Physical properties of technosols as evapotranspiration covers on potash tailings piles.

The covering of potash tailings piles with technosols (artificial soils) is a modern and promising method for decreasing the saline drainage of these piles. In this context, it is important to determine whether technosols have appropriate physical properties for crop growth. In evapotranspiration covers, physical properties, such as bulk density, particle size distribution, total porosity, proportion of large pores, and available water are particularly important because they allow for robust crop growth, which subsequently determines the evapotranspiration capacity. However, few studies have been performed to assess the physical properties of technosols and their ability to act as evapotranspiration covers on potash tailings piles. Therefore, the present study aims to evaluate the physical properties of four different technosols made of municipal solid waste incineration bottom ash and coal combustion residues installed on a potash tailings pile located in Heringen, Germany. The total porosity, infiltration capacity, particle size distribution, bulk density, wettability, water retention curve, pH, electrical conductivity, and water content were determined. The pH of the technosols averaged 8.5, the electrical conductivity varied from 2.8 to 3.3 mS/cm, the mean bulk density was 1.21 g/cm³, the total porosity was 52.8%, and the rate of medium pores was 13.9% of the technosol volume. On average, the coarse fraction accounted for 42% of the technosol mass, whereas the fine fraction accounted for 52% of the sand-size particles, 43% of the silt-size particles and 5% of the clay-size particles. Likewise, no wetting restrictions for the technosols were found. To conclude, the different technosols present no limitations for crop growth, although the heavy metal contents of municipal solid waste incineration bottom ash and coal combustion residues should be considered in future studies.

Methane Bubble Growth and Migration in Aquatic Sediments Observed by X-ray µCT.

Methane bubble formation and transport is an important component of biogeochemical carbon cycling in aquatic sediments. To improve understanding of how sediment mechanical properties influence bubble growth and transport in freshwater sediments, a 20-day laboratory incubation experiment using homogenized natural clay and sand was performed. Methane bubble development at high resolution was characterized by µCT. Initially, capillary invasion by microbubbles (<0.1 mm) dominated bubble formation, with continued gas production (4 days for clay; 8 days for sand), large bubbles formed by deforming the surrounding sediment, leading to enhanced of macropore connectivity in both sediments. Growth of large bubbles (>1 mm) was possible in low shear yield strength sediments (<100 Pa), where excess gas pressure was sufficient to displace the sediment. Lower within the sand, higher shear yield strength (>360 Pa) resulted in a predominance of microbubbles where the required capillary entry pressure was low. Enhanced bubble migration, triggered by a controlled reduction in hydrostatic head, was observed throughout the clay column, while in sand mobile bubbles were restricted to the upper 6 cm. The observed macropore network was the dominant path for bubble movement and release in both sediments.

Water balance assessment of different substrates on potash tailings piles using non-weighable lysimeters.

Water balance is an important tool to evaluate water deficit or excess in crop systems. However, few studies have evaluated the water balance of vegetation grown on the residues from potash mining because the high sodium chloride levels of the residues hinder agricultural development. Therefore, this study aims to measure the water balance components in eight non-weighing lysimeters installed on a potash tailings pile in Heringen (Werra), Germany. These lysimeters were filled with different mixtures of household waste incineration slags and coal combustion residues, resulting in 4 different substrates with two repetitions. Manual seeding was performed using 65% perennial ryegrass (Lolium perenne L.), 25% red fescue (Festuca rubra L.) and 10% Kentucky bluegrass (Poa pratensis L.). Environmental conditions were monitored using an automatic weather station; ground-level and 1-m-high rain gauges. Precipitation and drainage were recorded weekly following the initial saturation of the lysimeters. Water balance components were determined for two hydrological years based on the expression: ET (mm) = P - D, where ET = evapotranspiration, P = precipitation and D = drainage. In addition, evapotranspiration was studied using the standard FAO Penman-Monteith equation and Haude's method. The lysimeter water balance measured in 2014 revealed an actual evapotranspiration rate of 66.4% for substrate 1, 66.9% for substrate 2, 65.1% for substrate 3 and 64.1% for substrate 4. In 2015, evapotranspiration ranged from 65.7% for substrate 4 to 70.2% for substrate 1. We observed that the FAO Penman-Monteith and Haude's evapotranspiration models generally overestimated the water use of the green coverage by 67% and 23%, respectively. Our study suggests that an evapotranspiration cover for potash tailings piles may decrease brine drainage from these piles and reduce soil and water contamination.

Three-dimensional structure and cyanobacterial activity within a desert biological soil crust.

Desert biological soil crusts (BSCs) are formed by adhesion of soil particles to polysaccharides excreted by filamentous cyanobacteria, the pioneers and main producers in this habitat. Biological soil crust destruction is a central factor leading to land degradation and desertification. We study the effect of BSC structure on cyanobacterial activity. Micro-scale structural analysis using X-ray microtomography revealed a vesiculated layer 1.5-2.5 mm beneath the surface in close proximity to the cyanobacterial location. Light profiles showed attenuation with depth of 1%-5% of surface light within 1 mm but also revealed the presence of 'light pockets', coinciding with the vesiculated layer, where the irradiance was 10-fold higher than adjacent crust parts at the same depth. Maximal photosynthetic activity, examined by O2 concentration profiles, was observed 1 mm beneath the surface and another peak in association with the 'light pockets'. Thus, photosynthetic activity may not be visible to currently used remote sensing techniques, suggesting that BSCs' contribution to terrestrial productivity is underestimated. Exposure to irradiance higher than 10% full sunlight diminished chlorophyll fluorescence, whereas O2 evolution and CO2 uptake rose, indicating that fluorescence did not reflect cyanobacterial photosynthetic activity. Our data also indicate that although resistant to high illumination, the BSC-inhabiting cyanobacteria function as 'low-light adapted' organisms.

Bridging Microbial Functional Traits With Localized Process Rates at Soil Interfaces.

In this review, we introduce microbially-mediated soil processes, players, their functional traits, and their links to processes at biogeochemical interfaces [e.g., rhizosphere, detritusphere, (bio)-pores, and aggregate surfaces]. A conceptual view emphasizes the central role of the rhizosphere in interactions with other biogeochemical interfaces, considering biotic and abiotic dynamic drivers. We discuss the applicability of three groups of traits based on microbial physiology, activity state, and genomic functional traits to reflect microbial growth in soil. The sensitivity and credibility of modern molecular approaches to estimate microbial-specific growth rates require further development. A link between functional traits determined by physiological (e.g., respiration, biomarkers) and genomic (e.g., genome size, number of ribosomal gene copies per genome, expression of catabolic versus biosynthetic genes) approaches is strongly affected by environmental conditions such as carbon, nutrient availability, and ecosystem type. Therefore, we address the role of soil physico-chemical conditions and trophic interactions as drivers of microbially-mediated soil processes at relevant scales for process localization. The strengths and weaknesses of current approaches (destructive, non-destructive, and predictive) for assessing process localization and the corresponding estimates of process rates are linked to the challenges for modeling microbially-mediated processes in heterogeneous soil microhabitats. Finally, we introduce a conceptual self-regulatory mechanism based on the flexible structure of active microbial communities. Microbial taxa best suited to each successional stage of substrate decomposition become dominant and alter the community structure. The rates of decomposition of organic compounds, therefore, are dependent on the functional traits of dominant taxa and microbial strategies, which are selected and driven by the local environment.

The impact of chemical pollution on the resilience of soils under multiple stresses: A conceptual framework for future research.

Soils are faced with man-made chemical stress factors, such as the input of organic or metal-containing pesticides, in combination with non-chemical stressors like soil compaction and natural disturbance like drought. Although multiple stress factors are typically co-occurring in soil ecosystems, research in soil sciences on this aspect is limited and focuses mostly on single structural or functional endpoints. A mechanistic understanding of the reaction of soils to multiple stressors is currently lacking. Based on a review of resilience theory, we introduce a new concept for research on the ability of polluted soil (xenobiotics or other chemical pollutants as one stressor) to resist further natural or anthropogenic stress and to retain its functions and structure. There is strong indication that pollution as a primary stressor will change the system reaction of soil, i.e., its resilience, stability and resistance. It can be expected that pollution affects the physiological adaption of organisms and the functional redundancy of the soil to further stress. We hypothesize that the recovery of organisms and chemical-physical properties after impact of a follow-up stressor is faster in polluted soil than in non-polluted soil, i.e., polluted soil has a higher dynamical stability (dynamical stability=1/recovery time), whereas resilience of the contaminated soil is lower compared to that of not or less contaminated soil. Thus, a polluted soil might be more prone to change into another system regime after occurrence of further stress. We highlight this issue by compiling the literature exemplarily for the effects of Cu contamination and compaction on soil functions and structure. We propose to intensify research on effects of combined stresses involving a multidisciplinary team of experts and provide suggestions for corresponding experiments. Our concept offers thus a framework for system level analysis of soils paving the way to enhance ecological theory.

Influence of non-invasive X-ray computed tomography (XRCT) on the microbial community structure and function in soil.

In this study the influence of X-ray computed tomography (XRCT) on the microbial community structure and function in soils has been investigated. Our results clearly indicate that XRCT of soil samples has a strong impact on microbial communities and changes structure and function significantly due to the death of selected microbial groups as a result of the treatment.