The perception of silence.

Auditory perception is traditionally conceived as the perception of sounds-a friend's voice, a clap of thunder, a minor chord. However, daily life also seems to present us with experiences characterized by the absence of sound-a moment of silence, a gap between thunderclaps, the hush after a musical performance. In these cases, do we positively hear silence? Or do we just fail to hear, and merely judge or infer that it is silent? This longstanding question remains controversial in both the philosophy and science of perception, with prominent theories holding that sounds are the only objects of auditory experience and thus that our encounter with silence is cognitive, not perceptual. However, this debate has largely remained theoretical, without a key empirical test. Here, we introduce an empirical approach to this theoretical dispute, presenting experimental evidence that silence can be genuinely perceived (not just cognitively inferred). We ask whether silences can "substitute" for sounds in event-based auditory illusions-empirical signatures of auditory event representation in which auditory events distort perceived duration. Seven experiments introduce three "silence illusions"-the one-silence-is-more illusion, silence-based warping, and the oddball-silence illusion-each adapted from a prominent perceptual illusion previously thought to arise only from sounds. Subjects were immersed in ambient noise interrupted by silences structurally identical to the sounds in the original illusions. In all cases, silences elicited temporal distortions perfectly analogous to the illusions produced by sounds. Our results suggest that silence is truly heard, not merely inferred, introducing a general approach for studying the perception of absence.

Experimental comparison of E-band BDFA and Raman amplifier performance over 50 km G.652.D fiber using 30 GBaud DP-16-QAM and DP-64-QAM signals.

We compare the performance of three optical amplifiers in the E-band: a bismuth-doped fiber amplifier (BDFA), a distributed Raman amplifier, and a discrete Raman amplifier (RA). Data transmission performance of 30 GBaud DP-16-QAM and DP-64-QAM signals transmitted over 50 km of G.652.D fiber is compared in terms of achieved signal-to-noise (SNR). In this specific case of relatively short distance, single-span transmission, the BDFA outperforms the distributed and discrete Raman amplifiers due to the impact of fiber nonlinear penalties at high input signal powers.

Living with trimethylaminuria and body and breath malodour: personal perspectives.

BACKGROUND: Many people suffer from body and breath malodour syndromes. One of these is trimethylaminuria, a condition characterized by excretion in breath and bodily fluids of trimethylamine, a volatile and odorous chemical that has the smell of rotting fish. Trimethylaminuria can be primary, due to mutations in the gene encoding flavin-containing monooxygenase 3, or secondary, due to various causes. To gain a better understanding of problems faced by United Kingdom residents affected by body and breath malodour conditions, we conducted a survey. METHODS: Two anonymous online surveys, one for adults and one for parents/guardians of affected children, were conducted using the Opinio platform. Participants were invited via a trimethylaminuria advisory website. Questions were a mix of dropdown, checkbox and open-ended responses. Forty-four adults and three parents/guardians participated. The dropdown and checkbox responses were analysed using the Opinio platform. RESULTS: All participants reported symptoms of body/breath odour. However, not all answered every question. Twenty-three respondents experienced difficulties in being offered a diagnostic test for trimethylaminuria. Problems encountered included lack of awareness of the disorder by medical professionals and reluctance to recognise symptoms. Of those tested, 52% were diagnosed with trimethylaminuria. The main problems associated with living with body/breath malodours were bullying, harassment and ostracism in either the workplace (90%) or in social settings (88%). All respondents thought their condition had disadvantaged them in their daily lives. Open-ended responses included loss of confidence, stress, exclusion, isolation, loneliness, depression and suicidal thoughts. Respondents thought their lives could be improved by greater awareness and understanding of malodour conditions by medical professionals, employers and the general public, and appreciation that the malodour was due to a medical condition and not their fault. CONCLUSIONS: Breath and body malodour conditions can cause immense hardship and distress, both mentally and socially, having devastating effects on quality of life. It would be advantageous to establish a standardised pathway from primary care to a specialist unit with access to a robust and reliable test and diagnostic criteria. There is a need to recognise malodour disorders as a disability, giving affected individuals the same rights as those with currently recognised disabilities.

The effect of weather conditions on scores at the United States Masters golf tournament.

This paper investigates the sensitivity of golfers' performance to meteorological conditions at the men's US Masters tournament over the 40-year period 1980-2019. The mean and standard deviation of round scores are related to local temperature, humidity, wind speed and direction, and concurrent and antecedent precipitation. Mean scores are more dependent on weather conditions than the variability of scores in a given round. The best predictor of mean scores is the wet-bulb temperature in rounds one and two, and the zonal wind speed in rounds three and four. Across both sets of rounds (1 and 2, and 3 and 4), the wet-bulb temperature is a better predictor of mean scores than the air temperature, which implies that atmospheric moisture content affects scores. In general, golfers take fewer shots and so perform better in warmer and calmer conditions. The synergestic effect of several weather variables explains over 44% of the variance in mean scores. Mean meteorological conditions during play are a much better predictor of the players' average performance than the standard deviation of the weather variables. The golfers' performance becomes more variable in cooler conditions with a wider range of scores. Precipitation during play and the dampness of the ground (as quantified by rainfall up to ten days before play) do not have a consistent and statistically significant effect on the competitors' performance. In short, this paper demonstrates that golf scores are dependent on weather conditions.

Performance on stochastic figure-ground perception varies with individual differences in speech-in-noise recognition and working memory capacity.

Speech recognition in noisy environments can be challenging and requires listeners to accurately segregate a target speaker from irrelevant background noise. Stochastic figure-ground (SFG) tasks in which temporally coherent inharmonic pure-tones must be identified from a background have been used to probe the non-linguistic auditory stream segregation processes important for speech-in-noise processing. However, little is known about the relationship between performance on SFG tasks and speech-in-noise tasks nor the individual differences that may modulate such relationships. In this study, 37 younger normal-hearing adults performed an SFG task with target figure chords consisting of four, six, eight, or ten temporally coherent tones amongst a background of randomly varying tones. Stimuli were designed to be spectrally and temporally flat. An increased number of temporally coherent tones resulted in higher accuracy and faster reaction times (RTs). For ten target tones, faster RTs were associated with better scores on the Quick Speech-in-Noise task. Individual differences in working memory capacity and self-reported musicianship further modulated these relationships. Overall, results demonstrate that the SFG task could serve as an assessment of auditory stream segregation accuracy and RT that is sensitive to individual differences in cognitive and auditory abilities, even among younger normal-hearing adults.

The predictive value of the sFlt-1/PlGF ratio in suspected preeclampsia in a New Zealand population: A prospective cohort study.

BACKGROUND: Internationally, placental growth factor (PlGF)-based tests are used as prognostic markers in suspected preeclampsia. However, Ministry of Health guidelines do not currently endorse PlGF-based tests in New Zealand (NZ). AIMS: To investigate the predictive value of soluble fms-like tyrosine kinase 1 (sFlt-1)/PlGF ratio in suspected preeclampsia in a NZ population. MATERIALS AND METHODS: A prospective cohort study of singleton pregnancies at 20+0 -36+6 weeks gestation with suspected preeclampsia as defined by Society of Obstetric Medicine Australia and NZ (SOMANZ) criteria. PRIMARY OBJECTIVE: to evaluate a sFlt-1/PlGF ratio >38 at ≤35+0 weeks gestation to predict birth ≤14 days. SECONDARY OBJECTIVES: to assess a sFlt-1/PlGF ratio cut-off of 38 at ≤37+0 weeks gestation, to rule out preeclampsia ≤1 week, rule in preeclampsia ≤4 weeks, and to predict perinatal outcome. Clinicians were blinded to sFlt-1/PlGF ratio results. RESULTS: Included were 222 participants, 19.4% Maori and 10.4% Pasifika. A sFlt-1/PlGF >38 predicted birth ≤14 days, positive predictive value (PPV) 51.4% (95% CI, 39.6-63.0) and negative predictive value (NPV) 95.9% (95% CI, 91.4-98.1), median (interquartile range) days to birth 14 (2-27) vs 49 (33-70), P < 0.000. A sFlt-1/PlGF cut-off of 38 ruled out preeclampsia ≤1 week (NPV 96.2% (95% CI, 92.3-98.2)) and ruled in preeclampsia ≤4 weeks (PPV 75.0% (95% CI, 65.0-82.9)). A sFlt-1/PlGF >38 was associated with greater perinatal morbidity. CONCLUSIONS: The predictive value of the sFlt-1/PlGF ratio in NZ is comparable to that reported in international trials. Used in clinical practice the sFlt-1/PlGF ratio may aid risk stratification in suspected preeclampsia, directing limited resources to those pregnancies at highest risk.

Performance evaluation of discrete Raman amplifiers in coherent transmission systems.

We evaluate the performance penalty due to discrete Raman amplifier (DRA) in a long haul WDM transmission system. The investigation was primarily performed to study the impact of the accumulated nonlinear noise due to fibre chromatic dispersion and nonlinear coefficient(γ). Nonlinear fibres such as inverse dispersion fibre (IDF), dispersion compensation fibre (DCF) and a development fibre known as the Corning Raman fibre (CRF) with the opposite sign of CD to the other two, were taken as the gain fibre in the DRA stage of the long-haul transmission setup. To study the performance penalty with these Raman gain fibres a 30 GBaud 120 Gb/s DP-QPSK channel @1550 nm was combined with 9 spectrally shaped 50 GHz amplified spontaneous emission (ASE) channels for transmission over a recirculation loop with a per loop length of 63 km single mode fibre (SMF). Our modelling and experimental results show that a fibre with positive dispersion >10ps/nm/km and a nonlinear coefficient of ∼ 4W-1km-1 is a good choice of gain fibre for DRA-assisted coherent transmission system.

E-, S-, C- and L-band coherent transmission with a multistage discrete Raman amplifier.

We report for the first time an ultra-wideband coherent (UWB) WDM transmission over a 70 km standard single mode fibre (SSMF) solely using a multistage discrete Raman amplifier (DRA) over the E-, S-, C- and L-bands of the optical window. The amplifier is based on a split-combine approach of spectral bands enabling signal amplification from 1410-1605 nm over an optical bandwidth of 195 nm (25.8 THz). The proposed amplifier was characterized with 143 channelized amplified spontaneous emission (ASE) dummy channels in the S-, C- and L-bands and 4 laser sources in the E-band (1410-1605 nm). The amplification results show an average gain of 14 dB and a maximum noise figure (NF) of 7.5 dB over the entire bandwidth. Coherent transmission with the proposed amplifier was performed using a 30 Gbaud PM-16-QAM channel coupled with the ASE channels over a 70 km SMF. The ultra-wideband transmission using the tailored multistage DRA shows transmission bandwidth of 195 nm with a maximum Q2 penalty of ∼4 dB in E- and S-band, and ∼2 dB in C- and L-band.

30-GBaud DP 16-QAM transmission in the E-band enabled by bismuth-doped fiber amplifiers.

We report the transmission of five 30-GBaud dual polarization 16-QAM signals over 160 km of standard single-mode fiber in the E-band (1410-1460 nm). The transmission line consists of two 80-km spans and three independent bismuth-doped fiber amplifiers. The developed amplifiers feature a maximum gain of 27.3 dB, 33.8 dB, and 28.3 dB with a minimum noise figure of 4.8 dB, 4.7 dB, and 5.3 dB, respectively. The maximum signal Q2 factor penalty is 4.5 dB, and the overall performance of the system is above the pre-forward-error-correction (FEC) threshold for a 10-15 post-FEC bit error rate. To the best of our knowledge, this is the record experimentally demonstrated transmission length for a coherent detection signal in the E-band.

Ultra-wideband discrete Raman amplifier optimization for single-span S-C-L-band coherent transmission systems.

We experimentally compare the performance of two key ultra-wideband discrete Raman amplifier structures, a cascaded dual-stage structure and an in-parallel dual-band structure, in fully loaded S-C-L band coherent transmission systems over 70 km of single-mode fiber. Our results show that dual-band discrete Raman amplifier with minimized backreflections can effectively avoid unstable random distributed feedback lasing, reduce the noise figure, and therefore improve the transmission performance for signals at shorter wavelengths, versus the cascaded dual-stage structure. The average noise figure for S-band signals is 6.8 dB and 7.2 dB for the dual-band structure and cascaded dual-stage structure, respectively, while the average S-band Q2 factor is similarly improved by 0.6 dB. Moreover, the cascaded dual-stage discrete Raman amplifier requires guard bands around the 1485-nm and 1508-nm pumps as the signal and pump wavelengths overlap, which results in a bandwidth loss of ∼10 nm and reduces the potential net data throughput to 28.6 Tb/s for 30-GBaud DP-16QAM signals. However, the dual-band structure can utilize the bandwidth more effectively, which leads to a higher estimated net data throughput of 31.2 Tb/s.

Transcutaneous Auricular Vagus Nerve Stimulation Strengthens Semantic Representations of Foreign Language Tone Words during Initial Stages of Learning.

Difficulty perceiving phonological contrasts in a second language (L2) can impede initial L2 lexical learning. Such is the case for English speakers learning tonal languages, like Mandarin Chinese. Given the hypothesized role of reduced neuroplasticity in adulthood limiting L2 phonological perception, the current study examined whether transcutaneous auricular vagus nerve stimulation (taVNS), a relatively new neuromodulatory technique, can facilitate L2 lexical learning for English speakers learning Mandarin Chinese over 2 days. Using a double-blind design, one group of participants received 10 min of continuous priming taVNS before lexical training and testing each day, a second group received 500 msec of peristimulus (peristim) taVNS preceding each to-be-learned item in the same tasks, and a third group received passive sham stimulation. Results of the lexical recognition test administered at the end of each day revealed evidence of learning for all groups, but a higher likelihood of accuracy across days for the peristim group and a greater improvement in response time between days for the priming group. Analyses of N400 ERP components elicited during the same tasks indicate behavioral advantages for both taVNS groups coincided with stronger lexico-semantic encoding for target words. Comparison of these findings to pupillometry results for the same study reported in Pandza, N. B., Phillips, I., Karuzis, V. P., O'Rourke, P., and Kuchinsky, S. E. (Neurostimulation and pupillometry: New directions for learning and research in applied linguistics. Annual Review of Applied Linguistics, 40, 56-77, 2020) suggest that positive effects of priming taVNS (but not peristim taVNS) on lexico-semantic encoding are related to sustained attentional effort.

RIN induced penalties in G.654.E and G.652.D based distributed Raman amplifiers for coherent transmission systems.

Relative intensity noise (RIN) induced penalties were experimentally measured in distributed Raman amplifiers (DRAs) for G.654.E and G.652.D fibres with forward, backward and bidirectional pumping configurations. The measured signal RIN using the G.654.E fibre was ∼3.5 dB and ∼2 dB lower than the G.652.D fibre with forward (FW) pump configuration for PM-QPSK and PM-8QAM signals, with single span transmission showing a Q-factor improvement of ∼3 dB and ∼2.5 dB for G.654.E over G.652.D fibres. The performance penalty in a long haul coherent system was evaluated for 28 GBaud PM-QPSK signals using a recirculation loop for backward and bidirectional distributed Raman amplifiers. Our experimental results demonstrate an additional transmission distance of more than 1000 km for G.654.E over its counterpart G.652.D assuming a HD-FEC limit of 8.5 dB.

Material jet printing of transparent ceramic Yb:YAG planar waveguides.

A new, to the best of our knowledge, 3D additive manufacturing technique utilizing particle-loaded ink jet printing to fabricate transparent ceramic Yb:YAG planar waveguides for laser gain media was demonstrated. Rheological optimization of YAG particle-loaded inks resulted in successful droplet formation and printing resolution. Planar waveguides composed of a Yb:YAG guide encased in undoped YAG cladding were printed with guide thicknesses ranging between 25 and 350 µm and consolidated to high optical quality via solid-state sintering. Sufficiently low optical (1-3%/cm) and intermodal scatter allowed single-mode propagation with a core/clad index difference of $\Delta {n}\sim{5.0} \times {{10}^{- 4}}$ (corresponding to 3 at.% Yb). The waveguides were cladding-pumped longitudinally with a 940 nm diode array resulting in 23.6% slope efficiency in 2 ms pulsed operation.

Raman Amplification Optimization in Short-Reach High Data Rate Coherent Transmission Systems.

We compared the transmission performances of 600 Gbit/s PM-64QAM WDM signals over 75.6 km of single-mode fibre (SMF) using EDFA, discrete Raman, hybrid Raman/EDFA, and first-order or second-order (dual-order) distributed Raman amplifiers. Our numerical simulations and experimental results showed that the simple first-order distributed Raman scheme with backward pumping delivered the best transmission performance among all the schemes, notably better than the expected second-order Raman scheme, which gave a flatter signal power variation along the fibre. Using the first-order backward Raman pumping scheme demonstrated a better balance between the ASE noise and fibre nonlinearity and gave an optimal transmission performance over a relatively short distance of 75 km SMF.

High-capacity multi-span transmission performance characterization of broadband discrete Raman amplifier.

The performance of a multi-span transmission link compensated with a >75nm broadband discrete Raman amplifier is experimentally evaluated using multiple DP-x-QAM modulation formats over a multi-channel C + L band WDM grid with up to 182 × 50 GHz spaced channels.

Impact of pump-signal overlap in S+C+L band discrete Raman amplifiers.

We experimentally investigate the impact of pump-signal overlap in ultra-wideband (>13THz) Raman amplifiers and measure the transmission penalty on 30GBaud PM-QPSK signals due to adjacent Raman pumps in a 15dB gain, 150nm (∼18.8THz) S+C+L-band discrete Raman amplifier. We present an efficient numerical model to predict the performance penalty induced by crosstalk from Rayleigh backscattered light from backward-propagating Raman pumps showing good agreement with the experimental results. A 4nm guard-band must be retained around an overlapping Raman pump based on typical, commercial semiconductor laser pump diodes to ensure a negligible transmission penalty in S-band.

Flavin-Containing Monooxygenase 1 Catalyzes the Production of Taurine from Hypotaurine.

Taurine is one of the most abundant amino acids in mammalian tissues. It is obtained from the diet and by de novo synthesis from cysteic acid or hypotaurine. Despite the discovery in 1954 that the oxygenation of hypotaurine produces taurine, the identification of an enzyme catalyzing this reaction has remained elusive. In large part, this is due to the incorrect assignment, in 1962, of the enzyme as an NAD-dependent hypotaurine dehydrogenase. For more than 55 years, the literature has continued to refer to this enzyme as such. Here we show, both in vivo and in vitro, that the enzyme that oxygenates hypotaurine to produce taurine is flavin-containing monooxygenase (FMO) 1. Metabolite analysis of the urine of Fmo1-null mice by 1H NMR spectroscopy revealed a buildup of hypotaurine and a deficit of taurine in comparison with the concentrations of these compounds in the urine of wild-type mice. In vitro assays confirmed that human FMO1 catalyzes the conversion of hypotaurine to taurine, utilizing either NADPH or NADH as cofactor. FMO1 has a wide substrate range and is best known as a xenobiotic- or drug-metabolizing enzyme. The identification that the endogenous molecule hypotaurine is a substrate for the FMO1-catalyzed production of taurine resolves a long-standing mystery. This finding should help establish the role FMO1 plays in a range of biologic processes in which taurine or its deficiency is implicated, including conjugation of bile acids, neurotransmitter, antioxidant and anti-inflammatory functions, and the pathogenesis of obesity and skeletal muscle disorders. SIGNIFICANCE STATEMENT: The identity of the enzyme that catalyzes the biosynthesis of taurine from hypotaurine has remained elusive. Here we show, both in vivo and in vitro, that flavin-containing monooxygenase 1 catalyzes the oxygenation of hypotaurine to produce taurine.

Flavin-containing monooxygenase 3 (FMO3): genetic variants and their consequences for drug metabolism and disease.

The review focuses on genetic variants of human flavin-containing monooxygenase 3 (FMO3) and their impact on enzyme activity, drug metabolism and disease.The majority of FMO-mediated metabolism in adult human liver is catalyzed by FMO3. Some drugs are metabolized in human liver predominantly by FMO3, but most drug substrates of FMO3 are metabolized also by other enzymes, particularly cytochromes P-450, and the FMO3-catalyzed reaction is not the major route of metabolism.Rare variants that severely affect production or activity of FMO3 cause the disorder trimethylaminuria and impair metabolism of drug substrates of FMO3. More common variants, particularly p.[(Glu158Lys);(Glu308Gly)], can moderately affect activity of FMO3 in vitro and reduce metabolism of drug substrates in vivo, in some cases increasing drug efficacy or toxicity.Common variants of FMO3 have been associated with a number of disorders, but additional studies are needed to confirm or refute such associations.Elevated plasma concentrations of trimethylamine N-oxide, a product of an FMO3-catalyzed reaction, have been implicated in certain diseases, particularly cardiovascular disease. However, the evidence is often contradictory and additional work is required to establish whether trimethylamine N-oxide is a cause, effect or biomarker of the disease.Genetic variants of other FMOs are also briefly discussed.

Three-year follow up of adrenal incidentalomas in a New Zealand centre.

BACKGROUND: The international guidelines for management of adrenal incidentalomas (AI) are becoming more conservative. These changes are based on the growing body of evidence suggesting that non-functioning adenomas have a low likelihood of becoming functional or malignant over time. AIMS: To follow up at least 100 patients for 3 years who were originally found to have benign adrenal adenomas which were non-functional or had subclinical Cushing syndrome (SCS). METHODS: This study prospectively evaluated consecutive patients aged 18 years or older with benign adrenal incidentalomas (AI), not treated with adrenalectomy, which were non-functioning or had SCS. The initial and follow-up evaluation, including clinical assessment, hormonal investigations and imaging were coordinated via a standardised nurse-led AI clinic. RESULTS: Of 233 patients referred to the AI clinic, 101 patients met the inclusion criteria and completed 3-year follow up. Most of those excluded were due to incomplete initial or follow-up evaluation or were not true AI. Most AI either remained stable or decreased in size on repeat imaging, while 5% of patients had AI enlargement of >5 mm diameter. No patient developed features suggesting adrenal carcinoma. Ninety-two patients had an initial diagnosis of non-functioning adenoma and nine patients had SCS. After 3 years (range 2.9-4.7 years), five of the nine patients with SCS showed normalisation of cortisol parameters (44%), and five of the 92 non-functional AI patients developed SCS (5%). CONCLUSION: After 3 years of follow up, approximately half of patients with SCS normalised, while 5% of patients with initially non-functioning adenomas developed biochemical evidence of SCS. This study found a low likelihood of progressive hormonal excess with no evidence of malignancy developing on follow-up evaluation, providing support for the shift towards the more conservative approach to management of AI recommended in recent guidelines.

Biochar amendment and water stress alter rhizosphere carbon and nitrogen budgets in bauxite-processing residue sand under rehabilitation.

Nitrogen (N) bioavailability is one of the main limiting factors for microbial activity and vegetation establishment in bauxite-processing residue sand (BRS). Although beneficial effects of biochar on reducing N loss in the early stages of BRS rehabilitation have been observed previously, the underlying mechanisms of this complicated process, particularly the interactions between applied biochar and the plant rhizosphere is largely unknown. This glasshouse study (116 days), investigated the coupled effects of biochar and water stress on N bioavailability in the rhizosphere of ryegrass (Lolium rigidum) grown in BRS amended with di-ammonium phosphate (DAP) fertiliser (at rates of 0 or 2.7 t ha-1) with and without biochar amendment. The applied biochar was characterised as either aged acidic (AC) or alkaline pine (PC) and was mixed with BRS at a rate of 5% v/v under four moisture regimes (50%, 40%, 20% and 7.5% water holding capacity). Amending BRS with AC and PC biochars increased NH4+ retention and decreased cumulative NH3 volatilization within both the rhizosphere and root-free zones compared with fertiliser only treatment. These effects were more pronounced for the AC than PC biochar, suggesting that aged acidic biochar has the great potential for use in rapid establishment of vegetation in BRS disposal areas. The biochar amendment increased cumulative nitrous oxide emissions compared with DAP only treatment, with no significant differences among different moisture regimes. The Control and 20% water holding capacity (WHC) treatment showed the highest dissolved organic carbon (DOC) concentrations compared with other treatments and moisture regimes in the ryegrass rhizosphere, while the highest dissolved organic N concentration were observed in the DAP + AC treatment. Reducing moisture levels below 20% WHC generally decreased microbial biomass carbon (MBC) concentrations and activity in both the rhizosphere and root-free zones of all treatments, while total N generally decreased as moisture levels decreased from 50% to 7.5% WHC. Plant took up more N in the DAP + AC treatment compared with DAP + PC and DAP only treatments, while increasing water stress generally resulted in decreased aboveground biomass.