Feed as a source of polybrominated diphenyl ethers (PBDEs).

One of the most important routes for human exposure to polybrominated diphenyl ethers (PBDEs) is the ingestion of contaminated food. Food of animal origin safety is strongly related to feed quality. The aim of the study was the assessment of feeds and feed materials quality associated with ten PBDE congeners (BDE-28, 47, 49, 99, 100, 138, 153, 154, 183 and 209) contamination. The quality of 207 feed samples divided into eight categories (277/2012/EU) was checked using the gas chromatography-high resolution mass spectrometry (GC-HRMS). At least one congener was identified in 73% of the samples. All investigated fish oil, animal fat, and feed for fish were contaminated, and 80% of plant-origin feed samples were free of PBDEs. The highest median content of ∑10PBDE was found in fish oils (2260 ng kg-1) followed by fishmeal (530 ng kg-1). The lowest median was found in mineral feed additives, plant materials excluding vegetable oil and compound feed. BDE-209 was the most frequently detected congener (56%). All congeners except BDE-138 and BDE-183 were detected in 100% of the fish oil samples. Except for BDE-209, the congener detection frequencies did not exceed 20% in compound feed, feed of plant origin, and vegetable oils. Excluding BDE-209, similar congener profiles were found for fish oils, fishmeal and feed for fish, with BDE-47 in the highest concentration, followed by BDE-49 and BDE-100. Another pattern appeared in animal fat, with a higher median concentration of BDE-99 than BDE-47. Time-trend analysis of PBDE concentrations in fishmeal (n = 75) showed a 63% decrease in ∑10PBDE (p = 0.077) and a 50% decrease in the ∑9PBDE (p = 0.008) between 2017 and 2021. It proves the international legislation implemented to reduce PBDE environmental levels has been effective.

Terrestrial animal livers as a source of PCDD/Fs, PCBs and PBDEs in the diet.

The livers of terrestrial animals are an important source of vitamins and minerals, but with the liver being the major filtering and detoxifying organ, it retains xenobiotic material, especially persistent organic pollutants (POPs). The aim of the study was human health risk assessment related to the presence of certain POPs, namely polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) in terrestrial animals' livers. HRGC-HRMS was used to determine analyte concentrations in 99 liver samples of chicken, pork, beef, and mutton livers obtained from Polish farms. The ovine livers were the most contaminated by ∑PCDD/F/dl-PCBs (0.06-2.21 pg WHO-TEQ g-1 wet weight), ∑ndl-PCBs (0.036-1.54 ng g-1 wet weight) and ∑PBDEs (0.21-2.27 ng g-1 wet weight). Samples were found of ovine and pork livers that exceeded the maximum concentration limits established for PCDD/Fs. The Kruskal-Wallis test showed that there were statistical differences between regions in the concentrations of the ∑PCDD/Fs and ∑PCDD/F-PCBs, but none in those of the ∑dl-PCBs, ∑ndl-PCBs or ∑PBDEs. According to Dunnett's test results, ovine liver samples from the Podlaskie province contained fewer ∑PCDD/F than those from the Dolnoslaskie (p < 0.027) and Malopolskie (p < 0.0342) voivodeships. Taking into account PCDD/F contents and Tolerable Weekly Intake (2 pg WHO-TEQ/kg BW) (TWI2018), the lowest risk was associated with chicken liver consumption (TWI up to 11 % in the 95th percentile), pork (62 %) and bovine (92 %) livers should be eaten in limited amounts for risk avoidance, while mutton liver (400 %) should not be eaten at all. Additionally, there is no risk of ingestion of a harmful amount of PBDEs associated with consumption of the livers of terrestrial animals from Poland based on the margin of exposure values (>15).

Polybrominated diphenyl ethers (PBDEs) in raw milk from different animal species and in infant formula. Occurrence and risk assessment.

Polybrominated diphenyl ethers (PBDEs) are widespread, persistent in the environment, and classified as global pollutants. Their presence has been confirmed in various types of food which adversely affect human health when consumed in sufficient amounts. Although milk has advantageous nutritional qualities and there are health benefits associated with its consumption, it could also contain toxic PBDEs. The aim of the study was the determination of the concentrations of ten congeners (BDE -28, -47, -49, -99, -100, -138, -153, -154, -183, and 209) in cow's, sheep's, and goat's milk obtained from Polish farms and their determination in infant formula. A total of 103 samples of raw milk and infant formula were tested using an accredited high-resolution gas chromatography-high-resolution mass spectrometry method. PBDEs were detected in all analyzed samples, the highest concentration being found in sheep's milk (11.9 ng g-1 fat), and cow's milk containing the least contamination. BDE-209 makes the predominant contribution to the sum of the ten congeners, constituting at least 38%. The profiles of PBDEs were dependent on the milk type and the differences between its varieties are discussed. The highest median concentration of the sum of ten PBDEs (0.473 ng g-1 fat) was determined in infant formula, which was identified as an important source of infants' exposure (5.48 ng kg-1 b.w. day-1 calculated based on P95 concentration). Milk is a source of PBDE in the diet; however, considered in isolation its consumption does not pose a risk to either adults' or children's health.

Quantification of polybrominated diphenyl ethers (PBDEs) in food. A review.

The polybrominated diphenyl ethers (PBDEs), a class of brominated flame retardants (BFRs), are food contaminants of animal origin. Interest in food matrices analysis is growing due to the toxicity of PBDEs and European Commission (EC) recommendation (118/2014/EU). Here we review papers concerning methods of PBDEs analysis while focusing on extraction, clean up, chromatographic separation and detection techniques. The emphasis is put on EC recommendation, the congeners and the efficiency of different detection systems. Some analytical problems caused by differences between low- and high-molecular-mass congener properties, especially the possible limitations of BDE-209 analysis, are discussed. Detection techniques and mass spectrometry (MS) ionization modes applied to PBDE level determination in food of animal origin are compared. The gas chromatography (GC) coupled to high-resolution MS is undoubtedly fit for that purpose, but ion trap MS could be used to PBDEs determination as well. ECD is the most sensitive technique; however, other halogen compounds present in sample may interfere with PBDEs congeners necessitating results confirmation. Moreover, the novel atmospheric pressure chemical ionization (APCI) method applied to GC in tandem with MS places this technique in the top category of the most sensitive techniques which may be used.