Assuntos
Impressões Digitais de DNA/métodos , Frequência do Gene/genética , Repetições de Microssatélites/genética , Polimorfismo Genético , População Branca/genética , Alelos , Manchas de Sangue , Feminino , Genes Dominantes/genética , Marcadores Genéticos/genética , Genética Populacional , Humanos , Masculino , Polônia , Reação em Cadeia da Polimerase/métodos , Valores de ReferênciaRESUMO
This paper presents the identification of three rare alleles: D21S11*24.2, D7S820*9.1 and HUMFGA*19.2 not observed previously in a Polish population. Allelic variants were identified during routine analysis of more than 1600 reference samples. DNA extracts were amplified using ProfilerPlus kit or monoplex reactions. PCR products were separated using capillary electrophoresis with fluorescent detection. Population genetics data of D21S11, D7S820 and HUMFGA loci including minimal allele frequencies (pmin) in large population samples from Poland are also presented.
Assuntos
Alelos , Eletroforese Capilar/métodos , Frequência do Gene , DNA/sangue , Marcadores Genéticos/genética , Genética Populacional , Humanos , Polônia , Reação em Cadeia da Polimerase/métodos , Sequências de Repetição em Tandem/genéticaRESUMO
The objective of the research was to provide a comprehensive database of autosomal microsatellite loci included in AmpFlSTR NGM PCR kit for a population of Poland considering possible genetic differentiation of a forensic interest. Fifteen STR markers were analyzed in 2041 unrelated individuals residing in eight geographically different regions. All the loci were found to be in Hardy-Weinberg equilibrium. The combined probability of match is 3.52 × 10(-19) and the combined Power of Exclusion is 0.9999998. The F(ST) estimate over all 15 STRs is 0.0051 for the Polish population. We established that a combined NGM database may be employed for a Polish population.