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Methods Mol Biol ; 1224: 37-45, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25416247

RESUMO

Sesame (Sesamum indicum L.) is an important oilseed crop grown in India, China, Korea, Russia, Turkey, Mexico, South America, and several countries of Africa. Sesame seeds are rich in oil, proteins, unsaturated fatty acids, vitamins, minerals, and folic acid. Nearly 70% of the world's sesame is processed into oil and meal, while the remainder is channeled to food and confectionery industries. Production of sesame is limited by several fungal diseases, water logging, salinity, and shattering of seed capsules during harvest. Introgression of useful genes from wild species into cultigens by conventional breeding has not been successful due to postfertilization barriers. The only alternative for the improvement of S. indicum is to transfer genes from other sources through genetic transformation techniques. Here, we describe a simple, fast, and reproducible method for the Agrobacterium-mediated genetic transformation of S. indicum which may be employed for the transfer of desirable traits into this economically important oilseed crop.


Assuntos
Agrobacterium tumefaciens/genética , Engenharia Genética/métodos , Sesamum/crescimento & desenvolvimento , Sesamum/genética , Aclimatação , Agrobacterium tumefaciens/crescimento & desenvolvimento , Técnicas de Cocultura , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas , Sesamum/fisiologia , Fatores de Tempo , Transformação Genética
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