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Food fortification is an effective strategy to address vitamin A (VitA) deficiency, which is the leading cause of childhood blindness and drastically increases mortality from severe infections. However, VitA food fortification remains challenging due to significant degradation during storage and cooking. We utilized an FDA-approved, thermostable, and pH-responsive basic methacrylate copolymer (BMC) to encapsulate and stabilize VitA in microparticles (MPs). Encapsulation of VitA in VitA-BMC MPs greatly improved stability during simulated cooking conditions and long-term storage. VitA absorption was nine times greater from cooked MPs than from cooked free VitA in rats. In a randomized controlled cross-over study in healthy premenopausal women, VitA was readily released from MPs after consumption and had a similar absorption profile to free VitA. This VitA encapsulation technology will enable global food fortification strategies toward eliminating VitA deficiency.
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Deficiência de Vitamina A , Vitamina A , Feminino , Ratos , Animais , Alimentos Fortificados , Estudos Cross-Over , Culinária , MicronutrientesRESUMO
BACKGROUND: Facebook is a frequently used social media platform and is often used for human health information, yet little research has been conducted on how pet owners use Facebook pet groups to obtain and share pet health information. METHOD: This study was designed to assess how pet owners use dog and cat Facebook groups to provide and receive pet health advice and their perception of these groups' trustworthiness. Two comparable questionnaires (dog and cat owners) were developed and distributed through an online survey platform. RESULTS: Results suggest that Facebook groups are a common source of pet health information, with 56.2% of dog owners and 51.8% of cat owners reporting receiving health information through Facebook groups. Similar numbers report giving health information through Facebook groups: 55.0% of dog owners and 57.9% of cat owners. Dog health information most commonly exchanged related to dermatology, gastroenterology and orthopaedics and the most common cat health information focused on gastroenterology, renal and urinary-related issues. While the majority of Facebook users report feeling that Facebook groups are not a trustworthy source of pet health information, a substantial minority of users do appear to be influenced by these groups. CONCLUSION: Approximately 50% of cat and dog owning respondents either give or receive pet health advice through Facebook groups. These results suggest that many owners deem Facebook groups as useful, but not entirely trustworthy, sources of information.
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Doenças do Gato , Doenças do Cão , Mídias Sociais , Animais , Gatos , Cães , Humanos , Propriedade , Animais de Estimação , Inquéritos e QuestionáriosRESUMO
The NanoRelease Food Additive project developed a catalog to identify potential engineered nanomaterials (ENMs) used as ingredients, using various food-related databases. To avoid ongoing debate on defining the term nanomaterial, NanoRelease did not use any specific definition other than the ingredient is not naturally part of the food chain, and its dimensions are measured in the nanoscale. Potential nanomaterials were categorized based on physical similarity; analysis indicated that the range of ENMs declared as being in the food chain was limited. Much of the catalog's information was obtained from product labeling, likely resulting in both underreporting (inconsistent or absent requirements for labeling) and/or overreporting (inability to validate entries, or the term nano was used, although no ENM material was present). Three categories of ingredients were identified: emulsions, dispersions, and their water-soluble powdered preparations (including lipid-based structures); solid encapsulates (solid structures containing an active material); and metallic or other inorganic particles. Although much is known regarding the physical/chemical properties for these ingredient categories, it is critical to understand whether these properties undergo changes following their interaction with food matrices during preparation and storage. It is also important to determine whether free ENMs are likely to be present within the gastrointestinal tract and whether uptake of ENMs may occur in their nanoform physical state. A practical decision-making scheme was developed to help manage testing requirements.
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Dog "aggression" in the veterinary practice is commonplace. Therefore, student knowledge and education about dog behaviour and the ability to interpret "aggressive" behaviour is important from a human injury prevention and dog welfare perspective. The study aimed to compare first-year veterinary students' perceived safest proximity to both an "aggressive" and non-reactive simulated dog, both before and after a teaching intervention about canine behaviour and a handling practical. It also examined student confidence and their ability to identify "aggressive" behaviours. Forty first year veterinary students took part in two surveys. Each survey included two videos: one of a simulated dog displaying "aggressive" behaviour, based on the 'Canine Ladder of Aggression'; and another displaying non-reactive (passive behaviours without reaction to the participants) behaviours. Each video depicted the slow and consistent approach towards the virtual dog within a virtual indoor environment, and participants were asked to press stop if or when they would stop approaching the dog. In the "aggressive" scenario, there was a reduction in the approach-stop time from survey 1 (median = 17.8 s) to survey 2 (median = 15.2 s) in the intervention group (p = 0.018) but not in the control group (p = 0.147). Regarding confidence, there was a significant increase in the self-reported confidence rating relating to a participant's ability to interpret canine behaviour in both the control (p = 0.011) and intervention (p = 0.003). In conclusion, these results indicate that students using approach-stop videos stayed further away from an "aggressive" virtual dog model if they had undertaken a canine behaviour educational intervention. This novel approach has the potential for further use in teaching and assessment of student knowledge and behaviour which may otherwise be difficult to demonstrate.
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Agressão , Estudantes , Humanos , Cães , Animais , EscolaridadeRESUMO
Wastewater-based monitoring has been widely implemented worldwide for the tracking of SARS-CoV-2 outbreaks and other viral diseases. In many surveillance programmes, unprocessed and processed wastewater samples are often frozen and stored for long periods of time in case the identification and tracing of an emerging health threat becomes necessary. However, extensive sample bioarchives may be difficult to maintain due to limitations in ultra-freezer capacity and associated cost. Furthermore, the stability of viruses in such samples has not been systematically investigated and hence the usefulness of bioarchives is unknown. In this study, we assessed the stability of SARS-CoV-2, influenza viruses, noroviruses and the faecal indicator virus, crAssphage, in raw wastewater and purified nucleic aacid extracts stored at -80 °C for 6-24 months. We found that the isolated viral RNA and DNA showed little signs of degradation in storage over 8-24 months, whereas extensive decay viral and loss of qPCR signal was observed during the storage of raw unprocessed wastewater. The most stable viruses were noroviruses and crAssphage, followed by SARS-CoV-2 and influenza A virus. Based on our findings, we conclude that bioarchives comprised of nucleic acid extracts derived from concentrated wastewater samples may be archived long-term, for at least two years, whereas raw wastewater samples may be discarded after one year.
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Eco-friendly new mosquito control innovations are critical for the ongoing success of global mosquito control programs. In this study, Sh.463_56.10R, a robust RNA interference (RNAi) yeast insecticide strain that is suitable for scaled fermentation, was evaluated under semi-field conditions. Inactivated and dried Sh.463_56.10R yeast induced significant mortality of field strain Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus larvae in semi-field larvicide trials conducted outdoors in St. Augustine, Trinidad, where 100% of the larvae were dead within 24 h. The yeast was also stably suspended in commercial bait and deployed as an active ingredient in miniature attractive targeted sugar bait (ATSB) station sachets. The yeast ATSB induced high levels of Aedes and Culex mosquito morbidity in semi-field trials conducted in Trinidad, West Indies, as well as in Bangkok, Thailand, in which the consumption of the yeast resulted in adult female mosquito death within 48 h, faster than what was observed in laboratory trials. These findings support the pursuit of large-scale field trials to further evaluate the Sh.463_56.10R insecticide, a member of a promising new class of species-specific RNAi insecticides that could help combat insecticide resistance and support effective mosquito control programs worldwide.
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BACKGROUND: Rabbits are popular pets, but research into their welfare is limited. This study analysed the potential association(s) between the provision of suitable housing and owner demographic factors. METHODS: Data from 2017, 2018 and 2019 PDSA Animal Wellbeing (PAW) report surveys gave a sample of 1333 UK rabbit owners. Whether the rabbit housing was adequate or inadequate was ascertained by asking owners to indicate images of hutch and run size or indoor environment type that were similar to their own. The owner demographic factors collected included gender, education, household income and deprivation. Chi-squared tests and binary logistic regression (univariable and multivariable) were used to investigate factors associated with adequate/inadequate housing. RESULTS: One-third (31.2%) of rabbits lived in inadequate housing and half were housed alone (51.4%). Male owners were more likely to report providing inadequate housing than females (odds ratio [OR] = 1.795, 95% confidence interval [CI] 1.319-2.441, p < 0.001). Compared to owners over 55 years old, those aged 25-34 years were more likely to house their rabbits inadequately (OR = 2.050, 95% CI 1.286-3.267, p = 0.003). Owners with a household income below average were more likely to report providing inadequate housing compared to those with a household income above average (OR = 1.406, 95% CI 1.025-1.928, p = 0.035). CONCLUSION: The findings identify that inadequate rabbit housing is provided by owners of all ages, genders and deprivation levels but highlight some particular owner demographics that could be useful to target if resources are limited.
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Bem-Estar do Animal , Abrigo para Animais/normas , Animais de Estimação/fisiologia , Coelhos/fisiologia , Adulto , Animais , Distribuição de Qui-Quadrado , Intervalos de Confiança , Demografia , Escolaridade , Feminino , Humanos , Renda , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Propriedade , Análise de Regressão , Inquéritos e Questionários , Reino UnidoRESUMO
Dog aggression is a public health concern because dog bites often lead to physical and psychological trauma in humans. It is also a welfare concern for dogs. To prevent aggressive behaviours, it is important to understand human behaviour towards dogs and our ability to interpret signs of dog aggression. This poses ethical challenges for humans and dogs. The aim of this study was to introduce, describe and pilot test a virtual reality dog model (DAVE (Dog Assisted Virtual Environment)). The Labrador model has two different modes displaying aggressive and non-reactive non-aggressive behaviours. The aggressive behaviours displayed are based on the current understanding of canine ethology and expert feedback. The objective of the study was to test the recognition of dog behaviour and associated human approach and avoidance behaviour. Sixteen university students were recruited via an online survey to participate in a practical study, and randomly allocated to two experimental conditions, an aggressive followed by a non-reactive virtual reality model (group AN) or vice versa (group NA). Participants were instructed to 'explore the area' in each condition, followed by a survey. A Wilcoxon and Mann Whitney U test was used to compare the closest distance to the dog within and between groups respectively. Participants moved overall significantly closer to the non-reactive dog compared to the aggressive dog (p≤0.001; r = 0.8). Descriptions of the aggressive dog given by participants often used motivational or emotional terms. There was little evidence of simulator sickness and presence scores were high indicating sufficient immersion in the virtual environment. Participants appeared to perceive the dog as realistic and behaved and interacted with the dog model in a manner that might be expected during an interaction with a live dog. This study also highlights the promising results for the potential future use of virtual reality in behavioural research (i.e., human-dog interactions), education (i.e. safety around dogs) and psychological treatment (e.g. dog phobia treatment).
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Mordeduras e Picadas , Realidade Virtual , Agressão/psicologia , Animais , Cães , Humanos , Projetos Piloto , Inquéritos e QuestionáriosRESUMO
Dog bite injuries are a significant public health problem and many are sustained by children. These injuries can be complex, both physically and psychologically, and in rare cases fatal. This paper will review current evidence-based approaches to treatment, explore identified patterns in biting incidents and discuss the effectiveness of prevention strategies. Safe management of these patients requires a comprehensive approach. Physical injuries need to be accurately assessed with a high index of suspicion for underlying injuries, particularly in younger children less able to communicate. Treatment depends on severity and location, but all bites must be irrigated to reduce the risk of infection but may not always require prophylactic antibiotic use. Careful exploration of the circumstances in which the bite occurred is essential to make safeguarding decisions and prevent future bites. Reducing the incidence of paediatric dog bites requires education of both children and parents that any dog can bite, regardless of breed, and all child-dog interactions must be highly supervised. However, education alone is unlikely to prevent dog bites. Policies that support environmental changes need to be developed such as provision of pet dogs less likely to bite (or bite as severely), through breeding for temperament and appropriate socialisation. Additionally, investment in psychological support for bite victims and their families is required to reduce the long-term impacts of being bitten.
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Domesticated rabbits typically exhibit shorter, flatter skulls than their wild counterparts (brachycephalism). However, brachycephaly is associated with considerable health problems, including problems with dentition. The aim of this study was to establish which type of rabbit face people prefer, with a particular emphasis on skull morphology and brachycephaly. We grouped 25 images of rabbit faces by cephalic degree based on ratings assigned by 134 veterinary professionals. An online questionnaire was then launched, in which people could rate each of the 25 images according to preference for the rabbits' faces, and a total of 20,858 questionnaires were completed globally. Repeated-measure, multi-level general linear modelling revealed mildly-brachycephalic rabbits to be the most preferred type of rabbit, and moderately-dolichocephalic (longer skulled) rabbits to be the least preferred. The preference for brachycephalic rabbits was stable across continents, and as such it is highly plausible that human preference has been a driver for the shortening of the skull typically seen in domestic rabbits, perhaps as a result of the 'baby-schema'. Additional features of rabbit faces that were preferred include a soft, medium-light fur appearance and being generally short-furred. These novel insights may prove useful in the improvement of the public understanding of rabbit health and welfare. The relationship between preference and skull shape is particularly pertinent to future work concerning rabbit health, given the cross-species evidence that having a flat face is associated with chronic health conditions.
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Rabbits are commonly kept in a variety of settings, including homes, laboratories, and veterinary clinics. Despite the popularity of keeping this prey species, little research has investigated current methods of handling. The aim of this study was to examine the experience of caregivers (owners and keepers) in using five handling methods commonly referred to in books written for companion animal (pet) owners and veterinary and/or laboratory personnel. An online survey was completed by 2644 respondents, representing all three of these groups, and breeders. Data were acquired to determine sources that participants used to gain knowledge of different handling methods, the methods they used and for what purposes they used them, and their perceptions of any associated difficulties or welfare concerns. Results indicated that participants most frequently used the method of supporting a rabbit's body against a person's chest, which was considered the easiest and most welfare-friendly method of the handling methods explored. "Scruffing with rear support" was the least used method and was considered to be distressing and painful for the rabbit. As rabbits are a terrestrial prey species, being picked up is likely an innately stressful experience. Additional research is encouraged to explore the experience of rabbits during handling to identify methods that can be easily used with the fewest welfare compromises.
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Criação de Animais Domésticos/métodos , Bem-Estar do Animal , Coelhos , Adolescente , Adulto , Animais , Feminino , Humanos , Irlanda , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Reino UnidoRESUMO
Domestic guinea pigs suffer morbidity and mortality due to a range of bacterial infections amongst other causes. Microorganisms such as Bordetella bronchiseptica and Streptococcus pneumoniae are commonly implicated in respiratory disease; however, there is a lack of research surrounding the prevalence of these bacterial infections. The aim of this study was to investigate the frequency with which owners reported bacterial infections in pet guinea pigs and to assess owner knowledge of correct husbandry practices to inform prevention of the development of bacterial infections. An online questionnaire, consisting of 30 questions was promoted to guinea pig owners. Of all respondents (n = 524), 39.39% reported that their guinea pig(s) had been clinically diagnosed with a bacterial infection, with upper respiratory tract infections the most commonly reported (46.95%), followed by urinary tract (15.49%) and then gastrointestinal infections (11.73%). Owners demonstrated knowledge of correct husbandry practices and there was no significant effect (p = 0.475) of owner knowledge on having owned a guinea pig with a bacterial infection. Bacterial infections may be more common in guinea pigs than was previously thought. Further research is required to understand why bacterial infections are common in pet guinea pigs and to help owners to prevent and recognize these infections.
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Although dogs routinely travel in motor vehicles, there is a lack of evidence on if, how, and why people choose to restrain their dogs when travelling. A lack of restraint is likely to be associated with an increased risk of serious injury or death in the case of an accident, and in some cases may even precipitate an accident. The aim of the present study was to determine the frequency in which dog restraints are used in the US, UK and Australia in a convenience sample, and the factors associated with whether or not a dog is restrained. Online surveys using SurveyMonkey® were distributed in the US, UK and Australia during 2017-2018. The survey consisted of questions related to owning a dog, owner and dog demographics, use of restraint when driving with the dog, reasons for restraining/not restraining the dog, and attitudes to restraint of dogs in vehicles. A logistic regression was used to determine factors associated with the use of restraint. There were 706, 692 and 637 completed surveys from the US, UK and Australia, respectively. A little over half of respondents restrained their dog in the US (55%) compared to 67% in Australia and 72% in the UK. The most common method of restraint in the US and UK was a cage/crate in the cargo area in the back of the vehicle; in Australia it was a harness and tether attached to a seat buckle. In the generalised linear model, country, dog size, owner age, dog age and vehicle type were all significant factors associated with the use of restraint for dogs in cars. Younger dog owners from the US who drove a pickup truck or utility van, had a large dog, and drove with their dogs less frequently were least likely to restrain their dogs. This research highlights the need for improved education and information regarding the use of restraints for dogs traveling in vehicles, although the limitations in the convenience sample used mean further research is needed, including use of a more representative sample.
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Cães , Veículos Automotores/normas , Equipamentos de Proteção/estatística & dados numéricos , Adulto , Idoso , Animais , Austrália , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Veículos Automotores/classificação , Reino Unido , Estados Unidos , Adulto JovemRESUMO
Dog aggression directed towards humans is a common and serious behavioral and public health issue. This cross-sectional study was designed to gain insights into U.S. small animal veterinarians' views and experiences with the most common dog breeds in the U.S., dog aggression, and breed-specific legislation. An electronic survey was distributed via email to an online veterinary community, and responses were summarized and compared by means of χ2 and Cochran-Mantel-Haenszel tests. Most respondents were concerned about the risks of dog bite injuries, but the majority were not in favor of banning specific breeds of dogs in order to enhance public safety. When participants rated the perceived bite risk associated with popular dog breeds, Chow Chows were perceived as the highest risk, with pit bull types categorized as a moderate risk. Golden Retrievers were seen as the most appropriate for families with children. Public education about animal behavior was the most frequently endorsed policy intervention to increase public safety. These findings suggest that most veterinarians feel that banning an entire dog breed is not an effective way to ensure human safety. Instead, most respondents endorsed alternative initiatives, such as public education and stricter leash laws, to reduce the risk of dog bites.
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Agressão , Atitude do Pessoal de Saúde , Comportamento Animal , Cruzamento , Cães/psicologia , Segurança/legislação & jurisprudência , Médicos Veterinários , Adulto , Animais , Mordeduras e Picadas/etiologia , Mordeduras e Picadas/prevenção & controle , Estudos Transversais , Feminino , Pesquisas sobre Atenção à Saúde , Política de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Saúde Pública , Estados UnidosRESUMO
Micronutrient deficiencies affect up to 2 billion people and are the leading cause of cognitive and physical disorders in the developing world. Food fortification is effective in treating micronutrient deficiencies; however, its global implementation has been limited by technical challenges in maintaining micronutrient stability during cooking and storage. We hypothesized that polymer-based encapsulation could address this and facilitate micronutrient absorption. We identified poly(butylmethacrylate-co-(2-dimethylaminoethyl)methacrylate-co-methylmethacrylate) (1:2:1) (BMC) as a material with proven safety, offering stability in boiling water, rapid dissolution in gastric acid, and the ability to encapsulate distinct micronutrients. We encapsulated 11 micronutrients (iron; iodine; zinc; and vitamins A, B2, niacin, biotin, folic acid, B12, C, and D) and co-encapsulated up to 4 micronutrients. Encapsulation improved micronutrient stability against heat, light, moisture, and oxidation. Rodent studies confirmed rapid micronutrient release in the stomach and intestinal absorption. Bioavailability of iron from microparticles, compared to free iron, was lower in an initial human study. An organotypic human intestinal model revealed that increased iron loading and decreased polymer content would improve absorption. Using process development approaches capable of kilogram-scale synthesis, we increased iron loading more than 30-fold. Scaled batches tested in a follow-up human study exhibited up to 89% relative iron bioavailability compared to free iron. Collectively, these studies describe a broad approach for clinical translation of a heat-stable ingestible micronutrient delivery platform with the potential to improve micronutrient deficiency in the developing world. These approaches could potentially be applied toward clinical translation of other materials, such as natural polymers, for encapsulation and oral delivery of micronutrients.
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Temperatura Alta , Micronutrientes/administração & dosagem , Microesferas , Administração Oral , Animais , Disponibilidade Biológica , Transporte Biológico , Preparações de Ação Retardada , Liberação Controlada de Fármacos , Feminino , Humanos , Ácido Hialurônico/química , Absorção Intestinal , Intestinos/fisiologia , Ferro/metabolismo , Metacrilatos/química , Camundongos , Oxirredução , Raios Ultravioleta , Vitamina A/metabolismo , ÁguaRESUMO
The internet has been found to be a popular source for human health information. However, there is a lack of information on pet owners' use of the internet to source pet health information and implications for the owner-veterinarian relationship. Therefore, the aim of this study was to address this gap in knowledge by focusing on UK pet owners' general use of the internet to find online pet health information and the impact of this behaviour on the owner-veterinarian relationship. An online survey targeting UK pet owners resulted in 571 respondents. Respondents reported the most frequently used source for pet health information was the internet (78.6 per cent), followed by their veterinarian (72 per cent). Veterinarians and other pet owners, however, were rated as the most trustworthy sources. The topics searched for most often online were specific medical problems (61.3 per cent) and diet/nutrition (58.5 per cent). Regarding the owner-veterinarian relationship, 42.1 per cent of participants reported discussing information they found online 'sometimes' with their veterinarian. When asked if their veterinarian recommended specific websites, nearly half (49.6 per cent) stated that their veterinarian 'never' made such recommendations, yet over 90 per cent said they would visit veterinarian-recommended websites.
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Informação de Saúde ao Consumidor , Internet/estatística & dados numéricos , Animais de Estimação , Adulto , Animais , Feminino , Humanos , Masculino , Propriedade , Relações Profissional-Paciente , Reino Unido , Médicos Veterinários/psicologiaRESUMO
Many dog owners allow their pets to jump out of a car boot; however, to date, there has been no study that has investigated whether this places dogs at risk of injury. The aim of this study was to investigate the relationship between height and peak vertical ground reaction force (vGRF) in static start jumps. Fifteen healthy adult dogs performed three jumps from a platform that represented common vehicle boot sill heights (0.55, 0.65, 0.75 m), landing on a single force platform. Kinetic data (mediolateral (Fx), craniocaudal (Fy) and vertical (Fz)) were normalised for body weight and analysed via a one-way repeated analysis of variance (ANOVA) and pairwise post hoc tests with a Bonferroni correction applied. There was a significant difference in peak forelimb vGRF between both the 0.55 m (27.35±4.14 N/kg) and the 0.65 m (30.84±3.66 N/kg) platform (P=0.001) and between the 0.65 and 0.75 m (34.12±3.63 N/kg) platform (P=0.001). There was no significant difference in mediolateral or craniocaudal forces between the heights examined. These results suggest that allowing dogs to jump from bigger cars with a higher boot sill may result in augmented levels of loading on anatomical structures. Further research is required to investigate the kinematic effects of height on static jump-down and how peak forelimb vGRF relates to anatomical loading and subsequent injury risk.
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Cães/fisiologia , Membro Anterior/fisiologia , Movimento , Animais , Automóveis , Fenômenos Biomecânicos , CinéticaRESUMO
The Internet is a commonly used resource for accessing health information. Despite the Internet's popularity in the human health field, little is known about the Internet's impact on veterinarians, their clients, and the veterinarian-client relationship. The aim of this study was to investigate the perception of veterinarians from the United Kingdom of clients' use of the Internet and the perceived impact on pet health and the veterinarian-client relationship. A survey was distributed between January 4 and March 3, 2017, via an online link. In total, 100 veterinarians completed the survey. This study found that most UK veterinarians feel their clients access the Internet to find pet health information, yet often do not understand what they read online. Importantly, 40% of veterinarians stated that the Internet has a negative impact on companion animal health. This small-scale study found mixed opinions regarding veterinarians' perceptions of their clients' use of the Internet and the potential impact it has on the client-veterinarian relationship. Research on clients' actual use of the Internet and their associated perceptions is a next logical step.
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Source types or source regions contributing to the concentration of atmospheric fine particles measured at Brigantine National Wildlife Refuge, NJ, were identified using a factor analysis model called Positive Matrix Factorization (PMF). Cluster analysis of backward air trajectories on days of high- and low-factor concentrations was used to link factors to potential source regions. Brigantine is a Class I visibility area with few local sources in the center of the eastern urban corridor and is therefore a good location to study Mid-Atlantic regional aerosol. Sulfate (expressed as ammonium sulfate) was the most abundant species, accounting for 49% of annual average fine mass. Organic compounds (22%; expressed as 1.4 x organic carbon) and ammonium nitrate (10%) were the next abundant species. Some evidence herein suggests that secondary organic aerosol formation is an important contributor to summertime regional aerosol. Nine factors were identified that contributed to PM2.5 mass concentrations: coal combustion factors (66%, summer and winter), sea salt factors (9%, fresh and aged), motor vehicle/mixed combustion (8%), diesel/Zn-Pb (6%), incinerator/industrial (5%), oil combustion (4%), and soil (2%). The aged sea salt concentrations were highest in springtime, when the land breeze-sea breeze cycle is strongest. Comparison of backward air trajectories of high- and low-concentration days suggests that Brigantine is surrounded by sources of oil combustion, motor vehicle/mixed combustion, and waste incinerator/industrial emissions that together account for 17% of PM2.5 mass. The diesel/Zn-Pb factor was associated with sources north and west of Brigantine. Coal combustion factors were associated with coal-fired power plants west and southwest of the site. Particulate carbon was associated not only with oil combustion, motor vehicle/mixed combustion, waste incinerator/industrial, and diesel/Pb-Zn, but also with the coal combustion factors, perhaps through common transport.