RESUMO
ß-D-glucans are a (1â3)-linked glucose polymer with (1â6)-linked side chains and a major component of fungal cell walls. They exhibit structural integrity to the fungal cell wall. In addition, ß-glucans are widely used as food adjuvant in food and pharmaceutical industries because of their physico-chemical properties. Several studies have focused on different isolation processes of (1â3) (1â6)-ß-glucan that could affect the physico-chemical and functional properties of ß-glucan such as chemical composition, solubility, viscosity, hydration properties, and oil binding capacity. Immunological activity is one of the most important properties of ß-glucans. Thus, they are effective in inhibiting growth of cancer cells and metastasis and preventing bacterial infection. In humans, ß-glucans reduce blood cholesterol, improve glucose absorption by body cells, and so help wound healing. This review described the prebiotic potentiality of fungal ß-D-glucans with the objective to detail the methodologies applied for their extraction, their structure and techno-functional properties, and finally their biological effects.
Assuntos
Fungos/química , beta-Glucanas/química , Animais , Anti-Infecciosos/química , Antineoplásicos/química , Antioxidantes/análise , Pressão Sanguínea/efeitos dos fármacos , Aditivos Alimentares/análise , Humanos , Hipoglicemiantes/química , Imunomodulação , Prebióticos , Solubilidade , Viscosidade , Cicatrização/efeitos dos fármacos , beta-Glucanas/isolamento & purificaçãoRESUMO
BACKGROUND: Ferulic acid esterified with poly(ethylene glycol) with three different average molecular weights (200, 400 and 1000 g mol(-1)) was studied in bread-making. The effects of these antioxidants on the properties of wheat flour dough and bread were analysed and compared with those obtained with ferulic acid and two commercial surfactants, the diacetyl tartaric acid ester of mono- and diglycerides and sodium stearoyl lactylate. Farinographic and alveographic methods as well as weight, volume and bread firmness measurements were used for this purpose. RESULTS: Similar to ferulic acid, when the PEGylated derivatives were implemented in the dough (5000 ppm), it accelerated the breakdown of the dough and decreased its rheological properties. However, the important diminution of loaf volume, observed when dough supplemented with ferulic acid was baked, was avoided. That decrease in volume was related to the inhibition of the yeast (Saccharomyces cerevisae) by the unesterified ferulic acid. Moreover, two of the PEGylated ferulic acids even contributed to an increase of loaf volumes (5-6%) and demonstrated crumb softener properties. CONCLUSION: The addition of ferulic acid to wheat flour dough caused the inhibition of the yeast, which resulted in decreased bread volume. That effect could be avoid by the esterification of ferulic acid with poly(ethylene glycol).
Assuntos
Pão/análise , Ácidos Cumáricos , Farinha/análise , Manipulação de Alimentos/métodos , Polietilenoglicóis , Tensoativos , Triticum , Antioxidantes , Esterificação , Microbiologia de Alimentos , Qualidade dos Alimentos , Humanos , Reologia , SaccharomycesRESUMO
We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles-midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 ml g⻹ dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 ml g⻹, respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media. The bioconversion yields for each MP were below 50 %, showing that these substrates were not fully biodegraded after 188 days. The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation-Del Monte plantations for 188 days is approximately 10.5 × 106 kW h (which would be worth 0.80-1.58 million euros in the current market). This bioenergy could serve the requirements of about 42,000 people in the region, although CH4 productivity could be improved.
Assuntos
Biocombustíveis/análise , Ácidos Graxos/química , Lignina/química , Musa/metabolismo , Estruturas Vegetais/química , Acetatos/metabolismo , Anaerobiose , Biodegradação Ambiental , Biomassa , Carbono/metabolismo , Cromatografia Líquida , Eletricidade , Flores/química , Hidróxidos/metabolismo , Musa/química , Nitrogênio/metabolismo , Folhas de Planta/química , Raízes de Plantas/química , Caules de Planta/química , Compostos de Potássio/metabolismoRESUMO
Four co-solvents (dimethylformamide [DMF], formamide, dimethyl sulfoxide [DMSO], and pyridine) were tested with tert-butanol (tBut) to optimize the initial rate (v0) and yield of mannosyl myristate synthesis by esterification catalyzed by immobilized lipase B from Candida antarctica. Ten percent by volume of DMSO resulted in the best improvement of v0 and 48-hr yield (respectively 115% and 13% relative gain compared to pure tBut). Use of molecular sieve (5% w/v) enhances the 48-hr yield (55% in tBut/DMSO [9:1, v/v]). Transesterification in tBut/DMSO (9:1, v/v) with vinyl myristate leads to further improvement of v0 and 48-hr yield: a relative gain of 85% and 65%, respectively, without sieve and 25% and 10%, respectively, with sieve, compared to esterification. No difference in v0 and 48-hr yield is observed when transesterification is carried out with or without sieve.
Assuntos
Candida/enzimologia , Enzimas Imobilizadas/metabolismo , Lipase/metabolismo , Manose/metabolismo , Ácido Mirístico/metabolismo , Biotecnologia/métodos , Candida/metabolismo , Enzimas Imobilizadas/química , Esterificação , Ácidos Graxos/metabolismo , Lipase/química , Manose/análogos & derivados , Desnaturação Proteica , Solventes/químicaRESUMO
This critical review article presents the current state of knowledge on isomalto-oligosaccharides, some well known functional oligosaccharides in Asia, to evaluate their potential as emergent prebiotics in the American and European functional food market. It includes first a unique inventory of the different families of compounds which have been considered as IMOs and their specific structure. A description has been given of the different production methods including the involved enzymes and their specific activities, the substrates, and the types of IMOs produced. Considering the structural complexity of IMO products, specific characterization methods are described, as well as purification methods which enable the body to get rid of digestible oligosaccharides. Finally, an extensive review of their techno-functional and nutritional properties enables placing IMOs inside the growing prebiotic market. This review is of particular interest considering that IMO commercialization in America and Europe is a topical subject due to the recent submission by Bioneutra Inc. (Canada) of a novel food file to the UK Food Standards Agency, as well as several patents for IMO production.
Assuntos
Alimento Funcional , Conhecimentos, Atitudes e Prática em Saúde , Oligossacarídeos/química , Prebióticos , América , Animais , Anticarcinógenos/farmacologia , Ásia , Canadá , Europa (Continente) , Humanos , Fatores Imunológicos/farmacologia , Oligossacarídeos/farmacologiaRESUMO
By screening uronic acid-based surfactant interfacial properties, the effect of the hydroxyl group stereochemistry (OH-4) on the conformation of bicatenary (disubstituted) derivatives at the air-water interface has been evidenced by experimental and computational approaches. Physical and optical properties of a monolayer characterized by Langmuir film balance, Brewster angle microscopy, and ellipsometry at 20 °C reveal that the derivative of glucuronate (C(14/14)-GlcA) forms a more expanded monolayer, and shows a transition state under compression, in the opposite to that of galacturonate (C(14/14)-GalA). Both films are very mechanically resistant (compression modulus > 300 mN m(-1)) and stable (collapse pressure exceeding 60 mN m(-1)), while that of C(14/14)-GalA exhibits a very high compression modulus up to 600 mN m(-1) like films in the solid state. Computational approaches provide single and assembly molecular models that corroborate the molecule expansion degree and interactions data from experimental results. Differences in the molecular conformation and film behaviours of uronic acid bicatenary derivatives at the air-water interface are attributed to the intra-H-bonding formation, which is more favourable with an OH-4 in the axial (C(14/14)-GalA) than in the equatorial position (C(14/14)-GlcA).
Assuntos
Hidróxidos/química , Simulação de Dinâmica Molecular , Tensoativos/química , Ácidos Urônicos/química , Ar , Modelos Moleculares , Estrutura Molecular , Pressão , Estereoisomerismo , Propriedades de Superfície , Água/químicaRESUMO
Musa sp. peels are widely used by smallholders as complementary feeds for cattle in the tropics. A study of the influence of the variety and the maturation stage of the fruit on fermentability and metabolisable energy (ME) content of the peels was performed using banana (Yangambi Km5) and plantain (Big Ebanga) peels at three stages of maturation in an in vitro model of the rumen. Peel samples were analysed for starch, free sugars and fibre composition. Samples were incubated in the presence of rumen fluid. Kinetics of gas production were modelled, ME content was calculated using prediction equation and short-chain fatty acids production and molar ratio were measured after 72 h of fermentation. Final gas production was higher in plantain (269-339 ml g(-1)) compared to banana (237-328 ml g(-1)) and plantain exhibited higher ME contents (8.9-9.7 MJ/kg of dry matter, DM) compared to banana (7.7-8.8 MJ/kg of DM). Butyrate molar ratio decreased with maturity of the peels. The main influence of the variety and the stage of maturation on all fermentation parameters as well as ME contents of the peels was correlated to changes in the carbohydrate fraction of the peels, including starch and fibre.
Assuntos
Ração Animal/análise , Frutas/química , Musa/química , Camarões , Cromatografia Líquida de Alta Pressão , Fibras na Dieta/análise , Sacarose Alimentar/análise , Fermentação , Frutas/microbiologia , Cinética , Modelos Lineares , Metano/biossíntese , Musa/microbiologia , Amido/análiseRESUMO
We used real-time atomic force microscopy (AFM) to visualize the interactions between supported lipid membranes and well-defined surfactin analogs, with the aim to understand the influence of geometry, charge and hydrophobicity. AFM images of mixed dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers recorded after injection of cyclic surfactin at 1 mM, i.e. well-above the critical micelle concentration, revealed a complete solubilization of the bilayers within 30 min. A linear analog having the same charge and acyl chains was able to solubilize DOPC, but not DPPC, and to promote redeposition leading eventually to a new bilayer. Increasing the charge of the polar head or the length of the acyl chains of the analogs lead to the complete solubilization of both DOPC and DPPC, thus to a stronger membrane activity. Lastly, we found that at low surfactin concentrations (40 microM), DPPC domains were always resistant to solubilization. These data demonstrate the crucial role played by geometry, charge and hydrophobicity in modulating the membrane activity (solubilization, redeposition) of surfactin. Also, this study suggests that synthetic analogs are excellent candidates for developing new surfactants with tunable, well-defined properties for medical and biotechnological applications.
Assuntos
Bicamadas Lipídicas/química , Lipopeptídeos/química , Peptídeos Cíclicos/química , Sequência de Aminoácidos , Lipopeptídeos/genética , Lipídeos de Membrana/química , Microscopia de Força Atômica , Peptídeos Cíclicos/genética , Fosfatidilcolinas/química , Propriedades de SuperfícieRESUMO
Interfacial properties of surfactants are dependent on the conformation adopted by the hydrophilic headgroup or/and the hydrophobic tail at the boundary limit of two immiscible phases. Here, we demonstrate the impacts of the carbonyl group (-CO-) location of the ester bond of sugar-based surfactants by comparing some properties of two closely related esters, octyl glucuronate and glucose octanoate, at the air-water interface. The carbonyl group location influences the rate and extent of interfacial adsorption and the rheology properties of sugar esters at the air-water interface, which were evaluated by dynamic surface tension and complex surface viscoelastic measurements. Octyl glucuronate adsorbs the fastest at the air-water interface whereas glucose octanoate reduces the dynamic surface tension at the lowest value and exhibits the highest film viscoelasticity. Differences are attributed to molecular conformation constraints inducing relevant changes to the surface coverage kinetic capacity and the interaction strengths of the octyl sugar ester adsorbed films at the air-water interface. All of the results are supported by the minimum cross-sectional area values per molecule determined by both experimental and computational approaches.
Assuntos
Carboidratos/química , Ésteres/química , Modelos Moleculares , Tensoativos/química , Ar , Configuração de Carboidratos , Simulação por Computador , Glucose/química , Glucuronatos/química , Peso Molecular , Reologia , Tensão Superficial , Fatores de Tempo , Substâncias Viscoelásticas/química , Água/químicaRESUMO
Fengycin is a biologically active lipopeptide produced by several Bacillus subtilis strains. The lipopeptide is known to develop antifungal activity against filamentous fungi and to have hemolytic activity 40-fold lower than that of surfactin, another lipopeptide produced by B. subtilis. The aim of this work is to use complementary biophysical techniques to reveal the mechanism of membrane perturbation by fengycin. These include: 1), the Langmuir trough technique in combination with Brewster angle microscopy to study the lipopeptide penetration into monolayers; 2), ellipsometry to investigate the adsorption of fengycin onto supported lipid bilayers; 3), differential scanning calorimetry to determine the thermotropic properties of lipid bilayers in the presence of fengycin; and 4), cryogenic transmission electron microscopy, which provides information on the structural organization of the lipid/lipopeptide system. From these experiments, the mechanism of fengycin action appears to be based on a two-state transition controlled by the lipopeptide concentration. One state is the monomeric, not deeply anchored and nonperturbing lipopeptide, and the other state is a buried, aggregated form, which is responsible for membrane leakage and bioactivity. The mechanism, thus, appears to be driven mainly by the physicochemical properties of the lipopeptide, i.e., its amphiphilic character and affinity for lipid bilayers.
Assuntos
Bacillus subtilis/metabolismo , Bicamadas Lipídicas/química , Fluidez de Membrana , Modelos Químicos , Modelos Moleculares , Fosfolipídeos/química , Simulação por Computador , Lipopeptídeos , Lipoproteínas/química , Lipossomos/química , Membranas Artificiais , Conformação MolecularRESUMO
Lipozyme TL IM was used in a solvent-free batch and microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ternary blend. The aim was to obtain and characterize physicochemically fats enriched with unsaturated C 18 fatty acids (oleic, linoleic, and, especially, linolenic acids) from natural vegetable oils. Binary blends of AMF/LO 100/0, 90/10, 80/20, 70/30, and 60/40 (w/w) were interesterified. The change in triacylglycerol (TAG) profiles showed that quasi-equilibrium conditions were reached after 4-6 h of reaction. Free fatty acid contents <1%. The decrease in solid fat content and in dropping point temperature obtained with increasing content of LO and interesterification resulted in good plastic properties for the products originating from the blends 70/30 and 60/40. This was confirmed by textural measurements. Melting profiles determined by differential scanning calorimetry showed complete disappearance of low-melting TAGs from LO and the formation of intermediary species with a lower melting temperature. Oxidative stability of the interesterified products was diminished with increasing LO content, resulting in low oxidation induction times. A ternary blend composed of AMF/RO/LO 70/20/10 gave satisfactory rheological and oxidative properties, fulfilling the requirements for a marketable spread and, moreover, offering increased potential health benefits due to the enriched content in polyunsaturated fatty acid residues.
Assuntos
Ácidos Graxos Insaturados/análise , Óleo de Semente do Linho/química , Lipase/metabolismo , Leite/química , Óleos de Plantas/química , Triglicerídeos/metabolismo , Animais , Varredura Diferencial de Calorimetria , Esterificação , Ácidos Graxos Monoinsaturados , Humanos , Oxirredução , Óleo de Brassica napus , Reologia , Temperatura , Triglicerídeos/análiseRESUMO
The effects of the ripeness stage of banana (Musa AAA) and plantain (Musa AAB) peels on neutral detergent fibre, acid detergent fibre, cellulose, hemicelluloses, lignin, pectin contents, and pectin chemical features were studied. Plantain peels contained a higher amount of lignin but had a lower hemicellulose content than banana peels. A sequential extraction of pectins showed that acid extraction was the most efficient to isolate banana peel pectins, whereas an ammonium oxalate extraction was more appropriate for plantain peels. In all the stages of maturation, the pectin content in banana peels was higher compared to plantain peels. Moreover, the galacturonic acid and methoxy group contents in banana peels were higher than in plantain peels. The average molecular weights of the extracted pectins were in the range of 132.6-573.8 kDa and were not dependant on peel variety, while the stage of maturation did not affect the dietary fibre yields and the composition in pectic polysaccharides in a consistent manner. This study has showed that banana peels are a potential source of dietary fibres and pectins.
Assuntos
Biotecnologia/métodos , Fibras na Dieta , Musa/metabolismo , Pectinas/análise , Plantago/metabolismo , Carboidratos , Celulose/química , Ácidos Hexurônicos/análise , Íons , Metilação , Peso Molecular , Oxalatos/análise , Polissacarídeos/análise , Especificidade da Espécie , TemperaturaRESUMO
The influence of harvest date on pectin quality and yield of five varieties of chicory is investigated in this work. The pectin is extracted from the raw material with an acidic treatment at 85°C for an hour after inulin extraction. Main changes due to harvest date are observed in terms of neutral sugar content, average molecular weight, esterification degree and extraction yield. The galacturonic acid content remains relatively constant throughout the harvest period. While no statistically significant differences (p<0.05) in terms of galacturonic acid content and neutral sugar content are observed between the five cultivars studied, average molecular weight, esterification degree and extraction yield are significantly affected by the cultivar type. Depending on the harvest date, a broad range of pectins can easily be extracted from chicory pulps.
RESUMO
An experimental design was used to study the influence of pH (1.5 and 2.0), temperature (80 and 90°C) and time (1 and 4h) on extraction of pectin from banana peels (Musa AAA). Yield of extracted pectins, their composition (neutral sugars, galacturonic acid, and degree of esterification) and some macromolecular characteristics (average molecular weight, intrinsic viscosity) were determined. It was found that extraction pH was the most important parameter influencing yield and pectin chemical composition. Lower pH values negatively affected the galacturonic acid content of pectin, but increased the pectin yield. The values of degree of methylation decreased significantly with increasing temperature and time of extraction. The average molecular weight ranged widely from 87 to 248kDa and was mainly influenced by pH and extraction time.
RESUMO
Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopeptides have a major role in the antagonism of B. subtilis toward P. fusca.
Assuntos
Ascomicetos/fisiologia , Bacillus subtilis/fisiologia , Peptídeos Cíclicos/fisiologia , Antibiose , Ascomicetos/efeitos dos fármacos , Bacillus subtilis/química , Bacillus subtilis/genética , Cucurbitaceae/microbiologia , Lipopeptídeos , Lipoproteínas/química , Lipoproteínas/isolamento & purificação , Lipoproteínas/farmacologia , Lipoproteínas/fisiologia , Mutagênese Sítio-Dirigida , Peptídeos Cíclicos/química , Peptídeos Cíclicos/isolamento & purificação , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Espanha , Transformação BacterianaRESUMO
The surface chemical composition of nine strains of Bacillus subtilis was determined by X-ray photoelectron spectroscopy. Regressions between elemental concentrations and concentrations associated with different components of C1s, N1s, and O1s peaks provided a more precise validation of the procedure used for peak decomposition and allowed the assignment of the peak components to be completed or strengthened. The component of the O1s peak appearing around 531.2 eV was shown to contain a contribution of oxygen from phosphate groups (PO, PO-), the other contribution being due to oxygen involved in amide functions. The surface negative charge may be fully attributed to phosphate groups, despite the observation of two types of zeta potential vs pH curves. The strains exhibiting a sharp variation of the zeta potential (range of -35 to -55 mV) between pH 2 and 4.7 were characterized by a high phosphate surface concentration and by an excess (about 25%) of phosphate with respect to the sum of potassium, an exchangeable cation, and protonated nitrogen, attributed to protein or to alanine involved in teichoic acids.
Assuntos
Bacillus subtilis/química , Elementos Químicos , Concentração de Íons de Hidrogênio , Espectrometria por Raios X , Eletricidade Estática , Propriedades de SuperfícieRESUMO
New linear analogs of surfactin have been synthesized. Their physico-chemical parameters were determined. The results indicate that these linear products show surface activities although they are lowered compared to those of cyclic compounds. The hemolytic activities have also been assayed. In contrast with cyclic surfactins, no significant hemolysis occurs for the linear products in the range of concentrations tested. Moreover, a protective effect against Triton X-100 induced hemolysis has been highlighted for linear surfactins. The concentration at which this protective effect happens is correlated directly to the CMC, and inversely to the acyl chain length of the product. In a hypotonic medium, analogs having a long acyl chain tend to increase the hemolysis, meanwhile the product with the shortest chain tends to decrease it.
Assuntos
Hemólise/efeitos dos fármacos , Peptídeos Cíclicos/química , Tensoativos/química , Cinética , Lipopeptídeos , Octoxinol , Peptídeos Cíclicos/farmacologia , Tensoativos/farmacologiaRESUMO
Chicory root pectin was isolated by acid extraction followed by alcohol precipitation. Because the extraction conditions have important effects on the features of pectins, an experimental design was used to study the influence of 17 different extraction parameters on yield and composition of pectin: pH, temperature, time of extraction, solid/liquid ratio, and different pretreatments of the pulps before extraction. Twenty extractions were conducted and examined for their significance on yield and sugar content using the Plackett-Burman factorial design. The acid extraction of chicory roots resulted in an average yield of 11% containing 86% of sugars. It was found that extraction temperature, time, protease pretreatment, water purity, and water washing of pulps significantly affected yield and pectin composition with an increase of yield and purity of pectin in harsher extraction conditions.
Assuntos
Cichorium intybus/química , Pectinas/química , Pectinas/isolamento & purificação , Raízes de Plantas/química , Precipitação Química , Etanol , Concentração de Íons de Hidrogênio , Peptídeo Hidrolases , Solubilidade , Temperatura , Fatores de TempoRESUMO
The yeast cell wall of Saccharomyces cerevisiae is an important source of ß-d-glucan, a glucose homopolymer with many functional, nutritional and human health benefits. In the present study, the yeast cell wall fractionation process involving enzymatic treatments (savinase and lipolase enzymes) affected most of the physical and functional characteristics of extracted fractions. Thus, the fractionation process showed that ß-d-glucan fraction F4 had significantly higher swelling power and fat binding capacity compared to other fractions (F1, F2 and F3). It also exhibited a viscosity of 652.12mPas and a high degree of brightness of extracted ß-d-glucan fraction. Moreover, the fractionation process seemed to have an effect on structural and thermal properties of extracted fractions. Overall, results showed that yeast ß-d-glucan had good potential for use as a prebiotic ingredient in food, as well as medicinal and pharmaceutical products.
Assuntos
Parede Celular/química , Fracionamento Químico/métodos , Saccharomyces cerevisiae/químicaRESUMO
In this study, we investigated the interaction of fengycin, a lipopeptide produced by Bacillus subtilis, with lipid monolayers using the Langmuir trough technique in combination with Brewster angle microscopy. Thermodynamic analyses were performed to get further information about the mixing behavior and the molecular interactions between the two components. The effect of fengycin on the structural and morphological characteristics of DPPC monolayers, as a simple model of biological membranes, depends on the fengycin molar ratio. With a small proportion of fengycin (X(f)0.1), the compressibility of the monolayer is modified but the morphological characteristics of the DPPC are not significantly affected. At an intermediate molar ratio (0.1Assuntos
Lipídeos de Membrana/química
, Membranas Artificiais
, Ar
, Bacillus subtilis/química
, Lipopeptídeos
, Lipoproteínas/química
, Estrutura Molecular
, Propriedades de Superfície
, Água/química