamifera (2.15) and Cocos nucifera (2.06). A total of 24 species were found to have 100% FL values with Senna alata, Hibiscus rosa-sinensis and Breynia vitis-idaea recording the highest number of use-mentions (48) for treating white spot, boils, and child sleeplessness, malaise and fatigue, respectively. The highest ICF value (1.00) was cited for Category 6 (Diseases of the ear) and Category 16 (Factors that influence health status and services). Finally, significant differences in medicinal plant use knowledge were recorded when informants were grouped according to location, educational level, gender and age. CONCLUSIONS: This documentation of medicinal plants and their uses shows the rich tradition in ethno-medicinal knowledge of the Ati Negrito indigenous people although results might also imply that knowledge is eroding. Nevertheless, this study could open an avenue for pharmacological research works, or serve as reference for future quantitative ethnobotanical investigations.