Infection with the Lyme disease pathogen suppresses innate immunity in mice with diet-induced obesity.

Obesity is a major global public health concern. Immune responses implicated in obesity also control certain infections. We investigated the effects of high-fat diet-induced obesity (DIO) on infection with the Lyme disease bacterium Borrelia burgdorferi in mice. DIO was associated with systemic suppression of neutrophil- and macrophage-based innate immune responses. These included bacterial uptake and cytokine production, and systemic, progressive impairment of bacterial clearance, and increased carditis severity. B. burgdorferi-infected mice fed normal diet also gained weight at the same rate as uninfected mice fed high-fat diet, toll-like receptor 4 deficiency rescued bacterial clearance defects, which greater in female than male mice, and killing of an unrelated bacterium (Escherichia coli) by bone marrow-derived macrophages from obese, B. burgdorferi-infected mice was also affected. Importantly, innate immune suppression increased with infection duration and depended on cooperative and synergistic interactions between DIO and B. burgdorferi infection. Thus, obesity and B. burgdorferi infection cooperatively and progressively suppressed innate immunity in mice.

Comparative evaluation of biodentine and endosequence root repair material as direct pulp capping material: A clinical study.

Aim: The study was designed to clinically evaluate biodentine and endosequence root repair material (ERRM) as direct pulp capping agent at 3 months, 6 months, and 12 months intervals. Materials and Methods: Sixty permanent posterior teeth with deep caries, without any signs of irreversible pulpitis were allotted into two experimental groups: Group I -Biodentine, Group II-ERRM. Follow-up evaluations were done. Every 24 h for 7 days, the pain was assessed using visual analog scale. Statistical Analysis: Paired t-test along with Pearson's Chi-square test was performed. Results: The success rate of clinical assessment at 12 months for biodentine group is 78.60% and ERRM Group is 64.70%. Conclusion: Biodentine group performed superior than ERRM group clinically at all-time interval and hence it can be successfully used to restore exposed vital tooth.

Comparative evaluation of effect of chloroquick irrigating solution on push-out bond strength of endoseal mineral trioxide aggregate and endosequence root repair material when used as furcal perforation repair material: An in vitro study.

AIM: The purpose of this study was to comparatively evaluate the effect of Chloroquick irrigating solution on push-out bond strength of Endoseal mineral trioxide aggregate (MTA) and endosequence root repair material (ERRM) when used as furcal perforation repair materials. MATERIALS AND METHODS: Forty human extracted mandibular molars were collected. A standardized endodontic access cavity was prepared in 40 samples, and intentional perforation of 1.32 mm in diameter was created on the pulpal floor. Teeth were randomly divided into four groups: Group A: EndoSeal MTA with irrigation, Group B: ERRM with irrigation, Group C: EndoSeal MTA without irrigation (Control group), and Group D: ERRM without irrigation (Control group). The samples were subjected to universal testing machine then examined under a stereomicroscope at × 40 to determine the nature of the bond failures. RESULTS: EndoSeal MTA with Chloroquick irrigating solution irrigation showed the highest push-out bond strength (MPa) with a statistically significant difference among all the groups (P = 0.003). The majority of the samples exhibited cohesive and mixed types of failures. CONCLUSION: Chloroquick irrigating solution irrigation has no adverse effect on the push-out dentin bond strength of Endoseal MTA and ERRM.

Comparative evaluation of enamel surface roughness after debonding using four finishing and polishing systems for residual resin removal-an in vitro study.

BACKGROUND: Orthodontic bonding and debonding procedures involve risk of damaging the enamel surface and changing its original morphology. The rough surface inhibits proper cleaning, invites plaque deposition, bacterial retention, and stain formation thus dampening the esthetic appearance of the teeth. Restoring the enamel to its original morphology is a challenge. Researches on better adhesive removal methods which can effectively remove the residual resin and restore it best to its original form are continuing till date. No study has compared four contemporary finishing systems for their efficiency on a single platform. AIM: The objective of this study is to evaluate and compare enamel surface roughness after debonding using four different finishing and polishing systems. MATERIAL AND METHODS: Adhesive resin was removed from the buccal surface of 88 premolars after debonding with 4 groups. It included 22 teeth per group: group 1-One gloss system; group 2-Enhance finishing and polishing system; group 3-fiber reinforced stainbuster bur; and group 4-Soflex discs with wheels. Roughness was measured quantitatively and qualitatively with the help of surface roughness tester and scanning electron Microscope (SEM) respectively. RESULTS: No significant difference was found in baseline roughness in four groups. Highest post-polishing roughness was observed in Soflex group (4.62 µm) followed by One gloss system (3.36 µm), Enhance system (3.17 µm), and stainbuster bur (1.99 µm) (p value < 0.01). CONCLUSION: Stainbuster bur created the smoothest enamel surface that was close to the natural enamel followed by Enhance system, One gloss system, and Soflex disc and wheels.

Efficacy of Removal of Calcium Hydroxide Medicament from Root Canals by Endoactivator and Endovac Irrigation Techniques: A Systematic Review of In vitro Studies.

INTRODUCTION: The aim of this systematic review was to systematically evaluate and summarize the outcomes of in vitro studies comparing Endoactivator irrigation and Endovac irrigation techniques for removing calcium hydroxide (Ca[OH]2) medicament from the root canals. MATERIALS AND METHODS: The research question was developed according to the population, intervention, comparison, and outcome strategy. A computerized literature search was conducted in Medline, PubMed, Google Scholar, and Embase. A hand search of the reference lists of identified articles was performed to separate relevant articles. Two reviewers critically assessed the studies that fulfilled the inclusion criteria and processed. Evaluation of the risk of bias of the studies was performed independently by the two reviewers. RESULTS: After study selection, 61 were assessed for eligibility. Of these, 13 met the inclusion criteria and were included in the systematic review. Since significant heterogeneity was found in the methodologies, it was not possible to conduct a meta-analysis. CONCLUSIONS: On the basis of available evidence, we determined that Endoactivator irrigation technique showed better performance in removing Ca(OH)2 intracanal medicaments from middle third and coronal third area of the root canals and Endovac irrigation technique showed better performance from the apical third area of the root canals. Due to the limitations, small sample sizes, and low number of included studies, further research is needed to confirm our results.

Hyperglycemia Impairs Neutrophil-Mediated Bacterial Clearance in Mice Infected with the Lyme Disease Pathogen.

Insulin-insufficient type 1 diabetes is associated with attenuated bactericidal function of neutrophils, which are key mediators of innate immune responses to microbes as well as pathological inflammatory processes. Neutrophils are central to immune responses to the Lyme pathogen Borrelia burgdorferi. The effect of hyperglycemia on host susceptibility to and outcomes of B. burgdorferi infection has not been examined. The present study investigated the impact of sustained obesity-independent hyperglycemia in mice on bacterial clearance, inflammatory pathology and neutrophil responses to B. burgdorferi. Hyperglycemia was associated with reduced arthritis incidence but more widespread tissue colonization and reduced clearance of bacterial DNA in multiple tissues including brain, heart, liver, lung and knee joint. B. burgdorferi uptake and killing were impaired in neutrophils isolated from hyperglycemic mice. Thus, attenuated neutrophil function in insulin-insufficient hyperglycemia was associated with reduced B. burgdorferi clearance in target organs. These data suggest that investigating the effects of comorbid conditions such as diabetes on outcomes of B. burgdorferi infections in humans may be warranted.