Presence and quantity of antibodies after vaccination «Gam-COVID-Vac¼.

The determine is to study the presence and quantity of specific antibodies in individuals vaccinated against COVID-19. A study of 37 blood serums of healthy adults vaccinated against COVID-19 with the vaccine «Gam-COVID-Vac¼ (or «Sputnik V¼, National Research Center of Epidemiology and Microbiology named after N.F. Gamalei) was conducted. Blood sampling was carried out 42 days after the introduction of the first component of the vaccine, and 3, 4, 5, 6 months after the end of the full course of vaccination. The presence and quantity of total IgM/IgG antibodies in the blood serum was determined by immunochemiluminescence analysis on an automatic immunochemical analyzer «Cobas e 411¼ («Roche Diagnostics¼, Germany). The results were processed by modern statistical methods. 42 days after the introduction of the first component of the vaccine «Gam-COVID-Vac¼ AT was developed in all study participants, the values of their number were variable and ranged from 36,43 to 265,43 BAU/ ml. 3, 4, 5 and 6 months after the end of the full course of vaccination AT were detected in all study participants. 6 months after the end of the full course of vaccination the number antibodies decreased by 28,16%.

Diagnostic characteristics of serological tests for the detection of SARS-CoV-2 cases.

To study the diagnostic characteristics of test systems for detecting antibodies to SARS-Cov-2. We studied the diagnostic characteristics of two test systems for detecting antibodies to SARS-Cov-2, registered in the Russian Federation. The first test system is a kit for detecting total antibodies to SARS-Cov-2 using immunochemiluminescence analysis on the «Cobas e 411¼ analyzer («Roche Diagnostics¼, Germany). The second test system is a kit for detecting IgM and IgG to SARS-Cov-2 («Core Technology Co., Ltd¼, China) by immunochromatographic analysis. The biological material for the study was blood serum. We assessed: diagnostic sensitivity, diagnostic specificity, and predictive value of positive and negative results. In the test system for detecting total antibodies to SARS-CoV-2, using an IHLA, the diagnostic sensitivity and specificity were 100%; the predictive value of positive and negative results was 100%. In the test system for the detection of IgM and IgG to Sars-CoV-2, using IHA, diagnostic sensitivity for IgM and IgG were 100%; diagnostic specificity for IgM - 60%, for IgG - 72%; predictive value of a positive result for IgM - 60%, IgG - 68,18%; predictive value of negative results for IgM and IgG - 100%. The best diagnostic characteristics were found in the test system for the detection of total antibodies to SARS-Cov-2, which must be taken into account when deciding whether to purchase test systems for the detection of antibodies to SARS-Cov-2.

[Down's syndrome with congenital acute leukemia and congenital heart disease]. / Sluchai sindroma Dauna s vrozhdennym ostrym leikozom i vrozhdennym porokom serdtsa.

OBJECTIVE: To report a rare case of Down's syndrome with congenital acute leukemia and congenital heart disease. MATERIAL AND METHODS: We have retrospectively analyzed an electronic database of patients aged 0-17 years who underwent inpatient treatment of congenital heart disease at the Federal Center for Cardiovascular Surgery for the period from January 1, 2010 to December 31, 2018. Incidence of Down's syndrome in children with congenital heart defects was 5.36% of the total number of children with congenital heart defects. No gender differences were identified. The most common congenital heart defects were ventricular septal defect, atrioventricular septal defect, atrial septal defect, patent ductus arteriosus, tetralogy of Fallot. Down's syndrome and congenital heart disease were diagnosed prenatally in 33 out of 319 patients. A rare case of Down's syndrome combined with congenital heart disease and acute leukemia is described. Treatment outcome was unfavorable. CONCLUSION. S: Evere concomitant diseases in a child with Down's syndrome determine high postoperative morbidity and mortality. Prenatal diagnosis of Down's syndrome via screening of pregnant women for ultrasound and biochemical markers of this pathology is required.

Comparative analysis of the impact of blood collection systems on the results of the study of indicators hemostasis system.

Comparative analysis of the impact of blood collection systems on the results of routine coagulation tests. The aim of this study was to conduct comparative analysis of the effects of blood collection systems on the results of routine coagulation tests. The study involved practically healthy women over 18 years of age female (n=30). Inclusion criteria: practically healthy individuals. Exclusion criteria: taking non-steroidal anti-inflammatory drugs, or oral contraceptives. Blood collection was carried out in the following systems: 1 system-Vacuette Premium,2 system- Improvacuter, 3 system- BD Vacutainer® Plus. Before and after centrifugation, visual assessment of tubes was performed.No signs for the presence of clots, hemolysis, or insufficient filling was observed. In each sample we measured prothrombin time (PV, sec), activated partial thromboplastin time (APTT, sec), and fibrinogen (F, g/l) by clotting method on the automatic coagulometer «Sta Compact¼ («Diagnostica Stago¼, France). The results of laboratory analyses were processed using parametric and non - parametric statistics using The Statistica 12.0 software. At the preanalytical laboratory stage, there were no differences when using systems for blood sampling: the clots and hemolysis were not detected in the samples, the level of filling of the sistems was sufficient. Comparative analysis revealed small, though statistically significant, differences for: PT when comparing Improvacuter wiht Vacuette Premium or BD Vacutainer®; APPT when comparing Improvacuter wiht Vacuette Premium. No differences were observed for Fg levels. Our results support the data available in the literature on the effects of blood-taking systems on hemostasis , which should be taken into account when purchasing blood collection systems.

[Improving the quality of hematological studies using sigmametry.]

The purpose - evaluated the quality of the implementation of hematology studies using sigmametry. Studies were perfomed at the Federal state budgetary establishment "Federal center cardiovascular surgery". The data were collected in the period for 2017 y. The following hematology parameters were monitored daily: white cells, red blood cell, platelet, hemoglobin. For conducting laboratory quality control we used certified three-level (Level 1 (low) , Level 2 (normal)and Level 3 (High)) control material "CBC-XE Hematology control" ("R and D Systems", USA). The study of blood hematology parameters was carried out on an automatic hematology analyzer "Sysmex XT- 2000i" and "Sysmex XT- 1800i" ("Sysmex Corporation", Japan). Sigmametry values were calculated the CV (coefficient of variation), the systematic error (Bias) and the total allowable analytical error (TE max). On the analyzer «Sysmex XT- 2000i¼ sigma values for platelet were between 3 and 4. For white blood cells, red blood cells, hemoglobin the value of the sigma was greater than 4. On the analyzer «Sysmex XT- 1800i¼ for platelet, white blood cells, red blood cells, hemoglobin the value of the sigma was greater than 4. Based on the results obtained, we drew conclusions on the choice of control material and the frequency of the internal laboratory quality control. The introduction of sigmametry in the daily activities of clinic laboratory will improve the quality of the determination of platelet, which requires further observation.

[ANALYSIS OF POPULATION IMMUNITYAGAINST INFLUENZA PRIOR TO 2014 AND 2015 EPIDEMIC SEASONS].

AIM: Control for the population herd immunity against seasonal influenza viruses as well as for emergence of antibodies against influenza with pandemic potential in human blood sera. MATERIALS AND METHODS: HAI reaction against vaccine and epidemic influenza viruses as well as HPAI viruses A/rook/Chany/32/2015 (H5N1) (clade 2.3.2. lc.) andA/Anhui/01/2013 (H7N9). RESULTS: Among all the sera samples collected in the autumn of 2014 and 2015, none had reacted in HAI against A(H5N 1) and A(H7N9) antigens even at 1:10 dilution. Among samples collected in autumn 2014, 41% were positive to A/California/07/09(H1Nlpdm9) virus, 36% - A/Texas/50/2012 (H3N2), 40% - B/Brisbane/60/2008 (Vict.lin.) and 47% reacted in HAI against the B/Massachusetts/2/2012 (Yam.lin.) strain. 22% of all the samples had a titer of at least 40 against all the antigens and only 10% in HAI had a titer of 40 or more against all the vaccine strains. Among the samples collected in autumn 2015, the number of seropositive against A/California/07/09(HlNlpdmO9) varied from 31% in the Urals FD to 46% in the Southern FD. The amount of seropositive against A/Switzerland/9715293/13 (H3N2) strain was at the level of 4 - 13% in all the FDs except Urals, where this parameter was slightly above 30%. The amount of seropositive against vaccine influenza B viruses varied from 23 to 76%. Only 2% of sera had titers in HAI of 40 or above against all the vaccine strains, 29% of all the samples were seronegative. CONCLUSION: Population immunity in Russia against influenza A(H3N2) is at a very low level, thus socially significant consequences of influenza epidemics in many aspects will depend on the vaccination campaign of autumn 2016.

[The reference intervals of antithrombin III in population of the Astrakhanskaia oblast at application of automated coagulometers.]

The foreign and national professional societies of laboratory diagnostic recommend to every laboratory to develop own or substantiate available in literature reference intervals for every laboratory index. The reference interval of antithrombin III was established for adult population of the Astrakhanskaia oblast according available standards on automated coagulometers "ACL 9000" (Instrumentation Laboratory, USA) and "STA Compact" (Diagnostica Stago, France). The received data were compared with data present in instructions to reactive kits for detection of antithrombin III and reference literature. The reference interval AT III in adult population of the Astrakhanskaia oblast has no dependencies on gender and age and can be compared with reference intervals mentioned in instructions to reactive kits for detection of activity of AT III of firms producers Instrumentation Laboratory (USA) and Diagnostica Stago (France) in the reference literature. The reference intervals AT III mentioned in instructions to reactive kits produced by Instrumentation Laboratory (USA) and Diagnostica Stago (France) and in reference literature can be applied for interpretation of results of detection of activity of AT III by automated coagulometers "ACL 9000" (Instrumentation Laboratory, USA) and "STA Compact" (Diagnostica Stago, France) in adult population of the Astrakhanskaia oblast.

[THE REFERENCE VALUES OF AGGREGATION OF PLATELETS IN ADULT POPULATION OF THE ASTRAKHAN OBLAST USING AGGREGOMETER MULTIPLATE].

The modern international standards recommend each laboratory to develop or to confirm available in literature the reference intervalsfor every laboratory indicator In the Astrakhanskaia oblast, sampling of128 healthy males andfemales were examinedfor aggregation function of thrombocytes using impedance technique and applying aggregometer Multiplate ("Verum Diagnostica", Germany). The study used as inductors peptide activating receptor of thrombin; arachidonic and adenosine diphosphoric acids. The reference range of aggregation of thrombocytes with peptide activating receptor of thrombin, at aggregometer Multiplate, in healthy population of theAstrakhanskaia oblast made up to 815.2-1498.4 AU/min, with arachidonic acid--660-1341 AU/min. with adenosine diphosphoric acid--598-1120 AU/min.

[MARKERS OF SYSTEMIC INFLAMMATORY RESPONSE IN PATIENTS OPERATED FOR COMPLICATIONS OF INFECTIOUS ENDOCARDITIS].

The study of markers of systemic inflammatory response in patients with infectious endocarditis showed that at admittance they had elevated blood C-reactive protein and ferritin levels while leukocyte count and fibrinogen content remained normal. C-reactive protein and ferritin levels increased on day 1 of the postoperative period and tended to grow further on day 3 when both parameters reached maximum values; they started to decrease on day 6 These data indicate that C-reactive protein and ferritin levels can be used to characterise the postoperative conditions of the patients including manifestations of systemic inflammation and outcome of surgical treatment.

[REFERENCE RANGES OF ANTITHROMBINE III IN ASSOCIATION WITH THE USE OF A STA COMPACT AUTOMATED COAGULOMETER].

Domestic andforeign professional diagnostic communities recommend to develop new or confirm literature reference ranges for each laboratory parameter. Bearing in mind the importance of antithrombin-III levels for anticoagulation therapy, we determined their reference ranges for the adult population of the Astrakhan region and compared them with those obtained by other authors and presented in the instructions for use of commercial kits for measuring ant ithrombin-III. Our reference ranges differ from those cited in the said instructions.

2-(1-Hydroxypropyn-2-yl)-1-vinylpyrroles: the first successful Favorsky ethynylation of pyrrolecarbaldehydes.

1-Vinylpyrrole-2-carbaldehydes react with acetylene at atmospheric pressure in a NaOH/EtOH/DMSO system at 7-10 °C to afford 2-(1-hydroxypropyn-2-yl)-1-vinylpyrroles in 53-94% yield. Thus, the first base-mediated direct ethynylation of pyrrolecarbaldehydes with free acetylene under modified conditions of the Favorsky reaction has been implemented to pave an expedient route to important biomolecules containing a pyrrole ring.

[Analysis of population immunity of residents of Russian Federation regions against seasonal and highly pathogenic strains of influenza virus].

AIM: Determine the level of antibodies against socially significant types/serotypes of influenza virus in sera of individuals residing in various regions of Russia. MATERIALS AND METHODS: 1525 samples of blood sera collected in August-December 2013 in 8 regions of Russian Federation were studied in hemagglutination inhibition reaction (HAI) with antigens obtained from A/California/07/09, (H1N1)pdm09, A/Victoria/361/2011(H3N2), B/Brisbane/60/2008 (Victoria line), B/Massachusetts/2/2012 (Yamagata line), A/Commongull/Chany/2006 (H5N1), A/Anhui/01/2013 (H7N9) influenza virus strains. RESULTS: None of the blood sera samples had significant HAI titers against A/H5 and A/H7 antigens. Of all the 1525 samples, 788 (52%) were positive with A(H1N1)pdm09 antigen; 734 (48%) reacted with A(H3N2) antigen; 1010 (66%) samples were positive with B/Victoria antigen and 602 (39%) samples were positive with B/Yamagata antigen. CONCLUSION: Healthcare institutions should pay attention to the correction of population immunity profile in regions for the reduction of social-economic losses from seasonal influenza epidemics.

[The importance of thrombocytic indices in patients operated for chronic rheumatic heart disease].

We studied the importance of thrombocytic indices in patients operated for chronic rheumatic heart disease and showed that a decrease in the platelet count is accompanied by increased thrombocytic indices including platelet width and mean volume, the relative number of large platelets in peripheral blood The increase was regarded as a compensatory response to the decrease in the platelet count; it suggests enhanced thrombocytopoiesis. It is concluded that deretmination of thrombocytic indices in cardiosurgical patients using automated hematological analyzers allows to evaluate the prcesses of thrombocytopoiesis.

[Changes in biochemical parameters of blood in patients with rheumatic heart failure following surgical intervention].

We analysed changes of biochemical parameters of blood in patients treated for chronic rheumatic heart problems. It was shown that biochemical parameters of blood changed within 12 days after surgical intervention; the changes were especially pronounced on days 1-3 whereas normalization occurs by days 9-12. Pathogenetically sound time periods for changes of biochemical characteristics of blood serum were established in the patients treated for chronic rheumatic heart problems. On the one hand, it will allow more accurate monitoring of development of complications in the postoperative period and on the other hand to reduce expenses by decreasing the number of unnecessary analyses.

[The significance of immature granulocytes in diagnostic of infectious inflammatory processes in cardiosurgery patients].

The evaluation was implemented concerning the values of automated parameter "immature granulocytes" in cardiosurgery patients. It is demonstrated that amount of immature granulocytes in peripheral blood exceeding 2% indicates development of infectious inflammatory process (sepsis). The monitoring of amount of immature granulocytes in cardiosurgery patients makes it possible to evaluate effectiveness of applied therapy, severity of patient condition, course of post-surgery period and outcome of disease.

Fluorescence from 3,5-diphenyl-8-CF3-BODIPYs with amino substituents on the phenyl rings: Quenching by aromatic molecules.

The photoluminescence from n-hexane solutions of the dyes obtained by the introduction of amino groups into the meta position of the phenyl rings of 3,5-diphenyl-8-CF3-BODIPY was found to be strongly quenched by some aromatic molecules (benzene and toluene). On the contrary, the introduction of an amino group into the para position does not lead to significant quenching. The quenching of photoluminescence from the meta derivatives obeys the mixed static + dynamic mechanism. Temperature-dependent time-resolved fluorescence measurements were performed to determine the Stern-Volmer constants of the static and dynamic components of quenching. It follows from these data that the binding energy between luminophore and toluene molecules is about 5.0 kcal/mol in the ground state and larger than 3.4 kcal/mol in the excited state. Complexation with toluene facilitates the intramolecular charge transfer in the BODIPY derivatives with the meta position of the amino groups, resulting in photoluminescence quenching.

[Type of attitude towards the disease, quality of life, and treatment compliance during essential hypertension debut].

Quality of life (QL), types of attitude towards the disease (TATD), and treatment compliance (TC) were studied in 36 men aged 18 to 59 years, who had had the diagnosis of arterial hypertension (AH) for 1 to 3 years. Type of Attitude Towards Disease Questionnaire (Bekhterev Research Institute, 1987) and QL of AH Patients Questionnaire (Ivanovo State Medical Academy, 2000) were used. The study revealed that QL of the patients and their TC depended on TATD. At AH debut in men, anosognostic, ergopathic, and anosognostico-ergopathic TATD prevail; QL of these men is equal to that of practically healthy persons; the absence of TC is associated with these TATD. Harmonic TATD, which took place in 8% of the patients, facilitated TC. In 20% patients, TATD included hypochondrial, anxious, and sensitive components, which prevented TC and formed the lowest QL.

[Life quality in patients with early hypertensive disease]. / Kachestvo zhizni bol'nykh s nachal'noi stadiei gipertonicheskoi bolezni.

Thirty-seven males aged 18 to 55 years (mean age 29.6 +/- 1.8 years) who had a 1-3-year history of arterial hypertension (AH) were examined. All the patients were found to have grade 1 hypertension. Life quality (LQ) was studied using the questionnaire developed by the Ivanovo State Medical Academy in 2000. A significant decrease in LQ was found at the peak of AH in the patients as compared with healthy individuals; however, the specific parameters of the health status and reductions in their functional capacities differed slightly. It is concluded that it is necessary to apply a standardized approach to assessing the patients' complaints and medical history data.